Tris EDTA (TE) Buffer Stock Preparation
Updated 6/12/2015 08:31 PM
Introduction
Materials
- Consumables
- EDTA stock (pH 8.0)
- Tris-Cl stock
- Disposables
- pipette tips
- Equipment
- media bottle
- pipettor
- volumetric flask
Procedure
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 100 |
2 2 | Concentration (x stock) | 10 |
Table2
A A | B B | |
1 1 | Stock Solution | Concentration (M)* |
2 2 | EDTA (pH 8.0) | 0.5 |
3 3 | Tris-Cl | 1 |
Table3
A A | B B | |
1 1 | Component | Volume (mL) |
2 2 | EDTA (pH 8.0) stock | 2 |
3 3 | Tris-Cl stock | 10 |
4 4 | dH2O | 88 |
Preparation
- Add the calculated amount of dH2O to a sterile media bottle.
Generally, the capacity of the bottle should be greater than (not equal to) the final volume of media to be prepared in order to prevent the media from boiling over during the autoclave step.
- Add the calculated amounts of the EDTA and Tris-Cl stock solutions.
- Apply autoclave tape to the bottle and autoclave on liquids cycle.
CRITICALLiquids cycle.
- Store at room temperature.
This protocol describes the preparation of a concentrated Tris EDTA (TE) buffer. It was adapted from Sambrook & Russel.
Note: The overall pH of the buffer is dictated by the pH value of the Tris-Cl solution, the EDTA solution should always be pH 8.0.
Sambrook, J. & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual, 3 edn. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. pp. A1.7