EDTA Stock Preparation
Updated 6/12/2015 07:31 PM
Introduction
Materials
- Consumables
- EDTA disodium salt dihydrate
- NaOH
- Disposables
- weigh dishes/paper
- Equipment
- balance
- autoclave
- sterile container
Procedure
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 1000 |
2 2 | Final Concentration (M)* | 0.5 |
Table3
A A | B B | |
1 1 | Component | Weight (g) |
2 2 | Na2EDTA·2H2O | 186.12 |
3 3 | NaOH (approx. for pH 8.0) | 20 |
Preparation
- Add ~80% of the final volume of dH2O to a sterile container.
Generally, the capacity of the container should be greater than (not equal to) the final volume of solution to be prepared in order to prevent the solution from boiling over during the autoclave step.
Ensure you have a volumetric flask measuring your desired final volume.
- Adjust the final volume and molar concentration in the table above and add the calculated amounts to the dH2O.
Solution likely will not completely dissolve until at correct pH value.
- Titrate solution to desired pH using NaOH pellets.
The expected amount of NaOH to use for a pH of 8.0 can be found in the table above.
- Once completely dissolved, transfer the solution to a sterile volumetric flask and adjust the final volume using dH2O, then return it to the container.
Ensure the solution is well mixed before returning it to the container.
- Apply autoclave tape to the container and autoclave on liquids cycle.
CRITICALLiquids cycle.
- Store at room temperature.
This protocol is for the preparation of Ethylenediaminetetraacetic acid (EDTA) at various concentrations.