Use our fast CRISPR gRNA design tool to specify a gene or genome coordinates, and Benchling will automatically annotate it with the exon and CDS information you need to design guides. We support 40+ reference genomes and by request can add support for new ones within hours.

Get scores for your guides covering off-target effects and on-target efficiency within seconds. Browse your sequence while examining scores to find the best guides in important domains of a gene.

Save the guides as oligos on Benchling, organize them using tags and folders, and clone them into CRISPR plasmids. Alternatively, you can export your guides, along with scores and off-target sites, for easy use in your own spreadsheets.