Potassium Phosphate Buffer Preparation
Updated 8/6/2015 11:38 PM
Introduction
Materials
- Consumables
- Disposables
- pipettes
- Equipment
- pipettors
- volumetric flask
- media bottle
Procedure
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 1000 |
2 2 | Final Concentration (M)* | 0.5 |
Table3
A A | B B | |
1 1 | Stock Solution | Concentration (M)* |
2 2 | monobasic | 1 |
3 3 | dibasic | 1 |
Table4
A A | B B | C C | D D | |
1 1 | pH | monobasic (mL) | dibasic (mL) | dH2O (mL) |
2 2 | 5.8 | 457.5 | 42.5 | 500 |
3 3 | 6.0 | 434 | 66 | 500 |
4 4 | 6.2 | 404 | 96 | 500 |
5 5 | 6.4 | 361 | 139 | 500 |
6 6 | 6.6 | 309.5 | 190.5 | 500 |
7 7 | 6.8 | 251.5 | 248.5 | 500 |
8 8 | 7.0 | 192.5 | 307.5 | 500 |
9 9 | 7.2 | 141.5 | 358.5 | 500 |
10 10 | 7.4 | 99 | 401 | 500 |
11 11 | 7.6 | 67 | 433 | 500 |
12 12 | 7.8 | 46 | 454 | 500 |
13 13 | 8.0 | 30 | 470 | 500 |
This protocol has been adapted from Sambrook & Russell for the preparation of potassium phosphate buffer from potassium phosphate stock solutions at various pH values.
Sambrook, J. & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual, 3 edn. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. pp. A1.5