Glucose Stock Preparation
Updated 6/12/2015 07:31 PM
Introduction
Materials
- Consumables
- glucose/dextrose
- Disposables
- 0.22μm filter
- syringe (optional)
- weigh dishes/paper
- Equipment
- balance
- beaker
- volumetric flask
Procedure
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 30 |
2 2 | Final Concentration (M)* | 2 |
Table3
A A | B B | C C | |
1 1 | Component | Hydrates* | Weight (g) |
2 2 | glucose/dextrose | 0 | 10.8096 |
Preparation
- Add glucose to ~80% of the final volume of dH2O in a sterile beaker.
- Once fully dissolved, transfer the solution to a volumetric flask and carefully bring the solution to the final volume using dH2O.
- Pass the fully dissolved solution through a 0.22μm filter and into a fresh, sterile container.
- If desired, aliquot into more conveniently sized containers for storage.
- Store at 4°C for short term and -20°C for longer term.
This protocol is for obtaining a glucose stock solution at various concentrations.
Note: Glucose stock solutions typically show slight degradation upon autoclaving (indicated by color change), so sterilization through a 0.22μm filter is recommended for the most accurate concentrations.