Protocols · Benchling

Materials

Consumables
- ampicillin (sodium salt or trihydrate)
- 1N NaOH
- ethyl alcohol

Disposables
- light-tight sealed PP container
- weigh dishes/paper
Equipment
- balance

Dilution in Selective Media

Media Volume (ml)*

1000

Desired Ampicillin Media Concentration (μg/ml)*

100

Ampicillin Stock Volume (μl)

2000

Set the Media Volume, Desired Media Concentration, and Final Concentration (below) parameters to return the proper volume of antibiotic stock to add to the media.

Component Composition

Final Volume (ml)*

Final Concentration (mg/ml)*

Component	Amount	Unit
Ampicillin trihydrate	577.330356887324	mg
1N NaOH	1.5	ml
ethyl alcohol	7	ml
dH2O	to final volume

Component

Amount

Unit

Ampicillin trihydrate

577.330356887324

1N NaOH

1.5

ethyl alcohol

dH2O

to final volume

Component	Amount	Unit
Ampicillin sodium salt	531.453020806502	mg
ethyl alcohol	7	ml
dH2O	to final volume

Component

Amount

Unit

Ampicillin sodium salt

531.453020806502

ethyl alcohol

dH2O

to final volume

Preparation

Add components in the order listed above to a light-tight PP container.

CRITICALBe sure to add the NaOH to the Ampicillin trihydrate stock to solubilize, otherwise it will not fully dissolve.

Once solution is fully dissolved, store at -20°C.

There is no need to filter sterilize this solution since it is 70% ethyl alcohol.