Protocols · Benchling

Materials

Final Volume (ml)*

100

Component	Stock Conc. (M)*	Amount	Unit
Tris-Cl (pH 8.5)	1	1	ml
EDTA (pH 8.0)	0.5	0.4	ml
lysozyme (optional)		10	mg
dH2O		to final volume

Component

Stock Conc. (M)*

Amount

Unit

Tris-Cl (pH 8.5)

EDTA (pH 8.0)

0.5

0.4

lysozyme (optional)

dH2O

to final volume

If using lysozyme, use immediately; otherwise, store at room temperature for short term.

Non-lysozyme solution can be autoclaved if desired.