Preparation of 10mL of glycerol solution 50% v/v

1. Add 5mL of ddH₂0 inside a falcon tube of 50mL

2. At the same falcon tube, add 5mL of glycerol 100%

3. Mix well

4. Take in the whole glycerol solution volume with a syringe

5. Apply the syringe filter at the tip of the syringe

6. Pour slowly the glycerol solution inside a new falcon tube of 50mL

Important Note:

1. the whole procedure mentioned above, should be conducted under sterile conditions using the fire of a gas burner.

2. check if the syringe fits the falcon tube and if its tip can reach the falcon tube bottom

Glycerol stock preparation

7. For glycerol stocks, add the required volume of an overnight culture to a cryovial and the necessary glycerol volume to have a 25 % (v/v) final glycerol concentration. The final stock volume can be 1mL.