Tetracycline (Tet) Stock Preparation
Updated 6/29/2015 05:25 PM
Introduction
Materials
- Consumables
- tetracycline hydrochloride
- ethyl alcohol
- Disposables
- light-tight PP container
- weigh dishes/paper
- Equipment
- balance
Procedure
Dilution in Selective Media
Table4
A A | B B | |
1 1 | Media Volume (mL)* | 1000 |
2 2 | Desired Tetracycline Media Concentration (μg/mL)* | 10 |
3 3 | Tetracycline Volume (μL) | 2000 |
Set the Media Volume, Desired Media Concentration, and Final Concentration (below) parameters to return the proper volume of antibiotic stock to add to the media.
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 10 |
2 2 | Final Concentration (mg/mL)* | 5 |
Table3
A A | B B | C C | |
1 1 | Component | Amount | Unit |
2 2 | Tetracycline HCl | 54.1017910179 | mg |
3 3 | ethyl alcohol | 7 | mL |
4 4 | dH2O | to final volume |
Preparation
- Add components in the order listed above to a light-tight PP container.
- Once solution is fully dissolved, store at -20°C.
There is no need to filter sterilize this solution since it is 70% ethyl alcohol.
Comments
Should i dilute tetracycline in DMSO? Thank you.
This protocol describes the preparation of a Tetracycline stock solution. The concentration of this stock solution is based on the weight of anhydrous Tetracycline.
Note: Typical stock solutions for this antibiotic in selectable media are made at 5mg/mL and are used at a final
concentration of 10 μg/mL for stringent plasmids to 50 μg/mL for relaxed plasmids.
Note: The preparation in ethanol allows the solution to be stored at -20°C without degrading through successive
freeze-thaw cycles.
Note: Selective solid medium prepared with this stock solution has proven selective even after 1 year of storage.