Induction

Use this table to calculate the volume of IPTG stock required to acheive the desired final concentration in the media.

NOTE: The stock IPTG concentration is pulled from the input below.

Component Composition

Preparation

1. Add IPTG to ~80% of the final volume of dH₂O in a sterile beaker.

2. Once fully dissolved, transfer the solution to a volumetric flask and carefully bring the solution to the final volume using dH₂O.

3. Pass the fully dissolved solution through a 0.22μm filter and into a fresh, sterile container.

4. If desired, aliquot into more conveniently sized containers for storage.

5. Store at 4°C for short term and -20°C for longer term.