IPTG Stock Preparation
Updated 6/12/2015 07:42 PM
Introduction
Materials
- Consumables
- IPTG
- Disposables
- weigh dishes/paper
- 0.22 μm filter
- syringe (optional)
- Equipment
- balance
- beaker
- container
Procedure
Induction
Table4
A A | B B | |
1 1 | Media Volume (mL)* | 200 |
2 2 | IPTG Final Conc (mM)* | 0.1 |
3 3 | IPTG Stock Volume (mL) | 0.2 |
Use this table to calculate the volume of IPTG stock required to acheive the desired final concentration in the media.
NOTE: The stock IPTG concentration is pulled from the input below.
Component Composition
Table1
A A | B B | |
1 1 | Final Volume (mL)* | 10 |
2 2 | Final Concentration (mM)* | 100 |
Table3
A A | B B | |
1 1 | Component | Weight (mg) |
2 2 | IPTG | 238.3 |
Preparation
- Add IPTG to ~80% of the final volume of dH2O in a sterile beaker.
- Once fully dissolved, transfer the solution to a volumetric flask and carefully bring the solution to the final volume using dH2O.
- Pass the fully dissolved solution through a 0.22μm filter and into a fresh, sterile container.
- If desired, aliquot into more conveniently sized containers for storage.
- Store at 4°C for short term and -20°C for longer term.
This protocol describes the preparation of a Isopropyl β-D-1-thiogalactopyranoside (IPTG) stock solution at various concentrations.
A typical stock solution concentration is 100mM IPTG.
A typical final concentration when using IPTG to induce protein expression under a lac operon is 0.1mM IPTG. For direct application to a solid media plate, add 56 μL of a 100mM IPTG stock solution directly to the plate top and allow to dry before inoculating plate.