Component Composition

Preparation

1. Add ~80% of the final volume of dH₂O to a sterile media bottle.

Generally, the capacity of the bottle should be greater than (not equal to) the final volume of media to be prepared in order to prevent the media from boiling over during the autoclave step.

Ensure you have a volumetric flask measuring your desired final volume.

2. Adjust the final volume and molar concentration in the table above and add the calculated amounts of tris-base, boric acid, and EDTA (pH 8.0) to the dH₂O.

Solution likely will not completely dissolve until all components are present.

3. Once completely dissolved, transfer the solution to a sterile volumetric flask and adjust the final volume using dH₂O, then return it to the media bottle.

Ensure the solution is well mixed before returning it to the media bottle.

4. Apply autoclave tape to the bottle and autoclave on liquids cycle.

CRITICALLiquids cycle.

5. Store at room temperature.