Prepare YPD mixture as follows:

ingredients as given yield 1 liter total media (we divided it into 0.5 liter with agar and 0.5 liter liquid)

1. Balance room: appropriate Peptone and Yeast-extract-powder in glass beaker

2. Add around 800mL dH20 from grey tap in floor

3. Nanodrop/PCR room: magnetstir the beaker until powder dissolved and during this measure PH: Adjust PH to 6.5 if necessary (use 6M HCL bottle and plastic pasteurpipette) NOTE: make sure to always rinse the PHmeter before and after use properly with dH20)

4. Transfer the liquid from the beaker to 1 liter measuring cylinder, then add dH₂O up to 950mL.

5. Divide liquid in the two bottles: in one bottle add 10g agar (for pouring plates).

6. Autoclave the bottles.

7. Later when we need it: Microwave the bottle until the agar is liquid. Allow medium to cool to ~ 55°C and then add dextrose (glucose) to 2% (50mL of a sterile 40% stock solution)

8. Adjust the final volume to 1L if necessary.

9. For kanamycin-containing medium, prepare YPD as above. After autoclaved medium has cooled to 55°C, add 0.2–0.3mL of 50mg/mL kanamycin (final concentration 10–15mg/L)

40% glucose stock solution:

10. calculated for 300mL: Take 120g glucose, add dH₂O up to 300mL, magnetstir until dissolved, then autoclave.