Welcome to Benchling

Benchling is an intelligent research platform with tools for note-taking, molecular biology, and sample tracking.

Create an account

Email
Already have an account?
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
0 of 0
Advanced Settings
FabricationEQM_KDCh_20250401_ChipHeightFormulaProtocol
  • Notes
Editing disabled on read-only entry.

 
FabricationEQM_KDCh_20250401_ChipHeightFormulaProtocol

Tuesday, 4/1/2025
This entry includes fabrication details and additions in the PDMS Chip Inventory sheet.
The goal is to fabricate EQM chips with 4-4.5 mm height.
(Check out to this (and other) conversations regarding chip holder design)
PROCEDURE
Fabrication
This entry integrates protocol changes mentioned in Fabrication_GB_MS_20240826 and also FabricationEQM_KDCh_20250401_HeightFormulaProtocol and follows the inserted protocol (see above.)
Also a pastel pippete is used insted of a silicone spatula to collect the last pdms portion and therefore there was a little more loss, but no fog apperead and the chip was crystal clear.
Glass petri dishes were sprayed with 70% ethanol and cleaned with a paper towel before use.
Fabrication details are provided below:
Fabrication 1
ChipHeight Formula
In order to have the wanted chip height I did some calculations and the results are:
Wanted chip height: 4-4.5 mm
Volume of pdms: V=22 ml
Covered area of the wafer's diametre: D=7.89-8.37cm
Therefore the area covered with the alouminioum foil at the edges of the wafer at every side shoundn't be more that 1.05 cm and less that 0.81 cm.
486495133_852124950402514_7768953618592018412_n.jpg
Loading...
Loading...
IMPORTANT NOTES
It's seems that the results from the formula were not accurate, because there is about 0,7-0,9g loss of pdms in order to avoid the appearance of fog. This also happened at the last 2-3 fabrications and therefore from now on I'll use V=21ml (g) instead of V= 22 ml (g)
So it's:
For chip height 3mm: D=9,44cm, g=0.56cm g'=0.28mm
For chip height 3,5mm: D=8,74cm, g=1.26cm g'=0.63cm
For chip height 4mm: D=8,17cm g=1,82cm g'=
0,91cm
For chip height 4,5mm: D=7.7cm, g=2.3cm g'=1.15cm
For chip height 5mm: D=7.31cm, g=2.69cm g'=1.35cm
Also after puring the pdms there is a significant portion of it under the alouminioum foil at the edges and therefore its very important to push down the alouminioum foil again.
OUTCOMES
Chips
Chip1 #160 (1cm gap) came out with almost consistent chip height of 4-3.60mm and 3.85 mm
Chip2 #161 (0.8cm gap) came out with quit unconsistent chip height of 3.97-3.66. 3.95. 3.70mm
Chip3 #162 (1-0.8cm gap ) came out in good condition with consistent chip height of 3.85mm

Welcome to Benchling!

Sign in to view data.

Split Workspace