Welcome to Benchling

Benchling is an intelligent research platform with tools for note-taking, molecular biology, and sample tracking.

Create an account

Email
Already have an account?
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
0 of 0
Advanced Settings
Fabrication_GB_MS_20240826_ProtocolDifferences
  • Notes
Editing disabled on read-only entry.

 
Fabrication_GB_MS_20240826_ProtocolDifferences

Monday, 8/26/2024
INTRODUCTION
Assuring the current protocol works, the following chips are fabricated to increase height to 4-5mm vs the previous achieved height of 2-3mm, based on 2 factors;
Steady PDMS total volume
Losses during pouring
PROCEDURE
Differences introduced (vs protocol)
Aluminum foil was NOT used for the petri dishes, as a well cleaned, glass dish was sufficient for the procedure and decreased the losses of PDMS volume
A thin, bendable silicone spatula was used to pour the PDMS into the wafer and minimized losses almost to 0%. Pictures depict the used spatula, which has been stored with the glass petri dishes used for fabrication. (Soon to be added in the Inventory doc.)
1724744324848.jpg
Loading...
Loading...
1724744324831.jpg
Loading...
Loading...
The following pictures show the technique that was used to cover the wafer with aluminium foil to reduce radius by ~1 cm and ensure no leakage.
20240826_143944.jpg
Loading...
Loading...
20240826_143952.jpg
Loading...
Loading...
Preparation
Clean the glass petri dishes using ethanol or acetone
Fabrication
Time in the desiccator: 45'
Baking time: 30'
Time in the desiccator: 30'
Baking time: 30'
OUTCOMES
Chips #29 - 36 (DM) came out in good condition with a height of 4mm (#29 - 30) and 4.5mm (#31 - 36)
Chip #28 didn't make it through, it was ruptured during cutting.
Chips #37 - 43 (EQM) came out in good condition with consistent chip height / wafer equaling to 4.5mm (#37 - 39) and 5mm (#40 - 43).
They also needed less time at the desiccator

Welcome to Benchling!

Sign in to view data.

Split Workspace