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keio cassette plasmid
  • Sequence Map
Editing is disabled because sequence edits have been locked.
tgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctgGAATTCCTGCTGCGGAGATCTGCTGCTAGCGTGACGTAGGGAAGTTCCTATTCTCTAacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagacCTTAAGGACGACGCCTCTAGACGACGATCGCACTGCATCCCTTCAAGGATAAGAGATBmtIFokINheIXbaIZraIAatIIBglIIBsaHIBssSIBtsCIEcoRIBssSαI3k3 Pre-prefixstem loo...minatorbglBrk PrefixpGA pre...Brick)Vf2 (Bi...rimer)Vf2 (bi...rimer)H1FRT20406080100120140160
GAAAGTATAGGAACTTCttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattcCTTTCATATCCTTGAAGaataatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtttcttaccgttccaggaccattgccagacgctaagBarIAvaIIPflMIPpuMISau96IEcoO109IkanrFRT180200220240260280300320340
cgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcgctgggcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttctcgaacacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgAseIkanr360380400420440460480500520
atcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgtttagttggtttggcaataagtacgcactaacgcggactcgttctgctttatgtgctagcgacaattttcctgttaatgtttgtccttagcttacattggccgcgtccttgtgccggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttccgacaaAjuIEaeIPvuISspIBsrFIEcoNIkanr540560580600620640660680700
ttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggctaagggtccttagcgccaccactcattggtgcgtagtagtcctcatgcctattttacgaactaccagccctctccgtatttgaggcagtcggtcaactctgcctggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaAvaIIBsmBIEsp3ISau96Ikanr720740760780800820840860
tcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagtttagggtatgttcgctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgcaggtacaacctcaaattcgcgcctgccctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaNruIBanIIkanr8809009209409609801,0001,0201,040
tattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGGCAATCACCTACTCACGCGGATCCAACAGGGataacaagtactactatataaaaatagaacacgttacattgtagtctctaaaactctgtgttgcaccgaaacaacttatttagcttgaaaacgactcaacttcctagtcCTTCAAGGATAAGAGATCTTTCATATCCTTGAAGCCGTTAGTGGATGAGTGCGCCTAGGTTGTCCCBarIXbaIBamHIBsrDIDraIIIkanrFRTH2bgl...ixpGA...k)1,0601,0801,1001,1201,1401,1601,1801,2001,220
TTCTCGAGtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacAAGAGCTCaggccgtttttttgcccgttccacagtggtgggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgBsrBIE. coli his terminatorpSB3K3 Post-SuffixVr (Bio...rimer)Vr (bio...rimer)1,2401,2601,2801,3001,3201,3401,3601,3801,400
agaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatactcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatgDrdIEciINspIPciIAflIIIHpy99Irep pMB11,4201,4401,4601,4801,5001,5201,5401,560
caggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacgtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttgEciISfcIApaLIBaeGIBciVIBseYIBssSIHaeIIBssSαIBsiHKAIrep pMB11,5801,6001,6201,6401,6601,6801,7001,7201,740
cccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactaggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcSfcIAlwNIrep pMB11,7601,7801,8001,8201,8401,8601,8801,9001,920
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgcAcuIrep pMB11,9401,9601,9802,0002,0202,0402,0602,0802,100
gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaacccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcgagctccgaacctaagagtggttatttttPspXI2,1202,1402,1602,1802,2002,2202,2402,260
acgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaatgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttBsmIFauISacIScaITatIBanIIBtgZIEcoRVBsiHKAIEco53kIChlorR2,2802,3002,3202,3402,3602,3802,4002,4202,440
cggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacccgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggBtgIEaeIMscINcoIPasISspIStyIBsmBIEsp3IPflMIChlorR2,4602,4802,5002,5202,5402,5602,5802,6002,620
tttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaAclIBpmIBtgZIHpy99IChlorR2,6402,6602,6802,7002,7202,7402,7602,7802,800
ttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaaBsmIFauIFokIBspEIBsrDIBtsCIPvuIIChlorR2,8202,8402,8602,8802,9002,9202,9402,960
ctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgaggaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcMslIBaeGIBsaAIPspXIChlorR2,9803,0003,0203,0403,0603,0803,1003,1203,140
  • Plasmid
Editing is disabled because sequence edits have been locked.
3144 bp2250250027503000250500750kanrChlorRpGA prefix (bglBrick)

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3144