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    8/4: Overnights for Minipreps and Transforming GFP
    • Notes
    • Metadata
    • Relevant Items
    • Miniprep Plasmid DNA Purification from E. Coli
    • iGEM Distribution Kit Plate Instruction
    • TSS Chemical Transformation
    Editing disabled on read-only entry.

     
    8/4: Overnights for Minipreps and Transforming GFP

    Sunday, 8/4/2019ObjectiveTransformationTable1OvernightsTable2
    Sunday, 8/4/2019
    Objective
    Make Minipreps of Double Terminator, FixK2 promoter, and sfGFP+dCBD.
    Also transformations (right below this)
    Transformation
    Transform GFP: 2019 dist kit plate 6 well 13K
    Resuspension of GFP 1:45
    Icing: 1:59
    Incubation: 3:09
    Making Amp Plates:
    Add 1uL of 1000X anitbiotic to 1mL media
    Overnights

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