Benchling failed to load. Try refreshing the page. If the issue persists,
contact
support@benchling.com for help. Benchling works best when using a
supported browser.
Benchling is actively tested against the latest versions of Chrome,
Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** -
for more information, [click here](/browsers).
Monospace normal
Monospace bold
loading...
working...
0 of 0
Advanced Settings
8/9: Transformation of ho1 and pcyA and Miniprep of GFP
loading...
Notes
Metadata
Relevant Items
Miniprep Plasmid DNA Purification from E. Coli
TSS Chemical Transformation
Editing disabled on read-only entry.
8/9: Transformation of ho1 and pcyA and Miniprep of GFP
This section should list any notes of changes in the Protocols and other notes of interest.
Miniprep
●
I screwed up and didn't do the second centrifuge step to remove all the wash buffer. I threw the samples out. We will have to make overnights tomorrow. The successful transformations have been put into the fridge.
Transformation
Conclusion
Just some stuff we needed to do. I also poured nine more chlor plates. We also need more LB agar as well and SOC media should be prepared.
Cut sites of enzymes that you select are highlighted to help guide your work.
(Enzymes with compatible ends turn the same color.)
Selecting cut sites and copying the sequence will also activate enzymes.
See the "Cloning by restriction enzyme digest" tutorial under Sequence Construction in Help for more information.