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8/9: Transformation of ho1 and pcyA and Miniprep of GFP
  • Notes
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8/9: Transformation of ho1 and pcyA and Miniprep of GFP

Friday, 8/9/2019
Objectives
Miniprep GFP
Significant Notes
This section should list any notes of changes in the Protocols and other notes of interest.
Miniprep
I screwed up and didn't do the second centrifuge step to remove all the wash buffer. I threw the samples out. We will have to make overnights tomorrow. The successful transformations have been put into the fridge.
Transformation
Conclusion
Just some stuff we needed to do. I also poured nine more chlor plates. We also need more LB agar as well and SOC media should be prepared.

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