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pflp2
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    tgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgggaaagcagagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcPfoIVf2 (Bi...rimer)20406080100120140160
    tcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatacctctcaggcgccgctggtgccgctggttggacgccaagggtgaatccgcctcgataccctgattactcgcttcctgcgccctctcaggcggagtcccgcgcagtcgcccacaaccgcccacagccccgaccgaattgatacgccgtagtctcgtctaacatgactctcacgtggtatggagagtccgcggcgaccacggcgaccaacctgcggttcccacttaggcggagctatgggactaatgagcgaaggacgcgggagagtccgccKasINarISfoIPluTIregion containing broad-host-range origin of repli180200220240260280300320340
    cgataggggactggtaaaacggggattgcccagacgcctcccccgccccttcaggggcacaaatgcggccccaacggggccacgtagtggtgcgttttttgcgtttccacccttttcttccttttcccttttaaaccttttaggacgtctacaggccacgtaatccgtggcctgtagagctatcccctgaccattttgcccctaacgggtctgcggagggggcggggaagtccccgtgtttacgccggggttgccccggtgcatcaccacgcaaaaaacgcaaaggtgggaaaagaaggaaaagggaaaatttggaaaatcctgcagatgtccggtgcattaggcaccggacatctPflMIDraIIIregion containing broad-host-range origin of repli360380400420440460480500520
    gtttaaaaagggacggatttgttgccattaagggacggatttgttgttaagaagggacggatttgttgttgtaaagggacggatttgttgtattgtgggacgcagatacagtgtccccttatacacaaggaatgtcgaacgtggcctcacccccaatggtttacaaaagcaatgccctcaaatttttccctgcctaaacaacggtaattccctgcctaaacaacaattcttccctgcctaaacaacaacatttccctgcctaaacaacataacaccctgcgtctatgtcacaggggaatatgtgttccttacagcttgcaccggagtgggggttaccaaatgttttcgttacgggaregion containing broad-host-range origin of replirepreplication protein540560580600620640660680700
    ggtcgaggccgcgtatcgcctcagtgttcaggaacagcggatcgttctggcctgtattagccaggtgaagaggagcgagcctgtcaccgatgaagtgatgtattcagtgacggcggaggacatagcgacgatggcgggtgtccctatcgaatcttcctacaaccagctcaaagaagcgccagctccggcgcatagcggagtcacaagtccttgtcgcctagcaagaccggacataatcggtccacttctcctcgctcggacagtggctacttcactacataagtcactgccgcctcctgtatcgctgctaccgcccacagggatagcttagaaggatgttggtcgagtttcttcgcBseRIEcoNIrepreplication protein720740760780800820840860880
    gccctgcgcctgaaacggcgggaagtccggttaacccaagagcccaatggcaaggggaaaagaccgagtgtgatgattaccggctgggtgcaaacaatcatctaccgggagggtgagggccgtgtagaactcaggttcaccaaagacatgctgccgtacctgacggaactcaccaaaccgggacgcggactttgccgcccttcaggccaattgggttctcgggttaccgttccccttttctggctcacactactaatggccgacccacgtttgttagtagatggccctcccactcccggcacatcttgagtccaagtggtttctgtacgacggcatggactgccttgagtggtttgBanIIrepreplication protein9009209409609801,0001,0201,0401,060
    agttcaccaaatacgccttggctgacgtggccaagatggacagcacccacgcgatcaggctttacgagctgctcatgcaatgggacagcatcggccagcgcgaaatagaaattgaccagctgcgaaagtggtttcaactggaaggccggtatccctcgatcaaggacttcaagttgcgtcaagtggtttatgcggaaccgactgcaccggttctacctgtcgtgggtgcgctagtccgaaatgctcgacgagtacgttaccctgtcgtagccggtcgcgctttatctttaactggtcgacgctttcaccaaagttgaccttccggccatagggagctagttcctgaagttcaacgcrepreplication protein1,0801,1001,1201,1401,1601,1801,2001,2201,240
    agtgcttgatccagccgtgacgcagatcaacgagcacagcccgctacaggtggagtgggcgcagcgaaagaccgggcgcaaggtcacacatctgttgttcagttttggaccgaagaagcccgccaaggcggtgggtaaggccccagcgaagcgcaaggccgggaagatttcagatgcttcacgaactaggtcggcactgcgtctagttgctcgtgtcgggcgatgtccacctcacccgcgtcgctttctggcccgcgttccagtgtgtagacaacaagtcaaaacctggcttcttcgggcggttccgccacccattccggggtcgcttcgcgttccggcccttctaaagtctacgaBstXIrepreplication protein1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gagatcgcgaaacaggctcgccctggtgagacatgggaagcggcccgcgctcgactaacccagatgccgctggatctggcctagaggccgtggccaccacggcccggcctgcctttcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggctctagcgctttgtccgagcgggaccactctgtacccttcgccgggcgcgagctgattgggtctacggcgacctagaccggatctccggcaccggtggtgccgggccggacggaaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccNruIPvuISfiIrepreplication protein1,4401,4601,4801,5001,5201,5401,5601,5801,600
    cgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcagttcacttacaccgcttctcaacccggtacgcaccagaaaatcattgatatggccatgaatggcgttggatgccggctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacggttcgaacgtacggacgtcaagtgaatgtggcgaagagttgggccatgcgtggtcttttagtaactataccggtacttaccgcaacctacggcBmrIPstISphIM13-fwd1,6201,6401,6601,6801,7001,7201,7401,7601,780
    ggcaacagcccgcattatgggcgttggcctcaacacgattttacgtcacttaaaaaactcaggccgcagtcggtaacctcgcgcatacagccgggcagtgacgtcatcgtctgcgcggaaatggacgaacagtggggctatgtcggggctaaatcgcgccagcgctggctgttttacgccgttgtcgggcgtaatacccgcaaccggagttgtgctaaaatgcagtgaattttttgagtccggcgtcagccattggagcgcgtatgtcggcccgtcactgcagtagcagacgcgcctttacctgcttgtcaccccgatacagccccgatttagcgcggtcgcgaccgacaaaatgcMluIAflIIIBstEII1,8001,8201,8401,8601,8801,9001,9201,940
    cgtatgacagtctccggaagacggttgttgcgcacgtattcggtgaacgcactatggcgacgctggggcgtcttatgagcctgctgtcaccctttgacgtggtgatatggatgacggatggctggccgctgtatgaatcccgcctgaagggaaagctgcacgtaatcagcaagcgatagcatactgtcagaggccttctgccaacaacgcgtgcataagccacttgcgtgataccgctgcgaccccgcagaatactcggacgacagtgggaaactgcaccactatacctactgcctaccgaccggcgacatacttagggcggacttccctttcgacgtgcattagtcgttcgctatBbsIBsgIBspEI1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tacgcagcgaattgagcggcataacctgaatctgaggcagcacctggcacggctgggacggaagtcgctgtcgttctcaaaatcggtggagctgcatgacaaagtcatcgggcattatctgaacataaaacactatcaataagttggagtcattaccaaaaggttaggaatacggttaatgcgtcgcttaactcgccgtattggacttagactccgtcgtggaccgtgccgaccctgccttcagcgacagcaagagttttagccacctcgacgtactgtttcagtagcccgtaatagacttgtattttgtgatagttattcaacctcagtaatggttttccaatccttatgccaatCspCIPflFIPshAITth111I2,1402,1602,1802,2002,2202,2402,2602,2802,300
    gccatttgcctgcttttatatagttcatatgggattcacctttatgttgataagaaataaaagaaaatgccaataggatatcggcattttcttttgcgtttttatttgttaactgttaattgtccttgttcaaggatgctgtctttgacaacagatgttttcttgcctttgatgttcacggtaaacggacgaaaatatatcaagtataccctaagtggaaatacaactattctttattttcttttacggttatcctatagccgtaaaagaaaacgcaaaaataaacaattgacaattaacaggaacaagttcctacgacagaaactgttgtctacaaaagaacggaaactacaagtNdeIEcoRVlevansucrasesacB2,3202,3402,3602,3802,4002,4202,4402,4602,480
    gcaggaagctaggcgcaaacgttgattgtttgtctgcgtagaatcctctgtttgtcatatagcttgtaatcacgacattgtttcctttcgcttgaggtacagcgaagtgtgagtaagtaaaggttacatcgttaggatcaagatccatttttaacacaaggccagttttgttcagcggcgtccttcgatccgcgtttgcaactaacaaacagacgcatcttaggagacaaacagtatatcgaacattagtgctgtaacaaaggaaagcgaactccatgtcgcttcacactcattcatttccaatgtagcaatcctagttctaggtaaaaattgtgttccggtcaaaacaagtcgcclevansucrasesacB2,5002,5202,5402,5602,5802,6002,6202,6402,660
    cttgtatgggccagttaaagaattagaaacataaccaagcatgtaaatatcgttagacgtaatgccgtcaatcgtcatttttgatccgcgggagtcagtgaacagataccatttgccgttcattttaaagacgttcgcgcgttcaatttcatctgttactgtgttagatgcaatcagcgaacatacccggtcaatttcttaatctttgtattggttcgtacatttatagcaatctgcattacggcagttagcagtaaaaactaggcgccctcagtcacttgtctatggtaaacggcaagtaaaatttctgcaagcgcgcaagttaaagtagacaatgacacaatctacgttagtcgSacIIlevansucrasesacB2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ggtttcatcacttttttcagtgtgtaatcatcgtttagctcaatcataccgagagcgccgtttgctaactcagccgtgcgttttttatcgctttgcagaagtttttgactttcttgacggaagaatgatgtgcttttgccatagtatgctttgttaaataaagattcttcgccttggtccaaagtagtgaaaaaagtcacacattagtagcaaatcgagttagtatggctctcgcggcaaacgattgagtcggcacgcaaaaaatagcgaaacgtcttcaaaaactgaaagaactgccttcttactacacgaaaacggtatcatacgaaacaatttatttctaagaagcggaaccalevansucrasesacB2,8602,8802,9002,9202,9402,9602,9803,0003,020
    agccatcttcagttccagtgtttgcttcaaatactaagtatttgtggcctttatcttctacgtagtgaggatctctcagcgtatggttgtcgcctgagctgtagttgccttcatcgatgaactgctgtacattttgatacgtttttccgtcaccgtcaaagattgatttataatcctctcggtagaagtcaaggtcacaaacgaagtttatgattcataaacaccggaaatagaagatgcatcactcctagagagtcgcataccaacagcggactcgacatcaacggaagtagctacttgacgacatgtaaaactatgcaaaaaggcagtggcagtttctaactaaatattaggagBplIBplIPsiIBsrGISnaBIlevansucrasesacB3,0403,0603,0803,1003,1203,1403,1603,1803,200
    tacaccgttgatgttcaaagagctgtctgatgctgatacgttaacttgtgcagttgtcagtgtttgtttgccgtaatgtttaccggagaaatcagtgtagaataaacggatttttccgtcagatgtaaatgtggctgaacctgaccattcttgtgtttggtcttttaggatagaatcaatgtggcaactacaagtttctcgacagactacgactatgcaattgaacacgtcaacagtcacaaacaaacggcattacaaatggcctctttagtcacatcttatttgcctaaaaaggcagtctacatttacaccgacttggactggtaagaacacaaaccagaaaatcctatcttagtBsgIlevansucrasesacB3,2203,2403,2603,2803,3003,3203,3403,3603,380
    tttgcatcgaatttgtcgctgtctttaaagacgcggccagcgtttttccagctgtcaatagaagtttcgccgactttttgatagaacatgtaaatcgatgtgtcatccgcatttttaggatctccggctaatgcaaagacgatgtggtagccgtgatagtttgcgacagtgccgtcagaaacgtagcttaaacagcgacagaaatttctgcgccggtcgcaaaaaggtcgacagttatcttcaaagcggctgaaaaactatcttgtacatttagctacacagtaggcgtaaaaatcctagaggccgattacgtttctgctacaccatcggcactatcaaacgctgtcacggcagtcPciIAflIIIlevansucrasesacB3,4003,4203,4403,4603,4803,5003,5203,5403,560
    cgttttgtaatggccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggcaaaacattaccggtcgacagggtttgcaggtccggaaaacgtcttctctataaaaattaacacctgcttagcttgagtccttgaactataaaaagtaaaaaaacgacaagtccctaaacgtcgtatagtaccgcacattataccctttacggcatacaaaggaatataccgaaaacStuIlevansucrasesacB3,5803,6003,6203,6403,6603,6803,7003,720
    gttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttcaagcaaagaaagcgtttgcgaactcaacgcggaggacggtcgtcacgccatcatttccaattatgacaacgaacaaaacgtttgaaaaactacaagtagcaagtacagaggaaaaaatacatgacacaatcgccagacgaagaaggtcgggaggacaaacttctaccgttcaatcaalevansucrasesacB3,7403,7603,7803,8003,8203,8403,8603,8803,900
    acgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgcgtgttatttttttctggattttatacattccccactgcggtttcatatgtgaaacgggaaatgtgtaaaatccagaacggacgaaatagtcattgtttgggcgcgctaaatgaaaagctggagtaagataatctgagagcaaacctaacgttgaccagataaaaggagaaaacaaRNAp binding m...(17bp spacer)3,9203,9403,9603,9804,0004,0204,0404,0604,080
    tgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcactatcttttagtattttcctaaacgtctgatgcccggatttcttgattttttagatagacaaagaaaagtaagagacataaaaaatatcaaagacaacgtacccgtatttcaacggaaaaattagtgttaagtcttttatagtattatagagtaaagtgatttattatcacttgccg4,1004,1204,1404,1604,1804,2004,2204,2404,260
    aggtatatgtgatgggttaaaaaggatcgatcctctagaggatccccctagagaataggaacttcggaataggaacttcaaagcgtttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctcactgttcacgtcgcacctatatctgcgtgttgcctgtattccatatacactacccaatttttcctagctaggagatctcctagggggatctcttatccttgaagccttatccttgaagtttcgcaaaggcttttgctcgcgaaggcttttacgttgcgctcgacgcgtgtatgtcgagtgacaagtgcagcgtggatatagacgcacaacggacataXbaIBamHI4,2804,3004,3204,3404,3604,3804,4004,4204,440
    atatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaaggtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgcttccttcagcactaccctttagctgttctatatgctgccacttatatatatgtactcttcttgccgtatcacgcacaaatacgaatttacgcatgaatatacgcagataaatacatcctactttccatcagatcatggaggacactataatagggtaaggtacgccccatagcatacgaaggaagtcgtgatgggaaatcgacaagatatacgacggtgaBarIBseRIFlp recombinaseflp4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcatatgcatagtaccgagaaactagtgcgaagtagtgatcaggtattgctgttatctgatgagtatacgttgtcctggccacggcagaagcacgcttatcgctccaatttcccacaacattagtggagttaacctaatcagagtaggaagttacgatagtaaaggaaactataacctagtatacgtatcatggctctttgatcacgcttcatcactagtccataacgacaatagactactcatatgcaacaggaccggtgccgtcttcgtgcgaatagcgaggttaaagggtgttgtaatcaBcgIBclINdeINsiISpeIFlp recombinaseflp4,6404,6604,6804,7004,7204,7404,7604,7804,800
    caactccgttaggcccttcattgaaagaaatgaggtcatcaaatgtcttccaatgtgagattttgggccattttttatagcaaagattgaataaggcgcatttttcttcaaagctttattgtacgatctgactaagttatcttttaataattggtattcctgtttattgcttgaagaagttgaggcaatccgggaagtaactttctttactccagtagtttacagaaggttacactctaaaacccggtaaaaaatatcgtttctaacttattccgcgtaaaaagaagtttcgaaataacatgctagactgattcaatagaaaattattaaccataaggacaaataacgaacttcttBbsIFlp recombinaseflp4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ttgccggtcctatttactcgttttaggactggttcagaattcctcaaaaattcatccaaatatacaagtggatcgatcctaccccttgcgctaaagaagtatatgtgcctactaacgcttgtctttgtctctgtcactaaacactggattattactcccagatacttattttggactaaacggccaggataaatgagcaaaatcctgaccaagtcttaaggagtttttaagtaggtttatatgttcacctagctaggatggggaacgcgatttcttcatatacacggatgattgcgaacagaaacagagacagtgatttgtgacctaataatgagggtctatgaataaaacctgatEcoRIFlp recombinaseflp5,0005,0205,0405,0605,0805,1005,1205,1405,160
    atttaaatgatttcggatcaacgttcttaatatcgctgaatcttccacaattgatgaaagtagctaggaagaggaattggtataaagtttttgtttttgtaaatctcgaagtatactcaaacgaatttagtattttctcagtgatctcccagatgctttcaccctcacttagaagtgctaaatttactaaagcctagttgcaagaattatagcgacttagaaggtgttaactactttcatcgatccttctccttaaccatatttcaaaaacaaaaacatttagagcttcatatgagtttgcttaaatcataaaagagtcactagagggtctacgaaagtgggagtgaatcttcacgSwaIFlp recombinaseflp5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tttaagcatttttttactgtggctatttcccttatctgcttcttccgatgattcgaactgtaattgcaaactacttacaatatcagtgatatcagattgatgtttttgtccatagtaaggaataattgtaaattcccaagcaggaatcaatttctttaatgaggcttccagaattgttaaattcgtaaaaaaatgacaccgataaagggaatagacgaagaaggctactaagcttgacattaacgtttgatgaatgttatagtcactatagtctaactacaaaaacaggtatcattccttattaacatttaagggttcgtccttagttaaagaaattactccgaaggtcttaacaaBstBIEcoRVFlp recombinaseflp5,3605,3805,4005,4205,4405,4605,4805,500
    gctttttgcgtcttgtatttaaactggagtgatttattgacaatatcgaaactcagcgaattgcttatgatagtattatagctcatgaatgtggctctcttgattgctgttccgttatgtgtaatcatccaacataaataggttagttcagcagcacataatgctattttctcacctgcgaaaaacgcagaacataaatttgacctcactaaataactgttatagctttgagtcgcttaacgaatactatcataatatcgagtacttacaccgagagaactaacgacaaggcaatacacattagtaggttgtatttatccaatcaagtcgtcgtgtattacgataaaagagtggacBpmIBstAPIFlp recombinaseflp5,5205,5405,5605,5805,6005,6205,6405,6605,680
    aaggtctttcaaacctttccacaaactgacgaacaagcaccttaggtggtgttttacataatataccaaattgtggcatacaacctccttagtacatgcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgttccagaaagtttggaaaggtgtttgactgcttgttcgtggaatccaccacaaaatgtattatatggtttaacaccgtatgttggaggaatcatgtacgttggtaatagtggcggtctccattttatcagttgtgcgtgccacaatctataaatagggaacgccactatctaaattgcPflMIBsu36IFlp recombinaseflpRNAp binding m...(17bp spacer)5,7005,7205,7405,7605,7805,8005,8205,8405,860
    tatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatatactcgtgttttttctttggtaattgtgttctcgtcgaactcctgcgtgcagcggaatttcgttaaatactttttttctttttacttgaaccgaatagggtccttagacagcgtctgttctacccctaccccgtcagtccgcaaccacgaaataaattaccgtagttacgtaatttaNsiIPshAIBstAPIcIlambda repressor5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagccgaatattgcggcgtaacgaatgtttttaagagtttcaatcgcaacttcttaaatcgggaagttagcggtctctttagatgctctacatacttcgccaatcatacgtcggcagtgaatcttcactcatactcatgggacaaaaaagagtacaagtccgtccctacaagagtggattcgPsiIcIlambda repressor6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggaatcttggaaatggtttccactacgcctctctacccattcgtgttggttttttcggtcactaagacgtaagaccgaactccaacttccattaaggtactggcgtggttgtccgaggttcggttcgaaaggactgccttacaattaagagcaactgggactcgtccgacaactcggtccBsmIcIlambda repressoraraC ...ator6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcactaaagacgtatcggtctgaacccccactactcaaatggaagttctttgactagtccctatcgccagtccacaaaaatgttggtgatttgggtgtcatgggttactagggtacgttactctcaacaaggcaacacccctttcaatagcgatcagtcaccggacttctctgcaaaccgAjuIBclIcIlambda repressor6,4206,4406,4606,4806,5006,5206,5406,5606,580
    tgatcggcaaggtgttctggtcggcgcatagctgataacaattgagcaagaatcttcatcgaattaggggaattttcactcccctcagaacataacatagtaaatggattgaattatgaagaatggtttttatgcgacttaccgcagcaaaaataaagggaaagataagcgctcaataactagccgttccacaagaccagccgcgtatcgactattgttaactcgttcttagaagtagcttaatccccttaaaagtgaggggagtcttgtattgtatcatttacctaacttaatacttcttaccaaaaatacgctgaatggcgtcgtttttatttccctttctattcgcgagttat6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aacctgtctgttttccttaattctctgctggctgataatcatcacctgcaggttggctccaattatttgtatattcataaaatcggggtaccgagctcgaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaattggacagacaaaaggaattaagagacgaccgactattagtagtggacgtccaaccgaggttaataaacatataagtattttagccccatggctcgagcttaagcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttKpnIPstISacISbfIBanIIEcoRIPaqCIAcc65IEco53kIR0010 (pLac wild type)LacOM13-revLacO16,7806,8006,8206,8406,8606,8806,9006,9206,940
    gcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactSapIBsaXIBspQIR0010 (pLac wild type)upstream lacI homology6,9606,9807,0007,0207,0407,0607,0807,1007,120
    ctcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatggggattccttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccagagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacccctaaggaattccatatgaaaggcgacgtattgggacgaagccccagtaatatcgctaaaaaagccatataggtAflIIregion containing oriTVr (Bio...rimer)7,1407,1607,1807,2007,2207,2407,2607,280
    tcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgagaggaaaaagcgtgctatatgtcctaaaacggtttcccaagcacatctgaaaggaaccacataggttgccgcagtcggcccgtcctatccacttcatccgggtgggcgctcgcccacaaggaagaagtgacagggaataagcgtggaccgccacgagttgcccttaggacgagacgctcregion containing oriT7,3007,3207,3407,3607,3807,4007,4207,4407,460
    gctggccgataagctagcttatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccgaccggctattcgatcgaatacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaaggBmtINheIColE1 originre...T7,4807,5007,5207,5407,5607,5807,6007,6207,640
    ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggColE1 origin7,6607,6807,7007,7207,7407,7607,7807,8007,820
    gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtactggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatColE1 origin7,8407,8607,8807,9007,9207,9407,9607,9808,000
    tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaaagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttColE1 origin8,0208,0408,0608,0808,1008,1208,1408,1608,180
    cgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgtgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcabeta lactamaseblaColE1 origin8,2008,2208,2408,2608,2808,3008,3208,3408,360
    tcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcAhdIBmrIBpmIBsaINmeAIIIbeta lactamasebla8,3808,4008,4208,4408,4608,4808,5008,5208,540
    cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaabeta lactamasebla8,5608,5808,6008,6208,6408,6608,6808,7008,720
    gtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacBcgIPvuIScaIbeta lactamasebla8,7408,7608,7808,8008,8208,8408,8608,8808,900
    cggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtaXmnIbeta lactamasebla8,9208,9408,9608,9809,0009,0209,0409,060
    cttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatcttSspIbeta lactamasebla9,0809,1009,1209,1409,1609,1809,2009,2209,240
    aaataaacaaataggggttccgcgcacatttccccgaaaagtttatttgtttatccccaaggcgcgtgtaaaggggcttttc9,2609,280
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    9297 bp650070007500800085009000500100015002000M13-fwdVf2 (BioBrick primer),region containing broad-host-range origin of repli,PfoI,+6EcoNI,BseRIBanIIrep,replication proteinBstXI,NruISfiI,PvuI,BmrISphI,PstI,BstEIIAflIII,MluI,BspEI,BbsIBsgIPshAI,CspCI,PflFI,Tth111IR0010 (pLac wild type),upstream lacI homology,BsaXI,BspQI,SapIVr (BioBrick primer),AflIIregion containing oriTNheIBmtIColE1 originAhdI,BmrIbeta lactamase,bla,BsaI,BpmI,NmeAIII,PvuIScaI,BcgI,XmnI,SspI

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    9297