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L1-1 | pNOS - P19 - tOCS
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    gccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcapUC\ori20406080100120140160
    taagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcBspHIAP\rpUC\ori180200220240260280300320340
    ttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaaccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccataacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctcttggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaAhdIAP\r360380400420440460480500520
    ccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgAseIFspIAP\r540560580600620640660680700
    aaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgPvuIScaIAP\r720740760780800820840860
    ggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgXmnIBsaHIBssSIBssSαIAP\r8809009209409609801,0001,0201,040
    atcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaBspHIAP\r1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tagaaaaataaacaaataggggttccgcgcacgaattggccagcgctgccatttttggggtgaggccgttcgcggccgaggggcgcagcccctggggggatgggaggcccgcgttagcgggccgggagggttcgagaagggggggcaccccccttcggcgtgcgcggtcacgcgcatctttttatttgtttatccccaaggcgcgtgcttaaccggtcgcgacggtaaaaaccccactccggcaagcgccggctccccgcgtcggggacccccctaccctccgggcgcaatcgcccggccctcccaagctcttcccccccgtggggggaagccgcacgcgccagtgcgcgAfeIMscIPasIoriV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    acagggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctcatctgtcagcactctgtgtcccgcgtcgggaccaatttttgttccaaatatttataaccaaattttcgtccaattttctgtccaatcgccaccggctttttgcccgcctttgggaacgtttacgacctaaaagacggacacctgtcggggagtttacagttatccacgcggggagtagacagtcgtgagacPsiIoriV1,4201,4401,4601,4801,5001,5201,5401,560
    cccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggccagctccacgtcgccggccgaaatggggagttcacagttcctagcgcggggagtagacagtcatcagcgcggggagttcacagttatggcgtcccgtgaataggggtccgaacaggtgtagtagacaccctttgagcgcattttagtccgcaaaagcggctaaacgctccgaccggtcgaggtgcagcggccggctttaMscIXcmIoriV1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggcccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggccgcggtgtctcgcacacggcttcgacggcgtttctggcgcgtttgcagggccatgctcggacggggagtagacagttgcggcgcggcccactcagccggggagttcacagttgcaggcggggagtagacagtcactcccggttcaaaaggcgctccataggtgttgcggccgccggcgccacagagcgtgtgccgaagctgccgcaaagaccgcgcaaacgtcccggtaNotISacIIoriV1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agacggccgccagcccagcggcgagggcaaccagcccggtgagcgtcgcaaaggagatcctgatctgactgatgggctgcctgtatcgagtggtgattttgtgccgagctgccggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgctttctgccggcggtcgggtcgccgctcccgttggtcgggccactcgcagcgtttcctctaggactagactgactacccgacggacatagctcaccactaaaacacggctcgacggccagcccctcgacaaccgaccgaccaccgtcctatataacaccacatttgtttaactgcgaaBsaBITDNA-LBoriV1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agacaacttaataacacattgcggacgtttttaatgtactggggtggatgcagtgggccccactctgtgaagacaatgccgaattcggatccggagagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaatctgttgaattattgtgtaacgcctgcaaaaattacatgaccccacctacgtcacccggggtgagacacttctgttacggcttaagcctaggcctctcgcctcttaattccctcagtgcaatactgggggcggctactgcgccctgttcggcaaaatgcaaaccttgactgtcttApaIBbsIBamHIBanIIBspEIEcoRIDraIIIPspOMITDNA-LB2,1202,1402,1602,1802,2002,2202,2402,260
    ccgcaacgttgaaggagccactcagccgcgggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggtatccaatggcgttgcaacttcctcggtgagtcggcgcccaaagacctcaaattactcgattcgtgtatgcagtctttggtaataacgcgcaagttttcagcggattccagtgatagtcgatcgtttataaagaacagtttttacgaggtgactgcaaggtatttaaggggagccataggttaBlpIBmtINheISacIIBsu36INOS promoter2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tagagtctcatattcactctcatactgtatttttacaacaattaccaacaacaacaaacaacaaacaacattacaattactatttacaattacaatggaacgagctatacaaggaaacgatgctagggaacaagcttatggtgaacgttggaatggaggatcaggaagttccactatctcagagtataagtgagagtatgacataaaaatgttgttaatggttgttgttgtttgttgtttgttgtaatgttaatgataaatgttaatgttaccttgctcgatatgttcctttgctacgatcccttgttcgaataccacttgcaaccttacctcctagtccttcaaggtgaPsrIHindIIIP19TMV Omega2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tctcccttcaaacttcctgacgaaagtccgagttggactgagtggcggctacataacgatgagactatttcgaatcaagataatccccttggtttcaaggaaagctggggtttcgggaaagttgtatttaagagatatctcagatacgacgggacggaaacttcactgcacagagagagggaagtttgaaggactgctttcaggctcaacctgactcaccgccgatgtattgctactctgataaagcttagttctattaggggaaccaaagttcctttcgaccccaaagccctttcaacataaattctctatagagtctatgctgccctgcctttgaagtgacgtgtctcBstBIEcoRVPshAIP192,6402,6602,6802,7002,7202,7402,7602,7802,800
    tccttggatcttggacgggagattcggttaactatgcagcatctcgatttctcggtttcgaccagatcggatgtacctatagtattcggtttcgaggagttagtgtcaccatttctggagggtcgcgaactcttcagcatctcagtgaaatggcaattcggtctaagcaagaactaggaacctagaacctgccctctaagccaattgatacgtcgtagagctaaagagccaaagctggtctagcctacatggatatcataagccaaagctcctcaatcacagtggtaaagacctcccagcgcttgagaagtcgtagagtcactttaccgttaagccagattcgttcttgaHpaINruIBsaXIBsaXIBseRIBstAPIP192,8202,8402,8602,8802,9002,9202,9402,960
    gctacagcttaccccagtcaaagtggaaagtgatgtatcaagaggatgccctgaaggtgttgaaaccttcgaagaagaaagcgagtaagcttGTCCTGCTTTAATGAGATATGCGAGAAGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTcgatgtcgaatggggtcagtttcacctttcactacatagttctcctacgggacttccacaactttggaagcttcttctttcgctcattcgaaCAGGACGAAATTACTCTATACGCTCTTCGGATACTAGCGTACTATAAACGAAAGTTAAGACAACACGTGCAACATTTTTTGGABstBIBpu10IHindIIIP19OCS terminator2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GAGCATGTGTAGCTCAGATCCTTACCGCCGGTTTCGGTTCATTCTAATGAATATATCACCCGTTACTATCGTATTTTTATGAATAATATTCTCCGTTCAATTTACTGATTGTACCCTACTACTTATATGTACAATATTAAAATGAAAACAATATATTGTGCTGAATAGGTTTATACTCGTACACATCGAGTCTAGGAATGGCGGCCAAAGCCAAGTAAGATTACTTATATAGTGGGCAATGATAGCATAAAAATACTTATTATAAGAGGCAAGTTAAATGACTAACATGGGATGATGAATATACATGTTATAATTTTACTTTTGTTATATAACACGACTTATCCAAATATBsrGIOCS terminator3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GCGACATCTATGATAGAGCGCCACAATAACAAACAATTGCGTTTTATTATTACAAATCCAATTTTAAAAAAAGCGGCAGAACCGGTCAAACCTAAAAGACTGATTACATAAATCTTATTCAAATTTCAAAAGTGCCCCAGGGGCTAGTATCTACGACACACCGAGCGGCGAACTACGCTGTAGATACTATCTCGCGGTGTTATTGTTTGTTAACGCAAAATAATAATGTTTAGGTTAAAATTTTTTTCGCCGTCTTGGCCAGTTTGGATTTTCTGACTAATGTATTTAGAATAAGTTTAAAGTTTTCACGGGGTCCCCGATCATAGATGCTGTGTGGCTCGCCGCTTGATAgeIMfeIPasIOCS terminator3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ATAACGCTCACTGAAGGGAACTCCGGTTCCCCGCCGGCGCGCATGGGTGAGATTCCTTGAAGTTGAGTATTGGCCGTCCGCTCTACCGAAAGTTACGGGCACCATTCAACCCGGTCCAGCACGGCGGCCGGGTAACCGACTTGCTGCCCCGAGAATTATGCAGCATTTTTTTGGTTATTGCGAGTGACTTCCCTTGAGGCCAAGGGGCGGCCGCGCGTACCCACTCTAAGGAACTTCAACTCATAACCGGCAGGCGAGATGGCTTTCAATGCCCGTGGTAAGTTGGGCCAGGTCGTGCCGCCGGCCCATTGGCTGAACGACGGGGCTCTTAATACGTCGTAAAAAAACCAAloIAloIAvaIBsoBIBssHIIBstEIIOCS terminator3,5203,5403,5603,5803,6003,6203,6403,660
    GTATGTGGGCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAGGACCGCTgcaattgtcttctgcacgaagtggtttaaactatcagtgtttgacaggatatattggcgggtaaaCATACACCCGGGGTTTACTTCACGTCCAGTTTGGAACTGTCACTGCTGTTTAGCAACCCGCCCAGGTCCCGCTTAAAACGCTGTTGTACAGCTCCGAGTCGTCCTGGCGAcgttaacagaagacgtgcttcaccaaatttgatagtcacaaactgtcctatataaccgcccatttAhdIApaIBbsIBlpIMfeIPciIPmeIBanIIAflIIIDraIIIPspOMIOCS terminatorRB\short3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cctaagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttccccagatcaggcgctggctgctgaacccccagccggaactgaccccacaaggccctagcgtttgcaatgcaccggattctcttttctcgcaaataatcttattagcctataaattttcccgcacttttccaaataggcaagcaggtaaacatacacgtacggttggtgtcccaaggggtctagtccgcgaccgacgacttgggggtcggccttgactggggtgttccgggatcgcaaacgttacgtggSphISexAIRB\shortRK2\trfa\(no\Esp3I)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aggtcatcattgacccaggcgtgttccaccaggccgctgcctcgcaactcttcgcaggcttcgccgacctgctcgcgccacttcttcacgcgggtggaatccgatccgcacatgaggcggaaggtttccagcttgagcgggtacggctcccggtgcgagctgaaatagtcgaacatccagtagtaactgggtccgcacaaggtggtccggcgacggagcgttgagaagcgtccgaagcggctggacgagcgcggtgaagaagtgcgcccaccttaggctaggcgtgtactccgccttccaaaggtcgaactcgcccatgccgagggccacgctcgactttatcagcttgtAleIRK2\trfa\(no\Esp3I)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tccgtcgggccgtcggcgacagcttgcggtacttctcccatatgaatttcgtgtagtggtcgccagcaaacagcacgacgatttcctcgtcgatcaggacctggcaacgggacgttttcttgccacggtccaggacgcggaagcggtgcagcagcgacaccgattccaggtgcccaggcagcccggcagccgctgtcgaacgccatgaagagggtatacttaaagcacatcaccagcggtcgtttgtcgtgctgctaaaggagcagctagtcctggaccgttgccctgcaaaagaacggtgccaggtcctgcgccttcgccacgtcgtcgctgtggctaaggtccacgggNdeIPfoIRK2\trfa\(no\Esp3I)4,2204,2404,2604,2804,3004,3204,3404,360
    aacgcggtcggacgtgaagcccatcgccgtcgcctgtaggcgcgacaggcattcctcggccttcgtgtaataccggccattgatcgaccagcccaggtcctggcaaagctcgtagaacgtgaaggtgatcggctcgccgataggggtgcgcttcgcgtactccaacacctgctgcttgcgccagcctgcacttcgggtagcggcagcggacatccgcgctgtccgtaaggagccggaagcacattatggccggtaactagctggtcgggtccaggaccgtttcgagcatcttgcacttccactagccgagcggctatccccacgcgaagcgcatgaggttgtggacgacgBsmIPflMIRK2\trfa\(no\Esp3I)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cacaccagttcgtcatcgtcggcccgcagctcgacgccggtgtaggtgatcttcacgtccttgttgacgtggaaaatgaccttgttttgcagcgcctcgcgcgggattttcttgttgcgcgtggtgaacagggcagagcgggccgtgtcgtttggcatcgctcgcatcgtgtccggtgtggtcaagcagtagcagccgggcgtcgagctgcggccacatccactagaagtgcaggaacaactgcaccttttactggaacaaaacgtcgcggagcgcgccctaaaagaacaacgcgcaccacttgtcccgtctcgcccggcacagcaaaccgtagcgagcgtagcacaggcBarIBsaHIPaqCIRK2\trfa\(no\Esp3I)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccacggcgcaatatcgaacaaggaaagctgcatttccttgatctgctgcttcgtgtgtttcagcaacgcggcctgcttggcctcgctgacctgttttgccaggtcctcgccggcggtttttcgcttcttggtcgtcatagttcctcgcgtgtcgatggtcatcgacttcgccaacggtgccgcgttatagcttgttcctttcgacgtaaaggaactagacgacgaagcacacaaagtcgttgcgccggacgaaccggagcgactggacaaaacggtccaggagcggccgccaaaaagcgaagaaccagcagtatcaaggagcgcacagctaccagtagctgaagcggttSfiIBsaBIRK2\trfa\(no\Esp3I)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    acctgccgcctcctgttcaagacgacgcgaacgctccacggcggccgatggcgcgggcagggcagggggagccagttgcacgctgtcgcgctcgatcttggccgtagcttgctggaccatcgagccgacggactggaaggtttcgcggggcgcacgcatgacggtgcggcttgcgtggacggcggaggacaagttctgctgcgcttgcgaggtgccgccggctaccgcgcccgtcccgtccccctcggtcaacgtgcgacagcgcgagctagaaccggcatcgaacgacctggtagctcggctgcctgaccttccaaagcgccccgcgtgcgtactgccacgccgaacgcRK2\trfa\(no\Esp3I)4,9204,9404,9604,9805,0005,0205,0405,060
    atggtttcggcatcctcggcggaaaaccccgcgtcgatcagttcttgcctgtatgccttccggtcaaacgtccgattcattcaccctccttgcgggattgccccgactcacgccggggcaatgtgcccttattcctgatttgacccgcctggtgccttggtgtccagataatccataccaaagccgtaggagccgccttttggggcgcagctagtcaagaacggacatacggaaggccagtttgcaggctaagtaagtgggaggaacgccctaacggggctgagtgcggccccgttacacgggaataaggactaaactgggcggaccacggaaccacaggtctattaggtRK2\trfa\(no\Esp3I)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccttatcggcaatgaagtcggtcccgtagaccgtctggccgtccttctcgtacttggtattccgaatcttgccctgcacgaataccagcgaccccttgcccaaatacttgccgtgggcctcggcctgagagccaaaacacttgatgcggaagaagtcggtgcgctcctgcttgtcggaatagccgttacttcagccagggcatctggcagaccggcaggaagagcatgaaccataaggcttagaacgggacgtgcttatggtcgctggggaacgggtttatgaacggcacccggagccggactctcggttttgtgaactacgccttcttcagccacgcgaggacgaacagAccIRK2\trfa\(no\Esp3I)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gccggcatcgttgcgccacatctaggatctgccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctcggccgtagcaacgcggtgtagatcctagacggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgaDrdIRK2\trf...Esp3I)pUC\ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctggggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacBssSIBssSαIpUC\ori5,6205,6405,6605,6805,7005,7205,7405,760
    cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgpUC\ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gctctgctgaacgagacgacttpU...i5,960
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    5961 bp425045004750500052505500575025050075010001250oriVORI

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    5961