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pGL4.10[luc2]
  • Sequence Map
    Editing disabled on read-only sequence.
    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
    TGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAAACGTGTTTCGGTACTTCGCGATGCGGGACCACGGGCCGTGGTAGCGGAAATGGCTGCGTGTATAGCTCCACCTGTAATGGATGCGGCTCATGAAGCTCTACTCGCAAGCCGACCGTCTTCGATACTTCGCGATACCCGACTTATGTTTGGTAGCCTAGCACCACACGTCGCTCTTluciferase180200220240260280300320340
    TAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGATCGAACGTCAAGAAGTACGGGCACAACCCACGGGACAAGTAGCCACACCGACACCGGGGTCGATTGCTGTAGATGTTGCTCGCGCTCGACGACTTGTCGTACCCGTAGTCGGTCGGGTGGCAGCATAAGCACTCGTTCTTTCCCGACGTTTTCTAGGAGTTGCACGTTTTCTTCluciferase360380400420440460480500520
    CTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGATGGCTAGTATGTTTTCTAGTAGTAGTACCTATCGTTCTGGCTGATGGTCCCGAAGGTTTCGTACATGTGGAAGCACTGAAGGGTAAACGGTGGGCCGAAGTTGCTCATGCTGAAGCACGGGCTCTCGAAGCTGGCCCTGTTTTGGTAGCGGGACTAGTACTTGTCATCACCGTAleIBsaBIBsrGIluciferase540560580600620640660680700
    GTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCATGGCCTAACGGGTTCCCGCATCGGGATGGCGTGGCGTGGCGAACACAGGCTAAGTCAGTACGGGCGCTGGGGTAGAAGCCGTTGGTCTAGTAGGGGCTGTGGCGATAGGAGTCGCACCACGGTAAAGTGGTGCCGAAGCCGTACAAGTGGTGCGACCCGATGAACTAGACGCCStyIBbvCIluciferase720740760780800820840860
    CTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGGAAAGCCCAGCACGAGTACATGGCGAAGCTCCTCCTCGATAAGAACGCGTCGAACGTTCTGATATTCTAAGTTAGACGGGACGACCACGGGTGTGATAAATCGAAGAAGCGATTCTCGTGAGAGTAGCTGTTCATGCTGGATTCGTTGAACGTGCTCTAGCGGTCGCCGCCCCGCFspIKasINarISfoIBseRIBseRIBssSIBssSαIluciferase8809009209409609801,0001,0201,040
    CCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTGGCGAGTCGTTCCTCCATCCACTCCGGCACCGGTTTGCGAAGGTGGATGGTCCGTAGGCGGTCCCGATGCCGGACTGTCTTTGTTGGTCGCGGTAAGACTAGTGGGGGCTTCCCCTGCTGTTCGGACCGCGTCATCCGTTCCACCACGGGAAGAAGCTCCGATTCCACCACCTGABlpIPluTIluciferase1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATACCTGTGGCCATTCTGTGACCCACACTTGGTCGCGCCGCTCGACACGCAGGCACCGGGGTACTAGTACTCGCCGATGCAATTGTTGGGGCTCCGATGTTTGCGAGAGTAGCTGTTCCTGCCGACCGACGTGTCGCCGCTGTAGCGGATGACCCTGCTCCTGCTCGTGAAGAAGTAAgeIHpaIDraIIIluciferase1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCGCACCTGGCCGACTTCTCGGACTAGTTTATGTTCCCGATGGTCCATCGGGGTCGGCTTGACCTCTCGTAGGACGACGTTGTGGGGTTGTAGAAGCTGCGGCCCCAGCGGCCGGACGGGCTGCTGCTACGGCCGCTCGACGGGCGGCGTCAGCAGCACGACCTTGTGCCATTTTGGBpmIDrdISapIBspQISexAIluciferase1,4201,4401,4601,4801,5001,5201,5401,560
    ATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGTTACTGGCTCTTCCTCTAGCACCTGATACACCGGTCGGTCCAATGTTGGCGGTTCTTCGACGCGCCACCACAACACAAGCACCTGCTCCACGGATTTCCTGACTGGCCGTTCAACCTGCGGGCGTTCTAGGCGCTCTAAGAGTAATTCCGGTTCTTCCCGCCGTTCTAGCGGCACAluciferase1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AATAATTCTAGAGTCGGGGCGGCCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAATTATTAAGATCTCAGCCCCGCCGGCCGGCGAAGCTCGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTAATATTCGACGTTATTTGTTCAATTGTTGTTApoIBsmIFseIHpaIPsiIXbaISV40 poly(A) signal1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCGTTAACGTAAGTAAAATACAAAGTCCAAGTCCCCCTCCACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATTTTAGCTATTCCTAGGCAGCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCCGCGCCCCGTACTGATAGCAGCGGCGAccIBsmIClaIMfeISalIBamHIBsaBIBspDIPshAI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAATGAATACTGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTBbsISapIBspQI2,1202,1402,1602,1802,2002,2202,2402,260
    CATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGDrdIPciIBssSIAflIIIBssSαIori2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAApaLIBciVIori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGAAlwNIori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACCGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGori2,8202,8402,8602,8802,9002,9202,9402,960
    TCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCGGCCGCAAATGCTAAACCACTGCAGTGGTTACCAGTGCTTGATCAGTGAGGCACCAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCGCCGGCGTTTACGATTTGGTGACGTCACCAATGGTCACGAACTAGTCACTCCGTGGAleINotIPstIBstXIBstEIIAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GATCTCAGCGATCTGCCTATTTCGTTCGTCCATAGTGGCCTGACTCCCCGTCGTGTAGATCACTACGATTCGTGAGGGCTTACCATCAGGCCCCAGCGCAGCAATGATGCCGCGAGAGCCGCGTTCACCGGCCCCCGATTTGTCAGCAATGAACCAGCCAGCAGGGAGGGCCGAGCTAGAGTCGCTAGACGGATAAAGCAAGCAGGTATCACCGGACTGAGGGGCAGCACATCTAGTGATGCTAAGCACTCCCGAATGGTAGTCCGGGGTCGCGTCGTTACTACGGCGCTCTCGGCGCAAGTGGCCGGGGGCTAAACAGTCGTTACTTGGTCGGTCGTCCCTCCCGGCTCAhdIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CGAAGAAGTGGTCCTGCTACTTTGTCCGCCTCCATCCAGTCTATGAGCTGCTGTCGTGATGCTAGAGTAAGAAGTTCGCCAGTGAGTAGTTTCCGAAGAGTTGTGGCCATTGCTACTGGCATCGTGGTATCACGCTCGTCGTTCGGTATGGCTTCGTTCAACTCTGGTTCCCAGCGCTTCTTCACCAGGACGATGAAACAGGCGGAGGTAGGTCAGATACTCGACGACAGCACTACGATCTCATTCTTCAAGCGGTCACTCATCAAAGGCTTCTCAACACCGGTAACGATGACCGTAGCACCATAGTGCGAGCAGCAAGCCATACCGAAGCAAGTTGAGACCAAGGGTCGAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GGTCAAGCCGGGTCACATGATCACCCATATTATGAAGAAATGCAGTCAGCTCCTTAGGGCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCGGTGTTGTCGCTCATGGTAATGGCAGCACTACACAATTCTCTTACCGTCATGCCATCCGTAAGATGCTTTTCCGTGACCGGCGACCAGTTCGGCCCAGTGTACTAGTGGGTATAATACTTCTTTACGTCAGTCGAGGAATCCCGGAGGCTAGCAACAGTCTTCATTCAACCGGCGCCACAACAGCGAGTACCATTACCGTCGTGATGTGTTAAGAGAATGGCAGTACGGTAGGCATTCTACGAAAAGGCACTGGCCGCTPvuISacIIBsu36IAmpR3,5203,5403,5603,5803,6003,6203,6403,660
    GTACTCAACCAAGTCGTTTTGTGAGTAGTGTATACGGCGACCAAGCTGCTCTTGCCCGGCGTCTATACGGGACAACACCGCGCCACATAGCAGTACTTTGAAAGTGCTCATCATCGGGAATCGTTCTTCGGGGCGGAAAGACTCAAGGATCTTGCCGCTATTGAGATCCAGTTCGCATGAGTTGGTTCAGCAAAACACTCATCACATATGCCGCTGGTTCGACGAGAACGGGCCGCAGATATGCCCTGTTGTGGCGCGGTGTATCGTCATGAAACTTTCACGAGTAGTAGCCCTTAGCAAGAAGCCCCGCCTTTCTGAGTTCCTAGAACGGCGATAACTCTAGGTCAAGCAccIBstZ17IAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATATAGCCCACTCTTGCACCCAGTTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCGGGGTGTGCAAAAACAGGCAAGCAAAATGCCGCAAAGAAGGGAATGAGTGCGACACGAAAATGTTGGATGCTCATACTCGTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTTATATCGGGTGAGAACGTGGGTCAACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGCCCCACACGTTTTTGTCCGTTCGTTTTACGGCGTTTCTTCCCTTACTCACGCTGTGCTTTTACAACCTACGAGTATGAGCAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAASspIAmpRAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ACTAGTACGTCTCTCAAGGATAAGTAAGTAATATTAAGGTACGGGAGGTATTGGACAGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAACGAAACAAAACAAACTAGCAAAATTGATCATGCAGAGAGTTCCTATTCATTCATTATAATTCCATGCCCTCCATAACCTGTCCGGCGTTATTTTATAGAAATAAAAGTAATGTAGACACACAACCAAAAAACACACTTAGCTATCATGATTGTATGCGAGAGGTAGTTTTGTTTTGCTTTGTTTTGTTTGATCGTTTTAClaISpeISspIBcoDIBsmAIBsmBIBspDIEsp3Ipoly(A) signalpause site4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTTCCGACAGGGGTCACGTTCACGTCCACGGTCTTGTAAAGAGABfuAIBspMIPaqCIpause site4,2204,240
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4242 bp300032503500375040002505007501000

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    4242