Welcome to Benchling

Benchling is an intelligent research platform with tools for note-taking, molecular biology, and sample tracking.

Create an account

Email
Already have an account?
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pGL3-Control
  • Sequence Map
    Editing disabled on read-only sequence.
    GGTACCGAGCTCTTACGCGTGCTAGCCCGGGCTCGAGATCTGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGCCATGGCTCGAGAATGCGCACGATCGGGCCCGAGCTCTAGACGCTAGACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTAGCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCBmtIKpnIMluINheISacISmaISrfITliIXhoIXmaIBglIIBsaBIBtgZITspMIAcc65IPaeR7IEco53kISV40 promoterMCS20406080100120140160
    GCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTGGAAGATGGAACCGCTGGAGACGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTTCGAACCGTAAGGCCATGACAACCATTTCGGTGGTACCTTCTGCGGTTTTTGTATTTCTTTCCGGGCCGCGGTAAGATAGGCGACCTTCTACCTTGGCGACCTCTBtgIKasINarINcoISfiISfoIStuIStyIStyIAvrIIPluTIHindIIIluciferaseSV40 promoter180200220240260280300320340
    GCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGGACATCACTTACGCTGAGTACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTCGTTGACGTATTCCGATACTTCTCTATGCGGGACCAAGGACCTTGTTAACGAAAATGTCTACGTGTATAGCTCCACCTGTAGTGAATGCGACTCATGAAGCTTTACAGGCAAGCCAACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTTAGCAGCATACGTCAPfoIBstBIluciferase360380400420440460480500520
    GAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAACTTTTGAGAGAAGTTAAGAAATACGGCCACAACCCGCGCAATAAATAGCCTCAACGTCAACGCGGGCGCTTGCTGTAAATATTACTTGCACTTAACGAGTTGTCATACCCGTAAAGCGTCGGATGGCACCACAAGCAAAGGTTTTTCCCCAACGTTTTTTAAAACTTGCACGTTTBtgIluciferase540560580600620640660680700
    AAAAGCTCCCAATCATCCAAAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTGCCAGAGTCCTTCGATAGGGACAAGACAATTGCACTGATCATGAACTCCTCTTTTCGAGGGTTAGTAGGTTTTTTAATAATAGTACCTAAGATTTTGCCTAATGGTCCCTAAAGTCAGCTACATGTGCAAGCAGTGTAGAGTAGATGGAGGGCCAAAATTACTTATGCTAAAACACGGTCTCAGGAAGCTATCCCTGTTCTGTTAACGTGACTAGTACTTGAGGAGBclIBsrGIluciferase720740760780800820840860
    TGGATCTACTGGTCTGCCTAAAGGTGTCGCTCTGCCTCATAGAACTGCCTGCGTGAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATAACCTAGATGACCAGACGGATTTCCACAGCGAGACGGAGTATCTTGACGGACGCACTCTAAGAGCGTACGGTCTCTAGGATAAAAACCGTTAGTTTAGTAAGGCCTATGACGCTAAAATTCACAACAAGGTAAGGTAGTGCCAAAACCTTACAAATGATGTGAGCCTATAAACTATXcmIBspEIluciferase8809009209409609801,0001,0201,040
    TGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTCTGAGGAGCCTTCAGGATTACAAGATTCAAAGTGCGCTGCTGGTGCCAACCCTATTCTCCTTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGTGACACCTAAAGCTCAGCAGAATTACATATCTAAACTTCTTCTCGACAAAGACTCCTCGGAAGTCCTAATGTTCTAAGTTTCACGCGACGACCACGGTTGGGATAAGAGGAAGAAGCGGTTTTCGTGAGACTAACTGTTTATGCTAAATAGATTAAATGTGCTTTAACGAAGACCACSapIBspQIluciferase1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GCGCTCCCCTCTCTAAGGAAGTCGGGGAAGCGGTTGCCAAGAGGTTCCATCTGCCAGGTATCAGGCAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTCGCGAGGGGAGAGATTCCTTCAGCCCCTTCGCCAACGGTTCTCCAAGGTAGACGGTCCATAGTCCGTTCCTATACCCGAGTGACTCTGATGTAGTCGATAAGACTAATGTGGGCTCCCCCTACTATTTGGCCCGCGCCAGCCATTTCAACAAGGTAAAAAACTTCGCTTCCAACAluciferase1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAAAGAGGCGAACTGTGTGTGAGAGGTCCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCCCTAGACCTATGGCCCTTTTGCGACCCGCAATTAGTTTCTCCGCTTGACACACACTCTCCAGGATACTAATACAGGCCAATACATTTGTTAGGCCTTCGCTGGTTGCGGAACTAACTGTTCCTACCTACCGATGTAAGACCTCTGTATCGAATGACCCTGCTTCTGCTTGTGAAGBspEIPpuMIEcoO109Iluciferase1,4201,4401,4601,4801,5001,5201,5401,560
    TTCATCGTTGACCGCCTGAAGTCTCTGATTAAGTACAAAGGCTATCAGGTGGCTCCCGCTGAATTGGAATCCATCTTGCTCCAACACCCCAACATCTTCGACGCAGGTGTCGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAAGTAGCAACTGGCGGACTTCAGAGACTAATTCATGTTTCCGATAGTCCACCGAGGGCGACTTAACCTTAGGTAGAACGAGGTTGTGGGGTTGTAGAAGCTGCGTCCACAGCGTCCAGAAGGGCTGCTACTGCGGCCACTTGAAGGGCGGCGGCAACAACAAAACCTCGTGCCTTPaqCISgrAIluciferase1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCTCTGCTACTGCCTTTTTCTCTAGCACCTAATGCAGCGGTCAGTTCATTGTTGGCGCTTTTTCAACGCGCCTCCTCAACACAAACACCTGCTTCATGGCTTTCCAGAATGGCCTTTTGAGCTGCGTTCTTTTTAGTCTCTCTAGGAGTATTTCCGGTTCTTCCCGCCTTTCTAGCGluciferase1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CGTGTAATTCTAGAGTCGGGGCGGCCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACGCACATTAAGATCTCAGCCCCGCCGGCCGGCGAAGCTCGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTAATATTCGACGTTATTTGTTCAATTGTTGFseIHpaIXbaISV40 poly(A) signal1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCTGAACGATGGAGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGGCGGGATGGGCGGAGTTAGGGGCGGGACTATTTGTTAACGTAAGTAAAATACAAAGTCCAAGTCCCCCTCCACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATTTTAGCTATTCCTAGACTTGCTACCTCGCCTCTTACCCGCCTTGACCCGCCTCAATCCCCGCCCTACCCGCCTCAATCCCCGCCCTGATABsaBISV40 enhancer2,1202,1402,1602,1802,2002,2202,2402,260
    GGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCTGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGCGGATCCGTCCCAACGACTGATTAACTCTACGTACGAAACGTATGAAGACGGACGACCCCTCGGACCCCTGAAAGGTGTGGACCAACGACTGATTAACTCTACGTACGAAACGTATGAAGACGGACGACCCCTCGGACCCCTGAAAGGTGTGGGATTGACTGTGTGTAAGGTGTCGCCTAGGCAGAccINsiINsiISalIBamHISexAIBstAPIBstAPISV40 enhancer2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCCGCGCCCCGTACTGATAGCAGCGGCGTGAATACTGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGAfeISapIBspQIPshAI2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCPciIori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGBssSIBssSαIori2,8202,8402,8602,8802,9002,9202,9402,960
    AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCAlwNIApaLIBaeGIori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCori3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCAhdIBsaIBsrDIAmpR3,5203,5403,5603,5803,6003,6203,6403,660
    CTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGAclIAseIFspIBsrDIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAPvuIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCAclIXmnIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAApaLIBaeGIBssSIBssSαIAmpRAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    CATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACAmpR promoterf1 ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAAf1 ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTTCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAAAloIBsaAIBtgZIDraIIIf1 ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    AAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCCCAAGCTACCATGATAAGTAAGTAATATTAAGGTACGGTTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAACGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGGGTTCGATGGTACTATTCATTCATTATAATTCCATGCCFspIPvuIf1 ori4,9204,9404,9604,9805,0005,0205,0405,060
    GAGGTACTTGGAGCGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAACGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTACTCCATGAACCTCGCCGGCGTTATTTTATAGAAATAAAAGTAATGTAGACACACAACCAAAAAACACACTTAGCTATCATGATTGTATGCGAGAGGTAGTTTTGTTTTGCTTTGTTTTGTTTGATCGTTTTATCCGACAGGGGTCACGTTCACGTCCACGGTCTTGTAAAGAGATBsgINotIPaqCIpause sitepoly(A) signal5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TCGATAAGCTAT5,255
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5256 bp37504000425045004750500025050075010001250MCS,SV40 promoter,Acc65I,KpnI,Eco53kI,SacI,MluI,NheI,BmtI,TspMI,XmaI,SmaI,SrfI,+18PfoI,BstBIBtgIBsrGIBclIBspEI,XcmI,BspQI,SapIluciferaseAmpRPvuIAclI,XmnI,BssSI,BssSαI,ApaLI,BaeGIAmpRAmpR promoterf1 ori,BtgZI,BsaAI,DraIII,AloIpoly(A) signal,pause site,FspI,PvuI,NotI,PaqCI,BsgI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    5256