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pGL2-Basic
  • Sequence Map
    Editing disabled on read-only sequence.
    CCCGGGAGGTACCGAGCTCTTACGCGTGCTAGCTCGAGATCTAAGTAAGCTTGGCATTCCGGTACTGTTGGTAAAATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGGGGCCCTCCATGGCTCGAGAATGCGCACGATCGAGCTCTAGATTCATTCGAACCGTAAGGCCATGACAACCATTTTACCTTCTGCGGTTTTTGTATTTCTTTCCGGGCCGCGGTAAGATAGGAGATCTCCTACCTTGGCGACCTCTCGTTGACGTATTCCGATACTTCTCTATGCBmtIKasIKpnIMluINarINheISacISfoISmaITliIXbaIXhoIXmaIBglIIPluTITspMIAcc65IPaeR7IEco53kIHindIIIluciferaseMCS20406080100120140160
    CCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGGGACCAAGGACCTTGTTAACGAAAATGTCTACGTGTATAGCTCCACTTGTAGTGCATGCGCCTTATGAAGCTTTACAGGCAAGCCAACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTTAGCAGCATACGTCACTTTTGAGAGAAGTTAAGAAATACGGCCAPfoIPsrIBsiWIBstBIluciferase180200220240260280300320340
    GTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTCAACCCGCGCAATAAATAGCCTCAACGTCAACGCGGGCGCTTGCTGTAAATATTACTTGCACTTAACGAGTTGTCATACTTGTAAAGCGTCGGATGGCATCACAAACAAAGGTTTTTCCCCAACGTTTTTTAAAACTTGCACGTTTTTTTTAATGGTTATTAGGTCTTTTAATAAluciferase360380400420440460480500520
    ATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGTAGTACCTAAGATTTTGCCTAATGGTCCCTAAAGTCAGCTACATGTGCAAGCAGTGTAGAGTAGATGGAGGGCCAAAATTACTTATGCTAAAACATGGTCTCAGGAAACTAGCACTGTTTTGTTAACGTGACTATTACTTAAGGAGACCTAGATGACCCAATGGATTCCCACACCTatIBsrGIEcoRIBstEIIBsu36Iluciferase540560580600620640660680700
    CCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGGGGAAGGCGTATCTTGACGGACGCAGTCTAAGAGCGTACGGTCTCTAGGATAAAAACCGTTAGTTTAGTAAGGCCTATGACGCTAAAATTCACAACAAGGTAAGGTAGTGCCAAAACCTTACAAATGATGTGAGCCTATAAACTATACACCTAAAGCTCAGCAGAATTACATATCNspISphIXcmIBspEIluciferase720740760780800820840860
    ATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAATAAACTTCTTCTCGACAAAAATGCTAGGGAAGTCCTAATGTTTTAAGTTTCACGCAACGATCATGGTTGGGATAAAAGTAAGAAGCGGTTTTCGTGAGACTAACTGTTTATGCTAAATAGATTAAATGTGCTTTAACGAAGACCCCCGCGTGGAGAAAGCTTTCTTCAGCCCCTTAjuISapIBspQIBstBIluciferase8809009209409609801,0001,0201,040
    GCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGCGCCAACGTTTTGCGAAGGTAGAAGGTCCCTATGCTGTTCCTATACCCGAGTGACTCTGATGTAGTCGATAAGACTAATGTGGGCTCCCCCTACTATTTGGCCCGCGCCAGCCATTTCAACAAGGTAAAAAACTTCGCTTCCAACACCTAGACCTATGGCCCTTTTGCGACCCGCBseYIluciferase1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATAATTAGTCTCTCCGCTTAATACACAGTCTCCTGGATACTAATACAGGCCAATACATTTGTTAGGCCTTCGCTGGTTGCGGAACTAACTGTTCCTACCTACCGATGTAAGACCTCTGTATCGAATGACCCTGCTTCTGCTTGTGAAGAAGTATCAACTGGCGAACTTCAGAAATTABsmFIBspEIPpuMIEcoO109Iluciferase1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATATTTATGTTTCCTATAGTCCACCGGGGGCGACTTAACCTTAGCTATAACAATGTTGTGGGGTTGTAGAAGCTGCGCCCGCACCGTCCAGAAGGGCTGCTACTGCGGCCACTTGAAGGGCGGCGGCAACAACAAAACCTCGTGCCTTTCTGCTACTGCCTTTTTCTCTAGCACCTAClaIPacIBfuAIBspDIBspMIEcoRVSgrAIluciferase1,4201,4401,4601,4801,5001,5201,5401,560
    TACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAAAATGTAACTGTATTCAGCGATGATGCAGCGGTCAGTTCATTGTTGGCGCTTTTTCAACGCGCCTCCTCAACACAAACACCTGCTTCATGGCTTTCCAGAATGGCCTTTTGAGCTGCGTTCTTTTTAGTCTCTCTAGGAGTATTTCCGGTTCTTCCCGCCTTTCAGGTTTAACATTTTACATTGACATAAGTCGCTACBplIBplIBseRIEcoNIluciferase1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ACGAAATTCTTAGCTATTGTAATACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGTTGCTACGCCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGCCGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTTGCTTTAAGAATCGATAACATTATGACGCTACTCACCGTCCCGCCCCGCATTAAAAAAATTCCGTCAATAACCACGGGAATTTGCGGACCAACGATGCGGACTTATTCACTATTATTCGCCTACTTACCGTCTTTAAGCGGCCTAGAAACACTTCCTTGGAATGAAGACACCACABarI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GACATAATTGGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTATAATGTGTTAAACTACTGATTCTAATTGTTTGTGTATTTTAGATTCCAACCTATGGAACTGATGAATGGGAGCAGTGGTGGAATGCCTTTAATGAGGAAAACCTGTTTCTGTATTAACCTGTTTGATGGATGTCTCTAAATTTCGAGATTCCATTTATATTTTAAAAATTCACATATTACACAATTTGATGACTAAGATTAACAAACACATAAAATCTAAGGTTGGATACCTTGACTACTTACCCTCGTCACCACCTTACGGAAATTACTCCTTTTGGACAAAPflMIsmall t intron1,9401,9601,9802,0002,0202,0402,0602,0802,100
    TGCTCAGAAGAAATGCCATCTAGTGATGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAGAAGAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGCTAAGTTTTTTGAGTCATGCTGTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACAACGAGTCTTCTTTACGGTAGATCACTACTACTCCGATGACGACTGAGAGTTGTAAGATGAGGAGGTTTTTTCTTCTCTTTCCATCTTCTGGGGTTCCTGAAAGGAAGTCTTAACGATTCAAAAAACTCAGTACGACACAAATCATTATCTTGAGAACGAACGAAACGATAAATGTStyIBseRI2,1202,1402,1602,1802,2002,2202,2402,260
    CCACAAAGGAAAAAGCTGCACTGCTATACAAGAAAATTATGGAAAAATATTCTGTAACCTTTATAAGTAGGCATAACAGTTATAATCATAACATACTGTTTTTTCTTACTCCACACAGGCATAGAGTGTCTGCTATTAATAACTATGCTCAAAAATTGTGTACCTTTAGCTTTTTGGTGTTTCCTTTTTCGACGTGACGATATGTTCTTTTAATACCTTTTTATAAGACATTGGAAATATTCATCCGTATTGTCAATATTAGTATTGTATGACAAAAAAGAATGAGGTGTGTCCGTATCTCACAGACGATAATTATTGATACGAGTTTTTAACACATGGAAATCGAAAAAAseIBsgI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AATTTGTAAAGGGGTTAATAAGGAATATTTGATGTATAGTGCCTTGACTAGAGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTAAACATTTCCCCAATTATTCCTTATAAACTACATATCACGGAACTGATCTCTAGTATTAGTCGGTATGGTGTAAACATCTCCAAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGTATTTTACTTACGTTAACAACAACAATTGAACAAATAACGTCGHpaIBsaBISV40 ...gnal2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCTAGGCAGCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCCGCGCCAccISalIBamHISV40 poly(A) signal2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCACCGTACTGATAGCAGCGGCGTGAATACTGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTAfeINaeISapIBspQIPshAINgoMIV2,8202,8402,8602,8802,9002,9202,9402,960
    GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCADrdINspIPciIori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAApaLIBseYIBssSIBssSαIori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTAlwNIori3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACori3,5203,5403,5603,5803,6003,6203,6403,660
    ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGBspHIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCAAhdIBglIBsaIBsrDIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCCCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGAclIAseIFspIBsrDINmeAIIIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGEaeIPvuIScaITatIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGAclIXmnIAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTApaLIBspHIBssSIBssSαIAmpRAmpRAmpR promoter4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAf1 oriAmpR promoter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGANaeIDraIIINgoMIVf1 ori4,9204,9404,9604,9805,0005,0205,0405,060
    TTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTAloIDrdIf1 ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CGCTTACAATTTGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCCCAAGCTACCATGATAAGTAAGTAATATTAAGGTACGTGGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACGCGAATGTTAAACGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGGGTTCGATGGTACTATTCATTCATTATAATTCCATGCACCTCCAAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGBglIFspIPvuIf1 ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATGGTACTGTAACTGAGCTAACATAATATTTTACTTACGTTAACAACAACAATTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATACCATGACATTGACTCGATTGTATTHpaISV40 poly(A) signal5,4405,4605,4805,5005,5205,5405,5605,580
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5598 bp40004250450047505000525025050075010001250MCS,TspMI,XmaI,SmaI,Acc65I,KpnI,Eco53kI,SacI,MluI,NheI,BmtI,PaeR7I,TliI,XhoI,+11BsrGI,TatIEcoRI,BstEII,Bsu36I,NspI,SphIluciferase,BspEI,XcmI,BspQI,SapIAjuIBstBIBseYI,EcoO109I,PpuMIBspEIBsmFIAmpR,PvuI,EaeITatI,ScaIAclI,XmnI,BssSI,BssSαI,ApaLIAmpRAmpR promoter,BspHINgoMIV,NaeIf1 ori,DraIII,DrdI,AloISV40 poly(A) signal,BglI,FspI,PvuI,HpaI

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    5598