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pMSx64
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    tcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgcagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgSalIHincIIBstZ17I20406080100120140160
    tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaaagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattBsaXI180200220240260280300320340
    tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtccPciIpUC ori360380400420440460480500520
    actataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgpUC ori540560580600620640660680700
    tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgaAlwNIApaLIpUC ori720740760780800820840860
    acggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgattgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactapUC ori8809009209409609801,0001,0201,040
    cttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcaBspHIAmpRpUC ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttAhdIBsaIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccBglIFspIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    ttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacacPvuIAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    actggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagattgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaBcgIScaITatIXmnIAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatSspIApaLIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctccACGCGTGGTACCCTCGAGgcgatctgcatctcaattagtcagcaaccatagtcagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagaggTGCGCACCATGGGAGCTCcgctagacgtagagttaatcagtcgttggtatcagKpnIMluISalITliIXhoIZraIAatIIBglIIBspHIPspXISgrDIAcc65IHincIIPaeR7IAmpRSV40 early promoter2,1202,1402,1602,1802,2002,2202,2402,260
    ccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatcgctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaagagctcggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtagcgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgttttctcgagBglISacISfiIAvrIIBseRIEco53kISV40 early promoter2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tctggctaactagagaacccactgcttactggcttatcgaaattaatacgactcactatagggagacccaagctgTTCGAAGCGGAATTCACCATGACTAGTggtggttctggtatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggagaccgattgatctcttgggtgacgaatgaccgaatagctttaattatgctgagtgatatccctctgggttcgacAAGCTTCGCCTTAAGTGGTACTGATCAccaccaagaccatacgttctcttgatgttctaagaccaccagctactgctgtacgcggacgcacgcgacgaccBsaISpeIEcoRIMT2SG4GS6GM8QE10NY12KI14LV16VD18DD20MR22LR24AL26L2544-3560OmpR-OGRompR CDST7 prom2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgcctgctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatttgcaatagagtggcttgttccgaaggtccaagcttcgcagcgattacgtcttgtctacctagcggacgactgagcacttagaaaggtagaataccatgacctaaattacaatggaccacttctaccgaacagctaaacggctgcagaagcatcagtctcgttgggctacggctaAloIZraIAatIIPflMISexAIE28RY30LT32EQ34GF36QV38RS40VA42NA44EQ46MD48RL50LT52RE54SF56HL58MV60LD62LM64LP66GE68DG70LS72IC74RR76LR78SQ80SN82PM84PI862544-3560OmpR-OGRompR CDS2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtagtaataccactgccgctttccccttcttcacctggcatagcatccggacctctaaccgcgactgctgatgtaaggttttggcaaattgggcgcacttgacgaccgggcataggcacgccacgacgcagcagtccgcttgcttgacggtccgcgtggcagtgtccttctccgccatBcgIIM88VT90AK92GE94EV96DR98IV100GL102EI104GA106DD108YI110PK112PF114NP116RE118LL120AR122IR124AV126LR128RQ130AN132EL134PG136AP138SQ140EE142AV1442544-3560OmpR-OGRompR CDS2,8202,8402,8602,8802,9002,9202,9402,960
    attgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcTATaagctgatgaaccttgcccgtggtcgtgaatattccgtaacgaaagccattcaagtttgaattggagccatgcgcgctttacaaggcgcttctgctcggctacggcgagtggtcgccactcaaacgccatgacttccgtgaccagtcggtaggcgcactcggcgagagggcgATAttcgactacttggaacgggcaccagcacttataaggcSspIIA146FG148KF150KL152NL154GT156RE158MF160RE162DE164PM166PL168TS170GE172FA174VL176KA178LV180SH182PR184EP186LS188RY190KL192MN194LA196RG198RE200YS2022544-3560OmpR-OGRompR CDS2,9803,0003,0203,0403,0603,0803,1003,1203,140
    caatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagCTAGTggtggttctggtgcccccccgaccgatgtcagcctgggggagttaccttgcgaggtagctgcacgtctaaagcgcagacgcggcgtaccaccttcttctaggtcgcgtaggcgcaatgtaagtctggcagaccccagacccgatgcagaaacatggcctgccgagatttcGATCAccaccaagaccacgggggggctggctacagtcggaccccctBsgIBmgBIPshAIAM204ER206SI208DV210QI212SR214LR216RM218VE220ED222PA224HP226RY228IQ230TV232WG234LG236YV238FV240PD242GS244KA246SG248GS250GA252PP254TD256VS258LG260D2544-3560OmpR-OGRompR CDSVP163,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgctcgaggtgaatctgccgctcctgcaccgctaccgcgtacggctgcgcgatctgctaaagctagacctgtacaaccccctgcccctaaggggcccaggccctaaatggggggtgctgaggcgggggatgccgcgagacctataccggctgaagctcaaactcgtctacaaatggPciIPfoISacISmaIXmaIBmgBITspMIEco53kIE262LH264LD266GE268DV270AM272AH274AD276AL278DD280FD282LD284ML286GD288GD290SP292GP294GF296TP298HD300SA302PY304GA306LD308MA310DF312EF314EQ316MF318T2544-3560OmpR-OGRVP163,3403,3603,3803,4003,4203,4403,4603,4803,500
    gatgcccttggaattgacgagtacggtgggGCTAGTgctagcgGATCCACCGGTgTCTAGAAAGCTTtgaGGCCGGcctgcagggatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccacctacgggaaccttaactgctcatgccacccCGATCAcgatcgcCTAGGTGGCCAcAGATCTTTCGAAactCCGGCCggacgtccctagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgAgeIBmtIFseINheIPstISbfIXbaIBamHISgrAIHindIIID320AL322GI324DE326YG328GA330SA332SG334ST336GV338*2544-3560BGH polyAOmpR-OGRVP163,5203,5403,5603,5803,6003,6203,6403,660
    tgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggcacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgBGH polyA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggcagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgf1 origin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaacccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggAloIBsaAIBsaXIDraIIIf1 origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatcatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagNsiIPsiIXmnIBstAPIf1 originSV404,2204,2404,2604,2804,3004,3204,3404,360
    tcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttttagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaNcoINsiISexAIBstAPISV404,3804,4004,4204,4404,4604,4804,5004,5204,540
    tatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagttctctgtcctactcctagcaaagcgtactaacttgttctacctaacBclISmaIXmaIAvrIIBseRITspMISV40NeoR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcgtgcgtccaagaggccggcgaacccacctctccgataagccgatactgacccgtgttgtctgttagccgacgagactacggcggcacaaggccgacagtcgcgtccccgcgggccaagaaaaacagttctggctggacaggccacgggacttacttgacgtcctgctccgtcgcgEagIKasINarIPstISfoIPluTINeoR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcaccgatagcaccgaccggtgctgcccgcaaggaacgcgtcgacacgagctgcaacagtgacttcgcccttccctgaccgacgataacccgcttcacggccccgtcctagaggacagtagagtggaacgaggacggctctttcataggtagtaccgactacgttacgccgccgacgtFspIMscIPflFITth111INeoR4,9204,9404,9604,9805,0005,0205,0405,060
    tacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgagatgcgaactaggccgatggacgggtaagctggtggttcgctttgtagcgtagctcgctcgtgcatgagcctaccttcggccagaacagctagtcctactagacctgcttctcgtagtccccgagcgcggtcggcttgacaagcggtccgagttccgcgcgtacgggctgccgctcBsaAIBssHIINeoR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttccctagagcagcactgggtaccgctacggacgaacggcttatagtaccaccttttaccggcgaaaagacctaagtagctgacaccggccgacccacaccgcctggcgatagtcctgtatcgcaaccgatgggcactataacgacttctcgaaccgccgcttacccgactggcgaaggNcoIRsrIINeoR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaagcacgaaatgccatagcggcgagggctaagcgtcgcgtagcggaagatagcggaagaactgctcaagaagactcgccctgagaccccaagctttactggctggttcgctgcgggttggacggtagtgctctaaagctaaggtggcggcggaagatactttccaacccgaagcctNeoR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccatagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtBsmIPfoIPsiISV40 polyA5,6205,6405,6605,6805,7005,7205,7405,760
    aactcatcaatgtatcttattgagtagttacatagaatSV40 polyA5,780
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    5794 bp42504500475050005250550025050075010001250AmpRNeoR

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    5794