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pCEV-G2-Km
  • Sequence Map
    Editing disabled on read-only sequence.
    ggagcgacctcatgctatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcttatttagcctcgctggagtacgatatggactctttcgttggactggatgtcctttctcaatgagttcttattcttaaaagcaaaattttggattctcagtgaaattttaaacatatgtgaataaaaaaaatattgaataaattattatttttagtatttagtattctttaagcgaataaatcPsiIBstZ17IADH1 terminatorpHybLex-Rsource20406080100120140160
    aagtgtcaacaacgtatctaccaacgatttgacccttttccatcttttcgtaaatttctggcaaggtagacaagccgacaaccttgattggagacttgaccaaacctctggcgaagaattgttaattaagagctcagatcttatcgtcgtcatccttgtaatccatcgatactagttcacagttgttgcatagatggttgctaaactgggaaaaggtagaaaagcatttaaagaccgttccatctgttcggctgttggaactaacctctgaactggtttggagaccgcttcttaacaattaattctcgagtctagaatagcagcagtaggaacattaggtagctatgatcBcgIPacISacISpeIBglIIHincIIEco53kIFLAGsource180200220240260280300320340
    tgcggccgcttgttttatatttgttgtaaaaagtagataattacttccttgatgatctgtaaaaaagagaaaaagaaagcatctaagaacttgaaaaactacgaattagaaaagaccaaatatgtatttcttgcattgaccaatttatgcaagtttatatatatgtaaatgtaagacgccggcgaacaaaatataaacaacatttttcatctattaatgaaggaactactagacattttttctctttttctttcgtagattcttgaactttttgatgcttaatcttttctggtttatacataaagaacgtaactggttaaatacgttcaaatatatatacatttacattcEagINotIsource360380400420440460480500520
    tttcacgaggttctactaaactaaaccacccccttggttagaagaaaagagtgtgtgagaacaggctgttgttgtcacacgattcggacaattctgtttgaaagagagagagtaacagtacgatcgaacgaactttgctctggagatcacagtgggcatcatagcatgtggtactaaagtgctccaagatgatttgatttggtgggggaaccaatcttcttttctcacacactcttgtccgacaacaacagtgtgctaagcctgttaagacaaactttctctctctcattgtcatgctagcttgcttgaaacgagacctctagtgtcacccgtagtatcgtacaccatgaBpmIStyIsource540560580600620640660680700
    aaaccctttcccgccattccagaaccttcgattgcttgttacaaaacctgtgagccgtcgctaggaccttgttgtgtgacgaaattggaagctgcaatcaataggaagacaggaagtcgagcgtgtctgggttttttcagttttgttctttttgcaaacaaatcacgagcgacggtttgggaaagggcggtaaggtcttggaagctaacgaacaatgttttggacactcggcagcgatcctggaacaacacactgctttaaccttcgacgttagttatccttctgtccttcagctcgcacagacccaaaaaagtcaaaacaagaaaaacgtttgtttagtgctcgctgccAhdIPpuMIsource720740760780800820840860
    taatttctttctcgataagaggccacgtgctttatgagggtaacatcaattcaagaaggagggaaacacttcctttttctggccctgataatagtatgagggtgaagccaaaataaaggattcgcgcccaaatcggcatctttaaatgcaggtatgcgatagttcctcactctttattaaagaaagagctattctccggtgcacgaaatactcccattgtagttaagttcttcctccctttgtgaaggaaaaagaccgggactattatcatactcccacttcggttttatttcctaagcgcgggtttagccgtagaaatttacgtccatacgctatcaaggagtgagaaaPmlIBfuAIBsaAIBspMIsource8809009209409609801,0001,0201,040
    ccttactcacgagtaattcttgcaaatgcctattatgcagatgttataatatctgtgcgtcttgagttgaagtcaggaatctaaaataaaaattaaggttaataaaaagaggaaagaaaaaaaaattaatcgatttacagaaacttgcacactaaaaatacacaactaaaagcaaggaatgagtgctcattaagaacgtttacggataatacgtctacaatattatagacacgcagaactcaacttcagtccttagattttatttttaattccaattatttttctcctttctttttttttaattagctaaatgtctttgaacgtgtgatttttatgtgttgattttcgttPsiIsource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttacagtatgggaagtcatcgacgttatctctactatagtatattatcatttctattattatcctgctcagtggtacttgcaaaacaagataagaccccattctttgaaggtacttccaggccggccgcacacaccatagcttcaaaatgtttctactccttttttactcttccaaatgtcatacccttcagtagctgcaatagagatgatatcatataatagtaaagataataataggacgagtcaccatgaacgttttgttctattctggggtaagaaacttccatgaaggtccggccggcgtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtEagIFseINaeINgoMIVTEF1 promotersource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctctggcggagcaaagaaaaagaagcagctttttccgttatttttaaaBplIBplIBsaIBtgZITEF1 promotersource1,4201,4401,4601,4801,5001,5201,5401,560
    ttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaataatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcaattatttgccagaagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttaTEF1 promotersource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctaatctaagttttaattacaaggatccgtaatacgactcactatagggcccgggcgtcgacatggaacagaagttgatttccgaagaagacctcgagtaagcttggtaccgcggctagctaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtcccgattagattcaaaattaatgttcctaggcattatgctgagtgatatcccgggcccgcagctgtaccttgtcttcaactaaaggcttcttctggagctcattcgaaccatggcgccgatcgattctaggcgagattggcttttccttcctcaatctgttggacttcagatccagggApaIBanIBmtIBtgIKpnINheISalISmaISrfITliIXhoIXmaIBamHIBsaHIPpuMIPspXISacIITspMIAcc65IHincIIPaeR7IPspOMIHindIIICYC1 terminatorMycT7T7 promoterTE...rsource1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaacgcgcggggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttaaaaagaaaaaaaagacatgtctgcgcacatgcgtacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttctaggtcgacgtaattacttagccggttgcgcgccccMluIBsrGIPvuIICYC1 terminatorsource1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccatctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtPciISapIBsaXIBspQIL4440source2,1202,1402,1602,1802,2002,2202,2402,260
    ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaDrdIoripBR322ori-Fsource2,2802,3002,3202,3402,3602,3802,4002,4202,440
    taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgApaLIBseYIorisource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcAlwNIorisource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaaBspHIorisource2,8202,8402,8602,8802,9002,9202,9402,960
    atcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagtagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatcAhdIBanIAmpRsource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggtattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccBglIBmrIBpmIBsaINmeAIIIAmpRsource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccAmpRsource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccgaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggcBcgIScaIAmpRsource3,5203,5403,5603,5803,6003,6203,6403,660
    gcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttcgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaaApaLIBsaHIAmpRAmp-Rsource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtgacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaBspHIAmpR promoterAmpRsource3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tccgcgcacatttccccgaaaagtgccacctgaacgaagcatctgtgcttcattttgtagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcaggcgcgtgtaaaggggcttttcacggtggacttgcttcgtagacacgaagtaaaacatcttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagactcgacgtaaaaatgtcttgtctttacgttgcgctttcgcgataaaatggttgcttcttagacacgaag2u orisource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    atttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagtaaaaacattttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagactcgacgtaaaaatgtcttgtctttacgttgcgctctcgcgataaaatggttgtttcttagatatgaagaaaaaacaagatgtttttacgtagggctctcgcgataaaaagattgtttc2u orisource4,2204,2404,2604,2804,3004,3204,3404,360
    catcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcggggtagaatctaatgaaaaaaagaggaaacacgcgagatattacgtcagagaactattgaaaaacgtgacatccaggcaattccaatcttcttccgatgaaaccacagataaaagagaaggtatttttttcggactgaggtgaagggcgcaaatgactaatgatcgcttcgacgcccAjuI2u orisource4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtaacgtaaaaaagttctatttccgtaggggctaatataagatatggctacacctaacgcgtatgaaacacttgtctttcactatcgcaactactaagaagtaaccagtcttttaatacttgccaaagaagataaaacagagatatatgatgcatatcctttacaaatgtaaaagcatBsaAISnaBI2u orisource4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacaacaaaagctaagtgagatacttatcaagaatgatgttaaaaaaacagatttctcattatgatctctatttgtattttttacatctccagctcaaatctacgttcaagttcctcgctttccacctacccatccaatatatccctatatcgtgtctctatatatcgtttctctatgCspCI2u orisource4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtccggtgcgtttttggttttttgaaagtgcgtcttcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagagaataggaacttcggaataggaacttcaaagcgtaaaactcgttacaaacaccttcgccataagcgttataaaatcatcgagcaatgtcaggccacgcaaaaaccaaaaaactttcacgcagaagtctcgcgaaaaccaaaagttttcgcgagacttcaaggatatgaaagatctcttatccttgaagccttatccttgaagtttcgcaBarIXbaI2u oriFRTsource4,9204,9404,9604,9805,0005,0205,0405,060
    ttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctcactgttcacgtcgcacctatatctgcgtgttgcctgtatatatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaagaaggcttttgctcgcgaaggcttttacgttgcgctcgacgcgtgtatgtcgagtgacaagtgcagcgtggatatagacgcacaacggacatatatatatatgtactcttcttgccgtatcacgcacaaatacgaatttacgcatgaatatacgcagataaatacatcctactttcBmgBI2u orisource5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgcttccttcagcactaccctttagctgttctatatgctgccactcctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcatggtagacaacccttaatataactcatcagatcatggaggacactataatagggtaaggtacgccccatagcatacgaaggaagtcgtgatgggaaatcgacaagatatacgacggtgaggagttaacctaatcagagtaggaagttacgatagtaaaggaaactataacctagtaccatctgttgggaattatattgaBarIMfeIXcmIBseRI2u orisource5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatagcatattacatacgatatgcttcaataatccagatctctagacaaatcgaacggagcaggggcggcccagtgggccggtcgctgtacctccgggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatcgggtatgtaXbaIBglIIBmgBIBpu10IBstEIITEF promoterkanMXloxPsource5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcgtttttaatgccgaggagcgacgtctggacgctcgtccctttgcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaaacggtgactMluIPstIBfuAIBseRIBspMITEF promoterkanMXsource5,6205,6405,6605,6805,7005,7205,7405,760
    ggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaaccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgtagtgtaggcttgtatttgttggtacccattccttttctgagtgcaaagctccggcgctaatttaaggttgtacctacgactaaatatacccatatttacccgagcgctattacagcccgttBtgINcoINruIStyIkanMXKanRTEF promoterKan-Rsource5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgagtccacgctgttagatagctaacatacccttcgggctacgcggtctcaacaaagactttgtaccgtttccatcgcaacggttactacaatgtctactctaccagtctgatttgaccgactgccttaaatacggagaaggctggtagttcgtaaaataggcatgaggactactackanMXKanRsource5,9605,9806,0006,0206,0406,0606,0806,1006,120
    catggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgtaccaatgagtggtgacgctaggggccgttttgtcgtaaggtccataatcttcttataggactaagtccacttttataacaactacgcgaccgtcacaaggacgcggccaacgtaagctaaggacaaacattaacaggaaaattgtcgctagcgcataaagcagagcgagtccgAsiSIBsmBIEcoNIEsp3IBpu10IkanMXKanRsource6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaacgttagtgcttacttattgccaaaccaactacgctcactaaaactactgctcgcattaccgaccggacaacttgttcagacctttctttacgtattcgaaaacggtaagagtggcctaagtcagcagtgagtaccactaaagagtgaactattggaataaaaactgctcccctttAjuIHindIIIkanMXKanRsource6,3206,3406,3606,3806,4006,4206,4406,460
    ttaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgaattatccaacataactacaacctgctcagccttagcgtctggctatggtcctagaacggtaggataccttgacggagccactcaaaagaggaagtaatgtctttgccgaaaaagtttttataccataactattaggactatacttatttaacgtcaaagtaaactacgagctacPflMIkanMXKanRsource6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcaaaaagattagtcatgactgttatttttctaagaacaaaagttcttgaacagtaaacatatcaaaaaaatataacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatScaIkanMXTEF terminatorKanRsource6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttattaggtgatatcagatccactaagtacgcagttagcatacacttacgaccagcgatatgacgacagctaagctatgattgcggcggtaggtcacagcttttgctcgagagctcttgggaattatattgaagcatattacatacgatatgcttcaataatccactatagtctaggtgatSacITliIXhoIEcoRVPaeR7IEco53kIkanMXTEF terminatorloxPsource6,8406,8606,8806,9006,9206,9406,9606,980
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    6981 bp5000550060006500500100015002u oriTEF1 promoterkanMXKan-RFLAGpHybLex-Rsource

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    6981