Welcome to Benchling

Benchling is an intelligent research platform with tools for note-taking, molecular biology, and sample tracking.

Create an account

Email
Already have an account?
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pCEV-G2-Km with αMF and ovalbumin
  • Sequence Map
    Editing disabled on read-only sequence.
    tcgacatggaacagaagttgatttccgaagaagacctcgagtaagcttggtaccgcggctagctaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaattttagctgtaccttgtcttcaactaaaggcttcttctggagctcattcgaaccatggcgccgatcgattctaggcgagattggcttttccttcctcaatctgttggacttcagatccagggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttaaaaBanIBmtIBtgIKpnINheIPpuMIPspXISacIIAcc65ICYC1 terminatorMycsource20406080100120140160
    tcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgcagaaaaaaaagacatgtctgcgcacatgcgtacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttctaggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgMluISapIBsaXIBspQIBsrGIPvuIICYC1 terminatorL4440source180200220240260280300320340
    tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgcPciIorisource360380400420440460480500520
    agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagDrdIoripBR322ori-Fsource540560580600620640660680700
    acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagatgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctAlwNIApaLIBseYIorisource720740760780800820840860
    gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaaorisource8809009209409609801,0001,0201,040
    gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttaBspHIorisource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctAhdIBanIBmrIBsaIAmpRsource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatBglINmeAIIIAmpRsource1,4201,4401,4601,4801,5001,5201,5401,560
    caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgAmpRsource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    agcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatttcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaBcgIScaIBsaHIAmpRAmp-Rsource1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgApaLIAmpRsource1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaacgaagcatctgtgcttcattttgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacttgcttcgtagacacgaagtaaaacBspHIAmpR promoter2u oriAmpRsource2,1202,1402,1602,1802,2002,2202,2402,260
    tagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgatcttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagactcgacgtaaaaatgtcttgtctttacgttgcgctttcgcgataaaatggttgcttcttagacacgaagtaaaaacattttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagac2u orisource2,2802,3002,3202,3402,3602,3802,4002,4202,440
    agctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactcgacgtaaaaatgtcttgtctttacgttgcgctctcgcgataaaatggttgtttcttagatatgaagaaaaaacaagatgtttttacgtagggctctcgcgataaaaagattgtttcgtagaatctaatgaaaaaaagaggaaacacgcgagatattacgtcagagaactattg2u orisource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgaaaaacgtgacatccaggcaattccaatcttcttccgatgaaaccacagataaaagagaaggtatttttttcggactgaggtgaagggcgcaaatgactaatgatcgcttcgacgcccacgtaaaaaagttctatttccgtaggggctaatataagatatggctacacctaacgcAjuI2u orisource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    catactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtagtatgaaacacttgtctttcactatcgcaactactaagaagtaaccagtcttttaatacttgccaaagaagataaaacagagatatatgatgcatatcctttacaaatgtaaaagcataacaaaagctaagtgagatacttatcaagaatgatgttaaaaaaacagatttctcatBsaAISnaBI2u orisource2,8202,8402,8602,8802,9002,9202,9402,960
    atactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtctatgatctctatttgtattttttacatctccagctcaaatctacgttcaagttcctcgctttccacctacccatccaatatatccctatatcgtgtctctatatatcgtttctctatgaaaactcgttacaaacaccttcgccataagcgttataaaatcatcgagcaatgtcagCspCI2u orisource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cggtgcgtttttggttttttgaaagtgcgtcttcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagagaataggaacttcggaataggaacttcaaagcgtttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctcactggccacgcaaaaaccaaaaaactttcacgcagaagtctcgcgaaaaccaaaagttttcgcgagacttcaaggatatgaaagatctcttatccttgaagccttatccttgaagtttcgcaaaggcttttgctcgcgaaggcttttacgttgcgctcgacgcgtgtatgtcgagtgacBarIXbaI2u oriFRTsource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttcacgtcgcacctatatctgcgtgttgcctgtatatatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaaggtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgcttccaagtgcagcgtggatatagacgcacaacggacatatatatatatgtactcttcttgccgtatcacgcacaaatacgaatttacgcatgaatatacgcagataaatacatcctactttccatcagatcatggaggacactataatagggtaaggtacgccccatagcatacgaaggBmgBI2u orisource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttcagcactaccctttagctgttctatatgctgccactcctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcatggtagacaacccttaatataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcaagtcgtgatgggaaatcgacaagatatacgacggtgaggagttaacctaatcagagtaggaagttacgatagtaaaggaaactataacctagtaccatctgttgggaattatattgaagcatattacatacgatatgcttcaataatccagatctctagacaaatcgaacggagBarIMfeIXbaIXcmIBglIIBseRI2u oriloxPsource3,5203,5403,5603,5803,6003,6203,6403,660
    gtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaacaggggcggcccagtgggccggtcgctgtacctccgggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatcgggtatgtaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcgtttBmgBIBpu10IBstEIITEF promoterkanMXsource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcattaatgccgaggagcgacgtctggacgctcgtccctttgcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaaacggtgactccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgtagtMluIPstIBfuAIBseRIBspMITEF promoterkanMXsource3,8603,8803,9003,9203,9403,9603,9804,0004,020
    catccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctggtaggcttgtatttgttggtacccattccttttctgagtgcaaagctccggcgctaatttaaggttgtacctacgactaaatatacccatatttacccgagcgctattacagcccgttagtccacgctgttagatagctaacatacccttcgggctacgcggtctcaacaaagacBtgINcoINruIStyIkanMXKanRKan-RTEF promotersource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaattttgtaccgtttccatcgcaacggttactacaatgtctactctaccagtctgatttgaccgactgccttaaatacggagaaggctggtagttcgtaaaataggcatgaggactactacgtaccaatgagtggtgacgctaggggccgttttgtcgtaaggtccataatcttcttakanMXKanRsource4,2204,2404,2604,2804,3004,3204,3404,360
    atcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaataggactaagtccacttttataacaactacgcgaccgtcacaaggacgcggccaacgtaagctaaggacaaacattaacaggaaaattgtcgctagcgcataaagcagagcgagtccgcgttagtgcttacttattgccaaaccaactacgctcactaaaactactgctcgcattAjuIAsiSIBsmBIEcoNIEsp3IBpu10IkanMXKanRsource4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttaccgaccggacaacttgttcagacctttctttacgtattcgaaaacggtaagagtggcctaagtcagcagtgagtaccactaaagagtgaactattggaataaaaactgctcccctttaattatccaacataactacaacctgctcagccttagcgtctggctatggtcctagaakanMXKanRsource4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcattcggtaggataccttgacggagccactcaaaagaggaagtaatgtctttgccgaaaaagtttttataccataactattaggactatacttatttaacgtcaaagtaaactacgagctactcaaaaagattagtcatgactgttatttttctaagaacaaaagttcttgaacagtaaScaIPflMIkanMXKanRTEF terminatorsource4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaaacatatcaaaaaaatataacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatagtacgcagttagcatacacttacgaccagcgatatgacgacagctaagctatgattkanMXTEF terminatorsource4,9204,9404,9604,9805,0005,0205,0405,060
    cgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttattaggtgatatcagatccactaggagcgacctcatgctatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttgcggcggtaggtcacagcttttgctcgagagctcttgggaattatattgaagcatattacatacgatatgcttcaataatccactatagtctaggtgatcctcgctggagtacgatatggactctttcgttggactggatgtcctttctcaatgagttcttattcttaaaagcaaSacIEcoRVEco53kIADH1 terminatorloxPsourcesource5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcttatttagaagtgtcaacaacgtatctaccaacgatttgacccttttccatcttttcgtaaatttctggcaaggtagacaagccaattttggattctcagtgaaattttaaacatatgtgaataaaaaaaatattgaataaattattatttttagtatttagtattctttaagcgaataaatcttcacagttgttgcatagatggttgctaaactgggaaaaggtagaaaagcatttaaagaccgttccatctgttcggBstZ17IADH1 terminatorpHybLex-Rsource5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gacaaccttgattggagacttgaccaaacctctggcgaagaattgttaattaagagctcagatcttatcgtcgtcatccttgtaatccatcgatactagtgcggccgcttgttttatatttgttgtaaaaagtagataattacttccttgatgatctgtaaaaaagagaaaaagactgttggaactaacctctgaactggtttggagaccgcttcttaacaattaattctcgagtctagaatagcagcagtaggaacattaggtagctatgatcacgccggcgaacaaaatataaacaacatttttcatctattaatgaaggaactactagacattttttctctttttctBcgIEagINotIPacISacISpeIBglIIEco53kIFLAGsource5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aagcatctaagaacttgaaaaactacgaattagaaaagaccaaatatgtatttcttgcattgaccaatttatgcaagtttatatatatgtaaatgtaagtttcacgaggttctactaaactaaaccacccccttggttagaagaaaagagtgtgtgagaacaggctgttgttgtcttcgtagattcttgaactttttgatgcttaatcttttctggtttatacataaagaacgtaactggttaaatacgttcaaatatatatacatttacattcaaagtgctccaagatgatttgatttggtgggggaaccaatcttcttttctcacacactcttgtccgacaacaacagStyIsource5,6205,6405,6605,6805,7005,7205,7405,760
    acacgattcggacaattctgtttgaaagagagagagtaacagtacgatcgaacgaactttgctctggagatcacagtgggcatcatagcatgtggtactaaaccctttcccgccattccagaaccttcgattgcttgttacaaaacctgtgagccgtcgctaggaccttgttgtgtgtgctaagcctgttaagacaaactttctctctctcattgtcatgctagcttgcttgaaacgagacctctagtgtcacccgtagtatcgtacaccatgatttgggaaagggcggtaaggtcttggaagctaacgaacaatgttttggacactcggcagcgatcctggaacaacacPpuMIsource5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgacgaaattggaagctgcaatcaataggaagacaggaagtcgagcgtgtctgggttttttcagttttgttctttttgcaaacaaatcacgagcgacggtaatttctttctcgataagaggccacgtgctttatgagggtaacatcaattcaagaaggagggaaacacttcctttactgctttaaccttcgacgttagttatccttctgtccttcagctcgcacagacccaaaaaagtcaaaacaagaaaaacgtttgtttagtgctcgctgccattaaagaaagagctattctccggtgcacgaaatactcccattgtagttaagttcttcctccctttgtgaaggaaaAhdIPmlIBsaAIsource5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttctggccctgataatagtatgagggtgaagccaaaataaaggattcgcgcccaaatcggcatctttaaatgcaggtatgcgatagttcctcactctttccttactcacgagtaattcttgcaaatgcctattatgcagatgttataatatctgtgcgtcttgagttgaagtcagaagaccgggactattatcatactcccacttcggttttatttcctaagcgcgggtttagccgtagaaatttacgtccatacgctatcaaggagtgagaaaggaatgagtgctcattaagaacgtttacggataatacgtctacaatattatagacacgcagaactcaacttcagtcBfuAIBspMIsource6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gaatctaaaataaaaattaaggttaataaaaagaggaaagaaaaaaaaattaatcgatttacagaaacttgcacactaaaaatacacaactaaaagcaattacagtatgggaagtcatcgacgttatctctactatagtatattatcatttctattattatcctgctcagtggtacttagattttatttttaattccaattatttttctcctttctttttttttaattagctaaatgtctttgaacgtgtgatttttatgtgttgattttcgttaatgtcatacccttcagtagctgcaatagagatgatatcatataatagtaaagataataataggacgagtcaccatsource6,3206,3406,3606,3806,4006,4206,4406,460
    cttgcaaaacaagataagaccccattctttgaaggtacttccaggccggccgcacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttctgaacgttttgttctattctggggtaagaaacttccatgaaggtccggccggcgtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaEagIFseINaeIBtgZINgoMIVTEF1 promotersource6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctctggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcBplIBplIBsaITEF1 promotersource6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaggatccgtaatacgactcactatagggcccgggcgtcgaatgagatttccttcaaattatttgccagaagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatgttcctaggcattatgctgagtgatatcccgggcccgcagcttactctaaaggaagtApaISmaISrfIXmaIBamHIBsaHITspMIPspOMITEF1 promoterT7T7 promoter-alp...nalsourcesource6,8406,8606,8806,9006,9206,9406,9606,9807,000
    atttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaaggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccaataBstAPI-alpha-factor secretion signalsourcesource7,0207,0407,0607,0807,1007,1207,1407,160
    tgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaatacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgatactctaaaggaagttaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaBstAPI-alpha-factor secretion signalsourcesource-alpha-factor secretion signalsource7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctaggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccaataacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgasource-alpha-factor secretion signalsource7,3607,3807,4007,4207,4407,4607,4807,500
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Assembly Parameters
    • Linear Map
    Editing disabled on read-only sequence.
    7519 bp550060006500700050010001500sourceAmpRpBR322ori-FpPTK005-3a-αMF REV (1) (DNA)pPTK005-3a-αMF REV (2) (DNA)

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7519