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pCEV-G2-Km with AMF and Ovalbumin
  • Sequence Map
    Editing disabled on read-only sequence.
    tcgacatggaacagaagttgatttccgaagaagacctcgagtaagcttggtaccgcggctagctaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaattttagctgtaccttgtcttcaactaaaggcttcttctggagctcattcgaaccatggcgccgatcgattctaggcgagattggcttttccttcctcaatctgttggacttcagatccagggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttaaaaBmtIKpnINheISalIPpuMIPspXISacIIAcc65ICYC1 terminatorMycsource20406080100120140160
    tcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgcagaaaaaaaagacatgtctgcgcacatgcgtacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttctaggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgMluISapIBsaXIBspQIBsrGIPvuIICYC1 terminatorL4440source180200220240260280300320340
    tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgcPciIorisource360380400420440460480500520
    agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagDrdIoripBR322ori-Fsource540560580600620640660680700
    acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagatgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctAlwNIApaLIBseYIorisource720740760780800820840860
    gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaaorisource8809009209409609801,0001,0201,040
    gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttaBspHIorisource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctAhdIBmrIBsaIAmpRsource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatBglINmeAIIIAmpRsource1,4201,4401,4601,4801,5001,5201,5401,560
    caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgAmpRsource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    agcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatttcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaBcgIScaIAmpRAmp-Rsource1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgApaLIAmpRsource1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaacgaagcatctgtgcttcattttgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacttgcttcgtagacacgaagtaaaacBspHIAmpR promoter2u oriAmpRsource2,1202,1402,1602,1802,2002,2202,2402,260
    tagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgatcttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagactcgacgtaaaaatgtcttgtctttacgttgcgctttcgcgataaaatggttgcttcttagacacgaagtaaaaacattttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagac2u orisource2,2802,3002,3202,3402,3602,3802,4002,4202,440
    agctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactcgacgtaaaaatgtcttgtctttacgttgcgctctcgcgataaaatggttgtttcttagatatgaagaaaaaacaagatgtttttacgtagggctctcgcgataaaaagattgtttcgtagaatctaatgaaaaaaagaggaaacacgcgagatattacgtcagagaactattg2u orisource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgaaaaacgtgacatccaggcaattccaatcttcttccgatgaaaccacagataaaagagaaggtatttttttcggactgaggtgaagggcgcaaatgactaatgatcgcttcgacgcccacgtaaaaaagttctatttccgtaggggctaatataagatatggctacacctaacgcAjuI2u orisource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    catactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtagtatgaaacacttgtctttcactatcgcaactactaagaagtaaccagtcttttaatacttgccaaagaagataaaacagagatatatgatgcatatcctttacaaatgtaaaagcataacaaaagctaagtgagatacttatcaagaatgatgttaaaaaaacagatttctcatBsaAISnaBI2u orisource2,8202,8402,8602,8802,9002,9202,9402,960
    atactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtctatgatctctatttgtattttttacatctccagctcaaatctacgttcaagttcctcgctttccacctacccatccaatatatccctatatcgtgtctctatatatcgtttctctatgaaaactcgttacaaacaccttcgccataagcgttataaaatcatcgagcaatgtcagCspCI2u orisource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cggtgcgtttttggttttttgaaagtgcgtcttcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagagaataggaacttcggaataggaacttcaaagcgtttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctcactggccacgcaaaaaccaaaaaactttcacgcagaagtctcgcgaaaaccaaaagttttcgcgagacttcaaggatatgaaagatctcttatccttgaagccttatccttgaagtttcgcaaaggcttttgctcgcgaaggcttttacgttgcgctcgacgcgtgtatgtcgagtgacBarIXbaI2u oriFRTsource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttcacgtcgcacctatatctgcgtgttgcctgtatatatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaaggtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgcttccaagtgcagcgtggatatagacgcacaacggacatatatatatatgtactcttcttgccgtatcacgcacaaatacgaatttacgcatgaatatacgcagataaatacatcctactttccatcagatcatggaggacactataatagggtaaggtacgccccatagcatacgaaggBmgBI2u orisource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttcagcactaccctttagctgttctatatgctgccactcctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcatggtagacaacccttaatataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgcctcaagtcgtgatgggaaatcgacaagatatacgacggtgaggagttaacctaatcagagtaggaagttacgatagtaaaggaaactataacctagtaccatctgttgggaattatattgaagcatattacatacgatatgcttcaataatccagatctctagacaaatcgaacggagBarIMfeIXbaIXcmIBglII2u oriloxPsource3,5203,5403,5603,5803,6003,6203,6403,660
    gtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaacaggggcggcccagtgggccggtcgctgtacctccgggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatcgggtatgtaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcgtttBmgBIBstEIITEF promoterkanMXsource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcattaatgccgaggagcgacgtctggacgctcgtccctttgcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaaacggtgactccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgtagtMluIPstIBfuAIBspMITEF promoterkanMXsource3,8603,8803,9003,9203,9403,9603,9804,0004,020
    catccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctggtaggcttgtatttgttggtacccattccttttctgagtgcaaagctccggcgctaatttaaggttgtacctacgactaaatatacccatatttacccgagcgctattacagcccgttagtccacgctgttagatagctaacatacccttcgggctacgcggtctcaacaaagacNruIkanMXKanRKan-RTEF promotersource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaattttgtaccgtttccatcgcaacggttactacaatgtctactctaccagtctgatttgaccgactgccttaaatacggagaaggctggtagttcgtaaaataggcatgaggactactacgtaccaatgagtggtgacgctaggggccgttttgtcgtaaggtccataatcttcttakanMXKanRsource4,2204,2404,2604,2804,3004,3204,3404,360
    atcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaataggactaagtccacttttataacaactacgcgaccgtcacaaggacgcggccaacgtaagctaaggacaaacattaacaggaaaattgtcgctagcgcataaagcagagcgagtccgcgttagtgcttacttattgccaaaccaactacgctcactaaaactactgctcgcattAjuIAsiSIBsmBIEcoNIEsp3IkanMXKanRsource4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttaccgaccggacaacttgttcagacctttctttacgtattcgaaaacggtaagagtggcctaagtcagcagtgagtaccactaaagagtgaactattggaataaaaactgctcccctttaattatccaacataactacaacctgctcagccttagcgtctggctatggtcctagaakanMXKanRsource4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcattcggtaggataccttgacggagccactcaaaagaggaagtaatgtctttgccgaaaaagtttttataccataactattaggactatacttatttaacgtcaaagtaaactacgagctactcaaaaagattagtcatgactgttatttttctaagaacaaaagttcttgaacagtaaScaIPflMIkanMXKanRTEF terminatorsource4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaaacatatcaaaaaaatataacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatagtacgcagttagcatacacttacgaccagcgatatgacgacagctaagctatgattkanMXTEF terminatorsource4,9204,9404,9604,9805,0005,0205,0405,060
    cgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttattaggtgatatcagatccactaggagcgacctcatgctatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttgcggcggtaggtcacagcttttgctcgagagctcttgggaattatattgaagcatattacatacgatatgcttcaataatccactatagtctaggtgatcctcgctggagtacgatatggactctttcgttggactggatgtcctttctcaatgagttcttattcttaaaagcaaSacIEcoRVEco53kIADH1 terminatorloxPsourcesource5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcttatttagaagtgtcaacaacgtatctaccaacgatttgacccttttccatcttttcgtaaatttctggcaaggtagacaagccaattttggattctcagtgaaattttaaacatatgtgaataaaaaaaatattgaataaattattatttttagtatttagtattctttaagcgaataaatcttcacagttgttgcatagatggttgctaaactgggaaaaggtagaaaagcatttaaagaccgttccatctgttcggBstZ17IADH1 terminatorpHybLex-Rsource5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gacaaccttgattggagacttgaccaaacctctggcgaagaattgttaattaagagctcagatcttatcgtcgtcatccttgtaatccatcgatactagtgcggccgcttgttttatatttgttgtaaaaagtagataattacttccttgatgatctgtaaaaaagagaaaaagactgttggaactaacctctgaactggtttggagaccgcttcttaacaattaattctcgagtctagaatagcagcagtaggaacattaggtagctatgatcacgccggcgaacaaaatataaacaacatttttcatctattaatgaaggaactactagacattttttctctttttctBcgIEagINotIPacISacISpeIBglIIEco53kIFLAGsource5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aagcatctaagaacttgaaaaactacgaattagaaaagaccaaatatgtatttcttgcattgaccaatttatgcaagtttatatatatgtaaatgtaagtttcacgaggttctactaaactaaaccacccccttggttagaagaaaagagtgtgtgagaacaggctgttgttgtcttcgtagattcttgaactttttgatgcttaatcttttctggtttatacataaagaacgtaactggttaaatacgttcaaatatatatacatttacattcaaagtgctccaagatgatttgatttggtgggggaaccaatcttcttttctcacacactcttgtccgacaacaacagsource5,6205,6405,6605,6805,7005,7205,7405,760
    acacgattcggacaattctgtttgaaagagagagagtaacagtacgatcgaacgaactttgctctggagatcacagtgggcatcatagcatgtggtactaaaccctttcccgccattccagaaccttcgattgcttgttacaaaacctgtgagccgtcgctaggaccttgttgtgtgtgctaagcctgttaagacaaactttctctctctcattgtcatgctagcttgcttgaaacgagacctctagtgtcacccgtagtatcgtacaccatgatttgggaaagggcggtaaggtcttggaagctaacgaacaatgttttggacactcggcagcgatcctggaacaacacPpuMIsource5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgacgaaattggaagctgcaatcaataggaagacaggaagtcgagcgtgtctgggttttttcagttttgttctttttgcaaacaaatcacgagcgacggtaatttctttctcgataagaggccacgtgctttatgagggtaacatcaattcaagaaggagggaaacacttcctttactgctttaaccttcgacgttagttatccttctgtccttcagctcgcacagacccaaaaaagtcaaaacaagaaaaacgtttgtttagtgctcgctgccattaaagaaagagctattctccggtgcacgaaatactcccattgtagttaagttcttcctccctttgtgaaggaaaAhdIPmlIBsaAIsource5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttctggccctgataatagtatgagggtgaagccaaaataaaggattcgcgcccaaatcggcatctttaaatgcaggtatgcgatagttcctcactctttccttactcacgagtaattcttgcaaatgcctattatgcagatgttataatatctgtgcgtcttgagttgaagtcagaagaccgggactattatcatactcccacttcggttttatttcctaagcgcgggtttagccgtagaaatttacgtccatacgctatcaaggagtgagaaaggaatgagtgctcattaagaacgtttacggataatacgtctacaatattatagacacgcagaactcaacttcagtcBfuAIBspMIsource6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gaatctaaaataaaaattaaggttaataaaaagaggaaagaaaaaaaaattaatcgatttacagaaacttgcacactaaaaatacacaactaaaagcaattacagtatgggaagtcatcgacgttatctctactatagtatattatcatttctattattatcctgctcagtggtacttagattttatttttaattccaattatttttctcctttctttttttttaattagctaaatgtctttgaacgtgtgatttttatgtgttgattttcgttaatgtcatacccttcagtagctgcaatagagatgatatcatataatagtaaagataataataggacgagtcaccatsource6,3206,3406,3606,3806,4006,4206,4406,460
    cttgcaaaacaagataagaccccattctttgaaggtacttccaggccggccgcacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttctgaacgttttgttctattctggggtaagaaacttccatgaaggtccggccggcgtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaEagIFseINaeIBtgZINgoMIVTEF1 promotersource6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctctggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcBplIBplIBsaITEF1 promotersource6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaggatccgtaatacgactcactatagggcccgggcgtcgaatgagatttccttcaaattatttgccagaagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatgttcctaggcattatgctgagtgatatcccgggcccgcagcttactctaaaggaagtApaISmaISrfIXmaIBamHITspMIPspOMITEF1 promoterT7T7 promoter-alp...nalsourcesource6,8406,8606,8806,9006,9206,9406,9606,9807,000
    atttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaaggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccaataBstAPI-alpha-factor secretion signalsourcesource7,0207,0407,0607,0807,1007,1207,1407,160
    tgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctATGGGTTCTATAGGTGCCGCCTCTATGGAATTTTGTTTCGATGTATTCAAGGAGTTAAAGGTTCACCACGCAAATGAAAATATATTTTATTGCCCAATacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgaTACCCAAGATATCCACGGCGGAGATACCTTAAAACAAAGCTACATAAGTTCCTCAATTTCCAAGTGGTGCGTTTACTTTTATATAAAATAACGGGTTA-alpha-factor secretion signalsourcesource7,1807,2007,2207,2407,2607,2807,3007,3207,340
    AGCAATCATGTCAGCCCTGGCGATGGTTTACTTAGGTGCAAAAGATTCAACACGAACACAGATTAACAAGGTTGTAAGATTCGACAAACTGCCAGGTTTTGGGGATAGCATAGAAGCTCAGTGCGGTACATCAGTTAACGTCCATAGTAGTCTAAGAGACATTTTGAATCAGATCTCGTTAGTACAGTCGGGACCGCTACCAAATGAATCCACGTTTTCTAAGTTGTGCTTGTGTCTAATTGTTCCAACATTCTAAGCTGTTTGACGGTCCAAAACCCCTATCGTATCTTCGAGTCACGCCATGTAGTCAATTGCAGGTATCATCAGATTCTCTGTAAAACTTAGTCTAGBaeIHpaIBtgZIPflMIsource7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ACCAAGCCTAACGACGTCTATTCCTTTAGCTTAGCGAGCAGACTATACGCTGAGGAACGGTACCCCATTTTGCCTGAATATCTACAATGTGTTAAAGAGTTGTATAGAGGTGGCTTGGAACCGATTAATTTCCAAACTGCTGCAGATCAAGCTAGGGAATTAATTAACTCTTGGGTGGTTCGGATTGCTGCAGATAAGGAAATCGAATCGCTCGTCTGATATGCGACTCCTTGCCATGGGGTAAAACGGACTTATAGATGTTACACAATTTCTCAACATATCTCCACCGAACCTTGGCTAATTAAAGGTTTGACGACGTCTAGTTCGATCCCTTAATTAATTGAGAACCCBlpIKpnIPacIPstIZraIAatIIBbvCIAcc65Isource7,5407,5607,5807,6007,6207,6407,6607,6807,700
    TAGAATCCCAAACGAACGGTATCATAAGAAATGTTTTGCAACCTTCTTCAGTGGACTCTCAGACCGCCATGGTTCTCGTCAATGCTATCGTGTTCAAAGGACTCTGGGAAAAAGCATTTAAAGATGAAGATACGCAAGCAATGCCATTTAGGGTGACTGAGCAAGAGTCAAAACCATCTTAGGGTTTGCTTGCCATAGTATTCTTTACAAAACGTTGGAAGAAGTCACCTGAGAGTCTGGCGGTACCAAGAGCAGTTACGATAGCACAAGTTTCCTGAGACCCTTTTTCGTAAATTTCTACTTCTATGCGTTCGTTACGGTAAATCCCACTGACTCGTTCTCAGTTTTGGBstAPIsource7,7207,7407,7607,7807,8007,8207,8407,860
    AGTTCAAATGATGTACCAAATTGGACTTTTTAGAGTCGCGTCGATGGCCAGTGAAAAAATGAAGATATTAGAATTACCATTTGCTTCTGGAACAATGTCGATGTTAGTCCTGCTTCCAGATGAAGTGTCCGGCTTGGAACAGCTGGAGTCTATTATCAATTTTGAAAAGTTGACTTCAAGTTTACTACATGGTTTAACCTGAAAAATCTCAGCGCAGCTACCGGTCACTTTTTTACTTCTATAATCTTAATGGTAAACGAAGACCTTGTTACAGCTACAATCAGGACGAAGGTCTACTTCACAGGCCGAACCTTGTCGACCTCAGATAATAGTTAAAACTTTTCAACTGAMscIPvuIIsource7,8807,9007,9207,9407,9607,9808,0008,0208,040
    GAATGGACCAGTTCGAACGTTATGGAAGAAAGAAAAATTAAGGTATATCTACCTCGTATGAAAATGGAAGAAAAATATAATTTAACTAGTGTGCTTATGGCCATGGGAATTACAGATGTTTTCTCCTCATCCGCTAACTTATCTGGTATCTCTTCAGCCGAAAGCTTGAAGATAACTTACCTGGTCAAGCTTGCAATACCTTCTTTCTTTTTAATTCCATATAGATGGAGCATACTTTTACCTTCTTTTTATATTAAATTGATCACACGAATACCGGTACCCTTAATGTCTACAAAAGAGGAGTAGGCGATTGAATAGACCATAGAGAAGTCGGCTTTCGAACTTCTATTMscISpeIBstBIsource8,0608,0808,1008,1208,1408,1608,1808,2008,220
    GTCAAGCCGTACATGCGGCTCATGCTGAGATTAATGAGGCAGGGCGCGAGGTGGTCGGTTCCGCTGAGGCAGGCGTAGATGCAGCATCAGTTTCTGAAGAATTTAGGGCTGACCACCCCTTTCTTTTCTGTATTAAACATATCGCTACTAATGCTGTATTGTTCTTTGGCCGTTGCAGTTCGGCATGTACGCCGAGTACGACTCTAATTACTCCGTCCCGCGCTCCACCAGCCAAGGCGACTCCGTCCGCATCTACGTCGTAGTCAAAGACTTCTTAAATCCCGACTGGTGGGGAAAGAAAAGACATAATTTGTATAGCGATGATTACGACATAACAAGAAACCGGCAACAlwNIBbvCIsource8,2408,2608,2808,3008,3208,3408,3608,3808,400
    TGTTTCGCCGACAAAGCGGCso...e8,410
    • Plasmid
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    Editing disabled on read-only sequence.
    8410 bp60006500700075008000500100015002000sourceAmpRAmp-RsourcepPTK005-3a-αMF REV (DNA)OVAL_CHICK (codon optimized) REV (DNA)

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    8410