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876 - pGAD BD MDMX Full
  • Sequence Map
Editing disabled on read-only sequence.
tgcatgcctgcaggtcgagatccgggatcgaagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatacgtacggacgtccagctctaggccctagcttctttactaccatttactttatcctttagttcctcgtacttccgttttctgtttatattcccagcttgctttttatttcacttttcacaactatactacataaaccgaaacgccgcggcttttttgctcaaatgcgttaacgtgttaKasINarIPstISbfISfoIPluTIADH1\PromoterADH1 promoter20406080100120140160
catgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacagtacgactgagacaccgcctgggcgcgagaacggccgggccgctattgcgacccgcactccgacacgggccgcctcaaaaaacgcggacgtaaaaggttccaaatgggacgcgattccccgctctaacctcttcgttattcttacggccaaccccaacgctactactgctggtgctgtBglIBsmINaeIBpu10INgoMIVADH1\PromoterADH1 promoter180200220240260280300320340
actggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagctgaccacagtaataaattcaacggctttcttggactcacgtaaacgttgtactcatatgatcttcttactcggttctgaacgctctgcgctcaaacggccaccacgcttgttatctcgctggtactggaacttccactctgcgcgtattggcgatctcatgaaacttctcctttgtcgScaIADH1\PromoterADH1 promoter360380400420440460480500520
aatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggttatcccaacgatggtcatatttatctgtccatgtatgttgtgacctttaccaacagacaaactcatgcgaaagttaagtaaacccacacgtgaaataatacaatgttataccttcccttgaaatgtgaagaggatacgtgtatataattaatttcaggttacgatcatctcttccccPacIADH1\PromoterADH1 promoter540560580600620640660680700
ggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggaccattgtggggaggcgcgagaaaaggctaaaaaaagatttggcaccttataaagcctataggaaaacaacaaaggcccacatgttatacctgaaggagaaaagaccgttggtttgggtatgtagccctaaggatattatggaagcaaccagagggattgtacatccaccgcctcccctADH1\PromoterADH1 promoter720740760780800820840860880
gatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataactatatgttatcttgtctatggtctgttctgtattacccgatttgttctgatgtggttaatgtgacggagtaactaccaccatgtattgcttgattatgacatcgggatctgaactatcggtagtagtatagcttcaaagtgatgggaaaaaggtaaacggtagataacttcattattBsaBIADH1\PromoterADH1 promoter9009209409609801,0001,0201,0401,060
taggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaatccgcgtacgttgaagaaaagaaaaaaaaagaaaagagagagggggcaacaacagagtggtataggcgttactgtttttttactaccttctgtgatttccttttttaattgctgtttctgtcgtggttgtctacagcaacaaggtctcgactactccccatagagcttcgtgtgcttADH1\PromoterADH1 promoter1,0801,1001,1201,1401,1601,1801,2001,2201,240
actttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgaaaaaggaaggaagtaagtgcgtgtgatgagagattactcgttgccatatgccggaaggaaggtcaatgaacttaaactttattttttttcaaacgacagaacgatagttcatatttatctggacgttaataattagaaaacaaaggagcagtaacaagagcaagggaaagaaggaBsgIADH1\PromoterADH1 promoter1,2601,2801,3001,3201,3401,3601,3801,4001,420
tgtttctttttctgcacaatatttcaagctataccaagcatacaatcaactccaagctttgcaaagatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtacaaagaaaaagacgtgttataaagttcgatatggttcgtatgttagttgaggttcgaaacgtttctacttcgatgacagaagatagcttgttcgtacgctataaacggctgaatttttcgagttcacgaggtttctttttggcttcacgcggttcacagacttcttgttgaccctcaHindIIIMK2LL4SS6IE8QA10CD12IC14RL16KK18LK20CS22KE24KP26KC28AK30CL32KN34NW36E1491-3533GAL4\DNA\binding\domainGal4 DNA-binding domainADH1\PromoterADH1 promoter1,4401,4601,4801,5001,5201,5401,5601,5801,600
gtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattcagcgatgagagggttttggttttccagaggcgactgatcccgtgtagactgtcttcaccttagttccgatctttctgaccttgtcgataaagatgactaaaaaggagctcttctggaactgtactaaaacttttacctaagaaatgtcctatattttcgtaacaattgtcctaataaAvaIHpaITliIXhoIBsoBIPaeR7IC38RY40SP42KT44KR46SP48LT50RA52HL54TE56VE58SR60LE62RL64EQ66LF68LL70IF72PR74ED76LD78MI80LK82MD84SL86QD88IK90AL92LT94GL96F1491-3533GAL4\DNA\binding\domainGal4 DNA-binding domain1,6201,6401,6601,6801,7001,7201,7401,7601,780
tgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttgtaatacgactcactataacatgttctattacacttatttctacggcagtgtctatctaaccgaagtcacctctgactatacggagattgtaactctgtcgtatcttattcacgctgtagtagtagccttctctcatcattgtttccagtttctgtcaactgacatagcggccttaaacattatgctgagtgatatV98QD100NV102NK104DA106VT108DR110LA112SV114ET116DM118PL120TL122RQ124HR126IS128AT130SS132SE134ES136SN138KG140QR142QL144TV146SP148EF150VI152RL154TI1561491-3533GAL4\DNA\binding\domainGal4 DNA-binding domainT7T7\RNA...moter1,8001,8201,8401,8601,8801,9001,9201,940
gggcgagccgccatcatggaggagcagaagctgatctcagaggaggacctgcatatggccatggaggccgaattcATGACATCATTTTCCACCTCTGCTCAGTGTTCGACATCTGACAGTGCTTGCAGGATCTCTCCTGGACAAATCAATCAGGTACGACCAAAACTGCCGCTTTTGAcccgctcggcggtagtacctcctcgtcttcgactagagtctcctcctggacgtataccggtacctccggcttaagTACTGTAGTAAAAGGTGGAGACGAGTCACAAGCTGTAGACTGTCACGAACGTCCTAGAGAGGACCTGTTTAGTTAGTCCATGCTGGTTTTGACGGCGAAAACTBglINcoINdeISfiIEcoRIPpuMIGR158AA160IM162EE164QK166LI168SE170ED172LH174MA176ME178AE180FM182TS184FS186TS188AQ190CS192TS194DS196AC198RI200SP202GQ204IN206QV208RP210KL212PL214L1491-3533Full MDMX WTc-Myc\tagMCS1,9601,9802,0002,0202,0402,0602,0802,1002,120
AGATTTTGCATGCAGCAGGTGCGCAAGGTGAAATGTTCACTGTTAAAGAGGTCATGCACTATTTAGGTCAGTACATAATGGTGAAGCAACTTTATGATCAGCAGGAGCAGCATATGGTATATTGTGGTGGAGATCTTTTGGGAGAACTACTGGGACGTCAGAGCTTCTCCGTGAAAGATCTAAAACGTACGTCGTCCACGCGTTCCACTTTACAAGTGACAATTTCTCCAGTACGTGATAAATCCAGTCATGTATTACCACTTCGTTGAAATACTAGTCGTCCTCGTCGTATACCATATAACACCACCTCTAGAAAACCCTCTTGATGACCCTGCAGTCTCGAAGAGGCACTTTCTBclINdeIZraIAatIIBglIIPaqCIBstAPIK216IL218HA220AG222AQ224GE226MF228TV230KE232VM234HY236LG238QY240IM242VK244QL246YD248QQ250EQ252HM254VY256CG258GD260LL262GE264LL266GR268QS270FS272VK274D1491-3533Full MDMX WT2,1402,1602,1802,2002,2202,2402,2602,2802,300
CCCAAGCCCTCTCTATGATATGCTAAGAAAGAATCTTGTCACTTTAGCCACTGCTACTACAGATGCTGCTCAGACTCTCGCTCTCGCACAGGATCACAGTATGGATATTCCAAGTCGAGACCAACTGAAGCAAAGTGCAGAGGAAAGTTCCACTTCCAGAAAAAGAACTACAGAAGACGGGTTCGGGAGAGATACTATACGATTCTTTCTTAGAACAGTGAAATCGGTGACGATGATGTCTACGACGAGTCTGAGAGCGAGAGCGTGTCCTAGTGTCATACCTATAAGGTTCAGCTCTGGTTGACTTCGTTTCACGTCTCCTTTCAAGGTGAAGGTCTTTTTCTTGATGTCTTCTGBsgIP276SP278LY280DM282LR284KN286LV288TL290AT292AT294TD296AA298QT300LA302LA304QD306HS308MD310IP312SR314DQ316LK318QS320AE322ES324ST326SR328KR330TT332ED3341491-3533Full MDMX WT2,3202,3402,3602,3802,4002,4202,4402,4602,480
GATATCCCCACACTGCCTACCTCAGAGCATAAATGCATACATTCTAGAGAAGATGAAGACTTAATTGAAAATTTAGCCCAAGATGAAACATCTAGGCTGGACCTTGGATTTGAGGAGTGGGATGTAGCTGGCCTGCCTTGGTGGTTTTTAGGAAACTTGAGAAGCAACTATACACCTACTATAGGGGTGTGACGGATGGAGTCTCGTATTTACGTATGTAAGATCTCTTCTACTTCTGAATTAACTTTTAAATCGGGTTCTACTTTGTAGATCCGACCTGGAACCTAAACTCCTCACCCTACATCGACCGGACGGAACCACCAAAAATCCTTTGAACTCTTCGTTGATATGTGGATNsiIXbaIDI336PT338LP340TS342EH344KC346IH348SR350ED352ED354LI356EN358LA360QD362ET364SR366LD368LG370FE372EW374DV376AG378LP380WW382FL384GN386LR388SN390YT392P1491-3533Full MDMX WT2,5002,5202,5402,5602,5802,6002,6202,6402,660
GAAGTAATGGCTCAACTGATTTACAGACAAATCAGGATGTGGGTACTGCCATTGTTTCAGATACTACAGATGACTTGTGGTTTTTGAATGAGTCAGTATCAGAGCAGTTAGGTGTTGGAATAAAAGTTGAAGCTGCTGATACTGAACAAACAAGTGAAGAAGTAGGGAAAGTAAGTGACTTCATTACCGAGTTGACTAAATGTCTGTTTAGTCCTACACCCATGACGGTAACAAAGTCTATGATGTCTACTGAACACCAAAAACTTACTCAGTCATAGTCTCGTCAATCCACAACCTTATTTTCAACTTCGACGACTATGACTTGTTTGTTCACTTCTTCATCCCTTTCATTCACTR394SN396GS398TD400LQ402TN404QD406VG408TA410IV412SD414TT416DD418LW420FL422NE424SV426SE428QL430GV432GI434KV436EA438AD440TE442QT444SE446EV448GK450VS452D1491-3533Full MDMX WT2,6802,7002,7202,7402,7602,7802,8002,8202,840
CAAAAAGGTGATTGAAGTGGGAAAAAATGATGACCTGGAGGACTCTAAGTCCTTAAGTGATGATACCGATGTAGAGGTTACCTCTGAGGATGAGTGGCAGTGTACTGAATGCAAGAAATTTAACTCTCCAAGCAAGAGGTACTGTTTTCGTTGTTGGGCCTTGAGGAAGGATTGGTATGTTTTTCCACTAACTTCACCCTTTTTTACTACTGGACCTCCTGAGATTCAGGAATTCACTACTATGGCTACATCTCCAATGGAGACTCCTACTCACCGTCACATGACTTACGTTCTTTAAATTGAGAGGTTCGTTCTCCATGACAAAAGCAACAACCCGGAACTCCTTCCTAACCATAAhdIBaeIBplIBplIBpmIBsmIAflIIBstEIIK454KV456IE458VG460KN462DD464LE466DS468KS470LS472DD474TD476VE478VT480SE482DE484WQ486CT488EC490KK492FN494SP496SK498RY500CF502RC504WA506LR508KD510WY5121491-3533Full MDMX WT2,8602,8802,9002,9202,9402,9602,9803,0003,020
TCAGATTGTTCAAAGTTAACCCATTCTCTYTCCACGTCTGATATCACTGCCATACCTGAAAAGGAAAATGAAGGAAATGATGTCCCTGATTGTCGAAGAACCATTTCGGCTCCTGTCGTTAGACCTAAAGATGCGTATATAAAGAAAGAAAACTCCAAACTTTTTGATCCCTGCAACTAGTCTAACAAGTTTCAATTGGGTAAGAGARAGGTGCAGACTATAGTGACGGTATGGACTTTTCCTTTTACTTCCTTTACTACAGGGACTAACAGCTTCTTGGTAAAGCCGAGGACAGCAATCTGGATTTCTACGCATATATTTCTTTCTTTTGAGGTTTGAAAAACTAGGGACGTTGAHpaIBmgBISD514CS516KL518TH520SL522ST524SD526IT528AI530PE532KE534NE536GN538DV540PD542CR544RT546IS548AP550VV552RP554KD556AY558IK560KE562NS564KL566FD568PC570N1491-3533Full MDMX WT3,0403,0603,0803,1003,1203,1403,1603,1803,200
CAGTGGAATTYTTGGATTTGGCTCACAGTTCTGAAAGCCAAGAGACCATYTCAAGCATGGGAGAACAGTTAGATAACCTTTCTGAACAGAGAACAGATACAGAAAACATGGAGGATTGCCAGAATCTCTTGAAGCCATGTAGCTTATGTGAGAAAAGACCACGAGACGGGAACATTATGTCACCTTAARAACCTAAACCGAGTGTCAAGACTTTCGGTTCTCTGGTARAGTTCGTACCCTCTTGTCAATCTATTGGAAAGACTTGTCTCTTGTCTATGTCTTTTGTACCTCCTAACGGTCTTAGAGAACTTCGGTACATCGAATACACTCTTTTCTGGTGCTCTGCCCTTGTAATAS572VE574FL576DL578AH580SS582ES584QE586TI588SS590MG592EQ594LD596NL598SE600QR602TD604TE606NM608ED610CQ612NL614LK616PC618SL620CE622KR624PR626DG628NI630I1491-3533Full MDMX WT3,2203,2403,2603,2803,3003,3203,3403,3603,380
TCATGGAAGGACGGGCCATCTTGTCACTTGTTTTCACTGTGCCAGAAGACTAAAGAAGGCTGGGGCTTCATGCCCTATTTGCAAGAAAGAGATTCAGCTGGTTATTAAGGTTTTTATAGCATAAGGATCCatactgaaaaaccccgcaagttcacttcaactgtgcatcgtgcaccatAGTACCTTCCTGCCCGGTAGAACAGTGAACAAAAGTGACACGGTCTTCTGATTTCTTCCGACCCCGAAGTACGGGATAAACGTTCTTTCTCTAAGTCGACCAATAATTCCAAAAATATCGTATTCCTAGGtatgactttttggggcgttcaagtgaagttgacacgtagcacgtggtaBamHIH632GR634TG636HL638VT640CF642HC644AR646RL648KK650AG652AS654CP656IC658KK660EI662QL664VI666KV668FI670A*672GS674IL676KN678PA680S1491-3533Full MDMX WT3,4003,4203,4403,4603,4803,5003,5203,5403,560
ctcaatttctttcatttatacatcgttttgccttcttttatgtaactatactcctctaagtttcaatcttggccatgtaacctctgatctatagaattttttaaatgactagaattaatgcccatcttttttttggacctaaattcttcatgaaaatatattacgagggcttattcaggagttaaagaaagtaaatatgtagcaaaacggaagaaaatacattgatatgaggagattcaaagttagaaccggtacattggagactagatatcttaaaaaatttactgatcttaattacgggtagaaaaaaaacctggatttaagaagtacttttatataatgctcccgaataagtc3,5803,6003,6203,6403,6603,6803,7003,720
aagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagtttcgaaacctgaagaagcggtctccaaaccagttcagaggttagttccaacagccgaacagatggaacggtctttaaatgcttttctaccttttcccagtttagcaaccatctatgcaacaactgtgaagatttattcgcttaaagaatactaaatactaaaaataataatttattcaBstXIHindIIIADH1\transcription\termination\signal3,7403,7603,7803,8003,8203,8403,8603,8803,900
tataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatgaacataatattttttttattcacatatgtttaaaatttcactgagaatccaaaattttgcttttaagaataagaactcattgagaaaggacatccagtccaacgaaagagtccatatcgtactccagcgagaataactggtgtggagatggccggccagctttaaggggatgggatacttgtatADH1\transcription\termination\signal3,9203,9403,9603,9804,0004,0204,0404,0604,080
ttccattttgtaatttcgtgtcgtttctattatgaatttcatttataaagtttatgtacaaatatcataaaaaaagagaatctttttaagcaaggattttcttaacttcttcggcgacagcatcaccgacttcggtggtactgttggaaccacctaaatcaccagttctgatacctgcaaggtaaaacattaaagcacagcaaagataatacttaaagtaaatatttcaaatacatgtttatagtatttttttctcttagaaaaattcgttcctaaaagaattgaagaagccgctgtcgtagtggctgaagccaccatgacaaccttggtggatttagtggtcaagactatggacgLEU24,1004,1204,1404,1604,1804,2004,2204,2404,260
atccaaaacctttttaactgcatcttcaatggccttaccttcttcaggcaagttcaatgacaatttcaacatcattgcagcagacaagatagtggcgatagggttgaccttattctttggcaaatctggagcagaaccgtggcatggttcgtacaaaccaaatgcggtgttcttgtcttaggttttggaaaaattgacgtagaagttaccggaatggaagaagtccgttcaagttactgttaaagttgtagtaacgtcgtctgttctatcaccgctatcccaactggaataagaaaccgtttagacctcgtcttggcaccgtaccaagcatgtttggtttacgccacaagaacagaBpmIEcoNILEU24,2804,3004,3204,3404,3604,3804,4004,4204,440
ggcaaagaggccaaggacgcagatggcaacaaacccaaggaacctgggataacggaggcttcatcggagatgatatcaccaaacatgttgctggtgattataataccatttaggtgggttgggttcttaactaggatcatggcggcagaatcaatcaattgatgttgaaccttcaatgccgtttctccggttcctgcgtctaccgttgtttgggttccttggaccctattgcctccgaagtagcctctactatagtggtttgtacaacgaccactaatattatggtaaatccacccaacccaagaattgatcctagtaccgccgtcttagttagttaactacaacttggaagttacBstXILEU24,4604,4804,5004,5204,5404,5604,5804,6004,620
taggaaattcgttcttgatggtttcctccacagtttttctccataatcttgaagaggccaaaacattagctttatccaaggaccaaataggcaatggtggctcatgttgtagggccatgaaagcggccattcttgtgattctttgcacttctggaacggtgtattgttcactatcccaatcctttaagcaagaactaccaaaggaggtgtcaaaaagaggtattagaacttctccggttttgtaatcgaaataggttcctggtttatccgttaccaccgagtacaacatcccggtactttcgccggtaagaacactaagaaacgtgaagaccttgccacataacaagtgatagggtLEU24,6404,6604,6804,7004,7204,7404,7604,7804,800
agcgacaccatcaccatcgtcttcctttctcttaccaaagtaaatacctcccactaattctctgacaacaacgaagtcagtacctttagcaaattgtggcttgattggagataagtctaaaagagagtcggatgcaaagttacatggtcttaagttggcgtacaattgaagttctttatcgctgtggtagtggtagcagaaggaaagagaatggtttcatttatggagggtgattaagagactgttgttgcttcagtcatggaaatcgtttaacaccgaactaacctctattcagattttctctcagcctacgtttcaatgtaccagaattcaaccgcatgttaacttcaagaaatAflIILEU24,8204,8404,8604,8804,9004,9204,9404,9604,980
cggatttttagtaaaccttgttcaggtctaacactacctgtaccccatttaggaccacccacagcacctaacaaaacggcatcagccttcttggaggcttccagcgcctcatctggaagtgggacacctgtagcttcgatagcagcaccaccaattaaatgattttcgaaatcgaactgcctaaaaatcatttggaacaagtccagattgtgatggacatggggtaaatcctggtgggtgtcgtggattgttttgccgtagtcggaagaacctccgaaggtcgcggagtagaccttcaccctgtggacatcgaagctatcgtcgtggtggttaatttactaaaagctttagcttgaAjuIPsrIBstBIEcoNILEU25,0005,0205,0405,0605,0805,1005,1205,1405,160
tgacattggaacgaacatcagaaatagctttaagaaccttaatggcttcggctgtgatttcttgaccaacgtggtcacctggcaaaacgacgatcttcttaggggcagacattagaatggtatatccttgaaatatatatatatattgctgaaatgtaaaaggtaagaaaagttagaaactgtaaccttgcttgtagtctttatcgaaattcttggaattaccgaagccgacactaaagaactggttgcaccagtggaccgttttgctgctagaagaatccccgtctgtaatcttaccatataggaactttatatatatatataacgactttacattttccattcttttcaatcttBstEIILEU25,1805,2005,2205,2405,2605,2805,3005,3205,340
agtaagacgattgctaaccacctattggaaaaaacaataggtccttaaataatattgtcaacttcaagtattgtgatgcaagcatttagtcatgaacgcttctctattctatatgaaaagccggttccggcgctctcacctttcctttttctcccaatttttcagttgaaaaaggtattcattctgctaacgattggtggataaccttttttgttatccaggaatttattataacagttgaagttcataacactacgttcgtaaatcagtacttgcgaagagataagatatacttttcggccaaggccgcgagagtggaaaggaaaaagagggttaaaaagtcaactttttccataPflMIPpuMI5,3605,3805,4005,4205,4405,4605,4805,500
atgcgtcaggcgacctctgaaattaacaaaaaatttccagtcatcgaatttgattctgtgcgatagcgcccctgtgtgttctcgttatgttgaggaaaaaaataatggttgctaagagattcgaactcttgcatcttacgatacctgagtattcccacagttgggggatctcgactcttacgcagtccgctggagactttaattgttttttaaaggtcagtagcttaaactaagacacgctatcgcggggacacacaagagcaatacaactcctttttttattaccaacgattctctaagcttgagaacgtagaatgctatggactcataagggtgtcaaccccctagagctgagaBaeIBstBI5,5205,5405,5605,5805,6005,6205,6405,6605,680
agctagaggatcaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaatcgatctcctagttaagcattagtacagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttlac promoterLacO5,7005,7205,7405,7605,7805,8005,8205,8405,860
cctgtcgtgccagctgataacttcgtataatgtatgctatacgaagttattaggtctgaagaggagtttacgtccagccaagctagcttggctgcaggtcgagcggccgcgatccggaacccttaatataacttcgtataatgtatgctatacgaagttatcagctgcattaatgaatggacagcacggtcgactattgaagcatattacatacgatatgcttcaataatccagacttctcctcaaatgcaggtcggttcgatcgaaccgacgtccagctcgccggcgctaggccttgggaattatattgaagcatattacatacgatatgcttcaatagtcgacgtaattacttaBmtINheINotIPstIBsaXIBspEILoxPLoxPpUC...in5,8805,9005,9205,9405,9605,9806,0006,0206,040
cggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcgccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccgBsaXIpUC\origin6,0606,0806,1006,1206,1406,1606,1806,2006,220
cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgagtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctpUC\originColE1 origin6,2406,2606,2806,3006,3206,3406,3606,3806,400
ccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactpUC\originColE1 origin6,4206,4406,4606,4806,5006,5206,5406,5606,580
gtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaacaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagccttttpUC\originColE1 origin6,6006,6206,6406,6606,6806,7006,7206,7406,760
agagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaColE1 originpUC\origin6,7806,8006,8206,8406,8606,8806,9006,9206,940
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagtcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcagtcttcttgagcagttcttccgctatcttccgctacgcgacgcttagccctcgccgctatggcatttcgtgctccttcgccagtcgNeoR/KanR6,9606,9807,0007,0207,0407,0607,0807,1007,120
ccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgagtcacgacgagatcctcgccgtcgggcatgggtaagcggcggttcgagaagtcgttatagtgcccatcggttgcgatacaggactatcgccaggcggtgtgggtcggccggtgtcagctacttaggtcttttcgccggtaaaaggtggtactataagccgttcgtccgtagcggtactcagtgctgctctaggagcggcagcccgtacNaeIRsrIINgoMIVNeoR/KanR7,1407,1607,1807,2007,2207,2407,2607,280
ctcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatgagcggaactcggaccgcttgtcaagccgaccgcgctcggggactacgagaagcaggtctagtaggactagctgttctggccgaaggtaggctcatgcacgagcgagctacgctacaaagcgaaccaccagcttacccgtccatcggcctagttcgcatacgtcggcggcgtaacgtaBanIIBsaAINeoR/KanR7,3007,3207,3407,3607,3807,4007,4207,4407,460
cagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcttgcagttcgtcggtactacctatgaaagagccgtcctcgttccactctactgtcctctaggacggggccgtgaagcgggttatcgtcggtcagggaagggcgaagtcactgttgcagctcgtgtcgacgcgttccttgcgggcagcaccggtcggtgctatcggcgcgacggagcagaacgtcaagPflFINmeAIIITth111INeoR/KanR7,4807,5007,5207,5407,5607,5807,6007,6207,640
attcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatcatactctaagtcccgtggcctgtccagccagaactgtttttcttggcccgcggggacgcgactgtcggccttgtgccgccgtagtctcgtcggctaacagacaacacgggtcagtatcggcttatcggagaggtgggttcgccggcctcttggacgcacgttaggtagaacaagttagtatgagKasINarISfoIPluTINeoR/KanR7,6607,6807,7007,7207,7407,7607,7807,8007,820
ttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtaaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatZraIAatIIAMP promoter7,8407,8607,8807,9007,9207,9407,9607,9808,000
tcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagaagtgctccgggaaagcagagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgaccgaattgatacgccgtagtct8,0208,0408,0608,0808,1008,1208,1408,1608,180
gcagattgtactgagagtgcaccataacgcatttaagcataaacacgcactatgccgttcttctcatgtatatatatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatgtgcgcagctcgcgttgcattttcggaagcgctcgttttcggaaacgctttgacgtctaacatgactctcacgtggtattgcgtaaattcgtatttgtgcgtgatacggcaagaagagtacatatatatatatgtccgttgtgcgtctatatccacgctgcacttgtcactcgacatacacgcgtcgagcgcaacgtaaaagccttcgcgagcaaaagcctttgcgaaactBmgBI2 micron origin8,2008,2208,2408,2608,2808,3008,3208,3408,360
agttcctattccgaagttcctattctctagctagaaagtataggaacttcagagcgcttttgaaaaccaaaagcgctctgaagacgcactttcaaaaaaccaaaaacgcaccggactgtaacgagctactaaaatattgcgaataccgcttccacaaacattgctcaaaagtatctcttcaaggataaggcttcaaggataagagatcgatctttcatatccttgaagtctcgcgaaaacttttggttttcgcgagacttctgcgtgaaagttttttggtttttgcgtggcctgacattgctcgatgattttataacgcttatggcgaaggtgtttgtaacgagttttcatagagaCspCI2 micron originYeast\2\micron\origin8,3808,4008,4208,4408,4608,4808,5008,5208,540
ttgctatatatctctgtgctatatccctatataacctacccatccacctttcgctccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctttagacaaaaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaacgatatatagagacacgatatagggatatattggatgggtaggtggaaagcgaggaacttgaacgtagatttgagctggagatgtaaaaaatacaaatagagatcataatgagaaatctgtttttttaacatcattcttgataagtatctcacttagcttttgttatgcttttaca2 micron originYeast\2\micron\origin8,5608,5808,6008,6208,6408,6608,6808,7008,720
aaacatttcctatacgtagtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatcactttctgttcacaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaaaaaatgcacccgcagcttcgcttttgtaaaggatatgcatcatatatctctgttttatcttctttggcaagtattaaaagactggttacttcttagtagttgcgatagtgaaagacaagtgtttcatacgcgttaggtgtagccatatcttatattagcccctacggaaatagaacttttttacgtgggcgtcgaagcgaBsaAISnaBI2 micron originYeast\2\micron\origin8,7408,7608,7808,8008,8208,8408,8608,8808,900
agtaatcagtaaacgcgggaagtggagtcaggctttttttatggaagagaaaatagacaccaaagtagccttcttctaaccttaacggacctacagtgcaaaaagttatcaagagactgcattatagagcgcacaaaggagaaaaaaagtaatctaagatgctttgttagaaaaatagtcattagtcatttgcgcccttcacctcagtccgaaaaaaataccttctcttttatctgtggtttcatcggaagaagattggaattgcctggatgtcacgtttttcaatagttctctgacgtaatatctcgcgtgtttcctctttttttcattagattctacgaaacaatctttttatcAjuI2 micron originYeast\2\micron\origin8,9208,9408,9608,9809,0009,0209,0409,060
cgctctcgggatgcatttttgtagaacaaaaaagaagtatagattctttgttggtaaaatagcgctctcgcgttgcatttctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttttacaaaaatgaagcacagattcttcgttggcgagagccctacgtaaaaacatcttgttttttcttcatatctaagaaacaaccattttatcgcgagagcgcaacgtaaagacaagacatttttacgtcgagtctaagaaacaaactttttaatcgcgagagcgcaacgtaaaaacaaaatgtttttacttcgtgtctaagaagcaacAvaINsiIBsoBI2 micron originYeast\2\micron\origin9,0809,1009,1209,1409,1609,1809,2009,2209,240
gtaaaatagcgctttcgcgttgcatttctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcacagatgcttcgttgctcattttatcgcgaaagcgcaacgtaaagacaagacatttttacgtcgagtctaagaaacaaactttttaatcgcgagagcgcaacgtaaaaacaagatgttttacttcgtgtctacgaagcaacga2 micron originYeast\2\micron\origin9,2609,2809,3009,3209,3409,3609,380
  • Plasmid
Editing disabled on read-only sequence.
9382 bp700075008000850090005001000150020001491-3533MDMX FL Ins Rev pGAD (DNA)pGAD Vector MKII F (DNA)pGAD3.0 F (DNA)pGBK3.0 F (DNA)AB Seq Primer 1 (DNA)PstI,SbfI,KasI,NarI,SfoI,PluTI,NgoMIV,NaeI,BglI,Bpu10I,BsmIScaIADH1\Promoter,ADH1 promoter,PacIBsaBIBsgIHindIIIGAL4\DNA\binding\domain,Gal4 DNA-binding domain,AvaI,BsoBI,+3HpaIT7,T7\RNA\Polymerase\Promoterc-Myc\tag,MCS,pGAD3 Vector R (DNA),+7PaqCI,BstAPIBclINdeI,BglII,ZraI,AatIIRsrII,NgoMIV,NaeIBanII,BsaAINeoR/KanR,NmeAIIIPflFI,Tth111IKasI,NarI,SfoI,PluTIAMP promoter,Kan PGAD R (DNA)ZraIAatIIBmgBICspCI2 micron origin,Yeast\2\micron\origin,BsaAI,SnaBIAjuI,AvaI,BsoBI,NsiI

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9382