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pDS5.43: pUC19 + GFP
  • Sequence Map
Editing disabled on read-only sequence.
gagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtctctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcaDrdIMmeIBciVIHaeIIori20406080100120140160
cgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggNspIPciITfiIBsrBIAflIIIori180200220240260280300320340
gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattatttatagctagctcagccccgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaataaatatcgatcgagtcgggAseIBlpIBmtINheISapIStyITfiIBsaXIBspQIHaeIIPvuIIAnneal SiteAnnealJ23115...moter360380400420440460480500520
ttggtacaatgctagctgagacggaaagtgaaacgtgatttcatgcgtcattttgaacattttgtaaatcttatttaataatgtgtgcggcaattcacatttaatttatgaatgttttcttaacatcgcggcaactcaagaaacggcaggttcggatcttagctactagagaaagaaccatgttacgatcgactctgcctttcactttgcactaaagtacgcagtaaaacttgtaaaacatttagaataaattattacacacgccgttaagtgtaaattaaatacttacaaaagaattgtagcgccgttgagttctttgccgtccaagcctagaatcgatgatctctttcBmtINheIXmnIBfuAIBspMIBtgZIJ2311...oter540560580600620640660680700
aggagaaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgcctcctctttatgatctacgcatttccgcttctcgacaagtgaccacagcagggataagaccaccttgacctaccactacagttgccagtattcaaaaggcacgcaccgctcccacttccactgcgttgattaccatttgactgcgacttcaagtagacatgatgaccatttgacggSapIBseRIBsmFIBspQIBsrFIHincIIMR2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VR30GE32GE34GD36AT38NG40KL42TL44KF46IC48TT50GK52LP54715-1428superfolder GFP720740760780800820840860
ggttccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgccaaggaaccggctgagaccattgctgcgactgaataccacaagtcacgaaacgagcaataggcctggtatacttcgtcgtactgaagaagttcaggcggtacggccttccgatacacgtccttgcgtgctaaaggaaattcctactgccgtgcatgttttgcgcacgccttcacBsgIMluINdeIStyIBsaAIBspEIAflIIIVP56WP58TL60VT62TL64TY66GV68QC70FA72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98SF100KD102DG104TY106KT108RA110EV112715-1428superfolder GFP8809009209409609801,0001,0201,040
aaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaggacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtttaaacttccgctatgggaccatttggcgtaactcgactttccgtaactgaaatttctcctgccgttataggacccggtattcgaccttatgttaaaattgtcggtgttacaaatgtagtggcggctatttgtttttttaccgtaatttcgcttaaaattttaagcggtgttgcBaeIPflMIPflMIDraIIIKF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144FN146SH148NV150YI152TA154DK156QK158NG160IK162AN164FK166IR168HN170715-1428superfolder GFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
tggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatacctcctaccgtcgcacgtcgaccgactagtgatggtcgttttgtgaggttagccactaccaggacaagacgacggtctgttagtgatagactcgtgcgtttcgcaagacagatttctaggcttgctctttgcgctagtataccaagacgacctcaagcattggcgtcgcccgtaBclIBpmIBsgINdeIPvuIIVE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SV206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228I715-1428superfolder GFP1,2401,2601,2801,3001,3201,3401,3601,3801,400
cacgcatggtatggatgaactgtacaaatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgctgatgccgcatagttaagccgtgcgtaccatacctacttgacatgtttactggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcgactacggcgtatcaattcggBmrIPsrIBsrGIT230HG232MD234EL236YK238715-1428rrnB T1 terminatorsuperfolder GFPT7Te terminatorAnnealAnneallacZα1,4201,4401,4601,4801,5001,5201,5401,560
agccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaDrdINspIPfoIEcoO109I1,5801,6001,6201,6401,6601,6801,7001,7201,740
taatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttSspIZraIAatIIBciVIBsaHIBsrBIAmpR promoterAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
catttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgAclIApaLIBaeGIAmpRAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
gttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacBcgIScaIXmnIBsaHIAmpR2,1202,1402,1602,1802,2002,2202,2402,260
cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacggtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgcPvuIAmpR2,2802,3002,3202,3402,3602,3802,4002,4202,440
atgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgAclIAseIBglIFspIBsrDIBsrFINmeAIIIAmpR2,4602,4802,5002,5202,5402,5602,5802,6002,620
gtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatataAhdIBanIBmrIBpmIBsaIBsrDIAmpR2,6402,6602,6802,7002,7202,7402,7602,7802,800
actttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgori2,8202,8402,8602,8802,9002,9202,9402,960
tgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtaori2,9803,0003,0203,0403,0603,0803,1003,1203,140
acctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaacttggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgaMmeIAlwNIApaLIBaeGIBseYIori3,1603,1803,2003,2203,2403,2603,2803,300
  • Plasmid
Editing disabled on read-only sequence.
3316 bp2250250027503000250500750AmpRAnnealori715-1428ArbGibGFP_60bp_F (DNA)ArbGibGFP_40bp_F (DNA)ArbGibBB_60bp_R (DNA)ArbGibBB_40bp_R (DNA)ArbGibBB_40bp_F (DNA)ArbGibGFP_20bp_R (DNA)ArbGibGFP_40bp_R (DNA)ArbGibGFP_60bp_R (DNA)ArbGibBB_20bp_F (DNA)AmpRemoveR (DNA)

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3316