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pDS5.29: pBR322 backbone + tfoX cassette + GFP
  • Sequence Map
    Editing disabled on read-only sequence.
    atgcataatgtgcctgtcaaatggacgaagcagggattctgcaaaccctatgctactccctcgagccgtcaattgtctgattcgttaccaattaggatccttatcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagatacgtattacacggacagtttacctgcttcgtccctaagacgtttgggatacgatgagggagctcggcagttaacagactaagcaatggttaatcctaggaatagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctAvaIMfeITliIXhoIBamHIBsaBIBsoBIPspXIBstAPIPaeR7I*Q360GS358EL356RS354VQ352RA350LQ348ML346SD344AL342AR340PS338104-1186LacIIOT20406080100120140160
    ggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagactggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgaccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcHpaIBsaXIAT336QT334NP332AL330TT328KR326KV324LS322VP320LL318QN316GK314VA312QG310QS308LQ306LL304RD302VS300TQ298GL296LR294FD292QK290IT288TL286PP284IY282CS280104-1186LacI180200220240260280300320340
    ctatcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcacgatagaagccatagcagcatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgBciVIEcoRVBssHIISD278ET276DD274YG272VV270SI268DA266GV264RL262GS260ET258IA256RM254AG252LA250MQ248DN246AV244LM242AT240PV238IG236EN234LM232QM230TQ228QF226GS224MA222S104-1186LacI360380400420440460480500520
    tccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcctcatgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcaggagtacApaIBcgIBstEIIPspOMINmeAIIIW220DG218ER216EA214IP212QI210QN208RT206LY204KH202WG200AL198RL196RA194SV192SS190LP188GA186LL184AI182QQ180HG178LA176VL174HE172VG170LR168TG166DE164H104-1186LacI540560580600620640660680700
    ggagtaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgcctcatttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagcBclIMluIS162YI160IS158NI156PT154QD152SV150DL148FL146AP144VN142TC140AA138EV136AI134AD132QD130DL128PY126NI124IL122GS120VR118QA116LL114NH112VA110AK108CA106E104-1186LacI720740760780800820840860
    acgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaV104GS102RE100VM98SV96VV94SA92GL90QD88AR86SK84IA82AV80IQ78SP76AH74LA72LS70ST68AV66GI64LL62SQ60KG58AL56QQ54AV52RN50PI48YN46104-1186LacI8809009209409609801,0001,0201,040
    tcagctccaccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtcatataagagacaccggcatactctgcgacatcgtataacgttactggtttcatattcaccaccctgaattgactctcttccgggcgctatcaagtcgaggtggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagtatattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtataagtggtgggacttaactgagagaaggcccgcgatagtAclIBstAPILE44VM42AA40EV38KE36RT34KA32SV30HS28AQ26NV24VR22SV20TM18YS16VG14AY12EA10VD8YL6TV4PK2M104-1186LacIPLacI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccataccgcgaaaggttttgcgccattcggctgtgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcgctcacaatttactagagaaagaggagaaataatcaatggatatgaatgagcaacagtttttcgactacgtaactaagtttggcgcctaccaaaacggtatggcgctttccaaaacgcggtaagccgacacaactgttaattagtagccgagcatattacacaccttaacactcgcgagtgttaaatgatctctttctcctctttattagttacctatacttactcgttgtcaaaaagctgatgcattgattcaaaccgcggatggtttAfeIBseRISnaBIMD2MN4EQ6QF8FD10YV12TK14FG16AY18Q1343-1933Tfox V. choleraePtacPLacIR_B00341,2401,2601,2801,3001,3201,3401,3601,3801,400
    aacgctcgatgtttggtggtattggcttgtttcaacacgacgctatgtatgtgttggtcagtgaagaccgcatttttgtgcgtggtggagaagagctcgaccctgagctcttggccttagggtgcgagaaatatcgccatgttaaaaaacagacaacggcaaccgtaaactattattgcgagctacaaaccaccataaccgaacaaagttgtgctgcgatacatacacaaccagtcacttctggcgtaaaaacacgcaccacctcttctcgagctgggactcgagaaccggaatcccacgctctttatagcggtacaattttttgtctgttgccgttggcatttgataatBbsISacISacIBsu36IEco53kIEco53kIK20RS22MF24GG26IG28LF30QH32DA34MY36VL38VS40ED42RI44FV46RG48GE50EL52DP54EL56LA58LG60CE62KY64RH66VK68KQ70TT72AT74VN76YY781343-1933Tfox V. cholerae1,4201,4401,4601,4801,5001,5201,5401,560
    cgacatcactgaactgtatgagcaacatcaccctgagctagactcgattattgaacgctcgattcagttttctgtgaatcagcgggaatttcaacgctcagcagccagtcgtcgtctacgggatttacccaatatgcaactgactttggagcgtatggtaaaaaaagcgggcattgctgtagtgacttgacatactcgttgtagtgggactcgatctgagctaataacttgcgagctaagtcaaaagacacttagtcgcccttaaagttgcgagtcgtcggtcagcagcagatgccctaaatgggttatacgttgactgaaacctcgcataccatttttttcgcccgtaaAccIDI80TE82LY84EQ86HH88PE90LD92SI94IE96RS98IQ100FS102VN104QR106EF108QR110SA112AS114RR116LR118DL120PN122MQ124LT126LE128RM130VK132KA134GI1361343-1933Tfox V. cholerae1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gacgatgtggaaacttttatgagcctcggtgcgccagaagtgtttaataaggtgcgtcaagcttatggaagtgatgtcgatgtaaaactactgtggaagtttgcgggtgcgattgaaggcattcactggaaactgctgcaagagccgcgtaagcgccaactgttagaaagttgtcctgctacacctttgaaaatactcggagccacgcggtcttcacaaattattccacgcagttcgaataccttcactacagctacattttgatgacaccttcaaacgcccacgctaacttccgtaagtgacctttgacgacgttctcggcgcattcgcggttgacaatctttcaacagHindIIIDD138VE140TF142MS144LG146AP148EV150FN152KV154RQ156AY158GS160DV162DV164KL166LW168KF170AG172AI174EG176IH178WK180LL182QE184PR186KR188QL190LE192SC1941343-1933Tfox V. cholerae1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agcagcgtgcttaaaaaaaaaaaccccgcccctgacagggcggggttttttttcgcttactagcgtataaacgcaggctgagcagttacagagatgttacgaaccactagtgcactgcagtacagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaatcgtcgcacgaatttttttttttggggcggggactgtcccgccccaaaaaaaagcgaatgatcgcatatttgcgtccgactcgtcaatgtctctacaatgcttggtgatcacgtgacgtcatgtcacaatgttggttaattggttaagactaatctttttgagtagctcgtagttPstISpeIQQ196R*272FF270ED268LM266L2077-2892B1006KanRAnnealAnnealB00151,9401,9601,9802,0002,0202,0402,0602,0802,100
    atgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcatactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228QY226RD224AI222GV220RG218VD216IC214GI212LK210GE208D2077-2892KanR2,1202,1402,1602,1802,2002,2202,2402,260
    aaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgaggcttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcgaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctccgAjuIEcoNIHindIIIF206IL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED168DF166DS164AD162VL160GN158NM156RS154QA152QA150LR1482077-2892KanR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcaAvaIPvuISmaISspIXmaIAsiSIBsoBIEcoNITspMIFV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP102IA100TT98LL96WA94DD92PT902077-2892KanR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    acggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatClaIBspDIRI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY44LR42YI40TA38GS36QG34VN322077-2892KanR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggacctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactactatataaaaatagaacacgttacattgtagNruIDR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2M2077-2892KanRPromoter??pENTR-RKan-R2,8202,8402,8602,8802,9002,9202,9402,960
    agagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcaggagttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgtctctaaaactctgtgttgcaccgaaacaacttatttagcttgaaaacgactcaacttcctagtcctcaagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacAloIDraIIIAnnealori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacagacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtAcuIori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagataccatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggAlwNIori3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaaDrdIBciVIBssSIBssSαIoripBR322ori-F3,5203,5403,5603,5803,6003,6203,6403,660
    gtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggctNspIPciIBsrBIori3,6803,7003,7203,7403,7603,7803,8003,8203,840
    acgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcaatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtctgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgttaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagAccIPflFIBstZ17ITth111IbompRS-markerL44403,8603,8803,9003,9203,9403,9603,9804,0004,020
    atggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccttctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggttaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggaagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccaBcgIDrdINspIPfoI*64L63N62E61G60D59D58G57F56R55A54L53C52S51R50Y49L48E47D46A45H44D434136-4327roppGEX 3'bom4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatccctgaattcgcatcgcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtagggacttaagcgtagXbaIEcoRIH42L41S40E39C38I37D36A35Q34E33D32A31D30L29E28N27L26K25E24L23L22T21L20T19Q18S17R16I15F14R13A12M11N10L9A8T7K6E5Q4K3T2V14136-4327rop4,2204,2404,2604,2804,3004,3204,3404,360
    tagactgaactggccgataattgcagacgaacggatttactttacagctagctcagtcctaggtattatgctagcgctagctttatagctagctcagcccttggtacaatgctagctgagacggaaagtgaaacgtgatttcatgcgtcattttgaacattttgtaaatcttattatctgacttgaccggctattaacgtctgcttgcctaaatgaaatgtcgatcgagtcaggatccataatacgatcgcgatcgaaatatcgatcgagtcgggaaccatgttacgatcgactctgcctttcactttgcactaaagtacgcagtaaaacttgtaaaacatttagaataaAfeIAvrIIBsmBIEsp3IJ23115 PromoterAnnealAnneal4,3804,4004,4204,4404,4604,4804,5004,5204,540
    taataatgtgtgcggcaattcacatttaatttatgaatgttttcttaacatcgcggcaactcaagaaacggcaggttcggatcttagctactagagaaagaggagaaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgattattacacacgccgttaagtgtaaattaaatacttacaaaagaattgtagcgccgttgagttctttgccgtccaagcctagaatcgatgatctctttctcctctttatgatctacgcatttccgcttctcgacaagtgaccacagcagggataagaccaccttgacctaccacBfuAIBseRIBsmFIBspMIMR2KG4EE6LF8TG10VV12PI14LV16EL18DG204665-5378superfolder GFPstr...0)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggttccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaatacagttgccagtattcaaaaggcacgcaccgctcccacttccactgcgttgattaccatttgactgcgacttcaagtagacatgatgaccatttgacggccaaggaaccggctgagaccattgctgcgactgaataccacaagtcacgaaacgagcaataggcctggtatacttAcuINdeIBspEIDV22NG24HK26FS28VR30GE32GE34GD36AT38NG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FA72RY74PD76HM78K4665-5378superfolder GFP4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaggacggcaatatcctgcgtcgtactgaagaagttcaggcggtacggccttccgatacacgtccttgcgtgctaaaggaaattcctactgccgtgcatgttttgcgcacgccttcactttaaacttccgctatgggaccatttggcgtaactcgactttccgtaactgaaatttctcctgccgttataggacBaeIDraIMluIQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98SF100KD102DG104TY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136L4665-5378superfolder GFP4,9204,9404,9604,9805,0005,0205,0405,060
    ggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgcccggtattcgaccttatgttaaaattgtcggtgttacaaatgtagtggcggctatttgtttttttaccgtaatttcgcttaaaattttaagcggtgttgcacctcctaccgtcgcacgtcgaccgactagtgatggtcgttttgtgaggttagccactaccaggacaagacgacgBclIDraIDraIIIG138HK140LE142YN144FN146SH148NV150YI152TA154DK156QK158NG160IK162AN164FK166IR168HN170VE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194L4665-5378superfolder GFP5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccgtctgttagtgatagactcgtgcgtttcgcaagacagatttctaggcttgctctttgcgctagtataccaagacgacctcaagcattggcgtcgcccgtagtgcgtaccatacctacttgacatgtttactggtccgtagtttattttgctttccgagtcagctttctgacccggNdeIBsrGIP196DN198HY200LS202TQ204SV206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230HG232MD234EL236YK2384665-5378superfolder GFPrrnB T1 terminator5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgtctcacagataaaatatttgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaacaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcaaatatgcagagtgtctattttataaacgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttgPsrISspIBsmBIEsp3IrrnB T1 terminatorT7Te terminatorAnnealAnnealIOT5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatctgctcatgtttgacagcttatcatcggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagacgagtacaaactgtcgaatagtagcClaINaeIBspDISgrAINgoMIVIOT5,6205,6405,660
    • Plasmid
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    5678 bp42504500475050005250550025050075010001250CDS OverhangLacI4665-53781343-1933104-11864136-4327528_BB_F (DNA)GFP_Liftout_R (DNA)

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    5678