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pKW387.pCI5-CasRx-NES.lyp-HA
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    TGACATTGATTATTGactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctcgtgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatACTGTAACTAATAACtgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggagcactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttaSpeIregulatorysource:Human betaherpesvirus 5pCN-...ard20406080100120140160
    agggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccggccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcaggccgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaatgccctgaaaggatgaaccgtcatNdeIregulatorysource:Human betaherpesvirus 5180200220240260280300320340
    catctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtggttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattattggggCspCISnaBIregulatorysource:Human betaherpesvirus 5360380400420440460480500520
    gccccgttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagTcgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggccgggaacggtgcattggaacgcggacggggcaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagcaaatcacttggcagtctagcggacctcAgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcgccggcccttgccacgtaaccttgcgcctAjuIAloIEagIsource:Human betaherpesvirus 5exonmRNAreg...ry540560580600620640660680700
    ttccccgtgccaagagtgacgtaagtaccgcctatagactctataggcacacccctttggctcttatgcatgctatactgtttttggcttggggcctatacacccccgctccttatgctataggtgatggtatagcttagcctataggtgtgggttattgaccattattgaccacaaggggcacggttctcactgcattcatggcggatatctgagatatccgtgtggggaaaccgagaatacgtacgatatgacaaaaaccgaaccccggatatgtgggggcgaggaatacgatatccactaccatatcgaatcggatatccacacccaataactggtaataactggtgBaeIBlpINsiISphIsource:Human betaherpesvirus 5intronexonmRNA720740760780800820840860
    tcccctattggtgacgatactttccattactaatccataacatggctctttgccacaactatctctattggctatatgccaatactctgtccttcagagactgacacggactctgtatttttacaggatggggtcccatttattatttacaaattcacatatacaacaacgccgtaggggataaccactgctatgaaaggtaatgattaggtattgtaccgagaaacggtgttgatagagataaccgatatacggttatgagacaggaagtctctgactgtgcctgagacataaaaatgtcctaccccagggtaaataataaatgtttaagtgtatatgttgttgcggcaKflIXcmIsource:Human betaherpesvirus 5intron8809009209409609801,0001,0201,040
    cccccgtgcccgcagtttttattaaacatagcgtgggatctccacgcgaatctcgggtacgtgttccggacatgggctcttctccggtagcggcggagcttccacatccgagccctggtcccatgcctccagcggctcatggtcgctcggcagctccttgctcctaacagtggaggggggcacgggcgtcaaaaataatttgtatcgcaccctagaggtgcgcttagagcccatgcacaaggcctgtacccgagaagaggccatcgccgcctcgaaggtgtaggctcgggaccagggtacggaggtcgccgagtaccagcgagccgtcgaggaacgaggattgtcacctcSapIBspQIAflIIIsource:Human betaherpesvirus 5intron1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gccagacttaggcacagcacaatgcccaccaccaccagtgtgccgcacaaggccgtggcggtagggtatgtgtctgaaaatgagctcggagattgggctcgcaccgtgacgcagatggaagacttaaggcagcggcagaagaagatgcaggcagctgagttgttgtattctgatacggtctgaatccgtgtcgtgttacgggtggtggtggtcacacggcgtgttccggcaccgccatcccatacacagacttttactcgagcctctaacccgagcgtggcactgcgtctaccttctgaattccgtcgccgtcttcttctacgtccgtcgactcaacaacataagactatAleIAflIIPvuIIsource:Human betaherpesvirus 5intron1,2401,2601,2801,3001,3201,3401,3601,3801,400
    agagtcagaggtaactcccgttgcggtgctgttaacggtggagggcagtgtagtctgagcagtactcgttgctgccgcgcgcgccaccagacataatagctgacagactaacagactgttcctttccatgggtcttttctgcagtcaccgtccttgacacggctagcggtaccgctctcagtctccattgagggcaacgccacgacaattgccacctcccgtcacatcagactcgtcatgagcaacgacggcgcgcgcggtggtctgtattatcgactgtctgattgtctgacaaggaaaggtacccagaaaagacgtcagtggcaggaactgtgccgatcgccatggcgBmtIBplIBplIHpaIKpnINheIPstIMauBIAcc65IBssHIIBssHIIsource:Human betaherpesvirus 5intronexonmRNApCN-...rsemul...es1,4201,4401,4601,4801,5001,5201,5401,560
    caccatgagcGAGGCCAGCATCGaaaaAaaaaagtccttcgccaagggcatgggcgtgaagtccacactcgtgtccggctccaaagtgtacatgacaaccttcgccgaaggcagcgacgccaggctggaaaagatcgtggagggcgacagcatcaggagcgtgaatgagggcgaggtggtactcgCTCCGGTCGTAGCttttTtttttcaggaagcggttcccgtacccgcacttcaggtgtgagcacaggccgaggtttcacatgtactgttggaagcggcttccgtcgctgcggtccgaccttttctagcacctcccgctgtcgtagtcctcgcacttactcccgctcAleIBsrGIBstXIRxCas13d1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gccttcagcgctgaaatggccgataaaaacgccggctataagatcggcaacgccaaattcagccatcctaagggctacgccgtggtggctaacaaccctctgtatacaggacccgtccagcaggatatgctcggcctgaaggaaactctggaaaagaggtacttcggcgagagcgcggaagtcgcgactttaccggctatttttgcggccgatattctagccgttgcggtttaagtcggtaggattcccgatgcggcaccaccgattgttgggagacatatgtcctgggcaggtcgtcctatacgagccggacttcctttgagaccttttctccatgaagccgctctcgcAfeIAfeIStuIBstZ17IRxCas13d1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctgatggcaatgacaatatttgtatccaggtgatccataacatcctggacattgaaaaaatcctcgccgaatacattaccaacgccgcctacgccgtcaacaatatctccggcctggataaggacattattggattcggcaagttctccacagtgtatacctacgacgaattcaagactaccgttactgttataaacataggtccactaggtattgtaggacctgtaacttttttaggagcggcttatgtaatggttgcggcggatgcggcagttgttatagaggccggacctattcctgtaataacctaagccgttcaagaggtgtcacatatggatgctgcttaagttEcoNIEcoRIBstZ17IRxCas13d1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agaccccgagcaccatagggccgctttcaacaataacgataagctcatcaacgccatcaaggcccagtatgacgagttcgacaacttcctcgataaccccagactcggctatttcggccaggcctttttcagcaaggagggcagaaattacatcatcaattacggcaacgaatgctctggggctcgtggtatcccggcgaaagttgttattgctattcgagtagttgcggtagttccgggtcatactgctcaagctgttgaaggagctattggggtctgagccgataaagccggtccggaaaaagtcgttcctcccgtctttaatgtagtagttaatgccgttgcttacgPsrIStuIRxCas13d2,1202,1402,1602,1802,2002,2202,2402,260
    tatgacattctggccctcctgagcggactgaggcactgggtggtccataacaacgaagaagagtccaggatctccaggacctggctctacaacctcgataagaacctcgacaacgaatacatctccaccctcaactacctctacgacaggatcaccaatgagctgaccaactcctatactgtaagaccgggaggactcgcctgactccgtgacccaccaggtattgttgcttcttctcaggtcctagaggtcctggaccgagatgttggagctattcttggagctgttgcttatgtagaggtgggagttgatggagatgctgtcctagtggttactcgactggttgaggaRxCas13d2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tctccaagaactccgccgccaacgtgaactatattgccgaaactctgggaatcaaccctgccgaattcgccgaacaatatttcagattcagcattatgaaagagcagaaaaacctcggattcaatatcaccaagctcagggaagtgatgctggacaggaaggatatgtccgagatagaggttcttgaggcggcggttgcacttgatataacggctttgagacccttagttgggacggcttaagcggcttgttataaagtctaagtcgtaatactttctcgtctttttggagcctaagttatagtggttcgagtcccttcactacgacctgtccttcctatacaggctctaEcoRIRxCas13d2,4602,4802,5002,5202,5402,5602,5802,6002,620
    caggaaaaatcataaggtgttcgactccatcaggaccaaggtctacaccatgatggactttgtgatttataggtattacatcgaagaggatgccaaggtggctgccgccaataagtccctccccgataatgagaagtccctgagcgagaaggatatctttgtgattaacctgagggtcctttttagtattccacaagctgaggtagtcctggttccagatgtggtactacctgaaacactaaatatccataatgtagcttctcctacggttccaccgacggcggttattcagggaggggctattactcttcagggactcgctcttcctatagaaacactaattggactccEcoRVPshAIRxCas13d2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ggctccttcaacgacgaccagaaggatgccctctactacgatgaagctaatagaatttggagaaagctcgaaaatatcatgcacaacatcaaggaatttaggggaaacaagacaagagagtataagaagaaggacgcccctagactgcccagaatcctgcccgctggccgtgatgccgaggaagttgctgctggtcttcctacgggagatgatgctacttcgattatcttaaacctctttcgagcttttatagtacgtgttgtagttccttaaatcccctttgttctgttctctcatattcttcttcctgcggggatctgacgggtcttaggacgggcgaccggcactacRxCas13d2,8202,8402,8602,8802,9002,9202,9402,960
    tttccgccttcagcaaactcatgtatgccctgaccatgttcctggatggcaaggagatcaacgacctcctgaccaccctgattaataaattcgataacatccagagcttcctgaaggtgatgcctctcatcggagtcaacgctaagttcgtggaggaatacgcctttttcaaagaaaaggcggaagtcgtttgagtacatacgggactggtacaaggacctaccgttcctctagttgctggaggactggtgggactaattatttaagctattgtaggtctcgaaggacttccactacggagagtagcctcagttgcgattcaagcacctccttatgcggaaaaagtttctBstXIRxCas13d2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctccgccaagatcgccgatgagctgaggctgatcaagtccttcgctagaatgggagaacctattgccgatgccaggagggccatgtatatcgacgccatccgtattttaggaaccaacctgtcctatgatgagctcaaggccctcgccgacaccttttccctggacgagaacggagaggcggttctagcggctactcgactccgactagttcaggaagcgatcttaccctcttggataacggctacggtcctcccggtacatatagctgcggtaggcataaaatccttggttggacaggatactactcgagttccgggagcggctgtggaaaagggacctgctcttgcctBbvCIRxCas13d3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aacaagctcaagaaaggcaagcacggcatgagaaatttcattattaataacgtgatcagcaataaaaggttccactacctgatcagatacggtgatcctgcccacctccatgagatcgccaaaaacgaggccgtggtgaagttcgtgctcggcaggatcgctgacatccagaaaattgttcgagttctttccgttcgtgccgtactctttaaagtaataattattgcactagtcgttattttccaaggtgatggactagtctatgccactaggacgggtggaggtactctagcggtttttgctccggcaccacttcaagcacgagccgtcctagcgactgtaggtcttttRxCas13d3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aacagggccagaacggcaagaaccagatcgacaggtactacgaaacttgtatcggaaaggataagggcaagagcgtgagcgaaaaggtggacgctctcacaaagatcatcaccggaatgaactacgaccaattcgacaagaaaaggagcgtcattgaggacaccggcagggaaaattgtcccggtcttgccgttcttggtctagctgtccatgatgctttgaacatagcctttcctattcccgttctcgcactcgcttttccacctgcgagagtgtttctagtagtggccttacttgatgctggttaagctgttcttttcctcgcagtaactcctgtggccgtcccttttRxCas13d3,5203,5403,5603,5803,6003,6203,6403,660
    cgccgagagggagaagtttaaaaagatcatcagcctgtacctcaccgtgatctaccacatcctcaagaatattgtcaatatcaacgccaggtacgtcatcggattccattgcgtcgagcgtgatgctcaactgtacaaggagaaaggctacgacatcaatctcaagaaactggaagcggctctccctcttcaaatttttctagtagtcggacatggagtggcactagatggtgtaggagttcttataacagttatagttgcggtccatgcagtagcctaaggtaacgcagctcgcactacgagttgacatgttcctctttccgatgctgtagttagagttctttgaccttBsrGIRxCas13d3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gagaagggattcagctccgtcaccaagctctgcgctggcattgatgaaactgcccccgataagagaaaggacgtggaaaaggagatggctgaaagagccaaggagagcattgacagcctcgagagcgccaaccccaagctgtatgccaattacatcaaatacagcgacgagaagactcttccctaagtcgaggcagtggttcgagacgcgaccgtaactactttgacgggggctattctctttcctgcaccttttcctctaccgactttctcggttcctctcgtaactgtcggagctctcgcggttggggttcgacatacggttaatgtagtttatgtcgctgctcttctTliIXhoIXmnIBmgBIPaeR7IRxCas13dCas13d coreRxCas13d3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aagccgaggagttcaccaggcagattaacagggagaaggccaaaaccgccctgaacgcctacctgaggaacaccaagtggaatgtgatcatcagggaggacctcctgagaattgacaacaagacatgtaccctgttcagaaacaaggccgtccacctggaagtggccaggtatgtttcggctcctcaagtggtccgtctaattgtccctcttccggttttggcgggacttgcggatggactccttgtggttcaccttacactagtagtccctcctggaggactcttaactgttgttctgtacatgggacaagtctttgttccggcaggtggaccttcaccggtccatacaMscIPciIEcoNIAflIIICas13d coreRxCas13d4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccacgcctatatcaacgacattgccgaggtcaattcctacttccaactgtaccattacatcatgcagagaattatcatgaatgagaggtacgagaaaagcagcggaaaggtgtccgagtacttcgacgctgtgaatgacgagaagaagtacaacgataggctcctgaaactgctgggtgcggatatagttgctgtaacggctccagttaaggatgaaggttgacatggtaatgtagtacgtctcttaatagtacttactctccatgctcttttcgtcgcctttccacaggctcatgaagctgcgacacttactgctcttcttcatgttgctatccgaggactttgacgacCas13d coreRxCas13d4,2204,2404,2604,2804,3004,3204,3404,360
    tgtgtgcctttcggctactgtatccccaggtttaagaacctgagcatcgaggccctgttcgataggaacgaggccgccaagttcgacaaggagaaaaagaaggtgtccGGCAATTCCGGATCCGGACTGTACCCTGAGCGGCTGCggcggatcctgaccgcggccgcttacccatacacacggaaagccgatgacataggggtccaaattcttggactcgtagctccgggacaagctatccttgctccggcggttcaagctgttcctctttttcttccacaggCCGTTAAGGCCTAGGCCTGACATGGGACTCGCCGACGccgcctaggactggcgccggcgaatgggtaEagINotIBamHIBamHIRxCas13dCas13d coreNESHA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    acgatgttccagattacgcttaatctagagggcccAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCtgctacaaggtctaatgcgaattagatctcccgggTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGApaIXbaIXcmIPspOMIWPREHA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    CTTGTATAAATCCTGGTtgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctaTTGCCACGGCGGAAGAACATATTTAGGACCAacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggatAACGGTGCCGCCTTWPRE4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTtgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgcTacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAacagccccttcgactgcaggaaaggtaccgacgagcggacacaacgAtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgccWPRE4,9204,9404,9604,9805,0005,0205,0405,060
    accttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgcTTCGAGCAGACATGATAAGATacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcgAAGCTCGTCTGTACTATTCTAtgtaactactcaaacctgtttggtgttgatcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgPsiISV40 late polyadenylation signalWPRE5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtaaaatcgataaggatcaGGGCTGGCGTAATAGCGAagaggcccgcaccgatcgcccttcccaacagttgcacgttatttgttcaattgttgttgttaacgtaagtaaaatacaaagtccaagtccccctctacaccctccaaaaaatttcgttcattttggagatgtttacaccattttagctattcctagtCCCGACCGCATTATCGCTtctccgggcgtggctagcgggaagggttgtcaacgClaIHpaIMfeIPvuIBsaBIBspDISV40 late polyadenylation signal5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcagcctgaatggcgaatggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcggcgtcggacttaccgcttacctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagccFspIphage f1 region5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcacccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagtAloIphage f1 region5,6205,6405,6605,6805,7005,7205,7405,760
    accctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgctgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaaggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgPsiIphage f1 region5,7805,8005,8205,8405,8605,8805,9005,9205,940
    atatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcactataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtgNdeIBsmBIEsp3I5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccgctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattggBsmBIEsp3I6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaagactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttMS2IQ4HF6RV8AL10IP12FF14AA16FC18LP20VF22AH24PE26TL28VK30VK32DA34ED36QL38GA40RV42GY44IE46beta-lactamaseampR6,3206,3406,3606,3806,4006,4206,4406,460
    ctggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtgAclIXmnILD48LN50SG52KI54LE56SF58RP60EE62RF64PM66MS68TF70KV72LL74CG76AV78LS80RI82DA84GQ86EQ88LG90RR92IH94YS96QN98DL100VE102YS104beta-lactamaseampR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacPvuIPV106TE108KH110LT112DG114MT116VR118EL120CS122AA124IT126MS128DN130TA132AN134LL136LT138TI140GG142PK144EL146TA148FL150HN152MG154DH156VT158RL160DR162Wbeta-lactamaseampR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagcAclIFspIE164PE166LN168EA170IP172ND174ER176DT178TM180PV182AM184AT186TL188RK190LL192TG194EL196LT198LA200SR202QQ204LI206DW208ME210AD212KV214AG216PL218LR220Sbeta-lactamaseampR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacAhdIBsaIA222LP224AG226WF228IA230DK232SG234AG236ER238GS240RG242II244AA246LG248PD250GK252PS254RI256VV258IY260TT262GS264QA266TM268DE270RN272RQ274IA276EI278Gbeta-lactamaseampR7,0207,0407,0607,0807,1007,1207,1407,160
    cctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatctA280SL282IK284HW286*beta-lactamaseampR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    aaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcac7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagca7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctcgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaBseYI7,7207,7407,7607,7807,8007,8207,8407,860
    ttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatggctcgacagatctaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtaccgagctgtctagaBglII7,8807,9007,9207,9407,9607,9808,0008,020
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    8030 bp6000650070007500500100015002000RxCas13dampRI2mRNApCN-reversebeta-lactamase

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    8030