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pKL2-233
  • Sequence Map
    Editing disabled on read-only sequence.
    ccatacaggtggATCCtaactcgagagtgcttttaactaagaattattagtcttttctgcttattttttcatcatagtttagaacactttatattaacgaatagtttatgaatctatttaggtttaaaaattgatacagttttataagttactttttcaaagactcgtgctgtctattggtatgtccaccTAGGattgagctctcacgaaaattgattcttaataatcagaaaagacgaataaaaaagtagtatcaaatcttgtgaaatataattgcttatcaaatacttagataaatccaaatttttaactatgtcaaaatattcaatgaaaaagtttctgagcacgacagataatENO2Zi...820406080100120140160
    gcataatgcactggaaggggaaaaaaaaggtgcacacgcgtggctttttcttgaatttgcagtttgaaaaatCCAAaccagatgtcaacacagctacAACGccttgccaacagggagttcttcagagacatggaggctcaaaacgaaattattgacagcctagacatcaatagtcatacgtattacgtgaccttccccttttttttccacgtgtgcgcaccgaaaaagaacttaaacgtcaaactttttaGGTTtggtctacagttgtgtcgatgTTGCggaacggttgtccctcaagaagtctctgtacctccgagttttgctttaataactgtcggatctgtagttatcagtatpTEF1tENO2ConL1 Scar180200220240260280300320340
    caacagaaagcgaccacccaactttggctgataatagcgtataaacaatgcatactttgtacgttcaaaatacaatgcagtagatatatttatgcatattacatataatacatatcacataggaagcaacaggcgcgttggacttttaattttcgaggaccgcgaatccttacatcacgttgtctttcgctggtgggttgaaaccgactattatcgcatatttgttacgtatgaaacatgcaagttttatgttacgtcatctatataaatacgtataatgtatattatgtatagtgtatccttcgttgtccgcgcaacctgaaaattaaaagctcctggcgcttaggaatgtagtgpTEF1360380400420440460480500520
    acccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtacttgggttagggggtgttcactagggggtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgapTEF1540560580600620640660680700
    aaaggtttggaaaagaaaaaagacaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcatcaatttctcaagtttcagtttccaaaccttttcttttttctgtggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcaattatttgccagtagttaaagagttcaaagtcpTEF1720740760780800820840860880
    tttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaaagatcTATGcctctgtttgagcttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatgttttctagATACggagacaaactcgaaaagtccgagtggtttcgattcgaacttagacgagttctgtccttgggaagaggtggacatctactBlpIpTEF19009209409609801,0001,0201,0401,060
    agttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttctagagctagagagattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgtcaacacctcgaccacacccttttaccagtctatagttgagtttcagtcagttcatctagctccttgtaaggaggtggtgttcgtttgagaagatctcgatctctctaacctttaccgagtttctgctgataccacctgctctagggatacagtcacggtagtgattactgcccaaacXbaIEcoRV1,0801,1001,1201,1401,1601,1801,2001,2201,240
    agtcaagacgatgactttgttccatggttgaatcatcatccctcccttgatggatattgctctgatttcttgcgtgatgtgtcgtctcctgttactgtcaacgagcaagagagtgatatggcggtaaaccaaactgctttcccgttgtttcagagaagaaaggatggcaatgaatcagtcagttctgctactgaaacaaggtaccaacttagtagtagggagggaactacctataacgagactaaagaacgcactacacagcagaggacaatgacagttgctcgttctctcactataccgccatttggtttgacgaaagggcaacaaagtctcttctttcctaccgttacttagtcBsmBIEsp3I1,2601,2801,3001,3201,3401,3601,3801,4001,420
    ctcctgctgcttcttcgtcgcagtataacggtttccaatcgcattctctgtatggaagtgatagagctagagatcttcctagccaacaaaccaatccggatcggtttactcagacgcaggaaccactaattactagtaacaagcctagtttggtcaacttttcacatttcttacgcccgaggacgacgaagaagcagcgtcatattgccaaaggttagcgtaagagacataccttcactatctcgatctctagaaggatcggttgtttggttaggcctagccaaatgagtctgcgtccttggtgattaatgatcattgttcggatcaaaccagttgaaaagtgtaaagaatgcgggSpeI1,4401,4601,4801,5001,5201,5401,5601,5801,600
    tgcaacttttgcgaagactactaataataaccttcatgacactaaagaaaagagtcctcaaagcccgccaaatgtgtttcagaccagagttcttggagctaaagactctgaagataaggttcttaacgagtctgttgcttctgctacgcctaaagataaccaaaaggcttgcctaataacgttgaaaacgcttctgatgattattattggaagtactgtgatttcttttctcaggagtttcgggcggtttacacaaagtctggtctcaagaacctcgatttctgagacttctattccaagaattgctcagacaacgaagacgatgcggatttctattggttttccgaacggattatBcgI1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tcagaggactcatgtagaaaagaccaagagagtgaaaaagcagttgtatgttcttctgttggctcgggtaatagtctcgatggcccatccgaaagtccttcactttctttaaagagaaagcattcgaatattcaagacattgactgtcatagtgaagatgtggaagaagaatcaggagagtctcctgagtacatcttttctggttctctcactttttcgtcaacatacaagaagacaaccgagcccattatcagagctaccgggtaggctttcaggaagtgaaagaaatttctctttcgtaagcttataagttctgtaactgacagtatcacttctacaccttcttcttagtcctcBstBI1,8001,8201,8401,8601,8801,9001,9201,940
    atggaagaaaggaagcaggtccatctcgaacgggtttgggttcaaagagaagccgctctgcagaagtgcataatctgtctgaaaggagacggcgtgataggatcaacgagaagatgcgtgccctgcaagaactcattccaaactgtaacaaggtggacaaagcttcgatgctagatgataccttctttccttcgtccaggtagagcttgcccaaacccaagtttctcttcggcgagacgtcttcacgtattagacagactttcctctgccgcactatcctagttgctcttctacgcacgggacgttcttgagtaaggtttgacattgttccacctgtttcgaagctacgatctactPstIBsmBIEsp3I1,9601,9802,0002,0202,0402,0602,0802,1002,120
    agccatcgagtatctcaagtcactccaacttcaagtgcagatcatgtcaatggcgtctggttactatctgccaccggcggttatgttcccaccgggtatggggcattacccggcagcagctgctgcaatggcaatgggtatgggaatgccttatgcaatgggcttgcctgatttgagctcggtagctcatagagttcagtgaggttgaagttcacgtctagtacagttaccgcagaccaatgatagacggtggccgccaatacaagggtggcccataccccgtaatgggccgtcgtcgacgacgttaccgttacccatacccttacggaatacgttacccgaacggactaaactcgSgrAI2,1402,1602,1802,2002,2202,2402,2602,2802,300
    cgtggtggttcatcggttaaccacggaccacagttccaagtctcggggatgcaacaacaaccagtggcgatgggtattccacgtgtctctggtggtggtatctttgccggttcttcgacgattggcaatggctcgactagagatttatctggttctaaagatcaaacaacgacgaatagcaccaccaagtagccaattggtgcctggtgtcaaggttcagagcccctacgttgttgttggtcaccgctacccataaggtgcacagagaccaccaccatagaaacggccaagaagctgctaaccgttaccgagctgatctctaaatagaccaagatttctagtttgttgctgcttatHpaIPmlIBsaAI2,3202,3402,3602,3802,4002,4202,4402,4602,480
    acaacagtaacttgaaaccaataaagagaaaacaggggtcttctgatcagttttgtggatcgtcgcccaagaagaagaggaaagtcggATCCgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatgttgtcattgaactttggttatttctcttttgtccccagaagactagtcaaaacacctagcagcgggttcttcttctcctttcagccTAGGcgggggggctggctacagtcggaccccctgctcgaggtgaatctgccgctcctgcaccgctaccgcgtacggctgcgcgatctgctBclIBmgBIPshAIVP162,5002,5202,5402,5602,5802,6002,6202,6402,660
    tttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtaactcgagTGGCagtgcttttaactaagaaaagctagacctgtacaaccccctgcccctaaggggccccggccctaaatggggggtgctgaggcgggggatgccgcgagacctataccggctgaagctcaaactcgtctacaaatggctacgggaaccttaactgctcatgccacccattgagctcACCGtcacgaaaattgattctSmaIXmaIBsaBIPspXITspMIVP16tENO22,6802,7002,7202,7402,7602,7802,8002,8202,840
    attattagtcttttctgcttattttttcatcatagtttagaacactttatattaacgaatagtttatgaatctatttaggtttaaaaattgatacagttttataagttactttttcaaagactcgtgctgtctattgcataatgcactggaaggggaaaaaaaaggtgcacacgcgtgtaataatcagaaaagacgaataaaaaagtagtatcaaatcttgtgaaatataattgcttatcaaatacttagataaatccaaatttttaactatgtcaaaatattcaatgaaaaagtttctgagcacgacagataacgtattacgtgaccttccccttttttttccacgtgtgcgcactENO22,8602,8802,9002,9202,9402,9602,9803,0003,020
    gctttttcttgaatttgcagtttgaaaaatGATGcacacactggcttaagatgacAACGggtaccgggcccttatattgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatggcattaccaccatatacatatccatatctaatctcgaaaaagaacttaaacgtcaaactttttaCTACgtgtgtgaccgaattctactgTTGCccatggcccgggaatataacttaaaagtttttaagaatgaaaaaaaaacctacctgcgtttcttcaaattattagtataatgtaccgtaatggtggtatatgtataggtatagattagaApaIKpnIAflIIAcc65IPspOMIEcoO109IpGal1-mut-5'tENO2ConL2 Scar3,0403,0603,0803,1003,1203,1403,1603,1803,200
    tacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtgaattcgGCGTGGGCGtGCGTGGGCGgGCGTGGGCGtGCGTGGGCGgGCGTGGGCGtGCGTGGGCGggatcatgaatatacaacacctttacatttctcggggtaatagaatcggatttttttggaagagaaaccttgaaagtcattatgcgaattgacgagtaacgatataacttcacttaagcCGCACCCGCaCGCACCCGCcCGCACCCGCaCGCACCCGCcCGCACCCGCaCGCACCCGCcctagEcoRIpGal1-mut-5'3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctaatgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggagggcacgcaggagcagaagtggccagcgcaaggactttgcgtctacacggattacggcgtgacgaggcttgttatttctaagatgttatgatcgaaaataccaatacttctcctttttaaccgtcattggaccggggtgtttggaagtttaattgcttagtttaattgttggtatcctpGal1-mut-3'3,4003,4203,4403,4603,4803,5003,5203,5403,560
    tgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaactattacgctaatcaaaaaatcggaataaagaccccattaattagtcgcttcgctactaaaaactagataattgtctatatatttaccttttcgacgtattggtgaaattgattatgaaagttgtaaaagtcaaacataatgaagaataagtttacagtattttcatagttgtttttpGal1-mut-3'Mi...S3,5803,6003,6203,6403,6603,6803,7003,720
    attgttaatatacctctatactttaacgtcaaggagaaaaaactatactcgagagatcTATGgtgtccaaaggagaggagttaatcaaggaaaacatgagaatgaaagttgtcatggagggctccgttaatggtcaccaattcaagtgtacaggggaaggtgaaggtaatccttacattaacaattatatggagatatgaaattgcagttcctcttttttgatatgagctctctagATACcacaggtttcctctcctcaattagttccttttgtactcttactttcaacagtacctcccgaggcaattaccagtggttaagttcacatgtccccttccacttccattaggaatgtaBstEIImRuby2 (Gene Comp.)mRuby2pGal1-mut-3'3,7403,7603,7803,8003,8203,8403,8603,8803,900
    gggtacacaaactatgagaattaaagtaattgaaggcggaccactaccatttgcatttgacattctggcaacgtcattcatgtacggatcacgaactttcatcaagtaccctaaaggtataccagactttttcaagcaatcttttccagagggttttacatgggaaagggttacaagacccatgtgtttgatactcttaatttcattaacttccgcctggtgatggtaaacgtaaactgtaagaccgttgcagtaagtacatgcctagtgcttgaaagtagttcatgggatttccatatggtctgaaaaagttcgttagaaaaggtctcccaaaatgtaccctttcccaatgttctBstZ17ImRuby2 (Gene Comp.)mRuby23,9203,9403,9603,9804,0004,0204,0404,0604,080
    tacgaagatgggggtgtcgtcacagttatgcaagatacttcattagaagatggctgccttgtctatcatgtgcaagtaagaggggtgaattttccttctaacggacctgtgatgcagaaaaagaccaaaggttgggaaccaaatactgaaatgatgtacccagctgatggaggtttgaatgcttctacccccacagcagtgtcaatacgttctatgaagtaatcttctaccgacggaacagatagtacacgttcattctccccacttaaaaggaagattgcctggacactacgtctttttctggtttccaacccttggtttatgactttactacatgggtcgactacctccaaactmRuby2 (Gene Comp.)mRuby24,1004,1204,1404,1604,1804,2004,2204,2404,260
    gaggctacacacacatggcgcttaaagttgatggtggaggtcatttgtcttgtagttttgttaccacttatcgttctaaaaagactgttggcaatatcaaaatgccaggaatacatgctgtagaccacagactagaaagactcgaagagagcgataacgaaatgttcgttgtacagagctccgatgtgtgtgtaccgcgaatttcaactaccacctccagtaaacagaacatcaaaacaatggtgaatagcaagatttttctgacaaccgttatagttttacggtccttatgtacgacatctggtgtctgatctttctgagcttctctcgctattgctttacaagcaacatgtctcmRuby2 (Gene Comp.)mRuby24,2804,3004,3204,3404,3604,3804,4004,4204,440
    agagcatgccgtagccaaatttgctggcttaggcggtggtatggatgaattgtataagggATCCtaactcgagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaattctcgtacggcatcggtttaaacgaccgaatccgccaccatacctacttaacatattcccTAGGattgagctctaggcgagattggcttttccttcctcaatctgttggacttcagatccagggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttaAclIBplIBplIPpuMIEcoO109ICyc1 TerminatormRuby2 (Gene Comp.)mRuby24,4604,4804,5004,5204,5404,5604,5804,6004,620
    ttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagAGCAgttacagagatgttacgaaccactagtgcactgcagTACAgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggaaaagaaaaaaaagacatgtctgcgcacatgcgtacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttcTCGTcaatgtctctacaatgcttggtgatcacgtgacgtcATGTcaaatcgaacggagcaggggcggcccagtgggccggtcgctgtacctccBarIPstISapISpeIBspQIBstEIICyc1 TerminatorHygromycinR (Yeast Marker)AgTEF promoterConRE' Scar4,6404,6604,6804,7004,7204,7404,7604,7804,800
    cccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctccagacctgcgagcagggaaagggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatcgggtatgtaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcgtttttaatgccgaggagcgaggtctggacgctcgtccctttFspIBmgBIHygromycinR (Yeast Marker)AgTEF promoter4,8204,8404,8604,8804,9004,9204,9404,9604,980
    cgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaaaatgggtaaaaagcctgaactcagcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaaacggtgactccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgtagtgtaggcttgtatttgtttttacccatttttcggacttgagtHygromycinR (Yeast Marker)AgTEF promoterHygromycinR5,0005,0205,0405,0605,0805,1005,1205,1405,160
    ccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtgtccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcaggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcacaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtAhdIZraIAatIIPshAIHygromycinR (Yeast Marker)HygromycinR5,1805,2005,2205,2405,2605,2805,3005,3205,340
    ctttgcatcggccgcgctcccgattccggaagtgcttgacattggggaatttagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcaaccggtcgcggaggccatggatgcgatcgctgcggccgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaaatcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgttggccagcgcctccggtacctacgctagcgacgccggHygromycinR (Yeast Marker)HygromycinR5,3605,3805,4005,4205,4405,4605,4805,500
    gatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccNdeIPflFIRsrIITth111IHygromycinR (Yeast Marker)HygromycinR5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcaggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtNmeAIIIHygromycinR (Yeast Marker)HygromycinR5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgaSacIIHygromycinR (Yeast Marker)HygromycinR5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataaagtaactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatcagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatttcattgactgttatttttctaagaacaaaagttcttgaacagtaaacatatcaaaaaaataScaIHygromycinR (Yeast Marker)HygromycinRAgTEF terminator6,0606,0806,1006,1206,1406,1606,1806,2006,220
    attgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgGAGTtatcgtgttgcatctgcggctttaaattgatgttaacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatagtacgcagttagcatacacttacgaccagcgatatgacCTCAatagcacaacgtagacgccgaaatttaactacaFspIHygromycinR (Yeast Marker)AgTEF terminatorHO 3' Homology (Yeast Origin)HO - 3' Homology6,2406,2606,2806,3006,3206,3406,3606,3806,400
    atctcatcgcaggcacgggcagtacagtgccctgagcgtagggaaaaatgaaaaaaaggatgtaacttttaacataattccagcacgcagcgattgggtataatgaagattgttaagttcaacaacatgtgaaattcttagctccgcaacttgcttgaattatgagctctaagattcatagagtagcgtccgtgcccgtcatgtcacgggactcgcatccctttttactttttttcctacattgaaaattgtattaaggtcgtgcgtcgctaacccatattacttctaacaattcaagttgttgtacactttaagaatcgaggcgttgaacgaacttaatactcgagattctaagtHO 3' Homology (Yeast Origin)HO - 3' Homology6,4206,4406,4606,4806,5006,5206,5406,5606,580
    agaagtaaataggtcatataatggaatgatagtagtacttgataaaggaaccatgtgatcttacgttgatatgaaaataattctctcacagaagtcagtttgtatgactaacatagagagtaattgcttttcggataaaatcgcacctaacaaagattaccttttgtaaagcctccagtcttcatttatccagtatattaccttactatcatcatgaactatttccttggtacactagaatgcaactatacttttattaagagagtgtcttcagtcaaacatactgattgtatctctcattaacgaaaagcctattttagcgtggattgtttctaatggaaaacatttcggaggtcScaIHO 3' Homology (Yeast Origin)HO - 3' Homology6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aacagctatgaatgttcgttattctacgcactttctttcatacatcttgatccgccttttttgatgtaggttttaacgcggcaaaacatattaatttttctgatttggaaaaataaagagttagttctgccCCGAgcggccgcgtgttacaaccaattaaccaattctgattagaaaattgtcgatacttacaagcaataagatgcgtgaaagaaagtatgtagaactaggcggaaaaaactacatccaaaattgcgccgttttgtataattaaaaagactaaacctttttatttctcaatcaagacggGGCTcgccggcgcacaatgttggttaattggttaagactaatcttttNotIHO 3' Homology (Yeast Origin)HO - 3' HomologyKanR Terminator6,7806,8006,8206,8406,8606,8806,9006,9206,940
    actcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattKanR6,9606,9807,0007,0207,0407,0607,0807,1007,120
    tttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgccaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcgaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggKanR7,1407,1607,1807,2007,2207,2407,2607,280
    tgagcgaggcgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatactcgctccgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtaSmaIXmaITspMIKanR7,3007,3207,3407,3607,3807,4007,4207,4407,460
    caggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacgtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgClaIBspDIKanR7,4807,5007,5207,5407,5607,5807,6007,6207,640
    attatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacattaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggacctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactactatataaaaatagaacacgttacattgtaNruIKanRKanR Promoter7,6607,6807,7007,7207,7407,7607,7807,8007,820
    cagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagtcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgtctctaaaactctgtgttgcaccgaaacaacttatttagcttgaaaacgactcaacttcctagtcagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaaColE1 originColE1KanR Promoter7,8407,8607,8807,9007,9207,9407,9607,9808,000
    gcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctcgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgaColE1 originColE18,0208,0408,0608,0808,1008,1208,1408,1608,180
    ctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcgacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgColE1 originColE18,2008,2208,2408,2608,2808,3008,3208,3408,360
    tatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccColE1 originColE18,3808,4008,4208,4408,4608,4808,5008,5208,540
    ggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtgcggccgcCAATtctaattgtatcgagatcacttttcgtgatccgctaatcagcgacggtccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcacgccggcgGTTAagattaacatagctctagtgaaaagcactaggcgattagtcgctgccaNotIColE1HO 5' Homology (Yeast Origin)HO - 5' HomologyColE1 origin8,5608,5808,6008,6208,6408,6608,6808,7008,720
    cacattaggtttgccaagtcagggtatgaaccatacgatcagttttcgtgaacctggtacgtatattgtggcgtttgtgtatattttcattctttgacaacaatcaataccaacctcaaataggaaaagtaataagtttggcgttacaccccaaaagacgccaaacggatcgaacttagtgtaatccaaacggttcagtcccatacttggtatgctagtcaaaagcacttggaccatgcatataacaccgcaaacacatataaaagtaagaaactgttgttagttatggttggagtttatccttttcattattcaaaccgcaatgtggggttttctgcggtttgcctagcttgaatBsaAISexAISnaBIHO 5' Homology (Yeast Origin)HO - 5' Homology8,7408,7608,7808,8008,8208,8408,8608,8808,900
    ctcaatagcaattagcgagacaaaacctacgttaagacctgtaaccgatttatcaaagcactctgcggttctttcttgggaatattacctggacattttgtgccctcaagaaacgaggctctacgagcctgttggagcccctcagacattagccgccacgaatcaaactttttacgcggagttatcgttaatcgctctgttttggatgcaattctggacattggctaaatagtttcgtgagacgccaagaaagaacccttataatggacctgtaaaacacgggagttctttgctccgagatgctcggacaacctcggggagtctgtaatcggcggtgcttagtttgaaaaatgcgcHO 5' Homology (Yeast Origin)HO - 5' Homology8,9208,9408,9608,9809,0009,0209,0409,060
    attcggcccaaatcagtttctcacagatcattcgtagagtgaaaaagcacatcgattatttgatacccctttgggttaattactgttgaggtctttgccgtaagttccatcgctgtCCCTgaattcgcatctagaCTGAactggccgataattgcagacgAACGccttgccaacagggtaagccgggtttagtcaaagagtgtctagtaagcatctcactttttcgtgtagctaataaactatggggaaacccaattaatgacaactccagaaacggcattcaaggtagcgacaGGGActtaagcgtagatctGACTtgaccggctattaacgtctgcTTGCggaacggttgtcccBcgIClaIXbaIBspDIEcoRIHO 5' Homology (Yeast Origin)HO - 5' HomologyConLS ScarsgRNA-HO-2pTEF19,0809,1009,1209,1409,1609,1809,2009,2209,240
    agttcttcagagacatggaggctcaaaacgaaattattgacagcctagacatcaatagtcatacaacagaaagcgaccacccaactttggctgataatagcgtataaacaatgcatactttgtacgttcaaaatacaatgcagtagatatatttatgcatattacatataatacatattcaagaagtctctgtacctccgagttttgctttaataactgtcggatctgtagttatcagtatgttgtctttcgctggtgggttgaaaccgactattatcgcatatttgttacgtatgaaacatgcaagttttatgttacgtcatctatataaatacgtataatgtatattatgtatapTEF19,2609,2809,3009,3209,3409,3609,3809,4009,420
    cacataggaagcaacaggcgcgttggacttttaattttcgaggaccgcgaatccttacatcacacccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacgtgtatccttcgttgtccgcgcaacctgaaaattaaaagctcctggcgcttaggaatgtagtgtgggttagggggtgttcactagggggtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgpTEF19,4409,4609,4809,5009,5209,5409,5609,5809,600
    ccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagacaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttcggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctgtggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagpTEF19,6209,6409,6609,6809,7009,7209,7409,7609,780
    tctttcgatgacctcccattgatatttaagttaataaacggtcatcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaaagatcTATGatggtttccggagtcggagaaagctactggagggtaactataaattcaattatttgccagtagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatgttttctagATACtaccaaaggcctcagccpTEF19,8009,8209,8409,8609,8809,9009,9209,9409,960
    gggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcacccatcaccgccaccggcaccgccaccggcaccgcctcttcttcttggcagcagttcagtgtgaggattattggcttctcctcctcttgttcgagttagcagcccttgctttagagagtctggttcttcgttgtgacttagttactcgtttcgttaagttgtcatgtggcagctgcgtSalISgrDI9,98010,00010,02010,04010,06010,08010,10010,12010,140
    agactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgtctgaggtgcggcaaaagcttgttaggccgcttagtccctttagtaagctgatgagtgttagtgagttttgctgctgcatgccaaggagacatggactcgttgtctagtgtcgaatagagagagcttaggtcgctccaccaatgtaagtcggaaagcctacatactagcggcagctacBsiWIBstBI10,16010,18010,20010,22010,24010,26010,28010,30010,320
    aatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagatttaggtcaaaggcctagtagccaatgtcacttttgcggtctctttacaatccctaatacggagttagacaaggatgagaactctttggactctaagatcgatacccttgactacactctagaaacaagtgaagaagctcgagctaagatgagctcgcacgaaagcaacgagctctctaPspXI10,34010,36010,38010,40010,42010,44010,46010,48010,500
    taccttgttaaatccggtttggatacattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctatggaacaatttaggccaaacctatgtaaggttcttatgaccatttggcaaaatgcggtaagaagtatcctaactacaaccacaacaataactaaatctcggtcgatcttgacttctaggacgcgaaagataacgaccacgacaagttagcgtctttgagcgccaagcacgctaaagaAloI10,52010,54010,56010,58010,60010,62010,64010,66010,680
    cagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagatgatttagagccttatattggactgcattgtcaatgtccgagaaggaccacctctataattcgaaaacacactgtgacagcacctttcacactccctgaactgaccaatactagcacaataccaaatattcaaagtacttctactcgtacctcttcaacatcgactctcatttgctctactaaatctcggaatataacctgacgtaaSapIBspQIPflFITth111I10,70010,72010,74010,76010,78010,80010,82010,840
    atcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatattaggacgatgactataaggagttcgcagttccaagaacaaattcgtcttggcacaggcttactatcatctaacgttacggtgtggacaagaacaccaggtcctgctatccgattgagtcagatacacgaaccaaccaagatgagaatcccgaggagtaccaacagtgagagtcatataPfoI10,86010,88010,90010,92010,94010,96010,98011,00011,020
    ggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgccgattgtaccctagataacgcagaaatcgttaccgccaatattagttacctttacttctactaccctcgttacatcgatcaccttcttcgagctactccgaaaccccaaaccaacaaacggtagtgtgaagaagagcgacgtatggcaaaggcgattccatacgaacactcaaaaac11,04011,06011,08011,10011,12011,14011,16011,18011,200
    atgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcattacgtccgaaagccaaatgtcaatttgtaccttaacgtcaatcgaaacgtttacagtctctttgcgcaaaactcttgcgtctgtgacaatacactatacgaagacgcactgagcggacgaccttaacaatgtgtctcagggtcatagtacctgaatcactttacactgccacgtcgtaBmrI11,22011,24011,26011,28011,30011,32011,34011,36011,380
    ttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcaagaaatggtgcccttcataatgggcaacccacaacgaggatcacttcaagtctattttctacaacacctcaccaacgaacgcttagtacgcctaagttggcctaattcgtgactatcaaatccgctacgccccatagggccacgtcgacgcaatcccctacgacacacgccataccg11,40011,42011,44011,46011,48011,50011,52011,54011,560
    agttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcaaccatggtcaacgtatatagtgtttttctctgaaagaaaaaaccaaagctagagtgtgacgctttctttagtttacccctccgcgattcgtagtaggcctcctatttctactacccgttgcttacgtaggagcaagcaggaaagtccgaaaagaacttcaacaattctcggcctcagttggtaccBlpI11,58011,60011,62011,64011,66011,68011,70011,72011,740
    gaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctggTTCTggtacccgcccatatgcttgccctgtcgagtcctgcgatcgccgcttttctcgctcggatgagcttacccgccatatccgcatccataccggtcagaagcccttccctttgacgcctttacctacgctaagtgagcgaggtcgaataagactctctgagaaaatttcttagaccAAGAccatgggcgggtatacgaacgggacagctcaggacgctagcggcgaaaagagcgagcctactcgaatgggcggtataggcgtaggtatggccagtcttcgggaaggKpnINdeIAcc65IZif26811,76011,78011,80011,82011,84011,86011,88011,90011,920
    agtgtcgaatctgcatgcgtaacttcagtcgtagtgaccaccttaccacccacatccgcacccacacaggcgagaagccttttgcctgtgacatttgtgggaggaagtttgccaggagtgatgaacgcaagaggcataccaaaattcacagcttagacgtacgcattgaagtcagcatcactggtggaatggtgggtgtaggcgtgggtgtgtccgctcttcggaaaacggacactgtaaacaccctccttcaaacggtcctcactacttgcgttctccgtatggttttaZif26811,94011,96011,98012,00012,02012,04012,060
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12071 bp90001000011000100020003000HO - 5' HomologytENO2sgRNA-HO-2pTEF1o56 Reverse Primer (DNA)tENO2,ConL1 Scar,pTEF1BlpIBsmBI,EcoRV,XbaI,BsmBI,Esp3ISpeIBcgIBsmBI,BstBI,PstI,BsmBI,Esp3ISgrAIHpaI,BsaAI,PmlIBclI,PshAI,BmgBIBsaBIVP16TspMI,XmaI,SmaI,PspXIHO 5' Homology (Yeast Origin)BspDIClaIEcoRIXbaIConLS Scar,BcgISalI,SgrDI,BstBIBsiWIPspXI,AloIBspQI,SapI,PflFI,Tth111I,PfoIBmrIZif268,BlpI,Acc65I,KpnI,NdeI

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    12071