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pKL1-391
  • Sequence Map
    Editing disabled on read-only sequence.
    tatcagttattacccGAGTagagcacttgaatccactgccccgggaatctcggtcgtaatgatttctataatgacgaaaaaaaaaaaattggaaagaaaaagcttcatggcctttataaaaaggaactatccaatacctcgccagaaccaagtaacagtattttacggggcacaaatagtcaataatgggCTCAtctcgtgaacttaggtgacggggcccttagagccagcattactaaagatattactgcttttttttttttaacctttctttttcgaagtaccggaaatatttttccttgataggttatggagcggtcttggttcattgtcataaaatgccccgtgttSmaIXmaITspMIURA3 - 3' HomologyURA3...tor20406080100120140160
    atcaagaacaataagacaggactgtaaagatggacgcattgaactccaaagaacaacaagagttccaaaaagtagtggaacaaaagcaaatgaaggatttcatgcgtttgtactctaatctggtagaaagatgtttcacagactgtgtcaatgacttcacaacatcaaagctaactagttcttgttattctgtcctgacatttctacctgcgtaacttgaggtttcttgttgttctcaaggtttttcatcaccttgttttcgtttacttcctaaagtacgcaaacatgagattagaccatctttctacaaagtgtctgacacagttactgaagtgttgtagtttcgattgPflFITth111IURA3 - 3' Homology180200220240260280300320340
    caataaggaacaaacatgcatcatgaagtgctcagaaaagttcttgaagcatagcgaacgtgtagggcagcgtttccaagaacaaaacgctgccttgggacaaggcttgggccgataaggtgtactggcgtatatatatctaattatgtatctctggtgtagcccatttttagcagttattccttgtttgtacgtagtacttcacgagtcttttcaagaacttcgtatcgcttgcacatcccgtcgcaaaggttcttgttttgcgacggaaccctgttccgaacccggctattccacatgaccgcatatatatagattaatacatagagaccacatcgggtaaaaatcgtURA3 - 3' Homology360380400420440460480500520
    tgtaaatataaagaCCGAgcggccgcgattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcaacatttatatttctGGCTcgccggcgctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtBanIEagINotIAmpR-ColE1 (Bac. Marker/Origin)AmpR terminatorAmpRURA3...ogy540560580600620640660680700
    tccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgccctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcAhdIBglIKflIPpuMISacIINmeAIIIEcoO109IAmpR-ColE1 (Bac. Marker/Origin)AmpR720740760780800820840860
    cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtaAseIFspIAmpR-ColE1 (Bac. Marker/Origin)AmpR8809009209409609801,0001,0201,040
    gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagcaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcBcgIPvuIScaIAmpR-ColE1 (Bac. Marker/Origin)AmpR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactaBsaHIAmpR-ColE1 (Bac. Marker/Origin)AmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaactSspIBciVIAmpR-ColE1 (Bac. Marker/Origin)AmpRAmpR promoter1,4201,4401,4601,4801,5001,5201,5401,560
    atgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgtcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgtacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE1AmpR promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcggtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE11,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcggagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcBseYIAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE11,9401,9601,9802,0002,0202,0402,0602,0802,100
    tgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgDrdIBciVIHaeIIAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE12,1202,1402,1602,1802,2002,2202,2402,260
    tcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtgcggccgcCAATgctaaattcgagtgaaacacaggaagatcagaaaatcagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcacgccggcgGTTAcgatttaagctcactttgtgtccttctagtcttttagAjuIEagINotIAmpR-ColE1 (Bac. Marker/Origin)ColE1ColE1 originURA3 - 5' Homology2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctcatttcatccatattaacaataatttcaaatgtttatttgcattatttgaaactaggcaagacaagcaacgaaacgtttttgaaaattttgagtattttcaataaatttgtagaggactcagatattgaaaaaaagctacagcaattaatacttgataagaagagtattgagagagtaaagtaggtataattgttattaaagtttacaaataaacgtaataaactttgatccgttctgttcgttgctttgcaaaaacttttaaaactcataaaagttatttaaacatctcctgagtctataactttttttcgatgtcgttaattatgaactattcttctcataactctAseIURA3 - 5' Homology2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agggcaacggttcatcatctcatggatctgcacatgaacaaacaccagagtcaaacgacgttgaaattgaggctactgcgccaattgatgacaatacagacgatgataacaaaccgaagttatctgatgtagaaaaggattaaagatgctaagagatagtgatgatatttcataatcccgttgccaagtagtagagtacctagacgtgtacttgtttgtggtctcagtttgctgcaactttaactccgatgacgcggttaactactgttatgtctgctactattgtttggcttcaatagactacatcttttcctaatttctacgattctctatcactactataaagtattMfeIURA3 - 5' Homology2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ataatgtaattctatatatgttaattaccttttttgcgaggcatatttatggtgaaggataagttttgaccatcaaagaaggttaatgtggctgtggtttcagggtccataaagcccacatggataacattacCCCTgaattcgcatctagaacggggtcatcacggctcatcattattacattaagatatatacaattaatggaaaaaacgctccgtataaataccacttcctattcaaaactggtagtttcttccaattacaccgacaccaaagtcccaggtatttcgggtgtacctattgtaatgGGGActtaagcgtagatcttgccccagtagtgccgagtagtaXbaIEcoRIURA3 - 5' HomologyConL1ConR12,8202,8402,8602,8802,9002,9202,9402,960
    gcgccaaacaaatgtgtgcaatacacgctcggatgactgcatgatgaccgcactgactggggacagcagatccacctaagcctgtgagagaagcagacacccgacagatcaaggcagttacgtctcaccaaaccagatgtcaacacagctacAACGccttgccaacagggagttccgcggtttgtttacacacgttatgtgcgagcctactgacgtactactggcgtgactgacccctgtcgtctaggtggattcggacactctcttcgtctgtgggctgtctagttccgtcaatgcagagtggtttggtctacagttgtgtcgatgTTGCggaacggttgtccctcaagBpu10IConL1ConR1ConL1 ScarpTEF12,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttcagagacatggaggctcaaaacgaaattattgacagcctagacatcaatagtcatacaacagaaagcgaccacccaactttggctgataatagcgtataaacaatgcatactttgtacgttcaaaatacaatgcagtagatatatttatgcatattacatataatacatatcaaagtctctgtacctccgagttttgctttaataactgtcggatctgtagttatcagtatgttgtctttcgctggtgggttgaaaccgactattatcgcatatttgttacgtatgaaacatgcaagttttatgttacgtcatctatataaatacgtataatgtatattatgtatagtAjuIpTEF13,1603,1803,2003,2203,2403,2603,2803,3003,320
    cataggaagcaacaggcgcgttggacttttaattttcgaggaccgcgaatccttacatcacacccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacagtatccttcgttgtccgcgcaacctgaaaattaaaagctcctggcgcttaggaatgtagtgtgggttagggggtgttcactagggggtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtpTEF13,3403,3603,3803,4003,4203,4403,4603,4803,500
    cccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagacaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgattttgggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctgtggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaapTEF13,5203,5403,5603,5803,6003,6203,6403,660
    tttctctttcgatgacctcccattgatatttaagttaataaacggtcatcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaaagatcTATGcctctgtttgaaagagaaagctactggagggtaactataaattcaattatttgccagtagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatgttttctagATACggagacaaacBglIIpTEF13,6803,7003,7203,7403,7603,7803,8003,8203,840
    agcttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttctagagctagagatcgaaaagtccgagtggtttcgattcgaacttagacgagttctgtccttgggaagaggtggacatctacttcaacacctcgaccacacccttttaccagtctatagttgagtttcagtcagttcatctagctccttgtaaggaggtggtgttcgtttgagaagatctcgatctctBlpIXbaIBsaXIEcoRV3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcatccctcccttgatggatattgctctgatttcttgcgtgatgtgtcgtctcctgttactgtcaacctaacctttaccgagtttctgctgataccacctgctctagggatacagtcacggtagtgattactgcccaaactcagttctgctactgaaacaaggtaccaacttagtagtagggagggaactacctataacgagactaaagaacgcactacacagcagaggacaatgacagttgNcoI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gagcaagagagtgatatggcggtaaaccaaactgctttcccgttgtttcagagaagaaaggatggcaatgaatcagctcctgctgcttcttcgtcgcagtataacggtttccaatcgcattctctgtatggaagtgatagagctagagatcttcctagccaacaaaccaatccggctcgttctctcactataccgccatttggtttgacgaaagggcaacaaagtctcttctttcctaccgttacttagtcgaggacgacgaagaagcagcgtcatattgccaaaggttagcgtaagagacataccttcactatctcgatctctagaaggatcggttgtttggttaggccBglIIBspEI4,2204,2404,2604,2804,3004,3204,3404,360
    atcggtttactcagacgcaggaaccactaattactagtaacaagcctagtttggtcaacttttcacatttcttacgccctgcaacttttgcgaagactactaataataaccttcatgacactaaagaaaagagtcctcaaagcccgccaaatgtgtttcagaccagagttcttggtagccaaatgagtctgcgtccttggtgattaatgatcattgttcggatcaaaccagttgaaaagtgtaaagaatgcgggacgttgaaaacgcttctgatgattattattggaagtactgtgatttcttttctcaggagtttcgggcggtttacacaaagtctggtctcaagaaccBbsISpeI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agctaaagactctgaagataaggttcttaacgagtctgttgcttctgctacgcctaaagataaccaaaaggcttgcctaatatcagaggactcatgtagaaaagaccaagagagtgaaaaagcagttgtatgttcttctgttggctcgggtaatagtctcgatggcccatccgaatcgatttctgagacttctattccaagaattgctcagacaacgaagacgatgcggatttctattggttttccgaacggattatagtctcctgagtacatcttttctggttctctcactttttcgtcaacatacaagaagacaaccgagcccattatcagagctaccgggtaggcttBcgI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agtccttcactttctttaaagagaaagcattcgaatattcaagacattgactgtcatagtgaagatgtggaagaagaatcaggagatggaagaaaggaagcaggtccatctcgaacgggtttgggttcaaagagaagccgctctgcagaagtgcataatctgtctgaaaggagactcaggaagtgaaagaaatttctctttcgtaagcttataagttctgtaactgacagtatcacttctacaccttcttcttagtcctctaccttctttccttcgtccaggtagagcttgcccaaacccaagtttctcttcggcgagacgtcttcacgtattagacagactttcctctgPstISspIBfuAIBspMIBstBI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggcgtgataggatcaacgagaagatgcgtgccctgcaagaactcattccaaactgtaacaaggtggacaaagcttcgatgctagatgaagccatcgagtatctcaagtcactccaacttcaagtgcagatcatgtcaatggcgtctggttactatctgccaccggcggttatgttccgcactatcctagttgctcttctacgcacgggacgttcttgagtaaggtttgacattgttccacctgtttcgaagctacgatctacttcggtagctcatagagttcagtgaggttgaagttcacgtctagtacagttaccgcagaccaatgatagacggtggccgccaatacaaBsaHIBsaXISgrAI4,9204,9404,9604,9805,0005,0205,0405,060
    cccaccgggtatggggcattacccggcagcagctgctgcaatggcaatgggtatgggaatgccttatgcaatgggcttgcctgatttgagccgtggtggttcatcggttaaccacggaccacagttccaagtctcggggatgcaacaacaaccagtggcgatgggtattccacgtgggtggcccataccccgtaatgggccgtcgtcgacgacgttaccgttacccatacccttacggaatacgttacccgaacggactaaactcggcaccaccaagtagccaattggtgcctggtgtcaaggttcagagcccctacgttgttgttggtcaccgctacccataaggtgcaHpaIPmlIBsaAIPvuII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gtctctggtggtggtatctttgccggttcttcgacgattggcaatggctcgactagagatttatctggttctaaagatcaaacaacgacgaataacaacagtaacttgaaaccaataaagagaaaacaggggtcttctgatcagttttgtggatcgtcgcccaagaagaagaggacagagaccaccaccatagaaacggccaagaagctgctaaccgttaccgagctgatctctaaatagaccaagatttctagtttgttgctgcttattgttgtcattgaactttggttatttctcttttgtccccagaagactagtcaaaacacctagcagcgggttcttcttctcctBbsIBclI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aagtcggATCCgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgcttcagccTAGGcgggggggctggctacagtcggaccccctgctcgaggtgaatctgccgctcctgcaccgctaccgcgtacggctgcgcgatctgctaaagctagacctgtacaaccccctgcccctaaggggccccggccctaaatggggggtgctgaggcgggggatgccgcgSacISmaISphIXmaIBamHIBmgBIBsaBIPshAITspMIEco53kIVP165,4405,4605,4805,5005,5205,5405,5605,5805,600
    tctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtaactcgagTGGCagtgcttttaactaagaattattagtcttttctgcttattttttcatcatagtttagaacactttatattaacgaatagtttatgaatctatagacctataccggctgaagctcaaactcgtctacaaatggctacgggaaccttaactgctcatgccacccattgagctcACCGtcacgaaaattgattcttaataatcagaaaagacgaataaaaaagtagtatcaaatcttgtgaaatataattgcttatcaaatacttagataTliIXhoIHaeIIPspXIPaeR7ItENO2VP165,6205,6405,6605,6805,7005,7205,7405,760
    ttaggtttaaaaattgatacagttttataagttactttttcaaagactcgtgctgtctattgcataatgcactggaaggggaaaaaaaaggtgcacacgcgtggctttttcttgaatttgcagtttgaaaaatGCTGgatgtgagacgataaatcgcagccaagtgagtgaatagaatccaaatttttaactatgtcaaaatattcaatgaaaaagtttctgagcacgacagataacgtattacgtgaccttccccttttttttccacgtgtgcgcaccgaaaaagaacttaaacgtcaaactttttaCGACctacactctgctatttagcgtcggttcactcacttatcAleIMluItENO2ConL2ConR25,7805,8005,8205,8405,8605,8805,9005,9205,940
    atgacgcaccacggtcagacacggccacatcgtatctcacaggagcaagcgcgataggagcactcacacatagtacggtgatccgctgactcctttgcccaaataagacgtgagccactagtgcactgcagTACAcggtttccttgaaatttttttgattcggtaatctccgaactactgcgtggtgccagtctgtgccggtgtagcatagagtgtcctcgttcgcgctatcctcgtgagtgtgtatcatgccactaggcgactgaggaaacgggtttattctgcactcggtgatcacgtgacgtcATGTgccaaaggaactttaaaaaaactaagccattagaggcttgPstISpeIBmgBIConL2ConR2URA3 promoter5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agaaggaagaacgaaggaaggagcacagacttagattggtatatatacgcatatgtagtgttgaagaaacatgaaattgcccagtattcttaacccaactgcacagaacaaaaacgtgcaggaaacgaagataaatcatgtcgaaagctacatataaggaacgtgctgctactcatcttccttcttgcttccttcctcgtgtctgaatctaaccatatatatgcgtatacatcacaacttctttgtactttaacgggtcataagaattgggttgacgtgtcttgtttttgcacgtcctttgcttctatttagtacagctttcgatgtatattccttgcacgacgatgagtNdeIURA3 promoterURA3URA36,1406,1606,1806,2006,2206,2406,2606,2806,300
    tcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggagaggatcaggacaacgacggttcgataaattatagtacgtgcttttcgtttgtttgaacacacgaagtaacctacaagcatggtggttccttaatgacctcaatcaacttcgtaatccagggttttaaacaaatgatttttgtgtacacctatagaactgactaaaaaggtacctcNcoIXcmIEcoRVPpuMIEcoO109IURA3URA36,3206,3406,3606,3806,4006,4206,4406,460
    ggcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaggacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgccgtgtcaattcggcgatttccgtaataggcggttcatgttaaaaaatgagaagctcctgtcttttaaacgactgtaaccattatgtcagtttaacgtcatgagacgcccacatatgtcttatcgtcttacccgtctgtaatgcttacgtgtgccacaccacccgggtccataacAccIApaIScaIPspOMIBstZ17IURA3URA36,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttagcggtttgaagcaggcggcagaagaagtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctccctatctactggagaatatactaagggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagacataatcgccaaacttcgtccgccgtcttcttcattgtttccttggatctccggaaaactacaatcgtcttaacagtacgttcccgagggatagatgacctcttatatgattcccatgacaactgtaacgcttctcgctgtttctaaaacaatagccgaaataacgagtttctctgtaSapIStuIBspQIURA3URA36,6606,6806,7006,7206,7406,7606,7806,8006,820
    gggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagatgcattgggtcaacagtatagaaccgtggatgatgtggtttctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggtacccaccttctctacttccaatgctaaccaactaatactgtgggccacacccaaatctactgttccctctacgtaacccagttgtcatatcttggcacctactacaccaaagatgtcctagactgtaataataacaaccttctcctgataaacgtttcccttccctacgattccatBpu10IURA3URA36,8406,8606,8806,9006,9206,9406,9606,9807,000
    gagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaaaaaactgtattataagtaaatgcatgtatactaaactcacaaattagagcttcaatttaattactcccacttgcaatgtcttttcgtccgacccttcgtataaactcttctacgccggtcgttttgattttttgacataatattcatttacgtacatatgatttgagtgtttaatctcgaagttaaattaatAccIBseYIBstZ17IURA3URA3sgRNA-URA3URA3 terminator7,0207,0407,0607,0807,1007,120
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    7129 bp500055006000650050010001500ColE1ColE1 originAmpRNotIo53 Reverse Primer (DNA)

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    7129