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pKL1_354
  • Sequence Map
    Editing disabled on read-only sequence.
    AACGccttgccaacagggagttcttcagagacatggaggctcaaaacgaaattattgacagcctagacatcaatagtcatacaacagaaagcgaccacccaactttggctgataatagcgtataaacaatgcatactttgtacgttcaaaatacaatgcagtagatatatttatgTTGCggaacggttgtccctcaagaagtctctgtacctccgagttttgctttaataactgtcggatctgtagttatcagtatgttgtctttcgctggtgggttgaaaccgactattatcgcatatttgttacgtatgaaacatgcaagttttatgttacgtcatctatataaatacAjuINsiIpTEF1 (Promoter)pTEF120406080100120140160
    catattacatataatacatatcacataggaagcaacaggcgcgttggacttttaattttcgaggaccgcgaatccttacatcacacccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcggtataatgtatattatgtatagtgtatccttcgttgtccgcgcaacctgaaaattaaaagctcctggcgcttaggaatgtagtgtgggttagggggtgttcactagggggtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtctaaaagagcctgaggcgcNsiIpTEF1 (Promoter)pTEF1180200220240260280300320340
    catcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagacaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttctgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctgtggcggagcaaagaaaaagaagcagctttttccgttatttttaaaaatagtgcaaagaaaaagapTEF1 (Promoter)pTEF1360380400420440460480500520
    tgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcatcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcaattatttgccagtagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttagattagattcaaaattaatpTEF1 (Promoter)pTEF1540560580600620640660680700
    caaaagatcTATGgtgtccaaaggagaggagttaatcaaggaaaacatgagaatgaaagttgtcatggagggctccgttaatggtcaccaattcaagtgtacaggggaaggtgaaggtaatccttacatgggtacacaaactatgagaattaaagtaattgaaggcggaccactagttttctagATACcacaggtttcctctcctcaattagttccttttgtactcttactttcaacagtacctcccgaggcaattaccagtggttaagttcacatgtccccttccacttccattaggaatgtacccatgtgtttgatactcttaatttcattaacttccgcctggtgatBglIIBseRIBstEIImRuby2 (Gene Comp.)mRuby2pTEF...er)720740760780800820840860
    ccatttgcatttgacattctggcaacgtcattcatgtacggatcacgaactttcatcaagtaccctaaaggtataccagactttttcaagcaatcttttccagagggttttacatgggaaagggttacaagatacgaagatgggggtgtcgtcacagttatgcaagatacttcatggtaaacgtaaactgtaagaccgttgcagtaagtacatgcctagtgcttgaaagtagttcatgggatttccatatggtctgaaaaagttcgttagaaaaggtctcccaaaatgtaccctttcccaatgttctatgcttctacccccacagcagtgtcaatacgttctatgaagtaAccIBstZ17ImRuby2 (Gene Comp.)mRuby28809009209409609801,0001,0201,040
    tagaagatggctgccttgtctatcatgtgcaagtaagaggggtgaattttccttctaacggacctgtgatgcagaaaaagaccaaaggttgggaaccaaatactgaaatgatgtacccagctgatggaggtttgagaggctacacacacatggcgcttaaagttgatggtggaggatcttctaccgacggaacagatagtacacgttcattctccccacttaaaaggaagattgcctggacactacgtctttttctggtttccaacccttggtttatgactttactacatgggtcgactacctccaaactctccgatgtgtgtgtaccgcgaatttcaactaccacctccPflMIPflMIPvuIImRuby2 (Gene Comp.)mRuby21,0601,0801,1001,1201,1401,1601,1801,2001,220
    tcatttgtcttgtagttttgttaccacttatcgttctaaaaagactgttggcaatatcaaaatgccaggaatacatgctgtagaccacagactagaaagactcgaagagagcgataacgaaatgttcgttgtacagagagagcatgccgtagccaaatttgctggcttaggcggtagtaaacagaacatcaaaacaatggtgaatagcaagatttttctgacaaccgttatagttttacggtccttatgtacgacatctggtgtctgatctttctgagcttctctcgctattgctttacaagcaacatgtctctctcgtacggcatcggtttaaacgaccgaatccgccaAccISphImRuby2 (Gene Comp.)mRuby21,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggtatggatgaattgtataagggATCCtaactcgagATCCgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcaccatacctacttaacatattcccTAGGattgagctcTAGGcgagattggcttttccttcctcaatctgttggacttcagatccagggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttaaaaagaaaaaaaagacatgtctgcgcacatgcgtAvaIBplIBplIMluITliIXhoIBamHIBsoBIPpuMIPaeR7IEcoO109ICyc1 TerminatorCyc1 (Terminator)mRuby2 (Gene Comp.)mRuby21,4201,4401,4601,4801,5001,5201,5401,560
    tgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagGCTGagcatgagacggaaatctgctcgtcagtggtgctcacactgacgaatcatgtacagatcataccgatgactgcctggcgactcacaactaagcaagacagccggaaccagcgccggcgaaacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttcCGACtcgtactctgcctttagacgagcagtcaccacgagtgtgactgcttagtacatgtctagtatggctactgacggaccgctgagtgttgattcgttctgtcggccttggtcgcggccgcttBlpIMreINaeIBsmBIEsp3ISgrAINgoMIV3' Assembly ConnectorConREConRE'Cyc1 (Terminator)Cyc1 Terminator1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caccactgcatatatggcatatcacaacagtccaactagtgcactgcagTACAgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagtggtgacgtatataccgtatagtgttgtcaggttgatcacgtgacgtcATGTcaaatcgaacggagcaggggcggcccagtgggccggtcgctgtacctccgggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatPstISpeIXcmIBmgBIBstXIBstEIIHygromycinR (Yeast Marker)AgTEF promoter3' Assembly ConnectorConREConRE'1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctccagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatcgggtatgtaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcgtttttaatgccgaggagcgaggtctggacgctcgtccctttgcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaMluIBfuAIBseRIBspMIHygromycinR (Yeast Marker)AgTEF promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaaaatgggtaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtgtccgacctgatgcagctctcaacggtgactccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgtagtgtaggcttgtatttgtttttacccatttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcacaggctggactacgtcgagagAhdIZraIAatIIPshAIHygromycinR (Yeast Marker)HygromycinRAgTEF promoter2,1202,1402,1602,1802,2002,2202,2402,260
    ggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaatttagcgagagcctgcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaaatcgctctcggacHygromycinR (Yeast Marker)HygromycinR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    acctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcaaccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactatggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgttggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatAgeINcoIPvuIStyIAsiSIBfuAIBspMIRsrIIBstAPIDraIIIHygromycinR (Yeast Marker)HygromycinR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    catggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaBanINdeIPflFIBstAPIDraIIINmeAIIITth111IHygromycinR (Yeast Marker)HygromycinR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctcggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgagAjuISacIIHygromycinR (Yeast Marker)HygromycinR2,8202,8402,8602,8802,9002,9202,9402,960
    cgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgaHincIIHygromycinR (Yeast Marker)HygromycinR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataaagtaactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttcacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatttcattgactgttatttttctaagaacaaaagttcttgaacagtaaacatatcaaaaaaatataacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaHygromycinR (Yeast Marker)AgTEF terminatorHygromycinR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgGAGTtatcgtgttgcatctgcggctttaaattgatgtatctcatcgcaggcacgggcagtacagtgccctgagcgtagggaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatagtacgcagttagcatacacttacgaccagcgatatgacCTCAatagcacaacgtagacgccgaaatttaactacatagagtagcgtccgtgcccgtcatgtcacgggactcgcatccctttBsmIBsaXIHygromycinR (Yeast Marker)AgTEF terminatorHO 3' Homology (Yeast Origin)HO - 3' Homology3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aatgaaaaaaaggatgtaacttttaacataattccagcacgcagcgattgggtataatgaagattgttaagttcaacaacatgtgaaattcttagctccgcaacttgcttgaattatgagctctaagattcaagaagtaaataggtcatataatggaatgatagtagtacttgatttactttttttcctacattgaaaattgtattaaggtcgtgcgtcgctaacccatattacttctaacaattcaagttgttgtacactttaagaatcgaggcgttgaacgaacttaatactcgagattctaagttcttcatttatccagtatattaccttactatcatcatgaactaPciISacIEco53kIHO 3' Homology (Yeast Origin)HO - 3' Homology3,5203,5403,5603,5803,6003,6203,6403,660
    aaaggaaccatgtgatcttacgttgatatgaaaataattctctcacagaagtcagtttgtatgactaacatagagagtaattgcttttcggataaaatcgcacctaacaaagattaccttttgtaaagcctccagaacagctatgaatgttcgttattctacgcactttctttcatttccttggtacactagaatgcaactatacttttattaagagagtgtcttcagtcaaacatactgattgtatctctcattaacgaaaagcctattttagcgtggattgtttctaatggaaaacatttcggaggtcttgtcgatacttacaagcaataagatgcgtgaaagaaagtHO 3' Homology (Yeast Origin)HO - 3' Homology3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tacatcttgatccgccttttttgatgtaggttttaacgcggcaaaacatattaatttttctgatttggaaaaataaagagttagttctgccCCGAgcggccgcgattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgtagaactaggcggaaaaaactacatccaaaattgcgccgttttgtataattaaaaagactaaacctttttatttctcaatcaagacggGGCTcgccggcgctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatataAseIAvaINotIBsoBIHO 3' Homology (Yeast Origin)HO - 3' HomologyAmpR-ColE1 (Bac. Marker/Origin)AmpR terminator3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgggacccacgctctactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgccctgggtgcgagAhdIBanIBmrIKflIBsrDIPpuMISacIIEcoO109IAmpR-ColE1 (Bac. Marker/Origin)AmpRAmpR terminator4,0404,0604,0804,1004,1204,1404,1604,1804,200
    accggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtgtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcacAseIBglIBsrDINmeAIIIAmpR-ColE1 (Bac. Marker/Origin)AmpR4,2204,2404,2604,2804,3004,3204,3404,360
    gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcatacacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtatPvuIAmpR-ColE1 (Bac. Marker/Origin)AmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    attctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttctaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagBcgIXmnIAmpR-ColE1 (Bac. Marker/Origin)AmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaactAmpR-ColE1 (Bac. Marker/Origin)AmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgtcatgaccaaaatcccttaacgtgagttttcgttcctatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacagtactggttttagggaattgcactcaaaagcaaggSspIBspHIBspHIAmpR-ColE1 (Bac. Marker/Origin)AmpR promoterColE1 originColE14,9204,9404,9604,9805,0005,0205,0405,060
    actgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagattgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctaAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE15,0805,1005,1205,1405,1605,1805,2005,2205,240
    accaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccAlwNIAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE15,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttcagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaagAmpR-ColE1 (Bac. Marker/Origin)ColE1 originColE15,4405,4605,4805,5005,5205,5405,5605,5805,600
    cagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgPciIAmpR-ColE1 (Bac. Marker/Origin)ColE1ColE1 origin5,6205,6405,6605,6805,7005,7205,7405,760
    gttatcccctgattctgtggataaccgtgcggccgcCAATtctaattgtatcgagatcacttttcgtgatccgctaatcagcgacggtcacattaggtttgccaagtcagggtatgaaccatacgatcagttttcgtgaacctggtacgtatattgtggcgtttgtgtatattttcaataggggactaagacacctattggcacgccggcgGTTAagattaacatagctctagtgaaaagcactaggcgattagtcgctgccagtgtaatccaaacggttcagtcccatacttggtatgctagtcaaaagcacttggaccatgcatataacaccgcaaacacatataaaaNotIBsaAISexAISnaBIHO 5' Homology (Yeast Origin)HO - 5' HomologyAmpR-ColE1 (B...rker/Origin)ColE15,7805,8005,8205,8405,8605,8805,9005,9205,940
    cattctttgacaacaatcaataccaacctcaaataggaaaagtaataagtttggcgttacaccccaaaagacgccaaacggatcgaacttactcaatagcaattagcgagacaaaacctacgttaagacctgtaaccgatttatcaaagcactctgcggttctttcttgggaatagtaagaaactgttgttagttatggttggagtttatccttttcattattcaaaccgcaatgtggggttttctgcggtttgcctagcttgaatgagttatcgttaatcgctctgttttggatgcaattctggacattggctaaatagtttcgtgagacgccaagaaagaacccttatSspIEcoNIHO 5' Homology (Yeast Origin)HO - 5' Homology5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttacctggacattttgtgccctcaagaaacgaggctctacgagcctgttggagcccctcagacattagccgccacgaatcaaactttttacgcgattcggcccaaatcagtttctcacagatcattcgtagagtgaaaaagcacatcgattatttgatacccctttgggttaattaatggacctgtaaaacacgggagttctttgctccgagatgctcggacaacctcggggagtctgtaatcggcggtgcttagtttgaaaaatgcgctaagccgggtttagtcaaagagtgtctagtaagcatctcactttttcgtgtagctaataaactatggggaaacccaattaaClaIBspDIHO 5' Homology (Yeast Origin)HO - 5' HomologysgR...-26,1406,1606,1806,2006,2206,2406,2606,2806,300
    actgttgaggtctttgccgtaagttccatcgctgtCCCTgaattcgcatctagaacggggtcatcacggctcatcatgcgccaaacaaatgtgtgcaatacacgctcggatgactgcatgatgaccgcactgactggggacagcagatccacctaagcctgtgagagaagcagactgacaactccagaaacggcattcaaggtagcgacaGGGActtaagcgtagatcttgccccagtagtgccgagtagtacgcggtttgtttacacacgttatgtgcgagcctactgacgtactactggcgtgactgacccctgtcgtctaggtggattcggacactctcttcgtctgBmrIXbaIEcoRIConL1ConR1HO 5' Homology (Yeast Origin)HO - 5' Homology6,3206,3406,3606,3806,4006,4206,4406,460
    acccgacagatcaaggcagttacgtctcaccaaaccagatgtcaacacagctactgggctgtctagttccgtcaatgcagagtggtttggtctacagttgtgtcgatgBsmBIEsp3IHincIIConL1ConR1ConL1 Scar6,4806,5006,520
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    6529 bp450050005500600050010001500mRuby2

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    6529