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pJ401-8XHis-Tfu0868
  • Sequence Map
    Editing disabled on read-only sequence.
    taaggtctcaccccaagggcgacaccccctaattagcccgggcgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagtccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtattccagagtggggttcccgctgtgggggattaatcgggcccgctttccgggtcagaaagctgactcggaaagcaaaataaactacggaccgtcaagggatgagagcgtacccctcaggggtgtgatggtagccgcgatgccgcaaagtgaagactcaagccgtaccccagtccaSrfIStyIrrnB1 B2 T1 txn terminatorpTR20406080100120140160
    gggaccaccgcgctactgccgccaggcaaacaaggggtgttatgagccatattcaggtataaatgggctcgcgataatgttcagaattggttaattggttgtaacactgacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgaccctggtggcgcgatgacggcggtccgtttgttccccacaatactcggtataagtccatatttacccgagcgctattacaagtcttaaccaattaaccaacattgtgactggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactNruIBspHIrrnB1 B2 T1 ... terminatorAmpR promoter180200220240260280300320340
    taaatgcttcaataatattgaaaaaggaagaatatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgcatttacgaagttattataactttttccttcttatactcggtataagttgccctttgcagctccggcgctaatttaaggttgtacctacgactaaatatacccatatttacccgagcgctattacagcccgttagtccacgctgttagatagcgaacatacccttcgggctacgcgNruIKanR360380400420440460480500520
    cagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgccacttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcgttccagtctcaacaaagactttgtaccgtttccatcgcaacggttactacaatgtctactctaccagtctgatttgaccgactgccttaaatacggtgaaggctggtagttcgtaaaataggcatgaggactactacgtaccaatgagtggtgacgctaggggcctttttgtcgcaaggtKanR540560580600620640660680700
    ggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcactcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgcctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttccataatcttcttataggactaagtccacttttataacaactacgcgaccgtcacaaggacgcggccaacgtgagctaaggacaaacattaacaggaaaattgtcgctagcgcataaagcggagcgagtccgcgttagtgcttacttattgccaaaccaactacgctcactaaaaAjuIPvuIAsiSIEcoNIEcoNIKanR720740760780800820840860
    gatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccctactgctcgcattaccgaccggacaacttgttcagacctttctttacgtatttgaaaacggtaagagtggcctaagtcagcagtgagtaccactaaagagtgaactattggaataaaaactgctcccctttaattatccaacataactacaacctgctcagccttagcgtctggAseIKanR8809009209409609801,0001,0201,040
    gataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaagcggcgcgccatcgaatggcgcaaaacctttcctatggtcctagaacggtaggataccttgacggagccactcaaaagaggaagtaatgtctttgccgaaaaagtttttataccataactattaggactatacttatttaacgtcaaagtaaactacgagctactcaaaaagattcgccgcgcggtagcttaccgcgttttggaaagAscIPflMIPflMIKanR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatcgccataccgtactatcgcgggccttctctcagttaagtcccaccacttatactttggtcattgcaatatgctacagcgtctcatacggccacagagaatagtctggcaaagggcgcaccacttggtccggtcggtgcaaagacgcttttgcgccctttttcaccttcgccgctaAclIEarIBstAPILacI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaaccgcctcgacttaatgtaagggttggcgcaccgtgttgttgaccgcccgtttgtcagcaacgactaaccgcaacggtggaggtcagaccgggacgtgcgcggcagcgtttaacagcgccgctaatttagagcgcggctagttgacccacggtcgcaccaccacagctaccatcttLacI1,4201,4401,4601,4801,5001,5201,5401,560
    cgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtagcttcgccgcagcttcggacatttcgccgccacgtgttagaagagcgcgttgcgcagtcacccgactagtaattgataggcgacctactggtcctacggtaacgacaccttcgacggacgtgattacaaggccgcaataaagaactacagagactggtctgtgggtagttgtcatBclIMluIApaLIBsrDILacI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttattttctcccatgaggacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaataaaagagggtactcctgccatgcgctgacccgcacctcgtagaccagcgtaacccagtggtcgtttagcgcgacaatcgcccgggtaattcaagacagagccgcgcagacgcagaccgaccgaccgtatttatagagtgagcgttagtttaagtcggctatcgccttgccctBstEIILacI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    aggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgattccgctgacctcacggtacaggccaaaagttgtttggtacgtttacgacttactcccgtagcaagggtgacgctacgaccaacggttgctagtctaccgcgacccgcgttacgcgcggtaatggctcaggcccgacgcgcaaccacgcctatagagccatcaccctatgctgctaBsrDIEcoRVLacI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    accgaagatagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgccagtctcactggtgaaaagaaaaaccaccctggcgcccaatatggcttctatcgagtacaatatagggcggcaattggtggtagtttgtcctaaaagcggacgaccccgtttggtcgcacctggcgaacgacgttgagagagtcccggtccgccacttcccgttagtcgacaacggtcagagtgaccacttttctttttggtgggaccgcgggttatHpaIBsaXIPvuIILacI2,1202,1402,1602,1802,2002,2202,2402,260
    cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgactcatgaccaaaatcccttaacgtgagttacgcgcgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactgagtactggttttagggaattgcactcaatgcgcgcgcagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaAseIBspHIMauBIPvuIIpUC_oriLacI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttagcccaccacttcaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatcgggtggtgaagtAcuIpUC_ori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaactcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgSfcIAlwNIApaLIpUC_ori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtgSfcIBssSIBssSαIpUC_ori2,8202,8402,8602,8802,9002,9202,9402,960
    ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgaggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcNspIPciIpUC_oriVR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaggcgagagtagggaactgccaggcatcaaactaagcagaaggcccctgacggatggcctttttgcgtttctacaaactctttctgtgttgtaaaacgacggccagtcttaagcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttccgctctcatcccttgacggtccgtagtttgattcgtcttccggggactgcctaccggaaaaacgcaaagatgtttgagaaagacacaacattttgctgccggtcagaattcgAhdIAflIIM13 f...-20)pUC_ori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tcgggccccctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaagggcgcctgtcactttgcttgatatatgagaattatttaaccttataaatgagagcccgggggacccgccaagactattgctcattagcaattaggcgtttattgcatttttgggcgaagccgcccaaaaaaatacccccctcaaatccctttctcgtaaacagtcttataaattcccgcggacagtgaaacgaactatatactcttaataaattggaatatttactcPasIPsiIrpn txn terminatorbla txn terminator3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aaaaaagcaacgcactttaaataagatacgttgctttttcgattgatgaacacctataattaaactattcatctattatttatgattttttgtatatacaatatttctagtttgttaaagagaattaagaaaataaatctcgaaaataataaagggaaaatcagtttttgatatcttttttcgttgcgtgaaatttattctatgcaacgaaaaagctaactacttgtggatattaatttgataagtagataataaatactaaaaaacatatatgttataaagatcaaacaatttctcttaattcttttatttagagcttttattatttcccttttagtcaaaaactatagDraIEcoRVbla txn terminatorpTF33,5203,5403,5603,5803,6003,6203,6403,660
    aaaattatacatgtcaacgataatacaaaatataatacaaactataagatgttatcagtatttattatgcatttagaataaattttgtgtcgcccttTACACGTACTTAGTCGCTGAAaattgtgagcggataacaattacgagcttcatgcacagtTaaatcatAaaaaatttattttaatatgtacagttgctattatgttttatattatgtttgatattctacaatagtcataaataatacgtaaatcttatttaaaacacagcgggaaATGTGCATGAATCAGCGACTTttaacactcgcctattgttaatgctcgaagtacgtgtcaAtttagtaTtttttaaatNspIPciIBsaAIbla txn terminatorInsertionlacO3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacggtttccctctagaaataattttgtttaacttttcgagaccttaggaggtaaaacatatgcatcatcaccatcaccaccatcatGCGGGTCGACACCGGATTCTTCCAGCACaaacgaaacactcgcctattgttaatattatacaccttaacactcgcgagtgttaaggtgttgccaaagggagatctttattaaaacaaattgaaaagctctggaatcctccattttgtatacgtagtagtggtagtggtggtagtaCGCCCAGCTGTGGCCTAAGAAGGTCGTGAccIAfeINdeIPsiISalIXbaIBsu36IT5 promoterlacOi8XHis TagTfu_08683,8603,8803,9003,9203,9403,9603,9804,0004,020
    GGCGCCGTACCTTTGCACCCACCTGGGTCGTGCTGCTGGTCGCGGCCGGCGTGGTGGCCCTGTGCGGCGGGGTCGTCGTGCTCCTCGGCCCGCGCCTGGGGAACTGGTGGACCAGGCCCACGGCGGAGCGGATCGCCTACTTCGCTGACTGGAATACCGCCAACCGCGGCTACACCCGCGGCATGGAAACGTGGGTGGACCCAGCACGACGACCAGCGCCGGCCGCACCACCGGGACACGCCGCCCCAGCAGCACGAGGAGCCGGGCGCGGACCCCTTGACCACCTGGTCCGGGTGCCGCCTCGCCTAGCGGATGAAGCGACTGACCTTATGGCGGTTGGCGCCGATGTGEagINaeIBseRISacIINgoMIVTfu_08684,0404,0604,0804,1004,1204,1404,1604,1804,200
    GATCAAAGACGTGGACGAGAGCGGGGCTGCCGCACTCCTGGACCGGCTGATCTGGGCGTTCGGGGATGTGAGCGAAGACGGGCTGTGCCACATCCCCGCTGAGGCGGACCAGCCGTGGCAGATCTTCCAGCGCCGCTACGAGGCCGCGGAAAGCGTGGACGGCCAAGCCGACACCCTAGTTTCTGCACCTGCTCTCGCCCCGACGGCGTGAGGACCTGGCCGACTAGACCCGCAAGCCCCTACACTCGCTTCTGCCCGACACGGTGTAGGGGCGACTCCGCCTGGTCGGCACCGTCTAGAAGGTCGCGGCGATGCTCCGGCGCCTTTCGCACCTGCCGGTTCGGCTGTGGBbsIPfoIBbvCIBglIIBmgBISacIITfu_08684,2204,2404,2604,2804,3004,3204,3404,360
    TACGAGCAGCCGCTCGCCGGGAGCCTCAACCAGTTGCGCAAACTCCAGCAGAAGTACCCGGACTTGCGCGCCAGCATCTCGCTGGGGGGATGGAACTGGTCCACGTACTTTTCCGAGGCGGTGCGGACTCAGGAGTCCCGGGAGCGTTTCGTCGCCTCCTGCATCGACCTGTGGCATGCTCGTCGGCGAGCGGCCCTCGGAGTTGGTCAACGCGTTTGAGGTCGTCTTCATGGGCCTGAACGCGCGGTCGTAGAGCGACCCCCCTACCTTGACCAGGTGCATGAAAAGGCTCCGCCACGCCTGAGTCCTCAGGGCCCTCGCAAAGCAGCGGAGGACGTAGCTGGACACCGFspIBsaAITfu_08684,3804,4004,4204,4404,4604,4804,5004,5204,540
    TGCGCGGCAACCTGCCCCGCCTCGAGGGGGAGCCGCAGGGAGGCGAAGGGGTCGCCGCAGGGATTTTCGACGGTATCGACCTCGACTGGGAGTGGCCGGGCGGCAACGGCCATCCCGACAACGTCGAGCACCCCGACGACAAGCGGAATTTCACCCTCGTCGTGCAGGAGTTCCGACGCGCCGTTGGACGGGGCGGAGCTCCCCCTCGGCGTCCCTCCGCTTCCCCAGCGGCGTCCCTAAAAGCTGCCATAGCTGGAGCTGACCCTCACCGGCCCGCCGTTGCCGGTAGGGCTGTTGCAGCTCGTGGGGCTGCTGTTCGCCTTAAAGTGGGAGCAGCACGTCCTCAAGGCTliIXhoIBfuAIBspMIPflFIPspXIPaeR7ITth111ITfu_08684,5604,5804,6004,6204,6404,6604,6804,7004,720
    CGCCCAGTTGGACGCCTTGGAACGCGAGACCGGGCGGGAGTACACCCTGTCGGCGTCCCTGAGCCACGACCCGAAGATCATGCGGGACAGCTACGAGCCTGAGGTCTTCGACTACCTGGACTTCGCTACCGTCCAGGGCTACGACTTCACCGGGTCGTGGCGGGACATCACCGGCGCGGGTCAACCTGCGGAACCTTGCGCTCTGGCCCGCCCTCATGTGGGACAGCCGCAGGGACTCGGTGCTGGGCTTCTAGTACGCCCTGTCGATGCTCGGACTCCAGAAGCTGATGGACCTGAAGCGATGGCAGGTCCCGATGCTGAAGTGGCCCAGCACCGCCCTGTAGTGGCCGBbsIStyITatIBsu36ITfu_08684,7404,7604,7804,8004,8204,8404,8604,8804,900
    CACCACGCGCAGTTGTACGCGCCTGACCAGGATCCCGACCAGCCCAGCGTCGACCGGGCAGTCCGCCAGTACCTCGACTACGGGCTGCCCGCCGAGAAGCTCGTGCTCGGCATCCCCGGCTACGGTCGCGGCTGGCGCGGGGTGCCGCCGGGGCCCAACGGCGACGGGTGGCTGGGTGGTGCGCGTCAACATGCGCGGACTGGTCCTAGGGCTGGTCGGGTCGCAGCTGGCCCGTCAGGCGGTCATGGAGCTGATGCCCGACGGGCGGCTCTTCGAGCACGAGCCGTAGGGGCCGATGCCAGCGCCGACCGCGCCCCACGGCGGCCCCGGGTTGCCGCTGCCCACCGACCAccISalIBamHIBssSIBssSαITfu_08684,9204,9404,9604,9805,0005,0205,0405,060
    TCGAAGCGGAACAGCCGGCGGAAGGCGGCTACGGGGAGGCGACACACGCCTACGAAACACTGGAGCAGCATCCCGGGGAGCGTTTCTTCGACCGCGAGACCGGGGCCGCGTGGATCTACGACGGCGACGAGTGGTGGTCCTACGACACCCCGGAAGTGGTGCGCCGCAAAGGCGCAGCTTCGCCTTGTCGGCCGCCTTCCGCCGATGCCCCTCCGCTGTGTGCGGATGCTTTGTGACCTCGTCGTAGGGCCCCTCGCAAAGAAGCTGGCGCTCTGGCCCCGGCGCACCTAGATGCTGCCGCTGCTCACCACCAGGATGCTGTGGGGCCTTCACCACGCGGCGTTTCCGCGNaeINgoMIVTfu_08685,0805,1005,1205,1405,1605,1805,2005,2205,240
    CTACGCCGTCCGCCACGGCCTGCGCGGCCTGGTGCTGTGGAACCTGGACATGGATCCGGAAGGGGAGCTGGTCGCCGCGATGGCGGAGTCGCTGCGCTGAGATGCGGCAGGCGGTGCCGGACGCGCCGGACCACGACACCTTGGACCTGTACCTAGGCCTTCCCCTCGACCAGCGGCGCTACCGCCTCAGCGACGCGACTSfiIBamHIBspEITfu_08685,2605,2805,3005,3205,340
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Assembly Parameters
    • Linear Map
    Editing disabled on read-only sequence.
    5350 bp4000425045004750500025050075010001250LacIpTRpJ401-mCherry FWD (DNA)Tfu_0868 PCR product FWD (DNA)Tfu_0868 PCR product REV (DNA)

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    5350