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Assuring the current protocol works, the following chips are fabricated to increase height to 4-5mm vs the previous achieved height of 2-3mm, based on 2 factors;
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Steady PDMS total volume
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Losses during pouring
PROCEDURE
Differences introduced (vs protocol)
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Aluminum foil was NOT used for the petri dishes, as a well cleaned, glass dish was sufficient for the procedure and decreased the losses of PDMS volume
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A thin, bendable silicone spatula was used to pour the PDMS into the wafer and minimized losses almost to 0%. Pictures depict the used spatula, which has been stored with the glass petri dishes used for fabrication. (Soon to be added in the Inventory doc.)
1724744324848.jpg
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1724744324831.jpg
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The following pictures show the technique that was used to cover the wafer with aluminium foil to reduce radius by ~1 cm and ensure no leakage.
20240826_143944.jpg
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20240826_143952.jpg
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Preparation
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Clean the glass petri dishes using ethanol or acetone
Fabrication
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Time in the desiccator: 45'
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Baking time: 30'
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Time in the desiccator: 30'
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Baking time: 30'
OUTCOMES
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Chips #29 - 36 (DM) came out in good condition with a height of 4mm (#29 - 30) and 4.5mm (#31 - 36)
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Chip #28 didn't make it through, it was ruptured during cutting.
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Chips #37 - 43 (EQM) came out in good condition with consistent chip height / wafer equaling to 4.5mm (#37 - 39) and 5mm (#40 - 43).
Cut sites of enzymes that you select are highlighted to help guide your work.
(Enzymes with compatible ends turn the same color.)
Selecting cut sites and copying the sequence will also activate enzymes.
See the "Cloning by restriction enzyme digest" tutorial under Sequence Construction in Help for more information.