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pRSF vector (plasmid backbone)
  • Sequence Map
    Editing disabled on read-only sequence.
    ggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccgaattcgagctcggcgcgcctgcaggtcgacaagcttgcggccgcataatgcttaagtcgaacaccccttaacactcgcctattgttaaggggacatctttattaaaacaaattgaaattattcctctatatggtacccgtcgtcggtagtggtagtagtggtgtcggtcctaggcttaagctcgagccgcgcggacgtccagctgttcgaacgccggcgtattacgaattcagcttgtAccIAscIBcgIBtgINcoINotIPstISacISalISbfIAflIIBamHIBfuAIBspMIEcoRIBssHIIEco53kIHindIIINmeAIIImultiple cloning site 1The lac r... (IPTG).6xHissource20406080100120140160
    gaaagtaatcgtattgtacacggccgcataatcgaaattaatacgactcactataggggaattgtgagcggataacaattccccatcttagtatattagttaagtataagaaggagatatacatatggcagatctcaattggatatcggccggccacgcgatcgctgacgtcggtctttcattagcataacatgtgccggcgtattagctttaattatgctgagtgatatccccttaacactcgcctattgttaaggggtagaatcatataatcaattcatattcttcctctatatgtataccgtctagagttaacctatagccggccggtgcgctagcgactgcagccaBaeIBarIFseIMfeINaeINdeIPvuITatIZraIAatIIAsiSIBglIIBsrGIEcoRVAcc65INgoMIVmultiple cloning site 2The lac r... (IPTG).promot...erasesource180200220240260280300320340
    accctcgagtctggtaaagaaaccgctgctgcgaaatttgaacgccagcacatggactcgtctactagcgcagcttaattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaacctcaggcattgggagctcagaccatttctttggcgacgacgctttaaacttgcggtcgtgtacctgagcagatgatcgcgtcgaattaattggatccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttggagtccgtaAccIAvaIBlpIKpnIPacITliIXhoIAvrIIBsoBIPspXIBsu36IPaeR7IEcoO109Imultiple cloning site 2transcription termina...ge T7 RNA polymeraseS-Tagsource360380400420440460480500520
    ttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgacaagctgacgaccgggtctccgcaagtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaactcttcgtgtgccagtgtgacgaaggccatcagttatttggccatttggtcgttatctgtattcgccgataaattgctgggacgggacttggctgctgttcgactgctggcccagaggcgttcaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagaAgeIBsaIDrdIBsaXIPflFITth111Isource540560580600620640660680700
    aaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatccttttatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaXmnIBspHIaph(3')-Ia CDSsource720740760780800820840860
    ggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagNsiIaph(3')-Ia CDSsource8809009209409609801,0001,0201,040
    gtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcggtcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgccagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagaAjuIBsmISspIBsmBIEcoNIEsp3IBpu10Iaph(3')-Ia CDSsource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgtAvaIBsmINsiISmaIXmaIBsoBITspMIaph(3')-Ia CDSsource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    actctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcatactcttcctttttcaatattatgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtatgagaaggaaaaagttataatClaINruISspIBspDIaph(3')-Ia CDSbla promotersource1,4201,4401,4601,4801,5001,5201,5401,560
    ttgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggcatgcagcgctcttccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggtgtcagctcactcaaaagcggtaatacggttaaacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccgtacgtcgcgagaaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgccacagtcgagtgagttttcgccattatgccaatAfeINspISapISphIBspHIBspQIPlasmids containing the RSF 1030 origin o...tional plasmids with compatible origins.bla promotersource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tccacagaatcaggggataaagccggaaagaacatgtgagcaaaaagcaaagcaccggaagaagccaacgccgcaggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagccagaggtggcgaaacccgacaggactataaagataccaggcgaggtgtcttagtcccctatttcggcctttcttgtacactcgtttttcgtttcgtggccttcttcggttgcggcgtccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcggtctccaccgctttgggctgtcctgatatttctatggtccgcNspIPciIAflIIIPlasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that containadditional plasmids with compatible origins.source1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgttggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacaaccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcApaLIBssSIBssSαIPlasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that containadditional plasmids with compatible origins.source1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccattggtaactgatttagaggactttgtcttgaagttatgcacctgttaaggctaaactgaaagaacagattttggtgagtgcggtcctcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtaaccattgactaaatctcctgaaacagaacttcaatacgtggacaattccgatttgactttcttgtctaaaaccactcacgccaggagAvaIIPflFITth111IPlasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that containadditional plasmids with compatible origins.source2,1202,1402,1602,1802,2002,2202,2402,260
    caacccacttaccttggttcaaagagttggtagctcagcgaaccttgagaaaaccaccgttggtagcggtggtttttctttatttatgagatgatgaatcaatcggtctatcaagtcaacgaacagctattccgttactctagatttcagtgcaatttatctcttcaaatgtagcgttgggtgaatggaaccaagtttctcaaccatcgagtcgcttggaactcttttggtggcaaccatcgccaccaaaaagaaataaatactctactacttagttagccagatagttcagttgcttgtcgataaggcaatgagatctaaagtcacgttaaatagagaagtttacatcgAloIBlpIXbaICspCIPlasmids containing the RSF 1030 origin of replication can be pro...i cells that containadditional plasmids with compatible origins.source2,2802,3002,3202,3402,3602,3802,4002,4202,440
    acctgaagtcagccccatacgatataagttgtaattctcatgttagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagttggacttcagtcggggtatgctatattcaacattaagagtacaatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcaAcuIlacI CDSsource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccgccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggKasINarISfoIAvaIIBsaXIBsmBIEsp3IPluTIPvuIIPvuIIlacI CDSsource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctacaggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccBbsIHpaIBsrDIBtgZIBssHIIlacI CDSsource2,8202,8402,8602,8802,9002,9202,9402,960
    aacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgcttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgApaIBcgIBpmIPspOMINmeAIIIlacI CDSsource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcaaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtBbsIBclIBsgIBsrDIBstEIIlacI CDSsource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttaBsgIMluIApaLIAflIIIlacI CDSsource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgAclIBpmIBtgZIBstAPIlacI CDSsource3,5203,5403,5603,5803,6003,6203,6403,660
    gttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaaattaatacgactcactatacaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatcctttaattatgctgagtgatatPfoIEcoNIlacI promoterlacI CDSsourcepromo...rase3,6803,7003,7203,7403,7603,7803,8003,820
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    3829 bp2750300032503500250500750lacI CDSS-Tagsource

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    3829