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ROS Assay Protocol
Thursday, 9/16/2021
Material
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MQ
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HRP
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Luminol or LO/12
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Elicitor
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Leaf disks
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96-well plate
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Falcon tubes (one per elicitor + 2)
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flg-22 (positive control)
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Plastik thingy for multipipette
Protocol
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Grow plants
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Take leaf disks
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Fill one row of the 96-well plate per elicitor with 100 μl MQ and add one leaf disk per well
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Cover in aluminium foil and let rest over night (in the dark)
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Cover all falcon tubes in aluminium foil before adding luminol or Lo/12
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Preparing the ROS solution:
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2ml MilliQ H2O per row of
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2miL HRP
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2miL luminol | LO/12 (needs to be kept dark)
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add everything to small falcon tubes
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decide on concentration of elicitor and add elicitor (one per falcon tube)
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Take out the 100 ml MQ of each well
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Go to the plate reader and add the ROS solution to the well plate there.