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6/6: Initial Wave Bioreactor Agitation Tests

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6/6: Initial Wave Bioreactor Agitation Tests

Sunday, 6/9/2019 Objectives Significant Notes Expected results Results Sunday, 6/16/2019 Growth Results after Agitation Tests image.png image.png image.png image.png image.png image.png image.png image.png

Sunday, 6/9/2019

Objectives

The objective of this K. Rhaeticus Agitation Test is to see what would happen to the cell culture if it were placed in a wave bioreactor (as opposed to using the sparging method or a static bioreactor).

Significant Notes

Everything went really smoothly, except when it came to pipetting the K.Rhaeticus cell culture into the 150 mm petri dishes because there was already a big clump of BC that formed which made pipetting a little challenging because the tip would catch onto a piece of the BC.

Expected results

The bacterial cellulose to grow in an even layer using the wave bioreactor.

Results

Sunday, 6/16/2019

Pictures:

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culture 2 sideview

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culture 2

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culture 1 sideview

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culture 2 sideview

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culture 1 sideview

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control

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culture 1 in the foreground, culture 2 in the background

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culture 2

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