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ABS44 (p2301Y-tOCS-pUBQ1:NLS-Venus-LucPlus-tUBQ1-pU6:pUBQ1_gRNA_Target1-tU6-pUBQ10:dCas9-NdC_IAA17-TPLRD2-tNos)
  • Sequence Map
    Editing disabled on read-only sequence.
    ACGCGTCCTGCAGGCGCGCCgaggggatcgagcccctgctgagcctcgacatgttgtcgcaaaattcgccctggacccgcccaacgatttgtcgtcactgtcaaggtttgacctgcacttcatttggggcccacatacaccaaaaaaatgctgcataattctcggggcagcaagtTGCGCAGGACGTCCGCGCGGctcccctagctcggggacgactcggagctgtacaacagcgttttaagcgggacctgggcgggttgctaaacagcagtgacagttccaaactggacgtgaagtaaaccccgggtgtatgtggtttttttacgacgtattaagagccccgtcgttcaApaIAscIBlpISbfIPspOMItOCSRareCut...cISbfI20406080100120140160
    cggttacccggccgccgtgctggaccgggttgaatggtgcccgtaactttcggtagagcggacggccaatactcaacttcaaggaatctcacccatgcgcgccggcggggaaccggagttcccttcagtgaacgttattagttcgccgctcggtgtgtcgtagatactagcccctgccaatgggccggcggcacgacctggcccaacttaccacgggcattgaaagccatctcgcctgccggttatgagttgaagttccttagagtgggtacgcgcggccgccccttggcctcaagggaagtcacttgcaataatcaagcggcgagccacacagcatctatgatcggggaMreItOCS180200220240260280300320340
    ggggccttttgaaatttgaataagatttatgtaatcagtcttttaggtttgaccggttctgccgctttttttaaaattggatttgtaataataaaacgcaattgtttgttattgtggcgctctatcatagatgtcgctataaacctattcagcacaatatattgttttcattttaccccggaaaactttaaacttattctaaatacattagtcagaaaatccaaactggccaagacggcgaaaaaaattttaacctaaacattattattttgcgttaacaaacaataacaccgcgagatagtatctacagcgatatttggataagtcgtgttatataacaaaagtaaaattOCS360380400420440460480500520
    atattgtacatataagtagtagggtacaatcagtaaattgaacggagaatattattcataaaaatacgatagtaacgggtgatatattcattagaatgaaccgaaaccggcggtaaggatctgagctacacatgctcaggttttttacaacgtgcacaacagaattgaaagcaaatataacatgtatattcatcatcccatgttagtcatttaacttgcctcttataataagtatttttatgctatcattgcccactatataagtaatcttacttggctttggccgccattcctagactcgatgtgtacgagtccaaaaaatgttgcacgtgttgtcttaactttcgttttOCS540560580600620640660680700
    tatcatgcgatcataggcgtctcgcatatctcattaaagcagCACGTGATTTTGTAATATCCCGGGATATTTCACAAATTGAACATAGACTACAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTTAGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAatagtacgctagtatccgcagagcgtatagagtaatttcgtcGTGCACTAAAACATTATAGGGCCCTATAAAGTGTTTAACTTGTATCTGATGTCTTAAAATCTTTTGTTTGAAAGAGAGAGAATAGAGTGGAAATAGAAAATCTCTCTTTTTCAAGCTAAAGGCCAACTGGCCTSmaIXmaITspMIpUBQ1tOCS720740760780800820840860
    ATGTATCTTTGTTTTTTTTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCCGTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTTAGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCCTCATCATCTACATAGAAACAAAAAAAACAAAACATTGTATAAAGCAAAAGGCTAAATCTAGCCTAGAGGAAAAGGCAAAACAGCCTGGAAGAAGGCCAAATAGGCCTAGATTATTATAGGTAGAATCTGAATCGATTCAAACCTAGACAAAAAACCAATCGAGAACAGTTAGCGGAGTAGTAGpUBQ18809009209409609801,0001,0201,040
    AGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCATGTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACTCTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCATATGAAACCTTCGACTAACGAGAAGTGGTCTTTTCGTTCTTCCACTTTAAAAACTGTTTATTTAGAATCTTAGTACATCACAGAAACCTGGAACCCTTACTATCTTTGCTAAACAATATCGATGAGATACATAGTCTGGGACTGGTTCTAGGTTGTTAGAGTATCCAAAACACGTATACTTTGGAAGCTGATTGCTCTTCACCAGAAApUBQ11,0601,0801,1001,1201,1401,1601,1801,2001,220
    TAATGAGAGAGATATCTAAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAATGCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTAGCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCGCCTCCTCCATTACTCTCTCTATAGATTTTACAATAGAATTTTCGGGTGAGTTTAGAGTTCCGTATTCCATCTTTACGTTTAAACCTTTCACCCGACCCGGAAAACACCATTTCCGGACATTGGATCGGGTTATAATCGTTTTGGGATCTGCGCATGTAACTGTATATATTTGGGCGGAGGAGGStuIpUBQ1pUBQ1_g...arget11,2401,2601,2801,3001,3201,3401,3601,3801,400
    TTGTTTAGGGTTTCTACGTGAGAGAAGACGAAACACAAAAGatgccaaagaagaagaggaaagtcggaagtggtagtggtagtggaagcggtagtggcagtgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaAACAAATCCCAAAGATGCACTCTCTTCTGCTTTGTGTTTTCtacggtttcttcttctcctttcagccttcaccatcaccatcaccttcgccatcaccgtcacactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtVENUS-N7pUBQ1GS12bSV40NLS1,4201,4401,4601,4801,5001,5201,5401,560
    caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcgactagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggacccgatgccggacgtcacgaagcgggcgatggggctggtgtacttcgtcgtgctgaagVENUS-N71,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgaccVENUS-N71,7601,7801,8001,8201,8401,8601,8801,9001,920
    agtacaactacaacagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactatcatgttgatgttgtcggtgttgcagatatagtggcggctgttcgtcttcttgccgtagttccggttgaagttctaggcggtgttgtagctcctgccgccgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatVENUS-N71,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacggcagtggctcaggatcaggatctggatctggctctatggtcaccgacgccaaaaacataaagggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgccgtcaccgagtcctagtcctagacctagaccgagataccagtggctgcggtttttgtatttcMV2TD4AK6NI8K2249-3901Y1VENUS-N7GS12cluc+ genepr2682,1202,1402,1602,1802,2002,2202,2402,260
    aaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatttccgggccgcggtaagataggcgaccttctaccttggcgacctctcgttgacgtattccgatacttctctatgcgggaccaaggaccttgttaacgaaaatgtctacgtgtatagctccacctgtagtgaatgcgactcatgaagctttacaggcaagccaaccgtcttcgatK10GP12AP14FY16PL18ED20GT22AG24EQ26LH28KA30MK32RY34AL36VP38GT40IA42FT44DA46HI48EV50DI52TY54AE56YF58EM60SV62RL64AE66A2249-3901luc+ gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtactttgctatacccgacttatgtttagtgtcttagcagcatacgtcacttttgagagaagttaagaaatacggccacaacccgcgcaataaatagcctcaacgtcaacgcgggcgcttgctgtaaatattacttgcacttaacgagttgtcatacccgtaaagcgtcggatggcaM68KR70YG72LN74TN76HR78IV80VC82SE84NS86LQ88FF90MP92VL94GA96LF98IG100VA102VA104PA106ND108IY110NE112RE114LL116NS118MG120IS122QP124TV1262249-3901luc+ gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccaccacaagcaaaggtttttccccaacgttttttaaaacttgcacgtttttttcgagggttagtaggttttttaataatagtacctaagattttgcctaatggtccctaaagtcagctacatgtgcaagcagtgtagagtagatggagggccaaaattacttatgctaaaacacggtVF128VS130KK132GL134QK136IL138NV140QK142KL144PI146IQ148KI150II152MD154SK156TD158YQ160GF162QS164MY166TF168VT170SH172LP174PG176FN178EY180DF182VP1842249-3901luc+ gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttcctcaggaagctatccctgttctgttaacgtgactagtacttgaggagacctagatgaccagacggatttccacagcgagacggagtatcttgacggacgcactctaagagcgtacggtctctaggataaaaaccgttagtttagtaaggcctatgacgctaaaattcacaacaagES186FD188RD190KT192IA194LI196MN198SS200GS202TG204LP206KG208VA210LP212HR214TA216CV218RF220SH222AR224DP226IF228GN230QI232IP234DT236AI238LS240VV2422249-3901luc+ gene2,8202,8402,8602,8802,9002,9202,9402,960
    cattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgtaaggtagtgccaaaaccttacaaatgatgtgagcctataaactatacacctaaagctcagcagaattacatatctaaacttcttctcgacaaagactcctcggaagtcctaatgttctaagtttcacgcgacgaccacggttgggataagaggaagaagcggttttcgtgagaPF244HH246GF248GM250FT252TL254GY256LI258CG260FR262VV264LM266YR268FE270EE272LF274LR276SL278QD280YK282IQ284SA286LL288VP290TL292FS294FF296AK298ST300L2249-3901luc+ gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaactaactgtttatgctaaatagattaaatgtgctttaacgaagaccaccgcgaggggagagattccttcagccccttcgccaacggttctccaaggtagacggtccatagtccgttcctatacccgagtgactctgatgtagtcgataagactaatgtgggctccccctactatttI302DK304YD306LS308NL310HE312IA314SG316GA318PL320SK322EV324GE326AV328AK330RF332HL334PG336IR338QG340YG342LT344ET346TS348AI350LI352TP354EG356DD358K2249-3901luc+ gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggcccgcgccagccatttcaacaaggtaaaaaacttcgcttccaacacctagacctatggcccttttgcgacccgcaattagtttctccgcttgacacacactctccaggatactaatacaggccaatacatttgttaggccttcgctggttgcggaactaactgttcctacctaP360GA362VG364KV366VP368FF370EA372KV374VD376LD378TG380KT382LG384VN386QR388GE390LC392VR394GP396MI398MS400GY402VN404NP406EA408TN410AL412ID414KD416G2249-3901luc+ gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgaccgatgtaagacctctgtatcgaatgaccctgcttctgcttgtgaagaagtagcaactggcggacttcagagactaattcatgtttccgatagtccaccgagggcgacttaaccttaggtagaacgaggttgtggggttgtagaagctgcgtccacagcgtccagaagggctgctPaqCIW418LH420SG422DI424AY426WD428ED430EH432FF434IV436DR438LK440SL442IK444YK446GY448QV450AP452AE454LE456SI458LL460QH462PN464IF466DA468GV470AG472LP474DD4762249-3901luc+ gene3,5203,5403,5603,5803,6003,6203,6403,660
    tgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaactgcggccacttgaagggcggcggcaacaacaaaacctcgtgcctttctgctactgcctttttctctagcacctaatgcagcggtcagttcattgttggcgctttttcaacgcgcctcctcaacacaaacacctgcttcatggctttccagaatggccttttgagctgcgttctDA478GE480LP482AA484VV486VL488EH490GK492TM494TE496KE498IV500DY502VA504SQ506VT508TA510KK512LR514GG516VV518FV520DE522VP524KG526LT528GK530LD532AR5342249-3901luc+ gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaaAGAGACTCTTATCAAGAATCCCATCTCTTGCTTGCttttttttGTTGTCTTCCCTTTGATAGGGTTTGtttttCTTGTTTCAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATttttagtctctctaggagtatttccggttcttcccgcctttctagcggcacattTCTCTGAGAATAGTTCTTAGGGTAGAGAACGAACGaaaaaaaaCAACAGAAGGGAAACTATCCCAAACaaaaaGAACAAAGTCACTGAAAGATACAATTTTCTATTACAGTCATTTTCCTAKI536RE538IL540IK542AK544KG546GK548IA550V2249-3901tUBQ1luc+ gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTGGTTTTCTATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTATGTGATTAGGAGACGTGTTTCATTAATAAAATATAAGATCAATACATTGTTAGTAGTGATAAACTATGTACAAATTGTATTGATTGTAAAAACCAAAAGATAATAAGACTTAGCTAATGCCTTCTAAGAACGAATTAAGGTTAGATATGTTCATAGCACTTTATTACTGGCAAATACACTAATCCTCTGCACAAAGTAATTATTTTATATTCTAGTTATGTAACAATCATCACTATTTGATACATGTTTAACATAACTAACATTTtUBQ14,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGAAACACAATAGGTTCCttttttCTACAATATATTGTGACAGACTCTCTGTTTTAACGAATGAATTAAATTTGTCAACGaaaaaaTTAAAGAATGATGTGATCACTTTGCACCTTGGTTCATCTTCAGAGGCTTCTGCATGTTCCATGCATTGAGGCTCTCTACGGTCACAGGTTCTTTGTGTTATCCAAGGaaaaaaGATGTTATATAACACTGTCTGAGAGACAAAATTGCTTACTTAATTTAAACAGTTGCttttttAATTTCTTACTACACTAGTGAAACGTGGAACCAAGTAGAAGTCTCCGAAGACGTACAAGGTACGTAACTCCGAGAGATGCCAGTGTCCAtUBQ14,2204,2404,2604,2804,3004,3204,3404,360
    TGTGAGCCGTTGTTAAAGACAAACAAATGTCGACGTAAAGCCTGTAGAAGAGGTTTCTAGCGAACGACACGAGTTTGAGCCTCATGAAGCTTCGTTGAACAACGGAAACTCGACTTGCCTTCCGCACAATACATCATTTCTTCTTAGCTTTTTTTCTTCTTCTTCGTTCATACAGACACTCGGCAACAATTTCTGTTTGTTTACAGCTGCATTTCGGACATCTTCTCCAAAGATCGCTTGCTGTGCTCAAACTCGGAGTACTTCGAAGCAACTTGTTGCCTTTGAGCTGAACGGAAGGCGTGTTATGTAGTAAAGAAGAATCGAAAAAAAGAAGAAGAAGCAAGTATGTCSalIHindIIIU6 promoter papertUBQ14,3804,4004,4204,4404,4604,4804,5004,5204,540
    TTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAAAAAAAACAAATAGTCGAATGTAAAAGAACTTGGCATCGAAAGCAAAAGAAGAAAAATTGAAAGGTAAGCCTCAAAAACATAGAACAAAGTATCAAACAGGGTCCTAATCTTACTAATCCGTAGCTTGGAAGTTCTTAAACTAACTTATTTTGTAGAAGTAAGAATTCTATACU6 promoter paper4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAATTCTATTAGAAGTTTTCCGGGGACCCTTAGACTTTCTTCTCTTCGTCCGGGTAAATATACCCTTTCTTGTTATCATAAAGAATATATCCGGGTAAATTCAACTTTTGTTAGAAGTTTTCAGGGTGTAGCGAATCTATTCTTTTGCTTCGACTCAAATATATGTCGATCTCAGCTTU6 promoter paper4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTAGTGATTAAAGTGGGCTGGGCCTTTTGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttTTTGCAAAATTTTCCAGATCGATTTCTTCTTCCTCTGTTCTTCGGCGTTCAATTTCTGGGTTTTTCCATCACTAATTTCACCCGACCCGGAAAACcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaAAACGTTTTAAAAGGTCTAGCTAAAGAAGAAGGAGACAAGAAGCCGCAAGTTAAAGACCCAAAAAGgRNA HandleU6 terminator paperpUBQ1_g...arget14,9204,9404,9604,9805,0005,0205,0405,060
    TCTTCGTTTTCTGTAACTGAAACCTAAAATTTGACCTAAAAAAAATCTCAAATAATATGATTCAGTGGTTTTGTACTTTTCAGTTAGTTGAGTTTTGCAGTTCCGATGAGATAAACCAATAACTTTGCTTAGATCTAATTCATTCCGTTACACCTCTGATGGAGATGGAAGGTTCAGAAGCAAAAGACATTGACTTTGGATTTTAAACTGGATTTTTTTTAGAGTTTATTATACTAAGTCACCAAAACATGAAAAGTCAATCAACTCAAAACGTCAAGGCTACTCTATTTGGTTATTGAAACGAATCTAGATTAAGTAAGGCAATGTGGAGACTACCTCTACCTTCCAAGU6 terminator paper5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTAATAATGATGCCATTTTTTGGGTAATAATTTTGAATTAGAATCAAGGGTATAAGATTCATAATTAACATCACTTAAGCAAAGTTCGTAATATACGACCACAGGATATAATTTTTGGTACCTCAACAACAAACTTTCCATTCACCCATTTATGGATACACAGTCTAGCTCAACAAATTATTACTACGGTAAAAAACCCATTATTAAAACTTAATCTTAGTTCCCATATTCTAAGTATTAATTGTAGTGAATTCGTTTCAAGCATTATATGCTGGTGTCCTATATTAAAAACCATGGAGTTGTTGTTTGAAAGGTAAGTGGGTAAATACCTATGTGTCAGATCGAGTTGTKpnIAcc65IU6 terminator paperUBQ10 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GAGCTTTTAACCCAAATTGGTACAATAGAATACAACTTTAGATCATAATTCTCAAAAGAAAGAGATTCCTTAGCTATTCTATCTGCCACTCCATTTCCTTCTCGGCTTGTATGCACAAGCATAAAATCCTCAAACTTGCTAAGTAGATACTTTATGTCTTGGATAATTGGATTGACTCGAAAATTGGGTTTAACCATGTTATCTTATGTTGAAATCTAGTATTAAGAGTTTTCTTTCTCTAAGGAATCGATAAGATAGACGGTGAGGTAAAGGAAGAGCCGAACATACGTGTTCGTATTTTAGGAGTTTGAACGATTCATCTATGAAATACAGAACCTATTAACCTAACTUBQ10 promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GACTTGACAAGCATAACTTTCATGTAACCAAAGACACAAGTTGCTGAGAATCCACCTCAAAAATGATCTTCCTATAATTGAATCGGGATAATGACAGCACAGCCCATCTAAGAGCCTCCACTTCTACTTCCAGCACGCTTCTTACTTTTACCACAGCTCTTGCACCTAACCATAACTGAACTGTTCGTATTGAAAGTACATTGGTTTCTGTGTTCAACGACTCTTAGGTGGAGTTTTTACTAGAAGGATATTAACTTAGCCCTATTACTGTCGTGTCGGGTAGATTCTCGGAGGTGAAGATGAAGGTCGTGCGAAGAATGAAAATGGTGTCGAGAACGTGGATTGGTATTUBQ10 promoter5,6205,6405,6605,6805,7005,7205,7405,760
    CACCTTCCCTGTATGATCGCGAAGCACCCACCCTAAGCCACATTTTAATCCTTCTGTTGGCCATGCCCCATCAAAGTTGCACTTAACCCAAGATTGTGGTGGAGCTTCCCATGTTTCTCGTCTGTCCCGACGGTGTTGTGGTTGGTGCTTTCCTTACATTCTGAGCCTCTTTCCTGTGGAAGGGACATACTAGCGCTTCGTGGGTGGGATTCGGTGTAAAATTAGGAAGACAACCGGTACGGGGTAGTTTCAACGTGAATTGGGTTCTAACACCACCTCGAAGGGTACAAAGAGCAGACAGGGCTGCCACAACACCAACCACGAAAGGAATGTAAGACTCGGAGAAAGGANruIUBQ10 promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TCTAATCCACTCATCTGCATCTTCTTGTGTCCTTACTAATACCTCATTGGTTCCAAATTCCCTCCCTTTAAGCACCAGCTCGTTTCTGTTCTTCCACAGCCTCCCAAGTATCCAAGGGACTAAAGCCTCCACATTCTTCAGATCAGGATATTCTTGTTTAAGATGTTGAACTCTAAGATTAGGTGAGTAGACGTAGAAGAACACAGGAATGATTATGGAGTAACCAAGGTTTAAGGGAGGGAAATTCGTGGTCGAGCAAAGACAAGAAGGTGTCGGAGGGTTCATAGGTTCCCTGATTTCGGAGGTGTAAGAAGTCTAGTCCTATAAGAACAAATTCTACAACTTGAGATUBQ10 promoterUBQ10f p...binding5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGGAGGTTTGTATGAACTGATGATCTAGGACCGGATAAGTTCCCTTCTTCATAGCGAACTTATTCAAAGAATGTTTTGTGTATCATTCTTGTTACATTGTTATTAATGAAAAAATATTATTGGTCATTGGACTGAACACGAGTGTTAAATATGGACCAGGCCCCAAATAAGATCCACCTCCAAACATACTTGACTACTAGATCCTGGCCTATTCAAGGGAAGAAGTATCGCTTGAATAAGTTTCTTACAAAACACATAGTAAGAACAATGTAACAATAATTACTTTTTTATAATAACCAGTAACCTGACTTGTGCTCACAATTTATACCTGGTCCGGGGTTTATTCTAGGUBQ10 promoter6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ATTGATATATGAATTAAATAACAAGAATAAATCGAGTCACCAAACCACTTGCCTTTTTTAACGAGACTTGTTCACCAACTTGATACAAAAGTCATTATCCTATGCAAATCAATAATCATACAAAAATATCCAATAACACTAAAAAATTAAAAGAAATGGATAATTTCACAATATGTAACTATATACTTAATTTATTGTTCTTATTTAGCTCAGTGGTTTGGTGAACGGAAAAAATTGCTCTGAACAAGTGGTTGAACTATGTTTTCAGTAATAGGATACGTTTAGTTATTAGTATGTTTTTATAGGTTATTGTGATTTTTTAATTTTCTTTACCTATTAAAGTGTTATACUBQ10 promoter6,3206,3406,3606,3806,4006,4206,4406,460
    TTATACGATAAAGAAGTTACTTTTCCAAGAAATTCACTGATTTTATAAGCCCACTTGCATTAGATAAATGGCAAAAAAAAACAAAAAGGAAAAGAAATAAAGCACGAAGAATTCTAGAAAATACGAAATACGCTTCAATGCAGTGGGACCCACGGTTCAATTATTGCCAATTTTCAATATGCTATTTCTTCAATGAAAAGGTTCTTTAAGTGACTAAAATATTCGGGTGAACGTAATCTATTTACCGTTTTTTTTTGTTTTTCCTTTTCTTTATTTCGTGCTTCTTAAGATCTTTTATGCTTTATGCGAAGTTACGTCACCCTGGGTGCCAAGTTAATAACGGTTAAAAGKflIEcoRIUBQ10 promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCTCCACCGTATATTTAAAAAATAAAACGATAATGCTAAAAAAATATAAATCGTAACGATCGTTAAATCTCAACGGCTGGATCTTATGACGACCGTTAGAAATTGTGGTTGTCGACGAGTCAGTAATAAACGGCGTCAAAGTGGTTGCAGCCGGCACACACGAGTCGTGTTTATTCGAGGTGGCATATAAATTTTTTATTTTGCTATTACGATTTTTTTATATTTAGCATTGCTAGCAATTTAGAGTTGCCGACCTAGAATACTGCTGGCAATCTTTAACACCAACAGCTGCTCAGTCATTATTTGCCGCAGTTTCACCAACGTCGGCCGTGTGTGCTCAGCACAAATASalIUBQ10 promoterUBQ10f ...inding6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CAACTCAAAGCACAAATACTTTTCCTCAACCTAAAAATAAGGCAATTAGCCAAAAACAACTTTGCGTGTAAACAACGCTCAATACACGTGTCATTTTATTATTAGCTATTGCTTCACCGCCTTAGCTTTCTCGTGACCTAGTCGTCCTCGTCTTTTCTTCTTCTTCTTCTATAAAGTTGAGTTTCGTGTTTATGAAAAGGAGTTGGATTTTTATTCCGTTAATCGGTTTTTGTTGAAACGCACATTTGTTGCGAGTTATGTGCACAGTAAAATAATAATCGATAACGAAGTGGCGGAATCGAAAGAGCACTGGATCAGCAGGAGCAGAAAAGAAGAAGAAGAAGATATTTUBQ10 promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ACAATACCCAAAGAGCTCTTCTTCTTCACAATTCAGATTTCAATTTCTCAAAATCTTAAAAACTTTCTCTCAATTCTCTCTACCGTGATCAAGGTAAATTTCTGTGTTCCTTATTCTCTCAAAATCTTCGATTTTGTTTTCGTTCGATCCCAATTTCGTATATGTTCTTTGGTTTTGTTATGGGTTTCTCGAGAAGAAGAAGTGTTAAGTCTAAAGTTAAAGAGTTTTAGAATTTTTGAAAGAGAGTTAAGAGAGATGGCACTAGTTCCATTTAAAGACACAAGGAATAAGAGAGTTTTAGAAGCTAAAACAAAAGCAAGCTAGGGTTAAAGCATATACAAGAAACCAAAUBQ10 promoter7,0207,0407,0607,0807,1007,1207,1407,160
    AGATTCTGTTAATCTTAGATCGAAGACGATTTTCTGGGTTTGATCGTTAGATATCATCTTAATTCTCGATTAGGGTTTCATAGATATCATCCGATTTGTTCAAATAATTTGAGTTTTGTCGAATAATTACTCTTCGATTTGTGATTTCTATCTAGATCTGGTGTTAGTTTCTAGTTCTAAGACAATTAGAATCTAGCTTCTGCTAAAAGACCCAAACTAGCAATCTATAGTAGAATTAAGAGCTAATCCCAAAGTATCTATAGTAGGCTAAACAAGTTTATTAAACTCAAAACAGCTTATTAATGAGAAGCTAAACACTAAAGATAGATCTAGACCACAATCAAAGATCAUBQ10 promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TTGTGCGATCGAATTTGTCGATTAATCTGAGTTTTTCTGATTAACAGGGATTCTAGAACTAGTGGATCTACAAAatgtctagagccccaaagaagaagagaaaagttagacccggggataagaaatactctattggtttggctatcggtacaaactctgttggttgggctgttatAACACGCTAGCTTAAACAGCTAATTAGACTCAAAAAGACTAATTGTCCCTAAGATCTTGATCACCTAGATGTTTtacagatctcggggtttcttcttctcttttcaatctgggcccctattctttatgagataaccaaaccgatagccatgtttgagacaaccaacccgacaataSmaISpeIXmaITspMIMS2RA4PK6KK8RK10VR12PG14DK16KY18SI20GL22AI24GT26NS28VG30WA32VI347425-11567A1P2K3K4K5R6K7V8R97434-7460dCas9UBQ10 promoterLinkerNLS7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tactgatgaatacaaggttccatccaagaagttcaaggttttgggtaacactgatagacactccatcaaaaagaacttgattggtgccttgttgttcgattctggtgaaactgctgaagctactagattgaaaagaaccgctagaagaagatacaccagaagaaagaacagaatcatgactacttatgttccaaggtaggttcttcaagttccaaaacccattgtgactatctgtgaggtagtttttcttgaactaaccacggaacaacaagctaagaccactttgacgacttcgatgatctaacttttcttggcgatcttcttctatgtggtcttctttcttgtcttagTD36EY38KV40PS42KK44FK46VL48GN50TD52RH54SI56KK58NL60IG62AL64LF66DS68GE70TA72EA74TR76LK78RT80AR82RR84YT86RR88KN90RI927425-11567dCas97,5407,5607,5807,6007,6207,6407,6607,6807,700
    tgctacttgcaagaaatcttctccaacgaaatggccaaggttgatgattcattcttccacagattggaagaatccttcttggtcgaagaagataagaagcacgaaagacatccaatcttcggtaacatcgttgatgaagttgcttaccacgaaaagtacccaactatctaccattacgatgaacgttctttagaagaggttgctttaccggttccaactactaagtaagaaggtgtctaaccttcttaggaagaaccagcttcttctattcttcgtgctttctgtaggttagaagccattgtagcaactacttcaacgaatggtgcttttcatgggttgatagatggtaaCY94LQ96EI98FS100NE102MA104KV106DD108SF110FH112RL114EE116SF118LV120EE122DK124KH126ER128HP130IF132GN134IV136DE138VA140YH142EK144YP146TI148YH1507425-11567dCas97,7207,7407,7607,7807,8007,8207,8407,860
    tgagaaagaagttggttgactctaccgataaggctgatttgagattgatctatttggctttggcccacatgattaagttcagaggtcatttcttgatcgaaggtgatttgaacccagataactccgatgttgataagttgttcatccaattagtccaaacctacaatcaattattactctttcttcaaccaactgagatggctattccgactaaactctaactagataaaccgaaaccgggtgtactaattcaagtctccagtaaagaactagcttccactaaacttgggtctattgaggctacaactattcaacaagtaggttaatcaggtttggatgttagttaataaLR152KK154LV156DS158TD160KA162DL164RL166IY168LA170LA172HM174IK176FR178GH180FL182IE184GD186LN188PD190NS192DV194DK196LF198IQ200LV202QT204YN206QL208F7425-11567dCas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    cgaagaaaacccaatcaacgcctctggtgttgatgctaaagctattttgtctgccagattgtccaagtccagaagattagaaaatttgatcgcccaattaccaggtgaaaagaagaatggtttgttcggtaatttgattgccttgtctttgggtttgactccaaacttcaagtccgcttcttttgggttagttgcggagaccacaactacgatttcgataaaacagacggtctaacaggttcaggtcttctaatcttttaaactagcgggttaatggtccacttttcttcttaccaaacaagccattaaactaacggaacagaaacccaaactgaggtttgaagttcaggE210EN212PI214NA216SG218VD220AK222AI224LS226AR228LS230KS232RR234LE236NL238IA240QL242PG244EK246KN248GL250FG252NL254IA256LS258LG260LT262PN264FK266S7425-11567dCas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    aatttcgatttggctgaagatgccaagttgcaattatctaaggatacctacgatgacgatttggataacttgttggctcaaatcggtgatcaatacgctgatttgtttttggctgctaagaacttgtccgatgccattttgttgtccgatattttgagagtcaacaccgaaattattaaagctaaaccgacttctacggttcaacgttaatagattcctatggatgctactgctaaacctattgaacaaccgagtttagccactagttatgcgactaaacaaaaaccgacgattcttgaacaggctacggtaaaacaacaggctataaaactctcagttgtggctttaatN268FD270LA272ED274AK276LQ278LS280KD282TY284DD286DL288DN290LL292AQ294IG296DQ298YA300DL302FL304AA306KN308LS310DA312IL314LS316DI318LR320VN322TE324I7425-11567dCas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    ctaaggctccattgtctgcctctatgatcaaaagatacgatgaacaccaccaagacttgactttgttgaaggctttggtcagacaacaattacctgaaaagtacaaagaaattttcttcgatcaatccaagaacggttacgccggttatattgatggtggtgcttctcaagaagagattccgaggtaacagacggagatactagttttctatgctacttgtggtggttctgaactgaaacaacttccgaaaccagtctgttgttaatggacttttcatgtttctttaaaagaagctagttaggttcttgccaatgcggccaatataactaccaccacgaagagttcttctT326KA328PL330SA332SM334IK336RY338DE340HH342QD344LT346LL348KA350LV352RQ354QL356PE358KY360KE362IF364FD366QS368KN370GY372AG374YI376DG378GA380SQ382EE3847425-11567dCas98,4208,4408,4608,4808,5008,5208,5408,560
    attttacaagttcatcaagccaatcttggaaaagatggacggtactgaagaattattggtcaagttgaacagagaagatttgttgagaaagcaaagaaccttcgacaacggttctattccacatcaaattcacttgggtgaattgcacgcaattttgagaagacaagaagatttttaaaatgttcaagtagttcggttagaaccttttctacctgccatgacttcttaataaccagttcaacttgtctcttctaaacaactctttcgtttcttggaagctgttgccaagataaggtgtagtttaagtgaacccacttaacgtgcgttaaaactcttctgttcttctaaaaFY386KF388IK390PI392LE394KM396DG398TE400EL402LV404KL406NR408ED410LL412RK414QR416TF418DN420GS422IP424HQ426IH428LG430EL432HA434IL436RR438QE440DF4427425-11567dCas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    tatccattcttgaaggacaacagagaaaagatcgaaaagattctgaccttcagaatcccttactacgttggtccattggctagaggtaattcaagatttgcctggatgactagaaagtccgaagaaactattactccttggaacttcgaagaagttgtagataagggtgcttctgataggtaagaacttcctgttgtctcttttctagcttttctaagactggaagtcttagggaatgatgcaaccaggtaaccgatctccattaagttctaaacggacctactgatctttcaggcttctttgataatgaggaaccttgaagcttcttcaacatctattcccacgaagacYP444FL446KD448NR450EK452IE454KI456LT458FR460IP462YY464VG466PL468AR470GN472SR474FA476WM478TR480KS482EE484TI486TP488WN490FE492EV494VD496KG498AS5007425-11567dCas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    cccaatcctttattgaaagaatgaccaacttcgacaagaacttgccaaacgaaaaggttttgccaaagcactctttgttgtacgaatacttcaccgtctacaacgaattgactaaggttaagtacgtcaccgaaggtatgagaaaaccagcttttttatccggtgaacaaaagaagggttaggaaataactttcttactggttgaagctgttcttgaacggtttgcttttccaaaacggtttcgtgagaaacaacatgcttatgaagtggcagatgttgcttaactgattccaattcatgcagtggcttccatactcttttggtcgaaaaaataggccacttgttttcttBarIAQ502SF504IE506RM508TN510FD512KN514LP516NE518KV520LP522KH524SL526LY528EY530FT532VY534NE536LT538KV540KY542VT544EG546MR548KP550AF552LS554GE556QK558K7425-11567dCas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggctatcgtcgatttgttgttcaagaccaacagaaaggttactgtcaagcaattaaaagaagattacttcaagaaaatcgaatgcttcgactccgttgaaatttctggtgtcgaagatagattcaatgcctctttaggtacttaccatgacttgttgaaaatcatcaaggacaagccgatagcagctaaacaacaagttctggttgtctttccaatgacagttcgttaattttcttctaatgaagttcttttagcttacgaagctgaggcaactttaaagaccacagcttctatctaagttacggagaaatccatgaatggtactgaacaacttttagtagttcctgttcA560IV562DL564LF566KT568NR570KV572TV574KQ576LK578ED580YF582KK584IE586CF588DS590VE592IS594GV596ED598RF600NA602SL604GT606YH608DL610LK612II614KD616K7425-11567dCas99,1209,1409,1609,1809,2009,2209,2409,260
    gatttcttggacaacgaagaaaacgaagatattttggaagatattgtcttgacattgaccttgtttgaagatagagaaatgattgaagaaagattgaaaacctacgcccacttgttcgatgataaggttatgaagcaattaaagagaagaagatacactggttggggtagattgtctaaagaacctgttgcttcttttgcttctataaaaccttctataacagaactgtaactggaacaaacttctatctctttactaacttctttctaacttttggatgcgggtgaacaagctactattccaatacttcgttaatttctcttcttctatgtgaccaaccccatctaacaD618FL620DN622EE624NE626DI628LE630DI632VL634TL636TL638FE640DR642EM644IE646ER648LK650TY652AH654LF656DD658KV660MK662QL664KR666RR668YT670GW672GR674L7425-11567dCas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    ccagaaaattgattaacggtatcagagacaagcaatccggtaagaccattttggactttttgaagtctgatggtttcgctaacagaaacttcatgcaattaatccacgacgattccttgactttcaaagaagatatacaaaaggcccaagtctctggtcaaggtgattctttacaggtcttttaactaattgccatagtctctgttcgttaggccattctggtaaaacctgaaaaacttcagactaccaaagcgattgtctttgaagtacgttaattaggtgctgctaaggaactgaaagtttcttctatatgttttccgggttcagagaccagttccactaagaaatgtS676RK678LI680NG682IR684DK686QS688GK690TI692LD694FL696KS698DG700FA702NR704NF706MQ708LI710HD712DS714LT716FK718ED720IQ722KA724QV726SG728QG730DS732LH7347425-11567dCas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    tgaacatatcgctaacttggctggttctccagctattaagaagggtattttacaaaccgttaaggtcgttgacgaattggtcaaagttatgggtagacataagccagaaaacatcgttatcgaaatggctagagaaaatcaaaccacccaaaagggtcaaaagaactccagagaaacttgtatagcgattgaaccgaccaagaggtcgataattcttcccataaaatgtttggcaattccagcaactgcttaaccagtttcaatacccatctgtattcggtcttttgtagcaatagctttaccgatctcttttagtttggtgggttttcccagttttcttgaggtctcttEH736IA738NL740AG742SP744AI746KK748GI750LQ752TV754KV756VD758EL760VK762VM764GR766HK768PE770NI772VI774EM776AR778EN780QT782TQ784KG786QK788NS790RE7927425-11567dCas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    agaatgaagagaatcgaagaaggtatcaaagaattgggttcccaaattttgaaagaacacccagttgaaaacacccaattacaaaacgaaaagttgtacttgtactacttgcaaaacggtagagatatgtacgttgaccaagaattggacatcaacagattgtctgattacgatgtcttacttctcttagcttcttccatagtttcttaacccaagggtttaaaactttcttgtgggtcaacttttgtgggttaatgttttgcttttcaacatgaacatgatgaacgttttgccatctctatacatgcaactggttcttaacctgtagttgtctaacagactaatgctacRM794KR796IE798EG800IK802EL804GS806QI808LK810EH812PV814EN816TQ818LQ820NE822KL824YL826YY828LQ830NG832RD834MY836VD838QE840LD842IN844RL846SD848YD8507425-11567dCas99,8209,8409,8609,8809,9009,9209,9409,960
    ttgacgctatcgttccacaatcttttttgaaggatgactccattgacaacaaggtcttgactagatccgataagaatagaggtaagtccgataacgttccatctgaagaagtcgttaagaaaatgaagaactattggagacaattattgaacgccaagttgatcacccaaagaaaaactgcgatagcaaggtgttagaaaaaacttcctactgaggtaactgttgttccagaactgatctaggctattcttatctccattcaggctattgcaaggtagacttcttcagcaattcttttacttcttgataacctctgttaataacttgcggttcaactagtgggtttctttVD852AI854VP856QS858FL860KD862DS864ID866NK868VL870TR872SD874KN876RG878KS880DN882VP884SE886EV888VK890KM892KN894YW896RQ898LL900NA902KL904IT906QR908K7425-11567dCas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    gtttgacaatttgaccaaggctgaaagaggtggtttgtctgaattggataaggcaggtttcatcaaaagacaattagtagaaaccagacaaatcaccaagcacgttgctcaaattttggatagtagaatgaacactaagtacgacgaaaacgacaaattgatcagagaagttaagcaaactgttaaactggttccgactttctccaccaaacagacttaacctattccgtccaaagtagttttctgttaatcatctttggtctgtttagtggttcgtgcaacgagtttaaaacctatcatcttacttgtgattcatgctgcttttgctgtttaactagtctcttcaattcF910DN912LT914KA916ER918GG920LS922EL924DK926AG928FI930KR932QL934VE936TR938QI940TK942HV944AQ946IL948DS950RM952NT954KY956DE958ND960KL962IR964EV966K7425-11567dCas910,16010,18010,20010,22010,24010,26010,28010,30010,320
    gtcattaccttgaagtccaagttggtttccgatttcagaaaggacttccaattctacaaggtcagagaaatcaacaactaccatcatgcacatgatgcttacttgaatgctgttgttggtactgccttgattaagaagtatccaaagttggaatccgaatttgtctacggtgattcagtaatggaacttcaggttcaaccaaaggctaaagtctttcctgaaggttaagatgttccagtctctttagttgttgatggtagtacgtgtactacgaatgaacttacgacaacaaccatgacggaactaattcttcataggtttcaaccttaggcttaaacagatgccactaaV968IT970LK972SK974LV976SD978FR980KD982FQ984FY986KV988RE990IN992NY994HH996AH998DA1000YL1002NA1004VV1006GT1008AL1010IK1012KY1014PK1016LE1018SE1020FV1022YG1024D7425-11567dCas910,34010,36010,38010,40010,42010,44010,46010,48010,500
    acaaggtttacgacgttagaaagatgatcgccaagtccgaacaagaaattggtaaagctactgccaaatacttcttctactccaatattatgaatttctttaagaccgaaatcactttggccaacggtgaaattagaaaaagaccattgattgaaactaatggtgaaacaggtgatgttccaaatgctgcaatctttctactagcggttcaggcttgttctttaaccatttcgatgacggtttatgaagaagatgaggttataatacttaaagaaattctggctttagtgaaaccggttgccactttaatctttttctggtaactaactttgattaccactttgtccactY1026KV1028YD1030VR1032KM1034IA1036KS1038EQ1040EI1042GK1044AT1046AK1048YF1050FY1052SN1054IM1056NF1058FK1060TE1062IT1064LA1066NG1068EI1070RK1072RP1074LI1076ET1078NG1080ET1082GE10847425-11567dCas910,52010,54010,56010,58010,60010,62010,64010,660
    aatcgtttgggataagggtagagattttgccactgttagaaaggtattgtccatgccacaagtaaacatcgtcaaaaagaccgaagttcaaactggtggtttctccaaagaatccattttgcctaagagaaactccgataagttgatcgctagaaaaaaagactgggacccaaaattagcaaaccctattcccatctctaaaacggtgacaatctttccataacaggtacggtgttcatttgtagcagtttttctggcttcaagtttgaccaccaaagaggtttcttaggtaaaacggattctctttgaggctattcaactagcgatctttttttctgaccctgggttttKflIIV1086WD1088KG1090RD1092FA1094TV1096RK1098VL1100SM1102PQ1104VN1106IV1108KK1110TE1112VQ1114TG1116GF1118SK1120ES1122IL1124PK1126RN1128SD1130KL1132IA1134RK1136KD1138WD1140PK11427425-11567dCas910,68010,70010,72010,74010,76010,78010,80010,82010,840
    aagtacggtggttttgattctccaactgttgcttactctgttttggttgttgctaaggtcgaaaagggtaagagtaagaagttgaagtccgtcaaagaattattaggtatcactatcatggaaagatcctcattcgaaaagaatcctatcgactttttggaagccaagggttacattcatgccaccaaaactaagaggttgacaacgaatgagacaaaaccaacaacgattccagcttttcccattctcattcttcaacttcaggcagtttcttaataatccatagtgatagtacctttctaggagtaagcttttcttaggatagctgaaaaaccttcggttcccaatgtKY1144GG1146FD1148SP1150TV1152AY1154SV1156LV1158VA1160KV1162EK1164GK1166SK1168KL1170KS1172VK1174EL1176LG1178IT1180IM1182ER1184SS1186FE1188KN1190PI1192DF1194LE1196AK1198GY12007425-11567dCas910,86010,88010,90010,92010,94010,96010,98011,00011,020
    aagaagtcaagaaggacttgatcattaagttgccaaagtacagtttgttcgaattggaaaatggtagaaagagaatgttggcttctgccggtgaattacaaaagggtaatgaattggctttgccatccaagtacgttaatttcttatacttggcctcccactacgaaaaattgaattcttcagttcttcctgaactagtaattcaacggtttcatgtcaaacaagcttaaccttttaccatctttctcttacaaccgaagacggccacttaatgttttcccattacttaaccgaaacggtaggttcatgcaattaaagaatatgaaccggagggtgatgctttttaacttKE1202VK1204KD1206LI1208IK1210LP1212KY1214SL1216FE1218LE1220NG1222RK1224RM1226LA1228SA1230GE1232LQ1234KG1236NE1238LA1240LP1242SK1244YV1246NF1248LY1250LA1252SH1254YE1256KL1258K7425-11567dCas911,04011,06011,08011,10011,12011,14011,16011,18011,200
    aggttctcctgaagataacgaacaaaagcaattatttgtcgaacaacacaagcactacttggacgaaatcattgaacaaatttccgaattttccaaaagagtcattttggctgacgccaatttggacaaagttttgtcagcttacaacaagcacagagataagccaattagagaatccaagaggacttctattgcttgttttcgttaataaacagcttgttgtgttcgtgatgaacctgctttagtaacttgtttaaaggcttaaaaggttttctcagtaaaaccgactgcggttaaacctgtttcaaaacagtcgaatgttgttcgtgtctctattcggttaatctcttG1260SP1262ED1264NE1266QK1268QL1270FV1272EQ1274HK1276HY1278LD1280EI1282IE1284QI1286SE1288FS1290KR1292VI1294LA1296DA1298NL1300DK1302VL1304SA1306YN1308KH1310RD1312KP1314IR1316E7425-11567dCas911,22011,24011,26011,28011,30011,32011,34011,360
    caagctgaaaacatcattcacttgttcactttgactaacttgggtgctccagctgcttttaagtatttcgataccactatcgacagaaagagatacacctctaccaaagaagttttggacgctactttgatccaccaatctattactggtttgtacgaaactagaatcgacttgtgttcgacttttgtagtaagtgaacaagtgaaactgattgaacccacgaggtcgacgaaaattcataaagctatggtgatagctgtctttctctatgtggagatggtttcttcaaaacctgcgatgaaactaggtggttagataatgaccaaacatgctttgatcttagctgaacaQ1318AE1320NI1322IH1324LF1326TL1328TN1330LG1332AP1334AA1336FK1338YF1340DT1342TI1344DR1346KR1348YT1350ST1352KE1354VL1356DA1358TL1360IH1362QS1364IT1366GL1368YE1370TR1372ID1374L7425-11567dCas911,38011,40011,42011,44011,46011,48011,50011,52011,540
    ctcaattaggtggtgatGGTTCTGGATCAGGTAGTGGCTCAGGATCTAAGAGAGGTTTCTCAGAGACTGTTGACTTGAAACTTAATCTGAACAATGAGCCGGCTAACAAGGAGGGGTCTACCACTCATGATGTTGTTACATTTGATAGTAAAGAGAAGTCGGCCTGTCCTAAGGAgagttaatccaccactaCCAAGACCTAGTCCATCACCGAGTCCTAGATTCTCTCCAAAGAGTCTCTGACAACTGAACTTTGAATTAGACTTGTTACTCGGCCGATTGTTCCTCCCCAGATGGTGAGTACTACAACAATGTAAACTATCATTTCTCTTCAGCCGGACAGGATTCCTBsu36IS1376QL1378GG1380D7425-11567K1R2G3F4S5E6T7V8D9L10K11L12N13L14N15N16E17P18A19N20K21E22G23S24T25T26H27D28V29V30T31F32D33S34K35E36K37S38A39C40P41K42D4311598-11840NdC_IAA17-CodonOpt1L1dCas911,56011,58011,60011,62011,64011,66011,68011,70011,720
    CCCTGCCAAACCGCCTGCAAAAGCCCAAGTGGTGGGTTGGCCTCCGGTGAGGTCTTATAGGAAAAATGTAATGGTGTCATGCCAAAAGAGTTCTGGAGGTCCTGAAGCTGCGGCCGctgcaggaggcggtggaagcATGTCTTCTCTTAGTAGAGAGCTCGTTTTCTTGATCTTAGGGACGGTTTGGCGGACGTTTTCGGGTTCACCACCCAACCGGAGGCCACTCCAGAATATCCTTTTTACATTACCACAGTACGGTTTTCTCAAGACCTCCAGGACTTCGACGCCGGCgacgtcctccgccaccttcgTACAGAAGAGAATCATCTCTCGAGCAAAAGAACTAGAATP44A45K46P47P48A49K50A51Q52V53V54G55W56P57P58V59R60S61Y62R63K64N65V66M67V68S69C70Q71K72S73S74G75G76P77E78A79A80A8111598-11840NdC_IAA17-CodonOpt1TPLRD2F primerL2F primer11,74011,76011,78011,80011,82011,84011,86011,88011,900
    CAGTTTCTCGATGAAGAGAAGTTTAAAGAGACTGTTCACAAGCTTGAACAAGAATCTGGGTTTTTCTTCAATATGAAGTATTTTGAGGATGAGGTTCACAATGGAAATTGGGATGAGGTTGAGAAGTATCTCTCTGGTTTCACTAAAGTAGATGATAATAGATACTCTATGAAGAGTCAAAGAGCTACTTCTCTTCAAATTTCTCTGACAAGTGTTCGAACTTGTTCTTAGACCCAAAAAGAAGTTATACTTCATAAAACTCCTACTCCAAGTGTTACCTTTAACCCTACTCCAACTCTTCATAGAGAGACCAAAGTGATTTCATCTACTATTATCTATGAGATACTTCTHindIIITPLRD2CTLH domain11,92011,94011,96011,98012,00012,02012,04012,060
    TATTCTTCGAGATAAGAAAGCAGAAGTATCTCGAGGCTTTGGATAAGCATGATCGTCCCAAGGCTGTGGATATACTAGTGAAGGATTTGAAAGTGTTTTCAACTTTTAATGAGGAGCTTTTCAAGGAAATAACACAGCTCTTGACATTGGAGAACTTCCGGGAGAATGAACAGTTATAAGAAGCTCTATTCTTTCGTCTTCATAGAGCTCCGAAACCTATTCGTACTAGCAGGGTTCCGACACCTATATGATCACTTCCTAAACTTTCACAAAAGTTGAAAATTACTCCTCGAAAAGTTCCTTTATTGTGTCGAGAACTGTAACCTCTTGAAGGCCCTCTTACTTGTCAASpeITPLRD2CTLH domain12,08012,10012,12012,14012,16012,18012,20012,22012,240
    ATCCAAGTATGGGGACACCAAGTCTGCAAGAGCGATCATGTTGGTGGAACTCAAAAAGCTGATTGAGGCGAATCCTTTATTCCGTGATAAATTGCAGTTCCCTACTCTTAGAAATTCAAGGCTGAGGACTTTGATCAACCAGAGCTTAAATTGGCAACACCAGCTTTGTAAAAATTAGGTTCATACCCCTGTGGTTCAGACGTTCTCGCTAGTACAACCACCTTGAGTTTTTCGACTAACTCCGCTTAGGAAATAAGGCACTATTTAACGTCAAGGGATGAGAATCTTTAAGTTCCGACTCCTGAAACTAGTTGGTCTCGAATTTAACCGTTGTGGTCGAAACATTTTTABbvCITPLRD212,26012,28012,30012,32012,34012,36012,38012,40012,420
    CCAAGGCCAAATCCAGATATCAAGACTCTTTTTGTGGATCACTCTTGTGGCCCTCCAAATGGCGCACGAGCTCCATCTCCTGTGAACAATCCACTGCTTGGAGGGATACCAAAGGCTGGAGGATTTCCTCCTTTAGGCGCACACGGGCCATTTCAACCAACGGCGTCACCTGTTCGGTTCCGGTTTAGGTCTATAGTTCTGAGAAAAACACCTAGTGAGAACACCGGGAGGTTTACCGCGTGCTCGAGGTAGAGGACACTTGTTAGGTGACGAACCTCCCTATGGTTTCCGACCTCCTAAAGGAGGAAATCCGCGTGTGCCCGGTAAAGTTGGTTGCCGCAGTGGACAAGTPLRD2Pro-rich12,44012,46012,48012,50012,52012,54012,56012,58012,600
    CAACACCTCTTGCTGGTTGGATGTCTAGTCCATCCTCTGTCCCACATCCAGCTGTCTCAGCAGGAGCCATTGCACTTGGTGGTCCATCAATCCCAGCGGCGTTGAAGCACCCGAGAACTCCTCCAACTAATGCTTCTTTAGACTACCCGTCAGCAGATTCTtagtgaCACGTGcgGTTGTGGAGAACGACCAACCTACAGATCAGGTAGGAGACAGGGTGTAGGTCGACAGAGTCGTCCTCGGTAACGTGAACCACCAGGTAGTTAGGGTCGCCGCAACTTCGTGGGCTCTTGAGGAGGTTGATTACGAAGAAATCTGATGGGCAGTCGTCTAAGAatcactGTGCACgcDraIIITPLRD2Pro-rich12,62012,64012,66012,68012,70012,72012,74012,760
    ttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacaagtttgtaaaccgttatttcaaagaattctaacttaggacaacggccagaacgctactaatagtatattaaagacaacttaatgcaattcgtacattattaattgtacattacgtactgcaataaatactctacccaaaaatactaatctcagggcgttaatatgtaaattatgnos (nopaline synthase) 3'UTR (polyA signal)12,78012,80012,82012,84012,86012,88012,90012,92012,940
    gcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggCCTGCAGGACGCGTGGCGCGCCagatccAATTAAACTATCAGTGTTTGACAGGATATATTGGcgggtaaacctaagagaaaagagcgtttattagaatacgctatcttttgttttatatcgcgcgtttgatcctatttaatagcgcgcgccacagtagatacaatgatctagcccGGACGTCCTGCGCACCGCGCGGtctaggTTAATTTGATAGTCACAAACTGTCCTATATAACCgcccatttggattctcttttctcgcaaataatcttatAscISbfIMauBInos (nopaline synthase) 3'UTR (polyA signal)right bord...NA repeatRareCut-...scISbfI12,96012,98013,00013,02013,04013,06013,08013,10013,120
    acggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtgcctataaattttcccgcacttttccaaataggcaagcaggtaaacatacacgtacggttggtgtcccaaggggagccctagtttcatgaaactaggttggggaggcgacgatatcacgtcagccgaagactgcaagtcacgtcggcagaagacttttgctgtacagcgtgttc13,14013,16013,18013,20013,22013,24013,26013,28013,300
    tcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggactaggattcaatgcgctgtccgacggcgggacgggaaaaggaccgcaaaagaacagcgcacaaaatcagcgtatttcatcttatgaacgctgatcttggcctctgtaatgcggtacttgttctcgcggcggcgaccggacgacccgatacgggcgcagtcgtggctgctggtcctga13,32013,34013,36013,38013,40013,42013,44013,460
    tgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggttggttgcccggcttgacgtgcgccggccgacgtggttcgacaaaaggctcttctagtggccgtggtccgcgctggcgggcctcgaccggtcctacgaactggtggatgcgggaccgctgcaacactgtcactggtccgatctggcggaccgggcgtcgtgggcgctgga13,48013,50013,52013,54013,56013,58013,60013,62013,640
    actggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgcctgacctgtaacggctcgcgtaggtcctccggccgcgcccggacgcatcggaccgtctcggcacccggctgtggtggtgcggccggccggcgtaccacaactggcacaagcggccgtaacggctcaagctcgcaagggattagtagctggcgtgggcctcgcccgcgctccggcgg13,66013,68013,70013,72013,74013,76013,78013,80013,820
    aaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccattccgggctccgcacttcaaaccgggggcgggatgggagtggggccgtgtctagcgcgtgcgggcgctcgactagctggtccttccggcgtggcactttctccgccgacgtgacgaaccgcacgtagcgagctgggacatggcgcgtgaactcgcgtcgctccttcactgcgggt13,84013,86013,88013,90013,92013,94013,96013,98014,000
    ccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacggctccggtccgccgcgccacggaaggcactcctgcgtaactggctccggctgcgggaccgccggcggctcttacttgcggttctccttgttcgtactttggcgtggtcctgccggtcctgcttggcaaaaagtaatggcttctctagctccgcctctactagcgccggcccatgSTA re...asmid14,02014,04014,06014,08014,10014,12014,14014,160
    gtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcacaagctcggcgggcgcgtgcagagttggcacgccgacgtactttaggaccggccaaacagactacggttcgaccgccggaccggccggtcgaaccggcgacttctttggctcgcggcggcagatttttccactacacataaactcattttgtcgaacgcagtacgccagcgacSTA region from pVS1 plasmid14,18014,20014,22014,24014,26014,28014,30014,32014,340
    cgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcatatactacgctactcatttatttgtttatgcgttccccttgcgtacttccaatagcgacatgaattggtctttccgcccagtccgttctgctggtagcgttgggtagatcgggcgcgggacgttgagcggccccggctacaagacaatcagctaaggctaggggtcccgtcaSTA region from pVS1 plasmid14,36014,38014,40014,42014,44014,46014,48014,50014,520
    gcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgccgggcgctaacccgccggcacgcccttctagttggcgattggcaacagccgtagctggcgggctgctaactggcgctgcacttccggtagccggccgcgctgaagcatcactagctgcctcgcggggtccgccgcctgaaccgacacaggcgctagttccgtcggctgaagcacgSTA region from pVS1 plasmid14,54014,56014,58014,60014,62014,64014,66014,68014,700
    tgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgaactaaggccacgtcggttcgggaatgctgtatacccggtggcggctggaccacctcgaccaattcgtcgcgtaactccagtgcctaccttccgatgttcgccggaaacagcacagcgcccgctagtttccgtgcgcgtagccgccactccaacggctccgcgaccggcccatgctSTA region from pVS1 plasmid14,72014,74014,76014,78014,80014,82014,84014,860
    gctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgacgacgggtaagaactcagggcatagtgcgtcgcgcactcgatgggtccgtgacggcggcggccgtgttggcaagaacttagtcttgggctcccgctgcgacgggcgctccaggtccgcgaccggcgactttaatttagttttgagtaaactcaattactccatttctcttttactSTA region from pVS1 plasmid14,88014,90014,92014,94014,96014,98015,00015,02015,040
    gcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgacgttttcgtgtttgtgcgattcacggccggcaggctcgcgtgcgtcgtcgttccgacgttgcaaccggtcggaccgtctgtgcggtcggtacttcgcccagttgaaagtcaacggccgcctcctagtgtggttcgacttctacatgcgccatgcggttccgttctggtaatggctSTA region from pVS1 plasmid15,06015,08015,10015,12015,14015,16015,18015,20015,220
    gctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgcgacgatagacttatgtagcgcgtcgatggtctcatttactcgtttacttatttactcatctacttaaaatcgccgatttcctccgccgtaccttttagttcttgttggtccgtggctgcggcaccttacggggtacacacctccttgcccgccaaccggtccgcattcgccgac15,24015,26015,28015,30015,32015,34015,36015,38015,400
    ggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgacggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgcccccaacagacggccgggacgttaccgtgaccttgggggttcgggctccttagccgcactgccagcgtttggtaggccgggccatgtttagccgcgccgcgacccactactggaccacctcttcaacttccggcgcgtccggcgggtcgccgttgcgtagctccgtcttcgtgcggg15,42015,44015,46015,48015,50015,52015,54015,560
    cggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgccacttagcaccgttcgccggcgactagcttaggcgtttcttagggccgttggcggccgtcggccacgcggcagctaatccttcggcgggttcccgctgctcgttggtctaaaaaagcaaggctacgagatactgcacccgtgggcgctatcagcgtcgtagtacctgcaccgg15,58015,60015,62015,64015,66015,68015,70015,72015,740
    gttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagcaaaaggcagacagcttcgcactggctgctcgaccgctccactaggcgatgctcgaaggtctgcccgtgcatctccaaaggcgtcccggccggccgtaccggtcacacaccctaatgctggaccatgactaccgccaaagggtagattggcttaggtacttggctatggcccttcpVS1-REP; replication origin from pVS115,76015,78015,80015,82015,84015,86015,88015,90015,920
    ggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtccttccctctgttcgggccggcgcacaaggcaggtgtgcaacgcctgcatgagttcaagacggccgctcggctaccgcctttcgtctttctgctggaccatctttggacgtaagccaatttgtggtgcgtgcaacggtacgtcgcatgcttcttccggttcttgccggcggaccaBsiWIpVS1-REP; replication origin from pVS115,94015,96015,98016,00016,02016,04016,06016,08016,100
    gacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcctgccataggctcccacttcggaactaatcggcgatgttctagcatttctcgctttggcccgccggcctcatgtagctctagctcgatcgactaacctacatggcgctctagtgtcttccgttcttgggcctgcacgactgccaagtggggctaatgaaaaactagctagggccgBmtINheIpVS1-REP; replication origin from pVS116,12016,14016,16016,18016,20016,22016,24016,260
    atcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggtagccggcaaaagagatggcggaccgtgcggcgcggcgtccgttccgtcttcggtctaccaacaagttctgctagatgcttgcgtcaccgtcgcggcctctcaagttcttcaagacaaagtggcacgcgttcgactagcccagtttactggacggcctcatgctaaacttcctccFspIpVS1-REP; replication origin from pVS116,28016,30016,32016,34016,36016,38016,40016,42016,440
    aggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattgggaacccaaagcctccgccccgtccgaccgggctaggatcagtacgcgatggcgttggactagctcccgcttcgtaggcggccaaggattacatgcctcgtctacgatcccgtttaacgggatcgtcccctttttccagcttttccagagaaaggacacctatcgtgcatgtaacccttgggtttcggBsaIpVS1-REP; replication origin from pVS116,46016,48016,50016,52016,54016,56016,58016,60016,620
    gtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcatgtaacccttggccttgggcatgtaacccttgggtttcggcatgtaacccttggccagtgtgtacattcactgactatattttctcttttttccgctaaaaaggcggattttgagaaattttgaataattttgagaattttgggcggaccggacacgtattgacagaccggtcpVS1-REP; replication origin from pVS116,64016,66016,68016,70016,72016,74016,76016,78016,800
    cgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgccgcgtgtcggcttctcgacgtttttcgcggatgggaagccagcgacgcgagggatgcggggcggcgaagcgcagccggatagcgccggcgaccggcgagtttttaccgaccggatgccggtccgttagatggtcccgcgcctgttcggcgcggcagcggtgagctggcggccgcggMreI16,82016,84016,86016,88016,90016,92016,94016,960
    cacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtgtagttccgtgggacggagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgcgtcggtactgggtcagtg16,98017,00017,02017,04017,06017,08017,10017,12017,140
    gtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcatcgctatcgcctcacatatgaccgaattgatacgccgtagtctcgtctaacatgactctcacgtggtatacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccBstZ17Ibom site from pBR32217,16017,18017,20017,22017,24017,26017,28017,30017,320
    cgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgbom site from pBR32217,34017,36017,38017,40017,42017,44017,46017,48017,500
    gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcpBR322 origin of replication17,52017,54017,56017,58017,60017,62017,64017,660
    ttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccapBR322 origin of replication17,68017,70017,72017,74017,76017,78017,80017,82017,840
    gctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgccgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcg17,86017,88017,90017,92017,94017,96017,98018,00018,020
    gcagaaaaaaaggatctcaagaagaattgcgaccgtcgaggagaacttctagtatatctacatacctaatattattgccttattaaaaatggaatcccaacaattacatcaaaatccacattctcttcaaaatcaattgtcctgtacttccttgttcatgtgtgttcaaaaacgtcgtctttttttcctagagttcttcttaacgctggcagctcctcttgaagatcatatagatgtatggattataataacggaataatttttaccttagggttgttaatgtagttttaggtgtaagagaagttttagttaacaggacatgaaggaacaagtacacacaagtttttgcaLEU2 Promoter18,04018,06018,08018,10018,12018,14018,16018,18018,200
    tatatttataggataattatactctatttctcaacaagtaattggttgtttggccgagcggtctaaggcgcctgattcaagaaatatcttgaccgcagttaactgtgggaatactcaggtatcgtaagatgcaagagttcgaatctcttagcaaccattatttttttcctcaacaatataaatatcctattaatatgagataaagagttgttcattaaccaacaaaccggctcgccagattccgcggactaagttctttatagaactggcgtcaattgacacccttatgagtccatagcattctacgttctcaagcttagagaatcgttggtaataaaaaaaggagttgtHpaILEU2 Promoter18,22018,24018,26018,28018,30018,32018,34018,360
    taacgagaacacacaggggcgctatcgcacagaatcaaattcgatgactggaaattttttgttaatttcagaggtcgcctgacgcatatacctttttcaactgaaaaattgggagaaaaaggaaaggtgagagcgccggaaccggcttttcatatagaatagagaagcgttcatgattgctcttgtgtgtccccgcgatagcgtgtcttagtttaagctactgacctttaaaaaacaattaaagtctccagcggactgcgtatatggaaaaagttgactttttaaccctctttttcctttccactctcgcggccttggccgaaaagtatatcttatctcttcgcaagtacLEU2 Promoter18,38018,40018,42018,44018,46018,48018,50018,52018,540
    actaaatgcttgcatcacaatacttgaagttgacaatattatttaaggacctattgttttttccaataggtggttagcaatcgtcttactttctaacttttcttaccttttacatttcagcaatatatatatatatatttcaaggatataccattctaatgtctgcccctaagaatgatttacgaacgtagtgttatgaacttcaactgttataataaattcctggataacaaaaaaggttatccaccaatcgttagcagaatgaaagattgaaaagaatggaaaatgtaaagtcgttatatatatatatataaagttcctatatggtaagattacagacggggattcttLEU2 PromoterLEU218,56018,58018,60018,62018,64018,66018,68018,70018,720
    gatcgtcgttttgccaggtgaccacgttggtcaagaaatcacagccgaagccattaaggttcttaaagctatttctgatgttcgttccaatgtcaagttcgatttcgaaaatcatttaattggtggtgctgctatcgatgctacaggtgttccacttccagatgaggcgctggaactagcagcaaaacggtccactggtgcaaccagttctttagtgtcggcttcggtaattccaagaatttcgataaagactacaagcaaggttacagttcaagctaaagcttttagtaaattaaccaccacgacgatagctacgatgtccacaaggtgaaggtctactccgcgaccttLEU218,74018,76018,78018,80018,82018,84018,86018,88018,900
    gcctccaagaaggctgatgccgttttgttaggtgctgtgggtggtcctaaatggggaaccggtagtgttagacctgaacaaggtttactaaaaatccgtaaagaacttcaattgtacgccaacttaagaccatgtaactttgcatccgactctcttttagacttatctccaatcacggaggttcttccgactacggcaaaacaatccacgacacccaccaggatttaccccttggccatcacaatctggacttgttccaaatgatttttaggcatttcttgaagttaacatgcggttgaattctggtacattgaaacgtaggctgagagaaaatctgaatagaggttagtLEU218,92018,94018,96018,98019,00019,02019,04019,060
    agccacaatttgctaaaggtactgacttcgttgttgtcagagaattagtgggaggtatttactttggtaagagaaaggaagacgatggtgatggtgtcgcttgggatagtgaacaatacaccgttccagaagtgcaaagaatcacaagaatggccgctttcatggccctacaacatcggtgttaaacgatttccatgactgaagcaacaacagtctcttaatcaccctccataaatgaaaccattctctttccttctgctaccactaccacagcgaaccctatcacttgttatgtggcaaggtcttcacgtttcttagtgttcttaccggcgaaagtaccgggatgttgtLEU219,08019,10019,12019,14019,16019,18019,20019,22019,240
    tgagccaccattgcctatttggtccttggataaagctaatgttttggcctcttcaagattatggagaaaaactgtggaggaaaccatcaagaacgaatttcctacattgaaggttcaacatcaattgattgattctgccgccatgatcctagttaagaacccaacccacctaaatactcggtggtaacggataaaccaggaacctatttcgattacaaaaccggagaagttctaatacctctttttgacacctcctttggtagttcttgcttaaaggatgtaacttccaagttgtagttaactaactaagacggcggtactaggatcaattcttgggttgggtggatttaLEU219,26019,28019,30019,32019,34019,36019,38019,40019,420
    ggtattataatcaccagcaacatgtttggtgatatcatctccgatgaagcctccgttatcccaggttccttgggtttgttgccatctgcgtccttggcctctttgccagacaagaacaccgcatttggtttgtacgaaccatgccacggttctgctccagatttgccaaagaataccataatattagtggtcgttgtacaaaccactatagtagaggctacttcggaggcaatagggtccaaggaacccaaacaacggtagacgcaggaaccggagaaacggtctgttcttgtggcgtaaaccaaacatgcttggtacggtgccaagacgaggtctaaacggtttcttatLEU219,44019,46019,48019,50019,52019,54019,56019,58019,600
    aggtcaaccctatcgccactatcttgtctgctgcaatgatgttgaaattgtcattgaacttgcctgaagaaggtaaggccattgaagatgcagttaaaaaggttttggatgcaggtatcagaactggtgatttaggtggttccaacagtaccaccgaagtcggtgatgctgtcgctccagttgggatagcggtgatagaacagacgacgttactacaactttaacagtaacttgaacggacttcttccattccggtaacttctacgtcaatttttccaaaacctacgtccatagtcttgaccactaaatccaccaaggttgtcatggtggcttcagccactacgacagcgLEU219,62019,64019,66019,68019,70019,72019,74019,760
    cgaagaagttaagaaaatccttgcttaaaaagattctctttttttatgatatttgtacataaactttataaatgaaattcataatagaaacgacacgaaattacaaaatggaatatgttcatagggtagacgaaactatatacgcaatctacatacatttatcaagaaggagaaagcttcttcaattcttttaggaacgaatttttctaagagaaaaaaatactataaacatgtatttgaaatatttactttaagtattatctttgctgtgctttaatgttttaccttatacaagtatcccatctgctttgatatatgcgttagatgtatgtaaatagttcttcctctttLEU2 TerminatorLEU219,78019,80019,82019,84019,86019,88019,90019,92019,940
    aaggaggatgtaaaggaatacaggtaagcaaattgatactaatggctcaacgtgataaggaaaaagaattgcactttaacattaatattgacaaggaggagggcaccacacaaaaagttaggtgtaacagaaaatcatgaaactatgattcctaatttatatattggaggattttttcctcctacatttccttatgtccattcgtttaactatgattaccgagttgcactattcctttttcttaacgtgaaattgtaattataactgttcctcctcccgtggtgtgtttttcaatccacattgtcttttagtactttgatactaaggattaaatatataacctcctaaaaLEU2 Terminator19,96019,98020,00020,02020,04020,06020,08020,10020,120
    ctctaaaaaaaaaaaaatacaacaaataaaaaacactcaatgacctgaccatttgatggagtttaagtcaataccttcttgaaccatttcccataatggtgaaagttccctcaagaattttactctgtcagaaacggccttaacgacgtagtcgatgacggatcgcttgcctgtagagatttttttttttttatgttgtttattttttgtgagttactggactggtaaactacctcaaattcagttatggaagaacttggtaaagggtattaccactttcaagggagttcttaaaatgagacagtctttgccggaattgctgcatcagctactgcctagcgaacggacatLEU2 TerminatorARS420,14020,16020,18020,20020,22020,24020,26020,28020,300
    acttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgtttatttatttttatgttttgtatttggattttagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaataaataaaaatacaaaacataaacctaaaatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatgARS420,32020,34020,36020,38020,40020,42020,44020,460
    tttacatatatatttattagacaagaaaagcagattaaatagatatacattcgattaacgataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgctattcattttacattttagtgtcctaaaagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctcgttctattttccatcataaacARS420,48020,50020,52020,54020,56020,58020,60020,62020,640
    ttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaatttctttttttactttctatttttaatttatatatttatattaaaaaatttaaattataattatttttatagcacgtcatctgcgagctcGATCCTTTGATCTTTTCTACGGGGTCaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaaagaaaaaaatgaaagataaaaattaaatatataaatataattttttaaatttaatattaataaaaatatcgtgcagtagacgctcgagCTAGGAAACTAGAAAAGATGCCCCAGSwaICen6ARS420,66020,68020,70020,72020,74020,76020,78020,80020,820
    tgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcattctaggtactaaaacaattcatccagtaaaatataatattttattttctcccaatcaggcttgatccccagtaagtcaaaaaatagctcgacatactgttcttccccgatatcctccctgatcgacactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtacgtaagatccatgattttgttaagtaggtcattttatattataaaataaaagagggttagtccgaactaggggtcattcagttttttatcgagctgtatgacaagaaggggctataggagggactagctgaminoglycoside phosphotransferase20,84020,86020,88020,90020,92020,94020,96020,98021,000
    cggacgcagaaggcaatgtcataccacttgtccgccctgccgcttctcccaagatcaataaagccacttactttgccatctttcacaaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttcctcttcgggcttttccgtctttaaaaaatcatacagctcgcgcggcctgcgtcttccgttacagtatggtgaacaggcgggacggcgaagagggttctagttatttcggtgaatgaaacggtagaaagtgtttctacaacgacagagggtccagcggcacccttttctgttcaaggagaagcccgaaaaggcagaaattttttagtatgtcgagcgcgcaminoglycoside phosphotransferase21,02021,04021,06021,08021,10021,12021,14021,160
    gatctttaaatggagtgtcttcttcccagttttcgcaatccacatcggccagatcgttattcagtaagtaatccaattcggctaagcggctgtctaagctattcgtatagggacaatccgatatgtcgatggagtgaaagagcctgatgcactccgcatacagctcgataatcttctagaaatttacctcacagaagaagggtcaaaagcgttaggtgtagccggtctagcaataagtcattcattaggttaagccgattcgccgacagattcgataagcatatccctgttaggctatacagctacctcactttctcggactacgtgaggcgtatgtcgagctattagaaBlpIaminoglycoside phosphotransferase21,18021,20021,22021,24021,26021,28021,30021,32021,340
    ttcagggctttgttcatcttcatactcttccgagcaaaggacgccatcggcctcactcatgagcagattgctccagccatcatgccgttcaaagtgcaggacctttggaacaggcagctttccttccagccatagcatcatgtccttttcccgttccacatcataggtggtccctaagtcccgaaacaagtagaagtatgagaaggctcgtttcctgcggtagccggagtgagtactcgtctaacgaggtcggtagtacggcaagtttcacgtcctggaaaccttgtccgtcgaaaggaaggtcggtatcgtagtacaggaaaagggcaaggtgtagtatccaccagggaaminoglycoside phosphotransferase21,36021,38021,40021,42021,44021,46021,48021,50021,520
    ttataccggctgtccgtcatttttaaatataggttttcattttctcccaccagcttatataccttagcaggagacattccttccgtatcttttacgcagcggtatttttcgatcagttttttcaattccggtgatattctcattttagccatttattatttccttcctcttttctaatatggccgacaggcagtaaaaatttatatccaaaagtaaaagagggtggtcgaatatatggaatcgtcctctgtaaggaaggcatagaaaatgcgtcgccataaaaagctagtcaaaaaagttaaggccactataagagtaaaatcggtaaataataaaggaaggagaaaagaaminoglycoside phosphotransferase21,54021,56021,58021,60021,62021,64021,66021,68021,700
    acagtatttaaagataccccaagaagctaattataacaagacgaactccaattcactgttccttgcattctaaaaccttaaataccagaaaacagctttttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgattttgaaaccgcggtgatcacaggcatgtcataaatttctatggggttcttcgattaatattgttctgcttgaggttaagtgacaaggaacgtaagattttggaatttatggtcttttgtcgaaaaagtttcaacaaaagtttcaaccgcatattgtatcatagctgcctcggctaaaactttggcgccactagtgtccgtSacII21,72021,74021,76021,78021,80021,82021,84021,860
    gcaacgctctgtcatcgttacaatcaacatgctaccctccgcgagatcatccgtgtttcaaacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtatccgttgcgagacagtagcaatgttagttgtacgatgggaggcgctctagtaggcacaaagtttgggccgtcgaatcaacggcaagaaggcttatcgtagccattgtactcgtttcagacggcggaatgttgccgagagggcgactgcggcagggcctgactacccgacggacatag21,88021,90021,92021,94021,96021,98022,00022,02022,040
    gagtggtgattttgtgccgagctgccggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgcttagacaacttaataacacattgcggacgtttttaatgtactgaattaacgccgaattaattcgggggatctggattttagtactggatctcaccactaaaacacggctcgacggccagcccctcgacaaccgaccgaccaccgtcctatataacaccacatttgtttaactgcgaatctgttgaattattgtgtaacgcctgcaaaaattacatgacttaattgcggcttaattaagccccctagacctaaaatcatgacctaCaMV 3'UTR (polyA signal)left borde...C58 T-DNA22,06022,08022,10022,12022,14022,16022,18022,20022,220
    tttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctaattcccttatctgggaactactcacacattattatggagaaactcgagcttgtcgatcgactctaaaccaaaatccttaatctttaaaataactatcttcataaaatgtttatgtttatgtatgattcccaaagaatatacgagttgtgtactcgctttgggatatccttgggattaagggaatagacccttgatgagtgtgtaataatacctctttgagctcgaacagctagctgagaPspXICaMV 3'UTR (polyA signal)22,24022,26022,28022,30022,32022,34022,36022,38022,400
    agctagaggatcgatccgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgtcgatctcctagctaggcttggggtctcagggcgagtcttcttgagcagttcttccgctatcttccgctacgcgacgcttagccctcgccgctatggcatttcgtgctccttcgccagtcgggtaagcggcggttcgagaagtcgttatagtgcccatcggttgcgatacaggacneomycin phosphotransferase22,42022,44022,46022,48022,50022,52022,54022,560
    atagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgtgtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttatcgccaggcggtgtgggtcggccggtgtcagctacttaggtcttttcgccggtaaaaggtggtactataagccgttcgtccgtagcggtacacagtgctgctctaggagcggcagcccgtacgcgcggaactcggaccgcttgtcaagccgaccgcgctcggggactacgagaRsrIIneomycin phosphotransferase22,58022,60022,62022,64022,66022,68022,70022,72022,740
    tcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggaagcaggtctagtaggactagctgttctggccgaaggtaggctcatgcacgagcgagctacgctacaaagcgaaccaccagcttacccgtccatcggcctagttcgcatacgtcggcggcgtaacgtagtcggtactacctatgaaagagccgtcctcgttccactctactgtcctneomycin phosphotransferase22,76022,78022,80022,82022,84022,86022,88022,90022,920
    gatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctggagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgctaggacggggccgtgaagcgggttatcgtcggtcagggaagggcgaagtcactgttgcagctcgtgtcgacgcgttccttgcgggcagcaccggtcggtgctatcggcgcgacggagcaggacctcaagtaagtcccgtggcctgtccagccagaactgtttttcttggcccgcFspIneomycin phosphotransferase22,94022,96022,98023,00023,02023,04023,06023,08023,100
    cccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatccccatggtcgatcgacagatctgcgaaagctcgagagagatagatttgggggacgcgactgtcggccttgtgccgccgtagtctcgtcggctaacagacaacacgggtcagtatcggcttatcggagaggtgggttcgccggcctcttggacgcacgttaggtagaacaagttaggggtaccagctagctgtctagacgctttcgagctctctctatctaaacNcoIneomycin phosphotransferaseCaM...ed23,12023,14023,16023,18023,20023,22023,24023,260
    tagagagagactggtgatttcagcgtgtcctctccaaatgaaatgaacttccttatatagaggaaggtcttgcgaaggatagtgggattgtgcgtcatcccttacgtcagtggagatatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgatctctctctgaccactaaagtcgcacaggagaggtttactttacttgaaggaatatatctccttccagaacgcttcctatcaccctaacacgcagtagggaatgcagtcacctctatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacCaMV35S2; CaMV 35S promoter, duplicated23,28023,30023,32023,34023,36023,38023,40023,42023,440
    ctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattagaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttgctatcggaaaggaaatagcgttactaccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataatPshAICaMV35S2; CaMV 35S promoter, duplicated23,46023,48023,50023,52023,54023,56023,58023,60023,620
    ccctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgctccaccatgttatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcctcgtgggtgggggtccatcgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactataagaacctcatctgctctcacagcacgaggtggtacaatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacgaggagcacccacccccaggtagCaMV35S2; CaMV 35S promoter, duplicated23,64023,66023,68023,70023,72023,74023,76023,78023,800
    tttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattaccctttgttgaaaagtctcaataaaaccctggtgacagccgtctccgtagaacttgctatcggaaaggaaatagcgttactaccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataatgggaaacaacttttcagagttatPshAICaMV35S2; CaMV 35S promoter, duplicated23,82023,84023,86023,88023,90023,92023,94023,960
    gccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgctccaccatgttggcaagctgctctagccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgacgggaaaccagaagactctgacatagaaactataagaacctcatctgctctcacagcacgaggtggtacaaccgttcgacgagatcggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactCaMV35S2; CaMV 35S promoter, duplicated23,98024,00024,02024,04024,06024,08024,10024,12024,140
    gcgcaacgcaattaatGTGAGTTAGCTCACTCATTAGGCAactagacgcgttgcgttaattaCACTCAATCGAGTGAGTAATCCGTtgatct24,16024,180
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    24196 bp17500200002250025005000UBQ10 promoterUBQ10f primer binding2249-39012210-2212Cas9_split-f (DNA)TPLRD2-split-r (DNA)RareCut-MluIAscISbfI,tOCS,pUBQ1,SbfI,AscI,BlpI,PspOMI,ApaI,MreI,TspMI,XmaI,SmaIpUBQ1_gRNA_Target1,PAM,SV40NLS,GS12b,VENUS-N7,first diff from YFP,StuIGS12cpr268luc+ geneSTOP,PaqCItUBQ1SalI,HindIIIU6 promoter paperpUBQ1_gRNA_Target1,gRNA Handle,U6 terminator paper,+2Acc65IKpnINruIHpaILEU2 PromoterLEU2LEU2 TerminatorARS4Cen6,Cen6,SwaIBlpIaminoglycoside phosphotransferaseleft border repeat from C58 T-DNA,CaMV 3'UTR (polyA signal),SacIIneomycin phosphotransferase,PspXI,RsrII,FspICaMV35S2; CaMV 35S promoter, duplicated,NcoI,PshAI,PshAI

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    24196