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  • 0 feature results
pFLAG-SNAP-MCS-HIStag
  • Sequence Map
    Editing disabled on read-only sequence.
    TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAM13 originF1 ori20406080100120140160
    TTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAAloIBsaAIBsaXIDraIIIM13 originF1 ori180200220240260280300320340
    CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTAPsiISspIM13 origin360380400420440460480500520
    ACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATGTAAGTTTATACATAGGCGAGTACTTAATTAAGAATCTTTTTGAGTAGCTCGTAGTTTACTTTGACGTTAAATAAGTATAGTCCTAATAGTTATGGTATAAAAACTTTTTCGGCAAAGACATTACTTCCTCTTTTGAGTGGCTCCGTCAAGGTATCCTACCGTTCTAGGACCATKanR540560580600620640660680700
    TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCAAGCCAGACGCTAAGGCTGAGCAGGTTGTAGTTATGTTGGATAATTAAAGGGGAGCAGTTTTTATTCCAATAGTTCACTCTTTAGTGGTACTCACTGCTGACTTAGGCCACTCTTACCGTTTTCAAATACGTAAAGAAAGGTCTGAACAAGTTGTCCGGTCGGTAATGCGAGCAGTNsiIKanR720740760780800820840860
    TCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATAAGTTTTAGTGAGCGTAGTTGGTTTGGCAATAAGTAAGCACTAACGCGGACTCGCTCTGCTTTATGCGCTAGCGACAATTTTCCTGTTAATGTTTGTCCTTAGCTTACGTTGGCCGCGTCCTTGTGACGGTCGCGTAGTTGTTATAAAAGTGGACTTAGTCCTATAAGAAGATTATAjuIBsmIPvuISspIAsiSIEcoNIBpu10IKanR8809009209409609801,0001,0201,040
    CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCGGACCTTACGACAAAAGGGCCCCTAGCGTCACCACTCATTGGTACGTAGTAGTCCTCATGCCTATTTTACGAACTACCAGCCTTCTCCGTATTTAAGGCAGTCGGTCAAATCAGACTGGTAGAGTAGACATTGTAGTAACCGTTGCGATGGAAACGGTACAAAGTCTTTGTTGAGAvaIBsmINsiISmaIXmaIBsoBITspMIKanR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGACCGCGTAGCCCGAAGGGTATGTTAGCTATCTAACAGCGTGGACTAACGGGCTGTAATAGCGCTCGGGTAAATATGGGTATATTTAGTCGTAGGTACAACCTTAAATTAGCGCCGGATCTCGTTCTGCAAAGGGCAACTTATACCGAGTATTGTGGGGAACATAATGACAAATACClaINruIBspDIKanR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGATTCGTCTGTCAAAATAACAAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCColE1 origin1,4201,4401,4601,4801,5001,5201,5401,560
    GATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATACTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATAcuIColE1 origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGColE1 origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCBssSIBssSαIColE1 origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGACGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTPciISapIBspQI2,1202,1402,1602,1802,2002,2202,2402,260
    TGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGAccITatIBsaAIPflFIBstZ17ITth111I2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGPfoI2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACAACTCAAAGAGGTCTTCGCAATTACAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTT2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGGTACGGGCCAATGACCTTGCAACACTCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGC2,8202,8402,8602,8802,9002,9202,9402,960
    CTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGACTGAAGGCGCAAAGGTCTGAAATGCTTTGTGCCTTTGGCTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCPpuMIBpu10I2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGCTCGTGCTAGTACGCGTGGGCACCCCGGCGGTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCBglIFspIFspAI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTTTTACTGGGTCTCGCGACGGCCGTGGACAGGATGCTCAACGTACTATTTCTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAPshAI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCBsaXI3,5203,5403,5603,5803,6003,6203,6403,660
    AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCATAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGHpaIBsrDIEcoRVBssHIIHincII3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCApaIBstEIIPspOMINmeAIII4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGBclIMluIBsrDI4,2204,2404,2604,2804,3004,3204,3404,360
    GTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAACACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATT4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGPfoIEcoNIBstAPI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTACCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCSphI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAGCGGTCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCTCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGGTACCTBarINcoIStyIXbaIBglIISgrAIMD2LacOT74,9204,9404,9604,9805,0005,0205,0405,060
    CTACAAGGATGACGATGACAAGCTTACCGGTGAGTTCGCTATGGACAAAGATTGCGAAATGAAACGTACCACCCTGGATAGCCCGCTGGGCAAACTGGAACTGAGCGGCTGCGAACAGGGCCTGCATGAAATTAAACTGCTGGGTAAAGGCACCAGCGCGGCCGATGCGGTTGAAGATGTTCCTACTGCTACTGTTCGAATGGCCACTCAAGCGATACCTGTTTCTAACGCTTTACTTTGCATGGTGGGACCTATCGGGCGACCCGTTTGACCTTGACTCGCCGACGCTTGTCCCGGACGTACTTTAATTTGACGACCCATTTCCGTGGTCGCGCCGGCTACGCCAACTTAgeIBglIHindIIIYK4DD6DD8KL10TG12EF14AM16DK18DC20EM22KR24TT26LD28SP30LG32KL34EL36SG38CE40QG42LH44EI46KL48LG50KG52TS54AA56DA58VE605071-5733Expressed CDSSNAP26b CDSGibson rvGibson fwFLAG Tagmulti...oRI)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GTTCCGGCCCCGGCCGCCGTGCTGGGTGGTCCGGAACCGCTGATGCAGGCGACCGCGTGGCTGAACGCGTATTTTCATCAGCCGGAAGCGATTGAAGAATTTCCGGTTCCGGCGCTGCATCATCCGGTGTTTCAGCAGGAGAGCTTTACCCGTCAGGTGCTGTGGAAACTGCTGACAAGGCCGGGGCCGGCGGCACGACCCACCAGGCCTTGGCGACTACGTCCGCTGGCGCACCGACTTGCGCATAAAAGTAGTCGGCCTTCGCTAACTTCTTAAAGGCCAAGGCCGCGACGTAGTAGGCCACAAAGTCGTCCTCTCGAAATGGGCAGTCCACGACACCTTTGACGACTMluIVP62AP64AA66VL68GG70PE72PL74MQ76AT78AW80LN82AY84FH86QP88EA90IE92EF94PV96PA98LH100HP102VF104QQ106ES108FT110RQ112VL114WK116LL1185071-5733Expressed CDSSNAP26b CDS5,2605,2805,3005,3205,3405,3605,3805,4005,420
    AAGTGGTTAAATTTGGCGAAGTGATTAGCTATCAGCAGCTGGCGGCCCTGGCGGGTAATCCGGCGGCCACCGCCGCCGTTAAAACCGCGCTGAGCGGTAACCCGGTGCCGATTCTGATTCCGTGCCATCGTGTGGTTAGCTCTAGCGGTGCGGTTGGCGGTTATGAAGGTGGTCTTTCACCAATTTAAACCGCTTCACTAATCGATAGTCGTCGACCGCCGGGACCGCCCATTAGGCCGCCGGTGGCGGCGGCAATTTTGGCGCGACTCGCCATTGGGCCACGGCTAAGACTAAGGCACGGTAGCACACCAATCGAGATCGCCACGCCAACCGCCAATACTTCCACCAGABlpIBstEIIKV120VK122FG124EV126IS128YQ130QL132AA134LA136GN138PA140AT142AA144VK146TA148LS150GN152PV154PI156LI158PC160HR162VV164SS166SG168AV170GG172YE174GG176L5071-5733Expressed CDSSNAP26b CDS5,4405,4605,4805,5005,5205,5405,5605,5805,600
    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*5071-5733Expressed CDSSNAP26b CDSMCSGibson2 fwGibson2 rv6x His Tag5,6205,6405,6605,6805,7005,7205,7405,760
    GCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATCGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTABlpIStyIrv...g5,7805,8005,8205,8405,860
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5869 bp42504500475050005250550025050075010001250ColE1 originKanRF1 oriMCSGibson2 rv5071-5733

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    5869