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URA3 locus (BY4741) for YTK integration using CRISPR
  • Sequence Map
    Editing disabled on read-only sequence.
    GGGCGGATTACTACCGTTGCTAAATTCGAGTGAAACACAGGAAGAtCAGAAAATCCTCATTTCATCCATATTAACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGcAAGACAAGCAACGAAACGTTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACCCCGCCTAATGATGGCAACGATTTAAGCTCACTTTGTGTCCTTCTaGTCTTTTAGGAGTAAAGTAGGTATAATTGTTATTAAAGTTTACAAATAAACGTAATAAACTTTGATCCgTTCTGTTCGTTGCTTTGCAAAAACTTTTAAAACTCATAAAAGTTATTTAAACATCTCCTGAciIAclIBfaIDpnIEciIFokIMboIMwoITaqIBfuCIBtsCIDpnIITaqαISau3AIYTK's URA3 5' homologyURA3 5... site20406080100120140160
    TCAGATATTGAAAAAAAGCTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCATGGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCAATTGATGACAATACAGACGATGATAACAAACCGAAGTAGTCTATAACTTTTTTTCGATGTCGTTAATTATGAACTATTCTTCTCATAACTCTTCCCGTTGCCAAGTAGTAGAGTACCTAGACGTGTACTTGTTTGTGGTCTCAGTTTGCTGCAACTTTAACTCCGATGACGCGGTTAACTACTGTTATGTCTGCTACTATTGTTTGGCTTCAAlwIAseIBsgIDpnIEarIHhaIMboIMfeISfcIBfuCIBstYIDpnIIHinP1IHpy99ISau3AIYTK's URA3 5' homology180200220240260280300320340
    TATCTGATGTAGAAAAGGATTAAAGATGCTAAGAGATAGTGATGATATTTCATAAATAATGTAATTCTATATATGTTAATTACCTTTTTTGCGAGGCATATTTATGGTGAAGGATAAGTTTTGACCATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATAAAgctcccgATAGACTACATCTTTTCCTAATTTCTACGATTCTCTATCACTACTATAAAGTATTTATTACATTAAGATATATACAATTAATGGAAAAAACGCTCCGTATAAATACCACTTCCTATTCAAAACTGGTAGTTTCTTCCAATTACACCGACACCAAAGTCCCAGGTATTTcgagggcAvaIHphIMslIMspISmaIXmaIAvaIIBsoBIHpaIISfaNITspMIYTK's URA3 5' homologygRNA - pRC251360380400420440460480500520
    ggaatctcggtcgtaatgatttctataatgacgaaaaaaaaaaaattggaaagaaaaagcttcatggcctttataaaaaggaactatccaatacctcgccagaaccaagtaacagtattttacggggcacaaatcaagaacaataagacaggactgtaaagatggacgcattgaaccttagagccagcattactaaagatattactgcttttttttttttaacctttctttttcgaagtaccggaaatatttttccttgataggttatggagcggtcttggttcattgtcataaaatgccccgtgtttagttcttgttattctgtcctgacatttctacctgcgtaacttHgaIPsiITfiIBaeGIBsiEIHindIIIBsp1286IYTK's URA3 3' homology540560580600620640660680700
    ctccaaagaacaacaagagttccaaaaagtagtggaacaaaagcaaatgaaggatttcatgcgtttgtactctaatctggtagaaagatgtttcacagactgtgtcaatgacttcacaacatcaaagctaaccaataaggaacaaacatgcatcatgaagtgctcagaaaagttcgaggtttcttgttgttctcaaggtttttcatcaccttgttttcgtttacttcctaaagtacgcaaacatgagattagaccatctttctacaaagtgtctgacacagttactgaagtgttgtagtttcgattggttattccttgtttgtacgtagtacttcacgagtcttttcaagNsiINspIRsaITatIXmnIBspHICviQIPflFISfaNIBsiHKAITth111IBsp1286IYTK's URA3 3' homology720740760780800820840860
    ttgaagcatagcgaacgtgtagggcagcgtttccaagaacaaaacgctgccttgggacaaggcttgggccgataaggtgtactggcgtatatatatctaattatgtatctctggtgtagcccatttttagcatgtaaatataaagagaaaccatatctaatctaaccaaatccaaaacttcgtatcgcttgcacatcccgtcgcaaaggttcttgttttgcgacggaaccctgttccgaacccggctattccacatgaccgcatatatatagattaatacatagagaccacatcgggtaaaaatcgtacatttatatttctctttggtatagattagattggtttaggttNspIRsaIStyITatIBsmFICviQIAflIIIHpy166IIYTK's URA3 3' homologyURA3 3... site8809009209409609801,0001,0201,040
    acaaaattcaatagttactatcgcttttttctttctgtatcgcaaataagtgaaaattaaaaaagaaagattaaattggaagttggatatgggctggaacagcagcagtaatcggtatcgggttcgccactaatgacgtcctacgattgcactcaacagaccttgacgctcacgctgttttaagttatcaatgatagcgaaaaaagaaagacatagcgtttattcacttttaattttttctttctaatttaaccttcaacctatacccgaccttgtcgtcgtcattagccatagcccaagcggtgattactgcaggatgctaacgtgagttgtctggaactgcgagtgcgFauIHgaIMmeIMwoIZraIAatIIBceAIBsaHIEcoP15IEcoP15I1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgtagcgggcgacaagtcaaacggaacaaccgttgccgttcccatcggagtccgacctaggccgaactccgtgaatttctgataacaacggtcggtaaagactggttccccagtatatttcttctctcaggagcaggggccaatgccaaaagcgacattaacccggaggacaagggcatcgcccgctgttcagtttgccttgttggcaacggcaagggtagcctcaggctggatccggcttgaggcacttaaagactattgttgccagccatttctgaccaaggggtcatataaagaagagagtcctcgtccccggttacggttttcgctgtaattgggcctcctgttccAciIBfaIBmrIMmeIMspIStyIAvrIIBceAIBsiEICac8IHpaII1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ctccactgtgttccaccgaatttcccacctgataatatctgataacgaggtgacacaaggtggcttaaagggtggactattatagactattgTscAITspRIBtsIMutI1,4201,440
    • Linear Map
    • Relevant Items
    • Metadata
    • Description
    Editing disabled on read-only sequence.
    URA3 locus (BY4741) for YTK integration using CRISPR (1446 bp)AciIAciIAclIAlwIAseIAvaIBfaIBfaIBmrIBsgIDpnIDpnIEarIEciIFauIFokIHgaIHgaIHhaIHphIMboIMboIMfeIMmeIMmeIMslIMspIMspIMwoIMwoINsiINspINspIPsiIRsaIRsaISfcISmaIStyIStyITaqITatITatITfiIXmaIXmnIZraIAatIIAvaIIAvrIIBaeGIBceAIBceAIBfuCIBfuCIBsaHIBsiEIBsiEIBsmFIBsoBIBspHIBstYIBtsCICac8ICviQICviQIDpnIIDpnIIHpaIIHpaIIPflFISfaNISfaNITaqαITscAITspMITspRIAflIIIHinP1IHpy99ISau3AISau3AIBsiHKAIEcoP15IEcoP15IHindIIITth111IBsp1286IBsp1286IBtsIMutIHpy166II2004006008001,0001,2001,400YTK's URA3 5' homologyYTK's URA3 3' homologyURA3 locus (BY4741) for YTK integration using CRISPR (1446 bp)

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    1446