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Nelf-b-dTAG-HA-T2A-Puro
  • Sequence Map
    Editing disabled on read-only sequence.
    tctgctcacccgggtcttgttcagaatttgggacaaagagagtcccttgagggcaggggccgagccaccctgttctgtttacagtggaaacgtttggtgacctggccttcagtgacatctttctccacctgctcactgggagcctggtactgcttgctgatgaatttgccctggaagacgagtgggcccagaacaagtcttaaaccctgtttctctcagggaactcccgtccccggctcggtgggacaagacaaatgtcacctttgcaaaccactggaccggaagtcactgtagaaagaggtggacgagtgaccctcggaccatgacgaacgactacttaaacgggacctSmaIXmaITspMILeft arm20406080100120140160
    ggatttctgcagcagcctctttgatggcttcttcctcactgcctctcccaggtaggacacgcacatttcctgccagttcttatgtttgacagcttgggaggagagttgaggccccatcctgctaaaagaccacaggtgggtctctgagcactgagacctgtgctggactgaggaacctaaagacgtcgtcggagaaactaccgaagaaggagtgacggagagggtccatcctgtgcgtgtaaaggacggtcaagaatacaaactgtcgaaccctcctctcaactccggggtaggacgattttctggtgtccacccagagactcgtgactctggacacgacctgactccttEcoRILeft arm180200220240260280300320340
    ttcctgcatggagcttgggggtgaccagggctcagtgctgatccacctctaggtggagtggggtaagctgaagctcatgctgaccccagggtgggttgtgcctaggagtgaagcaggtgtcacggcacctccagctccactgatctcttccaggaaggagaatgtgcaccgtcataaggacgtacctcgaacccccactggtcccgagtcacgactaggtggagatccacctcaccccattcgacttcgagtacgactggggtcccacccaacacggatcctcacttcgtccacagtgccgtggaggtcgaggtgactagagaaggtccttcctcttacacgtggcagtaPasIAvrIILeft arm360380400420440460480500520
    gtgctgaggcttctcctccacttgcatgcaagagtggccccttccaagctggaggcattgcagaaggctctggagcccacaggccaggtgggtatcatcagttcctacttagagggcattgttctgtgaatgtgcttggccagtccagctgtggcatgatgcccttggcgcttgccacgactccgaagaggaggtgaacgtacgttctcaccggggaaggttcgacctccgtaacgtcttccgagacctcgggtgtccggtccacccatagtagtcaaggatgaatctcccgtaacaagacacttacacgaaccggtcaggtcgacaccgtactacgggaaccgcgaacgMscIPsrISphIBbvCIBpu10ILeft arm540560580600620640660680700
    tttgatggtgtttttcatgattggtcttgcctctgggttcactcctccatgtaggctcagacagaccccctgtctatggcatgccatgtctggttttcctacagagcggggaggccgtgaaggagctctactcccagcttggtgagaagttggagcagttagaccatcgaaagccaaactaccacaaaaagtactaaccagaacggagacccaagtgaggaggtacatccgagtctgtctgggggacagataccgtacggtacagaccaaaaggatgtctcgcccctccggcacttcctcgagatgagggtcgaaccactcttcaacctcgtcaatctggtagctttcggAhdIBarIBplIBplISphILeft arm720740760780800820840860
    cagtcctacccaagctgcagagacaccagcactggatctgcctcttcccagcgtgcctgctccagccggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgcttgaagatggaaagaaagttgtcaggatgggttcgacgtctctgtggtcgtgacctagacggagaagggtcgcacggacgaggtcggcctcacgtccacctttggtagaggggtcctctgcccgcgtggaaggggttcgcgccggtctggacgcaccacgtgatgtggccctacgaacttctacctttctttcaaBsgIConf by seqFKBP12Insert: dTAG-2xHA-P2A-PuroLeft arm8809009209409609801,0001,0201,040
    gattcctcccgggacagaaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgctaaggagggccctgtctttgttcgggaaattcaaatacgatccgttcgtcctccactaggctccgacccttcttccccaacgggtctactcacacccagtctctcggtttgactgatatagaggtctaatacggataccacggtgacccgtgggtccgtagtagggtggtgtacSmaIXmaITspMIConf by seqFKBP12Insert: dTAG-2xHA-P2A-Puro1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ccactctcgtcttcgatgtggagcttctaaaactggaaggctctggctacccctacgacgtgcccgactacgccggctatccgtatgatgtcccggactatgcaggcgctactaacttcagcctgctgaagcaggctggagacgtggaggagaaccctggacctatgaccgagtaggtgagagcagaagctacacctcgaagattttgaccttccgagaccgatggggatgctgcacgggctgatgcggccgataggcatactacagggcctgatacgtccgcgatgattgaagtcggacgacttcgtccgacctctgcacctcctcttgggacctggatactggctcatBbsINaeIBmgBIBmgBINgoMIVConf by seqInsert: dTAG-2xHA-P2A-Puro2x HAP2AFKBP12Puro1,2401,2601,2801,3001,3201,3401,3601,3801,400
    caagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcgttcgggtgccacgcggagcggtgggcgctgctgcaggggtcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgPasIZraIAatIIBsiWIBspEIPflFIRsrIITth111IConf by seqInsert: dTAG-2xHA-P2A-PuroPuro1,4201,4401,4601,4801,5001,5201,5401,560
    aaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgKasIKasINarINarISfoISfoIPluTIPluTISacIIConf by seqInsert: dTAG-2xHA-P2A-PuroPuro1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcacggcgcaccaaggaccggtggcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtgBcgIEagIMscIConf by seqInsert: dTAG-2xHA-P2A-PuroPuro1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacactgtgaggctgtggagaactgaaactgtttgacagtggtgccctatggaagggcacctagttggctaggggtgtaggctgagctgagaggggtgttctttggcaaggtgcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgttcgggccacggacttgtgacactccgacacctcttgactttgacaaactgtcaccacgggataccttcccgtggatcaaccgatccccacatccgactcgactctccccacaagaaaccgttccaBlpIZraIAatIISexAIBstAPIDraIIIConf by seqRight armInsert: dTAG-2xHA-P2A-PuroPuro1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tctccctcctctagtttctcccaggagctggtcccaaaaggtcctccctcagtggtctacttgcatatcatagggggcccttgatgtcatggtgggacttggcccctgcttgaccctggcagaggtggatggtgattgggatataggaaaagcagtgggcagaggcctgactgacagagggaggagatcaaagagggtcctcgaccagggttttccaggagggagtcaccagatgaacgtatagtatcccccgggaactacagtaccaccctgaaccggggacgaactgggaccgtctccacctaccactaaccctatatccttttcgtcacccgtctccggactgactgAccIApaIBsgIPflMIPpuMIPspOMIConf by seqRight arm2,1202,1402,1602,1802,2002,2202,2402,260
    ctgatattcaatctgcacaaagtccaaggtatgctaggttcaccaaagtagatttgctggacctgatccccaagcacaggagtggtaccctccacagaagctgggggagagtctgcagcaggcctgagcctgctgatgtcagtgctgactgagtccatgctaactgagatctgtagactataagttagacgtgtttcaggttccatacgatccaagtggtttcatctaaacgacctggactaggggttcgtgtcctcaccatgggaggtgtcttcgaccccctctcagacgtcgtccggactcggacgactacagtcacgactgactcaggtacgattgactctagacatAleIKpnIPsrIBglIIPflFIAcc65IBpu10ITth111IConf by seqRight arm2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggcagagctctgccctgtagctccctgcctcagtttatctttgcaatacaaagcaaatccttccccagctatgtgttttagcctttgagtaggggagtttcggtgtacacattgaattttccacttatgtaaataaaacggattttgataaatccatggtgaggaatctgtttgtccgtctcgagacgggacatcgagggacggagtcaaatagaaacgttatgtttcgtttaggaaggggtcgatacacaaaatcggaaactcatcccctcaaagccacatgtgtaacttaaaaggtgaatacatttattttgcctaaaactatttaggtaccactccttagacaaacaNcoIBsrGIConf by seqRight arm2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cctgggactgggttcagtggtggatgccatgaccaccttcaccacctcttccttctcttcctcctcttcctcctcctcttcgtttctttcttccttttttttttttttttttttttttttaatgttaggtctcactacctgtctccgactgtcctggaactcattgtataggctaggaccctgacccaagtcaccacctacggtactggtggaagtggtggagaaggaagagaaggaggagaaggaggaggagaagcaaagaaagaaggaaaaaaaaaaaaaaaaaaaaaaaaaattacaatccagagtgatggacagaggctgacaggaccttgagtaacatatccgatRight armConf by seq2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ggctatcctcgaacttggagagatcctgcctttgcctcccacatgctgggactagtgtgtgccaccacgcctggagggtgtaacattctggagagtcagtgctttggcctctgtgctgagtaacctggtgtagggcctggatccactagttctagagcggccgccaccgcggtggccgataggagcttgaacctctctaggacggaaacggagggtgtacgaccctgatcacacacggtggtgcggacctcccacattgtaagacctctcagtcacgaaaccggagacacgactcattggaccacatcccggacctaggtgatcaagatctcgccggcggtggcgccaccAleIEagINotISpeISpeIXbaIBamHIBstXIPflMISacIISexAIRight armBackBone-AmpR2,8202,8402,8602,8802,9002,9202,9402,960
    agctccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttcgaggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaPvuIBackBone-AmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgcgcagcctgaatggcgaatggaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagacgcgtcggacttaccgcttacctttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttcFspIPsiISspIBackBone-AmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    agtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcgAloIDrdIBsaAIBtgZIDraIIINgoMIVBackBone-AmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttgccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaaNaeIBackBone-AmpR3,5203,5403,5603,5803,6003,6203,6403,660
    atttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttSspIBciVIBackBone-AmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaaccattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgXmnIBssSIBssSαIBackBone-AmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaacagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattgBcgIPvuIScaIBackBone-AmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattagcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaatFspIBackBone-AmpR4,2204,2404,2604,2804,3004,3204,3404,360
    atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggatatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctAhdIBackBone-AmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaacagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttBackBone-AmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaagttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttcBackBone-AmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagaccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgBackBone-AmpR4,9204,9404,9604,9805,0005,0205,0405,060
    gatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccBciVIBackBone-AmpR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccDrdIBssSIBssSαIBackBone-AmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccgPciISapIBspQIAflIIIBackBone-AmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacaggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcBackBone-AmpR5,6205,6405,6605,6805,7005,7205,7405,760
    ctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccgggccccccctcgaggtcgacggtatcgatagatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgacccatggcccgggggggagctccagctgccatagctatAccIApaIClaIKpnISalITliIXhoIBspDIEcoRVPspXIAcc65IHincIIPaeR7IPspOMIBackBone-AmpR5,7805,8005,8205,8405,860
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    5870 bp42504500475050005250550025050075010001250Conf by seqPuroBackBone-AmpRTspMI,XmaI,SmaILeft arm,EcoRI,PasI,AvrII,BbvCI,Bpu10ISphI,PsrI,MscIAhdI,SphI,BarI,BplIBplIBsgI,TspMI,XmaI,SmaIFKBP12BbsI2x HA,P2A,BmgBI,NgoMIV,NaeI,BmgBIPflFIInsert: dTAG-2xHA-P2A-Puro,Tth111I,ZraI,AatII,PasI,BsiWIAhdIBciVI,BssSI,BssSαIDrdI,AflIII,PciIBspQI,SapI,Acc65I,KpnI,PspOMI,ApaI,PaeR7I,PspXI,TliI,XhoI,SalI,AccI,HincII,BspDI,+2

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    5870