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pE-SUMO-mH6-Cas13bt3
  • Sequence Map
    Editing disabled on read-only sequence.
    AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTCATCACCATCATCATCACGGGTCCCTGCAGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGATCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGGTACCCAGTAGTGGTAGTAGTAGTGCCCAGGGACGTCCTGAGTCTTCAGTTAGTTCTTCGATTCGGTCTKflINcoISbfIXbaIPpuMISUMO6xHis20406080100120140160
    GGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGACCAGTTCGGTCTTCAGTTCGGACTCTGAGTGTAGTTAAATTTCCACAGGCTACCTAGAAGTCTCTAGAAGAAGTTCTAGTTTTTCTGGTGAGGAAATTCTTCCGACTACCTTCGCAAGCGATTTTCTGTCCCATTCCTTTACCTGAGGAATTCTAAGAACATGCTGCCATAATCTDraIAflIIEcoRISUMO180200220240260280300320340
    ATTCAAGCTGATCAGGCCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACCGCGAACAGATTGGAGGTgaaaacttgtacttccaaggaggaggaATGGCCCAGGTGTCCAAGCAGACCAGCAAGAAGAGAGAGCTGAGCATCGACGAGTACCAGGGCGCCATAAGTTCGACTAGTCCGGGGACTTCTAAACCTGTACCTCCTATTGCTATAATAACTCCGAGTGGCGCTTGTCTAACCTCCActtttgaacatgaaggttcctcctcctTACCGGGTCCACAGGTTCGTCTGGTCGTTCTTCTCTCTCGACTCGTAGCTGCTCATGGTCCCGCGGTBseRIMA2QV4SK6QT8SK10KR12EL14SI16DE18YQ20GA22459-2786G1G2SUMOCas13b-t3Cas13b-t3-IF-fwpSUMO-IF-RvTEVIF360380400420440460480500520
    GAAAGTGGTGCTTCACAATCGCCTTCAACAAGGCCCTGGTCAACCGGGACAAGAACGACGGCCTGTTCGTGGAAAGCCTGCTGCGGCACGAGAAGTACAGCAAGCACGATTGGTACGACGAGGACACACGGGCCCTGATCAAGTGTTCTACCCAGGCCGCCAATGCCAAGGCCGACTTTCACCACGAAGTGTTAGCGGAAGTTGTTCCGGGACCAGTTGGCCCTGTTCTTGCTGCCGGACAAGCACCTTTCGGACGACGCCGTGCTCTTCATGTCGTTCGTGCTAACCATGCTGCTCCTGTGTGCCCGGGACTAGTTCACAAGATGGGTCCGGCGGTTACGGTTCCGGCTApaIHincIIPspOMIRK24WC26FT28IA30FN32KA34LV36NR38DK40ND42GL44FV46ES48LL50RH52EK54YS56KH58DW60YD62ED64TR66AL68IK70CS72TQ74AA76NA78KA80E459-2786Cas13b-t3540560580600620640660680700
    GGCTCTGAGAAACTACTTCAGCCACTACAGACACAGCCCCGGCTGCCTGACTTTTACCGCCGAGGATGAGCTGCGGACCATCATGGAAAGAGCCTACGAGCGGGCCATCTTCGAGTGCAGGCGGAGAGAAACCGAAGTGATCATCGAGTTCCCCAGCCTGTTCGAGGGCGACAGACCGAGACTCTTTGATGAAGTCGGTGATGTCTGTGTCGGGGCCGACGGACTGAAAATGGCGGCTCCTACTCGACGCCTGGTAGTACCTTTCTCGGATGCTCGCCCGGTAGAAGCTCACGTCCGCCTCTCTTTGGCTTCACTAGTAGCTCAAGGGGTCGGACAAGCTCCCGCTGTCTA82LR84NY86FS88HY90RH92SP94GC96LT98FT100AE102DE104LR106TI108ME110RA112YE114RA116IF118EC120RR122RE124TE126VI128IE130FP132SL134FE136GD138R459-2786Cas13b-t3HEPN1720740760780800820840860
    ATTACAACAGCCGGCGTGGTGTTCTTCGTCAGCTTCTTTGTGGAACGCAGAGTGCTGGACAGACTGTATGGCGCCGTGTCCGGCCTGAAGAAGAATGAGGGCCAGTACAAGCTGACCCGGAAGGCCCTGAGCATGTACTGCCTGAAGGACAGCAGATTCACCAAGGCCTGGGACATAATGTTGTCGGCCGCACCACAAGAAGCAGTCGAAGAAACACCTTGCGTCTCACGACCTGTCTGACATACCGCGGCACAGGCCGGACTTCTTCTTACTCCCGGTCATGTTCGACTGGGCCTTCCGGGACTCGTACATGACGGACTTCCTGTCGTCTAAGTGGTTCCGGACCCTGTI140TT142AG144VV146FF148VS150FF152VE154RR156VL158DR160LY162GA164VS166GL168KK170NE172GQ174YK176LT178RK180AL182SM184YC186LK188DS190RF192TK194AW196D459-2786Cas13b-t38809009209409609801,0001,0201,040
    AGCGGGTGCTGCTGTTCAGAGATATTCTGGCTCAGCTGGGCAGAATCCCCGCCGAGGCCTACGAATATTATCACGGCGAGCAGGGCGACAAGAAGCGGGCCAACGATAACGAGGGCACAAACCCCAAGCGGCACAAGGACAAGTTTATCGAGTTCGCCCTGCACTACCTGGAAGCTCGCCCACGACGACAAGTCTCTATAAGACCGAGTCGACCCGTCTTAGGGGCGGCTCCGGATGCTTATAATAGTGCCGCTCGTCCCGCTGTTCTTCGCCCGGTTGCTATTGCTCCCGTGTTTGGGGTTCGCCGTGTTCCTGTTCAAATAGCTCAAGCGGGACGTGATGGACCTTCGK198RV200LL202FR204DI206LA208QL210GR212IP214AE216AY218EY220YH222GE224QG226DK228KR230AN232DN234EG236TN238PK240RH242KD244KF246IE248FA250LH252YL254EA256459-2786Cas13b-t31,0601,0801,1001,1201,1401,1601,1801,2001,220
    CCAGCACAGCGAGATCTGCTTTGGCAGACGGCACATCGTGCGGGAAGAAGCTGGCGCTGGCGACGAGCACAAGAAGCACAGAACAAAGGGCAAAGTGGTGGTGGACTTCTCCAAGAAGGACGAGGACCAGAGCTACTACATCAGCAAGAACAACGTGATCGTGCGGATCGACAAGGGTCGTGTCGCTCTAGACGAAACCGTCTGCCGTGTAGCACGCCCTTCTTCGACCGCGACCGCTGCTCGTGTTCTTCGTGTCTTGTTTCCCGTTTCACCACCACCTGAAGAGGTTCTTCCTGCTCCTGGTCTCGATGATGTAGTCGTTCTTGTTGCACTAGCACGCCTAGCTGTTCQH258SE260IC262FG264RR266HI268VR270EE272AG274AG276DE278HK280KH282RT284KG286KV288VV290DF292SK294KD296ED298QS300YY302IS304KN306NV308IV310RI312DK314459-2786Cas13b-t31,2401,2601,2801,3001,3201,3401,3601,3801,400
    AATGCCGGACCTCGGAGCTACCGGATGGGCCTGAATGAGCTGAAGTACCTGGTCCTGCTGAGCCTGCAAGGCAAGGGCGACGATGCCATTGCCAAGCTGTACAGATACCGGCAGCACGTGGAAAACATCCTGGACGTGGTCAAAGTGACCGACAAGGATAACCATGTGTTTCTCCTTACGGCCTGGAGCCTCGATGGCCTACCCGGACTTACTCGACTTCATGGACCAGGACGACTCGGACGTTCCGTTCCCGCTGCTACGGTAACGGTTCGACATGTCTATGGCCGTCGTGCACCTTTTGTAGGACCTGCACCAGTTTCACTGGCTGTTCCTATTGGTACACAAAGAGGPmlIBmgBIBsrGISexAINA316GP318RS320YR322MG324LN326EL328KY330LV332LL334SL336QG338KG340DD342AI344AK346LY348RY350RQ352HV354EN356IL358DV360VK362VT364DK366DN368HV370FL372459-2786Cas13b-t31,4201,4401,4601,4801,5001,5201,5401,560
    CCAGATTCGTGCTGGAACAGCACGGCATCGGCAGAAAGGCCTTCAAGCAGAGAATCGACGGCAGAGTGAAGCACGTGCGCGGCGTGTGGGAGAAGAAAAAGGCCGCCACCAACGAGATGACCCTGCACGAGAAAGCCCGGGACATCCTGCAGTACGTGAACGAGAACTGCACCCGGGTCTAAGCACGACCTTGTCGTGCCGTAGCCGTCTTTCCGGAAGTTCGTCTCTTAGCTGCCGTCTCACTTCGTGCACGCGCCGCACACCCTCTTCTTTTTCCGGCGGTGGTTGCTCTACTGGGACGTGCTCTTTCGGGCCCTGTAGGACGTCATGCACTTGCTCTTGACGTGGGCPmlISmaIXmaITspMIPR374FV376LE378QH380GI382GR384KA386FK388QR390ID392GR394VK396HV398RG400VW402EK404KK406AA408TN410EM412TL414HE416KA418RD420IL422QY424VN426EN428CT430R459-2786Cas13b-t31,5801,6001,6201,6401,6601,6801,7001,7201,740
    CAGCTTCAACCCCGGCGAGTACAATAGACTGCTCGTGTGCCTCGTGGGCAAAGACGTCGAGAATTTCCAGGCCGGGCTGAAGAGACTGCAGCTGGCCGAGAGAATTGATGGCCGGGTGTACAGCATCTTCGCCCAGACCTCCACCATCAATGAGATGCACCAGGTCGTGTGCGACGTCGAAGTTGGGGCCGCTCATGTTATCTGACGAGCACACGGAGCACCCGTTTCTGCAGCTCTTAAAGGTCCGGCCCGACTTCTCTGACGTCGACCGGCTCTCTTAACTACCGGCCCACATGTCGTAGAAGCGGGTCTGGAGGTGGTAGTTACTCTACGTGGTCCAGCACACGCTGZraIAatIIBsrGISexAIS432FN434PG436EY438NR440LL442VC444LV446GK448DV450EN452FQ454AG456LK458RL460QL462AE464RI466DG468RV470YS472IF474AQ476TS478TI480NE482MH484QV486VC488D459-2786Cas13b-t31,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAGATCCTGAACCGGCTGTGCAGAATCGGCGACCAGAAACTGTACGACTACGTCGGCCTGGGCAAGAAGGATGAGATCGACTACAAGCAGAAGGTGGCCTGGTTCAAAGAGCACATCTCTATCAGACGGGGCTTCCTGCGGAAGAAGTTTTGGTACGATAGCAAGAAGGGCTTCGGTCTAGGACTTGGCCGACACGTCTTAGCCGCTGGTCTTTGACATGCTGATGCAGCCGGACCCGTTCTTCCTACTCTAGCTGATGTTCGTCTTCCACCGGACCAAGTTTCTCGTGTAGAGATAGTCTGCCCCGAAGGACGCCTTCTTCAAAACCATGCTATCGTTCTTCCCGAAGCBplIBplIQ490IL492NR494LC496RI498GD500QK502LY504DY506VG508LG510KK512DE514ID516YK518QK520VA522WF524KE526HI528SI530RR532GF534LR536KK538FW540YD542SK544KG546F459-2786Cas13b-t31,9401,9601,9802,0002,0202,0402,0602,0802,100
    CCAAGCTGGTCGAGGAACACCTGGAATCTGGCGGCGGACAGAGAGATGTGGGCCTCGATAAGAAGTACTACCACATCGACGCCATCGGAAGATTTGAGGGCGCCAATCCTGCTCTGTACGAGACACTGGCCAGAGACAGGCTGTGCCTGATGATGGCCCAGTACTTTCTGGGCAGGGTTCGACCAGCTCCTTGTGGACCTTAGACCGCCGCCTGTCTCTCTACACCCGGAGCTATTCTTCATGATGGTGTAGCTGCGGTAGCCTTCTAAACTCCCGCGGTTAGGACGAGACATGCTCTGTGACCGGTCTCTGTCCGACACGGACTACTACCGGGTCATGAAAGACCCGTCMscIA548KL550VE552EH554LE556SG558GG560QR562DV564GL566DK568KY570YH572ID574AI576GR578FE580GA582NP584AL586YE588TL590AR592DR594LC596LM598MA600QY602FL604GS606459-2786Cas13b-t32,1202,1402,1602,1802,2002,2202,2402,260
    CGTGCGCAAAGAGCTGGGCAACAAGATCGTGTGGTCCAACGACAGCATCGAGCTGCCTGTGGAAGGCTCCGTGGGCAACGAGAAATCCATCGTGTTCAGCGTGTCCGACTACGGCAAGCTGTATGTGCTGGACGACGCCGAGTTTCTGGGAAGAATCTGCGAGTACTTCATGCCCGCACGCGTTTCTCGACCCGTTGTTCTAGCACACCAGGTTGCTGTCGTAGCTCGACGGACACCTTCCGAGGCACCCGTTGCTCTTTAGGTAGCACAAGTCGCACAGGCTGATGCCGTTCGACATACACGACCTGCTGCGGCTCAAAGACCCTTCTTAGACGCTCATGAAGTACGGGFspIVR608KE610LG612NK614IV616WS618ND620SI622EL624PV626EG628SV630GN632EK634SI636VF638SV640SD642YG644KL646YV648LD650DA652EF654LG656RI658CE660YF662MP664459-2786V1E2G3S4V5G6N7E8K92334-2360Cas13b-t32,2802,3002,3202,3402,3602,3802,4002,4202,440
    CACGAGAAGGGCAAGATCAGATACCACACCGTGTACGAGAAAGGCTTCCGGGCCTACAACGACCTGCAGAAAAAATGCGTGGAAGCCGTGCTGGCCTTCGAGGAAAAAGTGGTCAAGGCCAAGAAGATGTCCGAGAAAGAGGGCGCCCACTACATCGACTTTCGCGAGATTCTGGGTGCTCTTCCCGTTCTAGTCTATGGTGTGGCACATGCTCTTTCCGAAGGCCCGGATGTTGCTGGACGTCTTTTTTACGCACCTTCGGCACGACCGGAAGCTCCTTTTTCACCAGTTCCGGTTCTTCTACAGGCTCTTTCTCCCGCGGGTGATGTAGCTGAAAGCGCTCTAAGACCNruIBfuAIBspMIHE666KG668KI670RY672HT674VY676EK678GF680RA682YN684DL686QK688KC690VE692AV694LA696FE698EK700VV702KA704KK706MS708EK710EG712AH714YI716DF718RE720IL722459-2786Cas13b-t32,4602,4802,5002,5202,5402,5602,5802,6002,620
    CCCAGACCATGTGCAAAGAGGCCGAGAAAACCGCCGTGAACAAAGTGCGGAGAGCCTTCTTTCACCACCACCTGAAGTTCGTGATCGATGAGTTCGGCCTGTTTAGCGACGTGATGAAGAAGTACGGCATCGAGAAAGAATGGAAGTTCCCCGTGAAGTGAactcgagcaCCACCGGGTCTGGTACACGTTTCTCCGGCTCTTTTGGCGGCACTTGTTTCACGCCTCTCGGAAGAAAGTGGTGGTGGACTTCAAGCACTAGCTACTCAAGCCGGACAAATCGCTGCACTACTTCTTCATGCCGTAGCTCTTTCTTACCTTCAAGGGGCACTTCACTtgagctcgtGGTGGClaITliIXhoIBmgBIBspDIPspXIPaeR7IAQ724TM726CK728EA730EK732TA734VN736KV738RR740AF742FH744HH746LK748FV750ID752EF754GL756FS758DV760MK762KY764GI766EK768EW770KF772PV774K*776459-2786Cas13b-t3IF-Cas13b-t3-ijns-RvIF-vec-fwHEPN2IF2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCTGGTGGTGGTGACTCTAGGCCGACGATTGTTTCGGGCTTTCCTTCGACTCAACCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTAACCGCTTACCCTGCGCGGGACATCGBspEI2,8202,8402,8602,8802,9002,9202,9402,960
    GGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATG2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCAloIPsiIBsaXI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTAC3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCATTAATTAAGAATCTTTTTGAGTAGCTCGTAGTTTACTTTGACGTTAAATAAGTATAGTCCTAATAGTTATGGTATAAAAACTTTTTCGGCAAAGACATTACTTCCTCTTTTGAGTGGCTCCGTCAAGGTATCCTACCGTTCTAGGACCATAGCCAGACGCTAAGGCTGAGCAGGT3,5203,5403,5603,5803,6003,6203,6403,660
    ACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACTGTAGTTATGTTGGATAATTAAAGGGGAGCAGTTTTTATTCCAATAGTTCACTCTTTAGTGGTACTCACTGCTGACTTAGGCCACTCTTACCGTTTTCAAATACGTAAAGAAAGGTCTGAACAAGTTGTCCGGTCGGTAATGCGAGCAGTAGTTTTAGTGAGCGTAGTTGGTTTGNsiI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATGCAATAAGTAAGCACTAACGCGGACTCGCTCTGCTTTATGCGCTAGCGACAATTTTCCTGTTAATGTTTGTCCTTAGCTTACGTTGGCCGCGTCCTTGTGACGGTCGCGTAGTTGTTATAAAAGTGGACTTAGTCCTATAAGAAGATTATGGACCTTACGACAAAAGGGCCCCTAAjuIPvuISmaIXmaIAsiSITspMI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATGCGTCACCACTCATTGGTACGTAGTAGTCCTCATGCCTATTTTACGAACTACCAGCCTTCTCCGTATTTAAGGCAGTCGGTCAAATCAGACTGGTAGAGTAGACATTGTAGTAACCGTTGCGATGGAAACGGTACAAAGTCTTTGTTGAGACCGCGTAGCCCGAAGGGTATGTTANsiI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGGCTATCTAACAGCGTGGACTAACGGGCTGTAATAGCGCTCGGGTAAATATGGGTATATTTAGTCGTAGGTACAACCTTAAATTAGCGCCGGATCTCGTTCTGCAAAGGGCAACTTATACCGAGTATTGTGGGGAACATAATGACAAATACATTCGTCTGTCAAAATAACAAGTACClaINruIBspDI4,2204,2404,2604,2804,3004,3204,3404,360
    ACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAG4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAG4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCC4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAA4,9204,9404,9604,9805,0005,0205,0405,060
    GCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTCGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGAPciISapIBspQIAflIII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCCACGCCATAAAGTGTGGCGTATATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAccIBstZ17I5,2605,2805,3005,3205,3405,3605,3805,4005,420
    TTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTCAAAGAGGTCTTCGCAATTA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGCAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAATGACCTTGCAACAC5,6205,6405,6605,6805,7005,7205,7405,760
    AGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTATCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAGGTCTGAAATBspEI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCTTTGTGCCTTTGGCTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGCACCCFspIFspAIPpuMI5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACGGCGGTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGT6,1406,1606,1806,2006,2206,2406,2606,2806,300
    CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCGGACAGGATGCTCAACGTACTATTTCTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGPshAI6,3206,3406,3606,3806,4006,4206,4406,460
    TTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGABsaXI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCCCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCATAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGHpaIEcoRVBssHIIHincII6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCApaIPspOMI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTGCTAAACGACCACTGGGTTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAABstEII7,0207,0407,0607,0807,1007,1207,1407,160
    AATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTMluIAflIII7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCkkknnCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGmmmnnGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACG7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGTCATCATCCAACTCCGGCAA7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGCTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTACCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACACSphI7,7207,7407,7607,7807,8007,8207,8407,860
    GCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCSgrAI7,8807,900
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7918 bp5500600065007000750050010001500Cas13b-t3dCas13b-t3-IF-fwIF459-2786453-458Cas13bt3-allASL-GGGSx2-r (DNA)6xHis,SUMO,XbaI,NcoI,KflI,PpuMI,SbfI,DraI,AflII,EcoRIpSUMO-IF-Rv,TEV,Linker,BseRI,HincIIHEPN1,PspOMI,ApaId,d,d,ddSexAI,BsrGIPmlI,BmgBIPmlITspMI,XmaI,SmaIZraI,AatII,BsrGISexAIPpuMIFspAIFspIPshAIBsaXIHincII,HpaIEcoRVBssHIIPspOMI,ApaI,BstEIIAflIIIMluISphI,SgrAI

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    7918