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pBT271.359 APOBEC3A deaminase donor for Golden Gate
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    gctcttctagtgaagccagcccagcatccgggcccagacacttgatggatccacacatattcacttccaactttaacaatggcattggaaggcataagacctacctgtgctacgaagtggagcgcctggacaatggcacctcggtcaagatggaccagcacaggggctttctacacgagaagatcacttcggtcgggtcgtaggcccgggtctgtgaactacctaggtgtgtataagtgaaggttgaaattgttaccgtaaccttccgtattctggatggacacgatgcttcacctcgcggacctgttaccgtggagccagttctacctggtcgtgtccccgaaagatgtApaIEarISapIBamHIBspQIPspOMIS1EA2SP4AS6GP8RH10LM12DP14HI16FT18SN20FN22NG24IG26RH28KT30YL32CY34EV36ER38LD40NG42TS44VK46MD48QH50RG52FL54H12-605hAPOBEC3A isoform A20406080100120140160
    caaccaggctaagaatcttctctgtggcttttacggccgccatgcggagctgcgcttcttggacctggttccttctttgcagttggacccggcccagatctacagggtcacttggttcatctcctggagcccctgcttctcctggggctgtgccggggaagtgcgtgcgttccttgttggtccgattcttagaagagacaccgaaaatgccggcggtacgcctcgacgcgaagaacctggaccaaggaagaaacgtcaacctgggccgggtctagatgtcccagtgaaccaagtagaggacctcggggacgaagaggaccccgacacggccccttcacgcacgcaaggaaEaeIEagIBglIISexAIN56QA58KN60LL62CG64FY66GR68HA70EL72RF74LD76LV78PS80LQ82LD84PA86QI88YR90VT92WF94IS96WS98PC100FS102WG104CA106GE108VR110AF112L12-605hAPOBEC3A isoform A180200220240260280300320340
    caggagaacacacacgtgagactgcgcatcttcgctgcccgcatctatgattacgaccccctatataaggaggcgctgcaaatgctgcgggatgctggggcccaagtctccatcatgacctacgatgagttcaagcactgctgggacacctttgtggaccaccagggatgtccctgtcctcttgtgtgtgcactctgacgcgtagaagcgacgggcgtagatactaatgctgggggatatattcctccgcgacgtttacgacgccctacgaccccgggttcagaggtagtactggatgctactcaagttcgtgacgaccctgtggaaacacctggtggtccctacagggaAleIAleIApaIBtsIFspIPmlIBcoDIBcoDIBsaAIBsmAIBsmAIBtsαIEcoNIBstAPIPspOMIEcoO109IQ114EN116TH118VR120LR122IF124AA126RI128YD130YD132PL134YK136EA138LQ140ML142RD144AG146AQ148VS150IM152TY154DE156FK158HC160WD162TF164VD166HQ168GC170P12-605hAPOBEC3A isoform A360380400420440460480500520
    tccagccctgggatggactagatgagcacagccaagccctgagtgggaggctgcgggccattctccagaatcagggaaactctggctgaagagcctggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcactaggtcgggaccctacctgatctactcgtgtcggttcgggactcaccctccgacgcccggtaagaggtcttagtccctttgagaccgacttctcggaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaEarIPasISapISspIBspQICspCIS1G2F172QP174WD176GL178DE180HS182QA184LS186GR188LR190AI192LQ194NQ196GN19812-605hAPOBEC3A isoform A540560580600620640660680700
    tttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcAtgaattaattcTtaaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagTacttaattaagAattggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggtAseIXmnIBciVIBsrBIterminatorUSE...27720740760780800820840860
    agcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacggggtcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgccccBtgINcoISacIScaISspIStyITatIBtgZIEcoRVEco53kIchloramphenicol acetyltransferase CmRterminator8809009209409609801,0001,0201,040
    gcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaaacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtcgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactttgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaDraIEaeIMscIPasIPflMIchloramphenicol acetyltransferase CmR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaaAclIBspEIBsrDIBtgZIchloramphenicol acetyltransferase CmR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttDraIPvuIIBpu10Ichloramphenicol acetyltransferase CmRpromoter1,4201,4401,4601,4801,5001,5201,5401,560
    aatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaatcAtgaccaaaaTcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttagTactggttttAgggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaaBsaAIpUC originpromoterUS...51,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtTcttctagtgtagccgtagttaggccaccacttcaagaactctggacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaAgaagatcacatcggcatcaatccggtggtgaagttcttgagacpUC origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtgAlwNIApaLIpUC origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaaEciIBciVIBssSIBssSαIpUC origin2,1202,1402,1602,1802,2002,2202,2402,260
    gagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttActgcgttaTgtagtcaaaagcctccggtcggaggcttttgacttggctgaggagtgccatctcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaTgacgcaatAcatcagttttcggaggccagcctccgaaaactgaaccgactcctcacggtaDrdIEciINspIPciIBbvCIBpu10IpUC origintermbidirectional t...ward directionChen, Voigt et...l terminator)Chen, Voigt e...aracterized)hairpinUS...62,2802,3002,3202,3402,3602,3802,4002,4202,440
    taagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtcttattcgtggccacctcactgctggaagtcgtgcaagcatgacaagttgctaccacatcagaagcaacaccctccactacaggtcaaactacagccaaaacattcgtgggccgtcgacttggccaaaaaatcggtacatccaccaaaattgaagtcgcagcatcactggtggcagaaAgeIAgeIBsaXIBsrFIBsrFIBstXIPshAIPvuIISgrAImRFP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcagtcaaagtctgcgtaaaactaaagtggaaagtctcgtggcagaaggcccatgtatgcaagccaccttcgaagggttgggtaccaaaaaaagacgtattggcctggcagcctgcccttcaaccatggtgcgtcaaattgaaacatctacttgagtggcagaacgtccctcctcagBaeIBtgIKpnINcoIStyIPflMIRsrIIAcc65IHindIIImRFP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccagacccattgccattgttgtggtggcagaagcttcaagtattgtgcaagggtaaactttggaaggcccttcctgtcaaagtccatcaggccctacagtcggcccacaaattgcattcgaaaccttggcatgaccttgacgcccctgtcctacagggttcgcttgccgtcgcctggtAhdIBstBIHindIIImRFP2,8202,8402,8602,8802,9002,9202,9402,960
    cctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcgctactcgccatagatcctttctcctctttcAgatggaaaccattgaaagtcaaatcgccagacccatggaagcatgcctgctggaagtggaagtggaagctaaagcttgagcactggcaattgccttggaaggtatgcttgaaactttgcgtacttgagaaactattgcagaagcgatgagcggtatctaggaaagaggagaaagTctaBaeIHpaIKpnIPsrIBsiWIBssSIBstBIAcc65IBssSαIHincIImRFPRBS2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccgTgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggAAgggtGAcTcgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtgagtcagtgagcgagaatggcAcgagtcatagagatagtgactatccctacagttagagatagtgactatcccTTcccaCTgAgcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcactcagtcactcgctcttaBsaBIBsrBIpTetBBa_R0040tetOtetO3,1603,1803,2003,2203,2403,2603,280
    • Plasmid
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    • Linear Map
    Editing is disabled because sequence edits have been locked.
    3289 bpAPOBEC3A deaminasepBT300/pBT271 backbone2250250027503000250500750mRFPterminatorGGC for SapI Golden Gate cloningtetO9-11606-61112-605BT-567F (DNA)BT-525SF (DNA)BT-14.D01 (DNA)BT-741F 12.G09 (DNA)

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    3289