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Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette
  • Sequence Map
    Editing disabled on read-only sequence.
    ggttaccccagcccctcgagcgtaggtgcctctctttcgttcgcctggtgccgtctctttctgcgagggggagggatcttgcgcacgtatatgaataattcaactgatcgccgtggtgattgggggatgaacggagtactggtttgtccgttcctcggaggtgcggaatgcacagccaatggggtcggggagctcgcatccacggagagaaagcaagcggaccacggcagagaaagacgctccccctccctagaacgcgtgcatatacttattaagttgactagcggcaccactaaccccctacttgcctcatgaccaaacaggcaaggagcctccacgccttacgtgtcFspITliIXhoIPspXIPaeR7Irunt20406080100120140160
    atcgtagttctgatacccattcatttcgaggaaatttattcaggaacgtcacggtatttgcataagtgaaatcgtatctcacagttagcagcctgtatcgtagattgattactaaaatacttttctaaataatctgcactaagatatagttcagattgcgtaagatcggtaagtatagcatcaagactatgggtaagtaaagctcctttaaataagtccttgcagtgccataaacgtattcactttagcatagagtgtcaatcgtcggacatagcatctaactaatgattttatgaaaagatttattagacgtgattctatatcaagtctaacgcattctagccattcatrunt180200220240260280300320340
    cagaagcttttaatcgcactggaagttttatttaccgctcacgacatttgcatagatgaaaccgtatcttacagattcaagtagcttgcgtactaaactactgtacaaaaaatatctgcactaagaaatagtcgggagtgattttttatggtgaagtacagaagtaaattcagctgtcttcgaaaattagcgtgaccttcaaaataaatggcgagtgctgtaaacgtatctactttggcatagaatgtctaagttcatcgaacgcatgatttgatgacatgttttttatagacgtgattctttatcagccctcactaaaaaataccacttcatgtcttcatttaagtcgaHindIII360380400420440460480500520
    aattaagcctcgctcttttttgttttaaattaactgctcaacagcagtgagaaaaacattgcatatattgagtacatgaatacaggttactgtcgccttaatctcccatcggttaatcccgctaaaaagcgaagtcctttgagtttttggtggccaggtaggcactttccgtatcttaattcggagcgagaaaaaacaaaatttaattgacgagttgtcgtcactctttttgtaacgtatataactcatgtacttatgtccaatgacagcggaattagagggtagccaattagggcgatttttcgcttcaggaaactcaaaaaccaccggtccatccgtgaaaggcatag540560580600620640660680700
    agatgctcgttgcttattttttgggaacatattttatggcccgtggcggcggctaatcggccaaaatatttgcggggcgtgctgcttaatccgggcgattgactttcataagcaaaggattaagattgcgcggttggactacctgttttgaggtgcgatatcagtacatgcgatgtctacgagcaacgaataaaaaacccttgtataaaataccgggcaccgccgccgattagccggttttataaacgccccgcacgacgaattaggcccgctaactgaaagtattcgtttcctaattctaacgcgccaacctgatggacaaaactccacgctatagtcatgtacgctacEcoRV720740760780800820840860
    gtacatctgagggccaggtacgtcaaagccagtaaacccatagttttcccacttttttggggccgcaaaaaagcatcggagggcccataaaaaaagggttgatccttttggctgctgtgggctcgtagcgagttcggtatggagatcaggtactgcctggtgctcggtgatccctcatgtagactcccggtccatgcagtttcggtcatttgggtatcaaaagggtgaaaaaaccccggcgttttttcgtagcctcccgggtatttttttcccaactaggaaaaccgacgacacccgagcatcgctcaagccatacctctagtccatgacggaccacgagccactagggaApaIPspOMI8809009209409609801,0001,0201,040
    atgaggcggtcctgcgggtcctgcgatgcgcgtgctgcggcagctcctgtcgcaaattgccaaggaatcgcagcaggatccaagaagcgacgacaggagcgctgatttcccgggaaaccggcaatcgtcaatcggccatcggcaatcgcgtccttgtcgcacgcccgctaaaccttactccgccaggacgcccaggacgctacgcgcacgacgccgtcgaggacagcgtttaacggttccttagcgtcgtcctaggttcttcgctgctgtcctcgcgactaaagggccctttggccgttagcagttagccggtagccgttagcgcaggaacagcgtgcgggcgatttggaAfeISmaIXmaIBamHITspMI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gcgctgtcctgccatatatcccggggctatatggtgtgaaatcggtgtagggacacgaggtccttcgcagcgagcggccgcgcacgtacaaaaggcagcgctgccgatacactggatttactggaagtgtgcgtctataatgtccagctgagagcggcgcgttctcgcgacatttcgcgacaggacggtatatagggccccgatataccacactttagccacatccctgtgctccaggaagcgtcgctcgccggcgcgtgcatgttttccgtcgcgacggctatgtgacctaaatgaccttcacacgcagatattacaggtcgactctcgccgcgcaagagcgctgtaaaAfeIAhdINruISmaIXmaITspMI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tggttggtcttgcctcgtagttcccctaccagaatggccatgctccccattccttctcctcctcctccacgcggtgcctggaagagtccgccacctcggaaaactcgcgggtcctgtgtggcctcgtttttaaggttgttttggtgggccgcaattttttggccagtcaggcactaccaaccagaacggagcatcaaggggatggtcttaccggtacgaggggtaaggaagaggaggaggaggtgcgccacggaccttctcaggcggtggagccttttgagcgcccaggacacaccggagcaaaaattccaacaaaaccacccggcgttaaaaaaccggtcagtccgtgaEarIDraIII1,4201,4401,4601,4801,5001,5201,5401,560
    tgatttgcgttccactgcacgaaatgagtgttgaaggaattgagaatattactttaaattgtcaagtcaagtgtcaagtgttattcagcagctcttaagaattaatacacagttaatccattttacattaagttaattaaatcgttcatatttcgtttttgaagctaagtttcgtactaaacgcaaggtgacgtgctttactcacaacttccttaactcttataatgaaatttaacagttcagttcacagttcacaataagtcgtcgagaattcttaattatgtgtcaattaggtaaaatgtaattcaattaatttagcaagtataaagcaaaaacttcgattcaaagcaPacIAflII1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aatctgaattactatctatgtatgtgctaatcacttagccaatcgctaagcaaaatgtacaaatcttaaaacctaagtaaacgtagattagtttgaagagagttcaatgtaggcagccaaagatactcgatgttcttccaagtatgacacttccgcatcagattaaatccgatcattagacttaatgatagatacatacacgattagtgaatcggttagcgattcgttttacatgtttagaattttggattcatttgcatctaatcaaacttctctcaagttacatccgtcggtttctatgagctacaagaaggttcatactgtgaaggcgtagtctaatttaggctagtEarI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gctagctaaacctgatccaatcaaggatcggcgatcgcaggcagcgaaaatggaaatgaaatcgagccggattgcgccacacgaactgagaggcgcacaaatatttacaggactaatcgcaggataatcgcccgatcagcattaggattaagttcgatccccggagagatcgatgcgatcgatttggactaggttagttcctagccgctagcgtccgtcgcttttacctttactttagctcggcctaacgcggtgtgcttgactctccgcgtgtttataaatgtcctgattagcgtcctattagcgggctagtcgtaatcctaattcaagctaggggcctctctagctacBmtINheIAsiSI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcactgcatcttagatagtactggaagtggaccggacctgacaatgggtgttagttggacagattcagatcggagattgcagatcagttgatcgagttccgagttccgagatcggcgataaagtaaattttgggcggaatcgagaaaaacattgagcaacactgagggatcagtgcgtgacgtagaatctatcatgaccttcacctggcctggactgttacccacaatcaacctgtctaagtctagcctctaacgtctagtcaactagctcaaggctcaaggctctagccgctatttcatttaaaacccgccttagctctttttgtaactcgttgtgactccctagtcac2,1202,1402,1602,1802,2002,2202,2402,260
    tgccgtaatatgatcagccggggatttctgattattttgaatgaaacactaatttttgggacattttcgaacaatgggacgcactgttatgcacgtgtatatgtttattaattagatctgatcttaaattacttaacttaacttatttgaaatgaaattatatggataacgttgaacggcattatactagtcggcccctaaagactaataaaacttactttgtgattaaaaaccctgtaaaagcttgttaccctgcgtgacaatacgtgcacatatacaaataattaatctagactagaatttaatgaattgaattgaataaactttactttaatatacctattgcaactAclIPmlI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgcatattcaaaaaaaaaaaaattaacactggaaatgattagttttttgaacagattattaaaacgatttgtagcactaataatgatgtatttaaaatatcgtgcttatgttgatatatatattatatatatatatatatatatattattatatttattatatttttttcggcatacgtataagtttttttttttttaattgtgacctttactaatcaaaaaacttgtctaataattttgctaaacatcgtgattattactacataaattttatagcacgaatacaactatatatataatatatatatatatatatatataataatataaataatataaaaaaagccgta2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cttcatcttaatcgataagataccttcgaaagcaattaaatatatgataataaatacaaatataaaggaaaatataattcatcttatgatagtatgggatagtgtgtactatatgtactatagattattatcataattgaaaatcccatgttccaaaattttccaaactccacaagaagtagaattagctattctatggaagctttcgttaatttatatactattatttatgtttatatttccttttatattaagtagaatactatcataccctatcacacatgatatacatgatatctaataatagtattaacttttagggtacaaggttttaaaaggtttgaggtgttBaeI2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgtatcacccatgaaattaaaaggcttaaatccttttatcccaaagtcatagtgatatagctctcctgatgcttgtgataatatttatgataatttcgcaacctgatccctcctcgcactgactaattgagtcctcatgtcctgtcggcaggacgcccctggcatcctagctacagcatagtgggtactttaattttccgaatttaggaaaatagggtttcagtatcactatatcgagaggactacgaacactattataaatactattaaagcgttggactagggaggagcgtgactgattaactcaggagtacaggacagccgtcctgcggggaccgtaggatcgatg2,8202,8402,8602,8802,9002,9202,9402,960
    tcgggaaagggaaggcaagggaacgagaaggagatggtgaagggaaccccccgtttggccatgggtgcctacctgtaccacccgcccccgcccccctcgacgaacccctccgcaggagggccgccaaaacacgcgcgccgctggaagtggaaaatgggaaactgttgcacggccgagccctttcccttccgttcccttgctcttcctctaccacttcccttggggggcaaaccggtacccacggatggacatggtgggcgggggcggggggagctgcttggggaggcgtcctcccggcggttttgtgcgcgcggcgaccttcaccttttaccctttgacaacgtgccggcNcoIRuntN-LH2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tttttcttttggcgtgcgtggcgaaaaaataaaattcaaagcgaagaaaaggaaaacgcacacaatttccagaccgcccgtggggaaaagcgagagaggaagcgagagccagagcgagagagtggaaaagtctcggtgtatttcttttttgagtttttctttggtgcaatttccaaaaaagaaaaccgcacgcaccgcttttttattttaagtttcgcttcttttccttttgcgtgtgttaaaggtctggcgggcaccccttttcgctctctccttcgctctcggtctcgctctctcaccttttcagagccacataaagaaaaaactcaaaaagaaaccacgttaaaggtRuntN-LH3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggccatcactctctctctctctttctcgctctcgcccatgcgattttccttttttccgctgttattttttcctttttggccacaagtaacaagcgacttctctctcacgtacacaggcacactaacacgacagccaaacacacacacacacacaaacacacagagagaccgcaatccggtagtgagagagagagagaaagagcgagagcgggtacgctaaaaggaaaaaaggcgacaataaaaaaggaaaaaccggtgttcattgttcgctgaagagagagtgcatgtgtccgtgtgattgtgctgtcggtttgtgtgtgtgtgtgtgtttgtgtgtctctctggcgttaBsaIRuntN-LHrunt3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggaaaaatgggaaattttttgctgtggttgttgttgcggcggcaatggtggttgctcgatataccgatatatacctgccccggcacccttaaaggcgcgcctgtgtgcgtaggtagccaaccacaccaccataccgcccaggataatgggcgggaaagagacggcgctagcgccgcctttttaccctttaaaaaacgacaccaacaacaacgccgccgttaccaccaacgagctatatggctatatatggacggggccgtgggaatttccgcgcggacacacgcatccatcggttggtgtggtggtatggcgggtcctattacccgccctttctctgccgcgatcgcggcAscIBmtINheIRuntN-LHrunt3,5203,5403,5603,5803,6003,6203,6403,660
    tgcaagtgggacggggcacaaacgccggcagagccgtcgacagaggcggcgagcagcgcattccactttaaatactgccaacgatcgcagcagagaatcagttcactcagcgactttagcgtgtcgagaacgcacagagaaatatatcgttttcgactttggaaaacagaaacagacgttcaccctgccccgtgtttgcggccgtctcggcagctgtctccgccgctcgtcgcgtaaggtgaaatttatgacggttgctagcgtcgtctcttagtcaagtgagtcgctgaaatcgcacagctcttgcgtgtctctttatatagcaaaagctgaaaccttttgtctttgtcXmnIRuntN-LHrunt3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gctcgaaagaagcagagttcgaaatcgcgaaacaatccaataacaacaaacaaacgaattttttccaagtgacccccgataaagtgaagtgcataccgagaatccgcaatagaaaaccggcaattgtcgacagccccaggattacggctacgatttccacattcggatacgagTTcgagctttcttcgtctcaagctttagcgctttgttaggttattgttgtttgtttgcttaaaaaaggttcactgggggctatttcacttcacgtatggctcttaggcgttatcttttggccgttaacagctgtcggggtcctaatgccgatgctaaaggtgtaagcctatgctcAANruIXmnIruntRuntN-LHgRNA-Runt-N-43,8603,8803,9003,9203,9403,9603,9804,0004,020
    AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGAACTATAACGTTGGGATCTTTCTATCAGACGCATTTTAACTGCGTACGTAAGAACTTTATAACGAGAGAGAAAGATTTATCGCGCTTAGGCAGCGACACGTAAATCCTGTAGAGTCAGCGGCGAACCTCGAGGGCACTCCGCACGAACAGTTACGCCATTCACAGTGACTAAAACTTGATATTGCDrdIHpaIrunt5'transposon end4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ACCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACGATAATTATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTAtatatatattttcttgttatagatatctaagatacattgatgagtttggacaaaccacaactagaaTGGCGCACTCAGTTTTACTGCGTACTAATAGAAAATGCACTGAAAATTCTAAATTGAGTATGCTATTAATATAACAATAAAGTACAAGATGAATGCACTATTGAATAATatatatataaaagaacaatatctatagattctatgtaactactcaaacctgtttggtgttgatcttEcoRVrunt5'transposon endSV40 polyA4,2204,2404,2604,2804,3004,3204,3404,360
    tgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgacgttatttgttcaattgttgttgttaacgtaagtaaaatacaaagtccaagtccccctccacaccctccaaaaaatttcgttcattttggagatgtttacaccataHpaIruntSV40 polyA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggctgattatgatctagagtcgcggccgctacaggaacaggtggtggcggccctcggcgcgctcgtactgctccacgatggtgtagtcctcgttgtgggaggtgatgtccagcttggagtccacgtagtagtagccgggcagctgcacgggcttcttggccatgtagatggacttccgactaatactagatctcagcgccggcgatgtccttgtccaccaccgccgggagccgcgcgagcatgacgaggtgctaccacatcaggagcaacaccctccactacaggtcgaacctcaggtgcatcatcatcggcccgtcgacgtgcccgaagaaccggtacatctacctgaaAleIXbaI*226LF224LH222HR220GE218AR216EY214QE212VI210TY208DE206NH204ST202ID200LK198SD196VY194YY192GP190LQ188VP186KK184AM182YI180SK1784579-5256runtdsRed2SV40 polyA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaactccaccaggtagtggccgccgtccttcagcttcagggccttgtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggacgcctcccagcccatagtcttcttctgcattacggggccgtcggaggggaagttcacgccgatgaacttcaccttgtagcttgaggtggtccatcaccggcggcaggaagtcgaagtcccggaacacctagagcgggaagtcgtgcggcagcgcccccatgtccgcgagccacctgcggagggtcgggtatcagaagaagacgtaatgccccggcagcctccccttcaagtgcggctacttgaagtggaacatcAloIBbsISexAIFE176VL174YH172GG170DK168LK166LA164KH162IE160GK158LV156GD154RP152YL150RE148TS146AE144WG142MT140KK138QM136VP134GD132SP130FN128VG126IF124KV122KY1204579-5256runtdsRed24,7404,7604,7804,8004,8204,8404,8604,8804,900
    atgaaggagccgtcctggagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcttgtagtcggggatgtcggcggggtgcttcacgtacaccttggagccgtactggaactggggggacatacttcctcggcaggacctccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacttcgggagccccttcctgtcgaagaacatcagcccctacagccgccccacgaagtgcatgtggaacctcggcatgaccttgacccccctgtIF118SG116DQ114LS112SD110QT108VT106VV104GG102DE100FN98MV96RE94WK92FG90EP88FS86LK84KY82DP80ID78AP76HK74VY72VK70SG68YQ66FQ64PS62L4579-5256runtdsRed24,9204,9404,9604,9805,0005,0205,0405,060
    ggatgtcccaggcgaagggcagggggccgcccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgcgcaccttgaagcgcatgaactccttgatgacgtcctcggaggacctacagggtccgcttcccgtcccccggcgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggagcgggagctagagcttgagcaccggcaagtgcctcgggaggtacgcgtggaacttcgcgtacttgaggaactactgcaggagcctcctAhdIFspIZraIAatIIFspAII60DW58AF56PL54PG52GK50TV48KL46KA44TQ42TG40EY38PR36GE34GE32GE30IE28FE26HG24NV22SG20EM18RV16KF14RM12FE10KI8VD6ES4S4579-5256runtdsRed25,0805,1005,1205,1405,1605,1805,2005,2205,240
    ggccatggtggcgaccggcttcgagccgattgtttagcttgttcagctgcgcttgtttatttgcttagctttcgcttagcgacgtgttcactttgcttgtttgaattgaattgtcgctccgtagacgaagcgcctctatttatactccggcggtcgagggttcgaaatcgataagccggtaccaccgctggccgaagctcggctaacaaatcgaacaagtcgacgcgaacaaataaacgaatcgaaagcgaatcgctgcacaagtgaaacgaacaaacttaacttaacagcgaggcatctgcttcgcggagataaatatgaggccgccagctcccaagctttagctattcNcoIHindIIIA2Mrunt3xP3 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cttggatcctaattgaattagctctaattgaattagtctctaattgaattagatcctaaaagttttgttactttatagaagaaattttgagtttttgtttttttttaataaataaataaacataaataaattgtttgttgaatttattattagtatgtaagtgtaaataTAATAAgaacctaggattaacttaatcgagattaacttaatcagagattaacttaatctaggattttcaaaacaatgaaatatcttctttaaaactcaaaaacaaaaaaaaattatttatttatttgtatttatttaacaaacaacttaaataataatcatacattcacatttatATTATTBamHIrunt3'transposon end3xP3 promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    AACTTAATATCTATTCAAATTAATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCACAATATGATTATCTTTCTAGGGTTAAATGGCCCAGGTTCAGCTGGTCGAGTCCGGTGGTGCCCTGGTTCAGCCCGGTGTTGAATTATAGATAAGTTTAATTATTTATTTGGAGCTATATGTCTGGCTATTTTGTGTACGCAGTTAAAATGCGTACTAATAGAAATTGCATGCAGTGTTATACTAATAGAAAGATCCCAATTTACCGGGTCCAAGTCGACCAGCTCAGGCCACCACGGGACCAAGTCGGGCCACBsiWIMA2QV4QL6VE8SG10GA12LV14QP16G5724-6074runt3'transposon endJB3Nanobodysame as JB105,6205,6405,6605,6805,7005,7205,7405,760
    GCAGCCTGCGCCTGTCCTGCGCCGCCAGCGGTTTCCCAGTGAACCGTTATTCGATGCGCTGGTACCGTCAGGCCCCCGGAAAGGAACGCGAGTGGGTGGCCGGCATGTCCTCCGCCGGCGATCGCTCCAGCTATGAGGATAGCGTCAAGGGCCGTTTCACTATCTCCCGCGACGACGTCGGACGCGGACAGGACGCGGCGGTCGCCAAAGGGTCACTTGGCAATAAGCTACGCGACCATGGCAGTCCGGGGGCCTTTCCTTGCGCTCACCCACCGGCCGTACAGGAGGCGGCCGCTAGCGAGGTCGATACTCCTATCGCAGTTCCCGGCAAAGTGATAGAGGGCGCTGCTKpnIMreIAsiSISgrAIAcc65IG18SL20RL22SC24AA26SG28FP30VN32RY34SM36RW38YR40QA42PG44KE46RE48WV50AG52MS54SA56GD58RS60SY62ED64SV66KG68RF70TI72SR74DD765724-6074runtJB3Nanobody5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CGCCCGCAACACCGTCTATTTGCAGATGAACTCCCTGAAGCCCGAGGACACTGCCGTCTACTACTGCAACGTCAACGTCGGATTCGAGTACTGGGGCCAGGGCACTCAGGTCACTGTCTCGTCGggcggaggtggcggtggaatgcatctgccagcgggtccaacgatggtggccGCGGGCGTTGTGGCAGATAAACGTCTACTTGAGGGACTTCGGGCTCCTGTGACGGCAGATGATGACGTTGCAGTTGCAGCCTAAGCTCATGACCCCGGTCCCGTGAGTCCAGTGACAGAGCAGCccgcctccaccgccaccttacgtagacggtcgcccaggttgctaccaccggDrdICspCIG1G2G3G4G5G66075-6092MH2LP4AG6PT8MV10A6093-6792AR78NT80VY82LQ84MN86SL88KP90ED92TA94VY96YC98NV100NV102GF104EY106WG108QG110TQ112VT114VS116S5724-6074runtJB3NanobodyRuntRuntN-RHgRNA-Runt-N-2gRNA-Runt-N-16XGly linker5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aacaacacacaggtcctggccgctgccgccgccgcagcagccgccgcagcggccgcggtagcccagggtcctgggccgcagcagagtagcaatgccaccaccgcctccgcaatcgccatcaatcccgcccagagtctggccaacacgagcacccactcggccagcagcacgggcattgttgtgtgtccaggaccggcgacggcggcggcgtcgtcggcggcgtcgccggcgccatcgggtcccaggacccggcgtcgtctcatcgttacggtggtggcggaggcgttagcggtagttagggcgggtctcagaccggttgtgctcgtgggtgagccggtcgtcgtgcccgtPasISacIIN12NT14QV16LA18AA20AA22AA24AA26AA28AA30VA32QG34PG36PQ38QS40SN42AT44TA46SA48IA50IN52PA54QS56LA58NT60ST62HS64AS66ST68G6093-6792runtRuntRuntN-RHgRNA-...new)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gctccacaccagatctcagcacgaacaacaccagctcgagcagcaacgccaccaccagtccgcagaactccgccaagatgcccagctcgatgaccgacatgttcgccagcctgcacgagatgctgcaggagtatcacggcgagctggcccagaccggatcgccctcgatcctctgcgaggtgtggtctagagtcgtgcttgttgtggtcgagctcgtcgttgcggtggtggtcaggcgtcttgaggcggttctacgggtcgagctactggctgtacaagcggtcggacgtgctctacgacgtcctcatagtgccgctcgaccgggtctggcctagcgggagctaggagacPciIPstITliIXhoIPspXIPaeR7IS70ST72PD74LS76TN78NT80SS82SS84NA86TT88SP90QN92SA94KM96PS98SM100TD102MF104AS106LH108EM110LQ112EY114HG116EL118AQ120TG122SP124SI126LC1286093-6792runtRuntRuntN-RH6,3206,3406,3606,3806,4006,4206,4406,460
    cagcgccctgcccaatcactggcggtcgaacaagtcgctgcccggagccttcaaggtgatcgccctggacgacgtgcccgatggcaccctggtgtccatcaagtgcggcaacgacgaaaactactgcggcgagctgaggaactgcaccacgaccatgaagaaccaggtggccaaggtcgcgggacgggttagtgaccgccagcttgttcagcgacgggcctcggaagttccactagcgggacctgctgcacgggctaccgtgggaccacaggtagttcacgccgttgctgcttttgatgacgccgctcgactccttgacgtggtgctggtacttcttggtccaccggttcAleIPstIBbvCISexAIBpu10ISA130LP132NH134WR136SN138KS140LP142GA144FK146VI148AL150DD152VP154DG156TL158VS160IK162CG164ND166EN168YC170GE172LR174NC176TT178TM180KN182QV184AK1866093-6792runtRuntRuntN-RH6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttcaacgatctgcgcttcgtgggacgatcgggacgcggcaagtccttcacgctgaccatcaccattgccacctatccggtgcagatcgccagctacagcaaagccatcaaggtgaccgtcgacgggccacgggagccaagaagtaagcaaagtgagttttttagtttccattatgaagttgctagacgcgaagcaccctgctagccctgcgccgttcaggaagtgcgactggtagtggtaacggtggataggccacgtctagcggtcgatgtcgtttcggtagttccactggcagctgcccggtgccctcggttcttcattcgtttcactcaaaaaatcaaaggtaatacSgrDIFN188DL190RF192VG194RS196GR198GK200SF202TL204TI206TI208AT210YP212VQ214IA216SY218SK220AI222KV224TV226DG228PR230EP232R6093-6792RuntN-RHruntRunt6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttttcagtttaatccactcccaaagatccccaaaaaaccaattcagaaaccgtttgcatatcttcaattacaatattcatagttttgaaaacatttgccaacactaactagttatgatcttaaataactaatgccatttttaagctcaactttaaatgatcagtctgttttgaaaaaaagtcaaattaggtgagggtttctaggggttttttggttaagtctttggcaaacgtatagaagttaatgttataagtatcaaaacttttgtaaacggttgtgattgatcaatactagaatttattgattacggtaaaaattcgagttgaaatttactagtcagacaaaactttRuntN-RH6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tgattgcattgatcactttatcttaaagattttatcattatttttgcttatccttttgcaattcggttttgatacacatatccaaggatcttgagatccattgccgccaaccaaatcccccaccatccccccaacctttttaaaaacgcattccgatgaaatacgagttgtgcttactaacgtaactagtgaaatagaatttctaaaatagtaataaaaacgaataggaaaacgttaagccaaaactatgtgtataggttcctagaactctaggtaacggcggttggtttagggggtggtaggggggttggaaaaatttttgcgtaaggctactttatgctcaacacgaaBpuEIRuntN-RH7,0207,0407,0607,0807,1007,1207,1407,160
    tactgacttcccgccctttttctttatccttccaggctatggctatccccatcccggcgcgtttaacccgttcatgctgaatcccgcttggctggatgcggcgtacatgacctacggctatgcggactacttccgccaccaggcagccgcccaggcggcccaggtgcatcatccgatgactgaagggcgggaaaaagaaataggaaggtccgataccgataggggtagggccgcgcaaattgggcaagtacgacttagggcgaaccgacctacgccgcatgtactggatgccgatacgcctgatgaaggcggtggtccgtcggcgggtccgccgggtccacgtagtaggcYG2YP4HP6GA8FN10PF12ML14NP16AW18LD20AA22YM24TY26GY28AD30YF32RH34QA36AA38QA40AQ42VH44HP467213-8034Runtrunt7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gcactggccaagtcctcggcttcctcggtgtcgccaaatcccaatccatcggtggccaccagctcctcgtcggcggttcagccatctgagtatccccatccggcggcagcagtggcagcggcggcgggacaaccggcggccatgatgccatcgccaccgggagcggcgcctgccacgtgaccggttcaggagccgaaggagccacagcggtttagggttaggtagccaccggtggtcgaggagcagccgccaagtcggtagactcataggggtaggccgccgtcgtcaccgtcgccgccgccctgttggccgccggtactacggtagcggtggccctcgccgcggacggtAL48AK50SS52AS54SV56SP58NP60NP62SV64AT66SS68SS70AV72QP74SE76YP78HP80AA82AV84AA86AA88GQ90PA92AM94MP96SP98PG100AA102PA1047213-8034Runtrunt7,3607,3807,4007,4207,4407,4607,4807,5007,520
    cgccctatgccattccgcagttcccattcaaccatgtggccgccgcagccgccgccaaggcagccacgccacacgccttccatccgtataactttgccgcggcagctggcttgcgggcccgcaatgctgccctgcaccaccagagcgagccggtccacgtcagccccgcctcctcgcgggatacggtaaggcgtcaagggtaagttggtacaccggcggcgtcggcggcggttccgtcggtgcggtgtgcggaaggtaggcatattgaaacggcgccgtcgaccgaacgcccgggcgttacgacgggacgtggtggtctcgctcggccaggtgcagtcggggcggaggagApaIEcoNISacIIPspOMITP106YA108IP110QF112PF114NH116VA118AA120AA122AK124AA126TP128HA130FH132PY134NF136AA138AA140GL142RA144RN146AA148LH150HQ152SE154PV156HV158SP160AS162S7213-8034Runtrunt7,5407,5607,5807,6007,6207,6407,6607,6807,700
    caggccctccagctcctcgcccacccagcaacacgtcctgctgaagctaaacacctccatcgagacgagctccatccacgagcagtccgccagtgacggcgactccgacgacgagcagatcgacgtggtcaaatcggagttcgacctggacaagagcctggatgtggcgccacttgtccgggaggtcgaggagcgggtgggtcgttgtgcaggacgacttcgatttgtggaggtagctctgctcgaggtaggtgctcgtcaggcggtcactgccgctgaggctgctgctcgtctagctgcaccagtttagcctcaagctggacctgttctcggacctacaccgcggtgaaBplIBplIPflFITth111IR164PS166SS168SP170TQ172QH174VL176LK178LN180TS182IE184TS186SI188HE190QS192AS194DG196DS198DD200EQ202ID204VV206KS208EF210DL212DK214SL216DV218AP220L7213-8034Runtrunt7,7207,7407,7607,7807,8007,8207,8407,860
    cgcatgcgctgcgacctgaaggcgccctcggccatgaagccgctctaccacgagagcggtcctggagcggtagccaacagccgccagccgtcgccggaaacgaccaccaagatcaagagcgccgccgtgcagcagaagaccgtgtggcggccctactagggcatctgatccccaagcgtacgcgacgctggacttccgcgggagccggtacttcggcgagatggtgctctcgccaggacctcgccatcggttgtcggcggtcggcagcggcctttgctggtggttctagttctcgcggcggcacgtcgtcttctggcacaccgccgggatgatcccgtagactaggggttBarIBbsIR222MR224CD226LK228AP230SA232MK234PL236YH238ES240GP242GA244VA246NS248RQ250PS252PE254TT256TK258IK260SA262AV264QQ266KT268VW270RP272Y*2747213-8034runtRuntgRNA-Runt-C-2gRNA-Runt-C-1gRNA-Runt-C-37,8807,9007,9207,9407,9607,9808,0008,0208,040
    aaatctggaggaatgaagaaaacaaagtgatataacagcggcgacgcagagcggcaagcgggcaaaaattataattgtgatatagtgttataagttacgacgcctccggccggcaaacggagcagcctcatcagtgggatagaaggataaacggcagcgggagcagcagcatcaatttagacctccttacttcttttgtttcactatattgtcgccgctgcgtctcgccgttcgcccgtttttaatattaacactatatcacaatattcaatgctgcggaggccggccgtttgcctcgtcggagtagtcaccctatcttcctatttgccgtcgccctcgtcgtcgtagttrunt8,0608,0808,1008,1208,1408,1608,1808,2008,220
    ctagctgagcaaaagttatggatagaggataatggataatggatataggacaaaggaccccaatcctggaaatcagactccggcgaagttttatgctcggactcataaaatcgtgcgacgagtttgaatcacaggccctcgattttcaccaggattttttacaaatcccagcagagatcgactcgttttcaatacctatctcctattacctattacctatatcctgtttcctggggttaggacctttagtctgaggccgcttcaaaatacgagcctgagtattttagcacgctgctcaaacttagtgtccgggagctaaaagtggtcctaaaaaatgtttagggtcgtctrunt8,2408,2608,2808,3008,3208,3408,3608,3808,400
    aaacacgaaaactcaaaaactcagcccaaaaagaaaataccaagaaagcaaactttagttcaatttcatttcaacacaaaacaacaacaacacaatttgtacatagctaactagttgtaacactcataactttttttttttgagaacctatttttttcgatggataatatgcgaatttgtgcttttgagtttttgagtcgggtttttcttttatggttctttcgtttgaaatcaagttaaagtaaagttgtgttttgttgttgttgtgttaaacatgtatcgattgatcaacattgtgagtattgaaaaaaaaaaactcttggataaaaaaagctacctattatacgcttrunt8,4208,4408,4608,4808,5008,5208,5408,560
    tttagctatttttaatcattatgtttaactagtcgtctaagcgagaaatcaatttttttgtctagccataagttttagcgcgaaaagagatctaacacaaaaatcgaatttgaaacaaaaccaaataaaaaacaaaaatcacacacaaaaagtatttacaaaaatatgaagtattaaatcgataaaaattagtaatacaaattgatcagcagattcgctctttagttaaaaaaacagatcggtattcaaaatcgcgcttttctctagattgtgtttttagcttaaactttgttttggtttattttttgtttttagtgtgtgtttttcataaatgtttttatacttcataarunt3' downstre...m FlyBase)8,5808,6008,6208,6408,6608,6808,7008,7208,740
    tgttttcatttctcaaaaggttttcccgatacttttaggaaatccctttcagcttaactacgaaacatttgaaaactatccaatccctgaatcaaaatgaaaagccattttttcttttaattaaagtttccataatatttcttagattctaactttttaggattccaccttaatgacaaaagtaaagagttttccaaaagggctatgaaaatcctttagggaaagtcgaattgatgctttgtaaacttttgataggttagggacttagttttacttttcggtaaaaaagaaaattaatttcaaaggtattataaagaatctaagattgaaaaatcctaaggtggaattacPacI3' downstream region (from FlyBase)8,7608,7808,8008,8208,8408,8608,8808,9008,920
    aactaaaacgcctattttaatttggatttaccctttcaaaaaatgaacttcctatcacagttggccagcaggccagcagaaatcgaaaattaaatgattataaaatgcgaccgtgaattgcctcacatactgagaatctaaataaaattatcagaccaaaacaattggaaaacccttgattttgcggataaaattaaacctaaatgggaaagttttttacttgaaggatagtgtcaaccggtcgtccggtcgtctttagcttttaatttactaatattttacgctggcacttaacggagtgtatgactcttagatttattttaatagtctggttttgttaaccttttggg3' downstream region (from FlyBase)8,9408,9608,9809,0009,0209,0409,0609,0809,100
    aaccgcgaattaatattttcattttaacttatctccgagcattagcgataacaactatttgtggttcttagctgggggggaaatccaagtgtctggcactttcatcaaataatctcttttctaatcaatcaaaacaatcgttgagtaaccccaacgattcgatttagaatagcttttggcgcttaattataaaagtaaaattgaatagaggctcgtaatcgctattgttgataaacaccaagaatcgaccccccctttaggttcacagaccgtgaaagtagtttattagagaaaagattagttagttttgttagcaactcattggggttgctaagctaaatcttatcgaa3' downstream region (from FlyBase)9,1209,1409,1609,1809,2009,2209,2409,260
    atcacgatcgatgggatctgattttacttaggttttcccattaatttagtactcaggtattttgatattttgaagaaccatctagttttgagttggttagatacaaatgcgaacagagatataagcaacaggaaatattgtatccataatattttctgctgacattggaccagtgtagtgctagctaccctagactaaaatgaatccaaaagggtaattaaatcatgagtccataaaactataaaacttcttggtagatcaaaactcaaccaatctatgtttacgcttgtctctatattcgttgtcctttataacataggtattataaaagacgactgtaacctggtcac3' downstream region (from FlyBase)9,2809,3009,3209,3409,3609,3809,4009,4209,440
    attcttgctcatacagagagaaatcgtagtgattttttaaaaccctgaaatattttaacaatctgtccgctggtggtcctcgaaaattgaaggacatctgctgaccgcaggacatggacatggccaaagcccaaattgatggcaattaatgcggattattggtgattagggaacataagaacgagtatgtctctctttagcatcactaaaaaattttgggactttataaaattgttagacaggcgaccaccaggagcttttaacttcctgtagacgactggcgtcctgtacctgtaccggtttcgggtttaactaccgttaattacgcctaataaccactaatcccttgt3' downstream region (from FlyBase)9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tcgttgagtcggcaggagctcgcttttcgacaattgccgcattaattggcataactaactcgatttcgtagccaggactcaaatgcagcatgcagatacacagatgccaatgtaggggaaaggacattaaggatacgctgaatgcagcacatgcagttggccaaccgcagtgaaaagcaactcagccgtcctcgagcgaaaagctgttaacggcgtaattaaccgtattgattgagctaaagcatcggtcctgagtttacgtcgtacgtctatgtgtctacggttacatcccctttcctgtaattcctatgcgacttacgtcgtgtacgtcaaccggttggcgtcacttt3' downstream region (from FlyBase)9,6409,6609,6809,7009,7209,7409,7609,7809,800
    ttggtcggggccattaaccgcacagtcgcgttgagttatctccttttgctggtcaacaccttggtccatttggtttgtgtgggccgttcaaggtcgtacgctgccgcccgaccttgccagcgattggtcaaggtcgcaggtcctcgatttggtgacagaacgatggggtttctacaaccagccccggtaattggcgtgtcagcgcaactcaatagaggaaaacgaccagttgtggaaccaggtaaaccaaacacacccggcaagttccagcatgcgacggcgggctggaacggtcgctaaccagttccagcgtccaggagctaaaccactgtcttgctaccccaaagatgBsiWI3' downstream region (from FlyBase)9,8209,8409,8609,8809,9009,9209,9409,960
    cttggccaaatcgcagtgggtaagctgtcagagggaaactatttgtaagacaactccatcttgacacaagttttttttttttgtccaaaacttaaatggtgactacaagcggttgagaataaatgagaaacataggctcgattggatgataaaataaaataacacaacaagttttgaaccggtttagcgtcacccattcgacagtctccctttgataaacattctgttgaggtagaactgtgttcaaaaaaaaaaaacaggttttgaatttaccactgatgttcgccaactcttatttactctttgtatccgagctaacctactattttattttattgtgttgttcaaaa3' downstream region (from FlyBase)9,98010,00010,02010,04010,06010,08010,10010,12010,140
    aagacaatcaacatacttattctgttagttgtatgaat3' dow...Base)10,160
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    Editing disabled on read-only sequence.
    Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette (10169 bp)AclIAfeIAfeIAhdIAhdIAleIAleIAloIApaIApaIAscIBaeIBarIBbsIBbsIBmtIBmtIBplIBplIBsaIDrdIDrdIEarIEarIFspIFspIHpaIHpaIKpnIMreINcoINcoINheINheINruINruIPacIPacIPasIPciIPmlIPstIPstISmaISmaITliITliIXbaIXhoIXhoIXmaIXmaIXmnIXmnIZraIAatIIAflIIAsiSIAsiSIBamHIBamHIBbvCIBpuEIBsiWIBsiWICspCIEcoNIEcoRVEcoRVFspAIPflFIPspXIPspXISacIISacIISexAISexAISgrAISgrDITspMITspMIAcc65IBpu10IDraIIIPaeR7IPaeR7IPspOMIPspOMIHindIIIHindIIITth111I1,0002,0003,0004,0005,0006,0007,0008,0009,00010,000457...56609...927213-8034runtrunt3' downst...FlyBase)RuntN-LHRuntN-RHRuntRuntdsRed2Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette (10169 bp)

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    10169