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Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette
  • Sequence Map
    Editing disabled on read-only sequence.
    ggttaccccagcccctcgagcgtaggtgcctctctttcgttcgcctggtgccgtctctttctgcgagggggagggatcttgcgcacgtatatgaataattcaactgatcgccgtggtgattgggggatgaacggagtactggtttgtccgttcctcggaggtgcggaatgcacagatcccaatggggtcggggagctcgcatccacggagagaaagcaagcggaccacggcagagaaagacgctccccctccctagaacgcgtgcatatacttattaagttgactagcggcaccactaaccccctacttgcctcatgaccaaacaggcaaggagcctccacgccttacgtgtctagFspITliIXhoIPspXIPaeR7Irunt20406080100120140160
    gtagttctgatacccattcatttcgaggaaatttattcaggaacgtcacggtatttgcataagtgaaatcgtatctcacagttagcagcctgtatcgtagattgattactaaaatacttttctaaataatctgcactaagatatagttcagattgcgtaagatcggtaagtacagaagcatcaagactatgggtaagtaaagctcctttaaataagtccttgcagtgccataaacgtattcactttagcatagagtgtcaatcgtcggacatagcatctaactaatgattttatgaaaagatttattagacgtgattctatatcaagtctaacgcattctagccattcatgtcttcHindIIIrunt180200220240260280300320340
    cttttaatcgcactggaagttttatttaccgctcacgacatttgcatagatgaaaccgtatcttacagattcaagtagcttgcgtactaaactactgtacaaaaaatatctgcactaagaaatagtcgggagtgattttttatggtgaagtacagaagtaaattcagctaattaagccgaaaattagcgtgaccttcaaaataaatggcgagtgctgtaaacgtatctactttggcatagaatgtctaagttcatcgaacgcatgatttgatgacatgttttttatagacgtgattctttatcagccctcactaaaaaataccacttcatgtcttcatttaagtcgattaattcgg360380400420440460480500520
    tcgctcttttttgttttaaattaactgctcaacagcagtgagaaaaacattgcatatattgagtacatgaatacaggttactgtcgccttaatctcccatcggttaatcccgctaaaaagcgaagtcctttgagtttttggtggccaggtaggcactttccgtatcagatgctcgttgagcgagaaaaaacaaaatttaattgacgagttgtcgtcactctttttgtaacgtatataactcatgtacttatgtccaatgacagcggaattagagggtagccaattagggcgatttttcgcttcaggaaactcaaaaaccaccggtccatccgtgaaaggcatagtctacgagcaac540560580600620640660680700
    cttattttttgggaacatattttatggcccgtggcggcggctaatcggccaaaatatttgcggggcgtgctgcttaatccgggcgattgactttcataagcaaaggattaagattgcgcggttggactacctgttttgaggtgcgatatcagtacatgcgatggtacatctgagggccgaataaaaaacccttgtataaaataccgggcaccgccgccgattagccggttttataaacgccccgcacgacgaattaggcccgctaactgaaagtattcgtttcctaattctaacgcgccaacctgatggacaaaactccacgctatagtcatgtacgctaccatgtagactcccggEcoRV720740760780800820840860880
    aggtacgtcaaagccagtaaacccatagttttcccacttttttggggccgcaaaaaagcatcggagggcccataaaaaaagggttgatccttttggctgctgtgggctcgtagcgagttcggtatggagatcaggtactgcctggtgctcggtgatccctatgaggcggtcctgcgggtccatgcagtttcggtcatttgggtatcaaaagggtgaaaaaaccccggcgttttttcgtagcctcccgggtatttttttcccaactaggaaaaccgacgacacccgagcatcgctcaagccatacctctagtccatgacggaccacgagccactagggatactccgccaggacgcccApaIPspOMI9009209409609801,0001,0201,0401,060
    tcctgcgatgcgcgtgctgcggcagctcctgtcgcaaattgccaaggaatcgcagcaggatccaagaagcgacgacaggagcgctgatttcccgggaaaccggcaatcgtcaatcggccatcggcaatcgcgtccttgtcgcacgcccgctaaacctgcgctgtcctgccatatatccaggacgctacgcgcacgacgccgtcgaggacagcgtttaacggttccttagcgtcgtcctaggttcttcgctgctgtcctcgcgactaaagggccctttggccgttagcagttagccggtagccgttagcgcaggaacagcgtgcgggcgatttggacgcgacaggacggtatataggAfeISmaIXmaIXmaIBamHITspMITspMI1,0801,1001,1201,1401,1601,1801,2001,2201,240
    cggggctatatggtgtgaaatcggtgtagggacacgaggtccttcgcagcgagcggccgcgcacgtacaaaaggcagcgctgccgatacactggatttactggaagtgtgcgtctataatgtccagctgagagcggcgcgttctcgcgacattttggttggtcttgcctcgtagttccgccccgatataccacactttagccacatccctgtgctccaggaagcgtcgctcgccggcgcgtgcatgttttccgtcgcgacggctatgtgacctaaatgaccttcacacgcagatattacaggtcgactctcgccgcgcaagagcgctgtaaaaccaaccagaacggagcatcaaggAfeIAhdINruISmaI1,2601,2801,3001,3201,3401,3601,3801,4001,420
    cctaccagaatggccatgctccccattccttctcctcctcctccacgcggtgcctggaagagtccgccacctcggaaaactcgcgggtcctgtgtggcctcgtttttaaggttgttttggtgggccgcaattttttggccagtcaggcacttgatttgcgttccactgcacgaaatgaggatggtcttaccggtacgaggggtaaggaagaggaggaggaggtgcgccacggaccttctcaggcggtggagccttttgagcgcccaggacacaccggagcaaaaattccaacaaaaccacccggcgttaaaaaaccggtcagtccgtgaactaaacgcaaggtgacgtgctttactEarIDraIII1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gtgttgaaggaattgagaatattactttaaattgtcaagtcaagtgtcaagtgttattcagcagctcttaagaattaatacacagttaatccattttacattaagttaattaaatcgttcatatttcgtttttgaagctaagtttcgtaatctgaattactatctatgtatgtgctaacacaacttccttaactcttataatgaaatttaacagttcagttcacagttcacaataagtcgtcgagaattcttaattatgtgtcaattaggtaaaatgtaattcaattaatttagcaagtataaagcaaaaacttcgattcaaagcattagacttaatgatagatacatacacgattPacIAflII1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tcacttagccaatcgctaagcaaaatgtacaaatcttaaaacctaagtaaacgtagattagtttgaagagagttcaatgtaggcagccaaagatactcgatgttcttccaagtatgacacttccgcatcagattaaatccgatcagctagctaaacctgatccaatcaaggatcggcgagtgaatcggttagcgattcgttttacatgtttagaattttggattcatttgcatctaatcaaacttctctcaagttacatccgtcggtttctatgagctacaagaaggttcatactgtgaaggcgtagtctaatttaggctagtcgatcgatttggactaggttagttcctagccgcBmtIEarINheI1,8001,8201,8401,8601,8801,9001,9201,940
    atcgcaggcagcgaaaatggaaatgaaatcgagccggattgcgccacacgaactgagaggcgcacaaatatttacaggactaatcgcaggataatcgcccgatcagcattaggattaagttcgatccccggagagatcgatggcactgcatcttagatagtactggaagtggaccggatagcgtccgtcgcttttacctttactttagctcggcctaacgcggtgtgcttgactctccgcgtgtttataaatgtcctgattagcgtcctattagcgggctagtcgtaatcctaattcaagctaggggcctctctagctaccgtgacgtagaatctatcatgaccttcacctggcctAsiSI1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cctgacaatgggtgttagttggacagattcagatcggagattgcagatcagttgatcgagttccgagttccgagatcggcgataaagtaaattttgggcggaatcgagaaaaacattgagcaacactgagggatcagtgtgccgtaatatgatcagccggggatttctgattattttgggactgttacccacaatcaacctgtctaagtctagcctctaacgtctagtcaactagctcaaggctcaaggctctagccgctatttcatttaaaacccgccttagctctttttgtaactcgttgtgactccctagtcacacggcattatactagtcggcccctaaagactaataaaac2,1402,1602,1802,2002,2202,2402,2602,2802,300
    aatgaaacactaatttttgggacattttcgaacaatgggacgcactgttatgcacgtgtatatgtttattaattagatctgatcttaaattacttaacttaacttatttgaaatgaaattatatggataacgttgatgcatattcaaaaaaaaaaaaattaacactggaaatgattagttactttgtgattaaaaaccctgtaaaagcttgttaccctgcgtgacaatacgtgcacatatacaaataattaatctagactagaatttaatgaattgaattgaataaactttactttaatatacctattgcaactacgtataagtttttttttttttaattgtgacctttactaatcAclIPmlI2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ttttttgaacagattattaaaacgatttgtagcactaataatgatgtatttaaaatatcgtgcttatgttgatatatatattatatatatatatatatatatattattatatttattatatttttttcggcatcttcatcttaatcgataagataccttcgaaagcaattaaatatataaaaaacttgtctaataattttgctaaacatcgtgattattactacataaattttatagcacgaatacaactatatatataatatatatatatatatatatataataatataaataatataaaaaaagccgtagaagtagaattagctattctatggaagctttcgttaatttatata2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gataataaatacaaatataaaggaaaatataattcatcttatgatagtatgggatagtgtgtactatatgtactatagattattatcataattgaaaatcccatgttccaaaattttccaaactccacaatcgtatcacccatgaaattaaaaggcttaaatccttttatcccaaagtctattatttatgtttatatttccttttatattaagtagaatactatcataccctatcacacatgatatacatgatatctaataatagtattaacttttagggtacaaggttttaaaaggtttgaggtgttagcatagtgggtactttaattttccgaatttaggaaaatagggtttcaBaeI2,6802,7002,7202,7402,7602,7802,8002,8202,840
    catagtgatatagctctcctgatgcttgtgataatatttatgataatttcgcaacctgatccctcctcgcactgactaattgagtcctcatgtcctgtcggcaggacgcccctggcatcctagctactcgggaaagggaaggcaagggaacgagaaggagatggtgaagggaacccccgtatcactatatcgagaggactacgaacactattataaatactattaaagcgttggactagggaggagcgtgactgattaactcaggagtacaggacagccgtcctgcggggaccgtaggatcgatgagccctttcccttccgttcccttgctcttcctctaccacttcccttgggggRuntN-LH2,8602,8802,9002,9202,9402,9602,9803,0003,020
    cgtttggccatgggtgcctacctgtaccacccgcccccgcccccctcgacgaacccctccgcaggagggccgccaaaacacgcgcgccgctggaagtggaaaatgggaaactgttgcacggccgtttttcttttggcgtgcgtggcgaaaaaataaaattcaaagcgaagaaaaggaagcaaaccggtacccacggatggacatggtgggcgggggcggggggagctgcttggggaggcgtcctcccggcggttttgtgcgcgcggcgaccttcaccttttaccctttgacaacgtgccggcaaaaagaaaaccgcacgcaccgcttttttattttaagtttcgcttcttttccttNcoIRuntN-LH3,0403,0603,0803,1003,1203,1403,1603,1803,200
    aacgcacacaatttccagaccgcccgtggggaaaagcgagagaggaagcgagagccagagcgagagagtggaaaagtctcggtgtatttcttttttgagtttttctttggtgcaatttccaggccatcactctctctctctctttctcgctctcgcccatgcgattttccttttttccttgcgtgtgttaaaggtctggcgggcaccccttttcgctctctccttcgctctcggtctcgctctctcaccttttcagagccacataaagaaaaaactcaaaaagaaaccacgttaaaggtccggtagtgagagagagagagaaagagcgagagcgggtacgctaaaaggaaaaaaggRuntN-LH3,2203,2403,2603,2803,3003,3203,3403,3603,380
    gctgttattttttcctttttggccacaagtaacaagcgacttctctctcacgtacacaggcacactaacacgacagccaaacacacacacacacacaaacacacagagagaccgcaatggaaaaatgggaaattttttgctgtggttgttgttgcggcggcaatggtggttgctcgatcgacaataaaaaaggaaaaaccggtgttcattgttcgctgaagagagagtgcatgtgtccgtgtgattgtgctgtcggtttgtgtgtgtgtgtgtgtttgtgtgtctctctggcgttacctttttaccctttaaaaaacgacaccaacaacaacgccgccgttaccaccaacgagctaBsaIRuntN-LHrunt3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ataccgatatatacctgccccggcacccttaaaggcgcgcctgtgtgcgtaggtagccaaccacaccaccataccgcccaggataatgggcgggaaagagacggcgctagcgccgtgcaagtgggacggggcacaaacgccggcagagccgtcgacagaggcggcgagcagcgcattctatggctatatatggacggggccgtgggaatttccgcgcggacacacgcatccatcggttggtgtggtggtatggcgggtcctattacccgccctttctctgccgcgatcgcggcacgttcaccctgccccgtgtttgcggccgtctcggcagctgtctccgccgctcgtcgcgtaagAscIBmtINheIRuntN-LHrunt3,5803,6003,6203,6403,6603,6803,7003,720
    cactttaaatactgccaacgatcgcagcagagaatcagttcactcagcgactttagcgtgtcgagaacgcacagagaaatatatcgttttcgactttggaaaacagaaacaggctcgaaagaagcagagttcgaaatcgcgaaacaatccaataacaacaaacaaacgaattttttccgtgaaatttatgacggttgctagcgtcgtctcttagtcaagtgagtcgctgaaatcgcacagctcttgcgtgtctctttatatagcaaaagctgaaaccttttgtctttgtccgagctttcttcgtctcaagctttagcgctttgttaggttattgttgtttgtttgcttaaaaaaggNruIXmnIXmnIRuntN-LHrunt3,7403,7603,7803,8003,8203,8403,8603,8803,900
    aagtgacccccgataaagtgaagtgcataccgagaatccgcaatagaaaaccggcaattgtcgacagccccaggattacggctacgatttccacattcggatacgagTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATttcactgggggctatttcacttcacgtatggctcttaggcgttatcttttggccgttaacagctgtcggggtcctaatgccgatgctaaaggtgtaagcctatgctcAATTGGGATCTTTCTATCAGACGCATTTTAACTGCGTACGTAAGAACTTTATAACGAGAGAGAAAGATTTAHpaIruntRuntN-LH5'transposon endgRNA-Runt-N-43,9203,9403,9603,9804,0004,0204,0404,0604,080
    AGCGCGAATCCGTCGCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGAACTATAACGACCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACGATAATTATATCGCGCTTAGGCAGCGACACGTAAATCCTGTAGAGTCAGCGGCGAACCTCGAGGGCACTCCGCACGAACAGTTACGCCATTCACAGTGACTAAAACTTGATATTGCTGGCGCACTCAGTTTTACTGCGTACTAATAGAAAATGCACTGAAAATTCTAAATTGAGTATGCTATTAATATDrdIrunt5'transposon end4,1004,1204,1404,1604,1804,2004,2204,2404,260
    TTGTTATTTCATGTTCTACTTACGTGATAACTTATTAtatatatattttcttgttatagatatctaagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataAACAATAAAGTACAAGATGAATGCACTATTGAATAATatatatataaaagaacaatatctatagattctatgtaactactcaaacctgtttggtgttgatcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgacgttatEcoRVruntSV40 polyA5'transposon end4,2804,3004,3204,3404,3604,3804,4004,4204,440
    aacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatctagagtcgcggccgctacaggaacaggtggtggcggccctcggcgcgctcgtactgctccacgattgttcaattgttgttgttaacgtaagtaaaatacaaagtccaagtccccctccacaccctccaaaaaatttcgttcattttggagatgtttacaccataccgactaatactagatctcagcgccggcgatgtccttgtccaccaccgccgggagccgcgcgagcatgacgaggtgctHpaIXbaI*226LF224LH222HR220GE218AR216EY214QE212VI2104579-5256runtSV40 polyAdsRed24,4604,4804,5004,5204,5404,5604,5804,6004,620
    tggtgtagtcctcgttgtgggaggtgatgtccagcttggagtccacgtagtagtagccgggcagctgcacgggcttcttggccatgtagatggacttgaactccaccaggtagtggccgccgtccttcagcttcagggccttgtggatctcgcccttcagcacgccgtcgcgggggtaaccacatcaggagcaacaccctccactacaggtcgaacctcaggtgcatcatcatcggcccgtcgacgtgcccgaagaaccggtacatctacctgaacttgaggtggtccatcaccggcggcaggaagtcgaagtcccggaacacctagagcgggaagtcgtgcggcagcgcccccatAleISexAITY208DE206NH204ST202ID200LK198SD196VY194YY192GP190LQ188VP186KK184AM182YI180SK178FE176VL174YH172GG170DK168LK166LA164KH162IE160GK158LV156GD154RP152Y4579-5256runtdsRed24,6404,6604,6804,7004,7204,7404,7604,7804,800
    caggcgctcggtggacgcctcccagcccatagtcttcttctgcattacggggccgtcggaggggaagttcacgccgatgaacttcaccttgtagatgaaggagccgtcctggagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttggtccgcgagccacctgcggagggtcgggtatcagaagaagacgtaatgccccggcagcctccccttcaagtgcggctacttgaagtggaacatctacttcctcggcaggacctccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacAloIBbsIL150RE148TS146AE144WG142MT140KK138QM136VP134GD132SP130FN128VG126IF124KV122KY120IF118SG116DQ114LS112SD110QT108VT106VV104GG102DE100FN98MV96RE94WK924579-5256runtdsRed24,8204,8404,8604,8804,9004,9204,9404,9604,980
    aagccctcggggaaggacagcttcttgtagtcggggatgtcggcggggtgcttcacgtacaccttggagccgtactggaactggggggacaggatgtcccaggcgaagggcagggggccgcccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctttcgggagccccttcctgtcgaagaacatcagcccctacagccgccccacgaagtgcatgtggaacctcggcatgaccttgacccccctgtcctacagggtccgcttcccgtcccccggcgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggaAhdIFG90EP88FS86LK84KY82DP80ID78AP76HK74VY72VK70SG68YQ66FQ64PS62LI60DW58AF56PL54PG52GK50TV48KL46KA44TQ42TG40EY38PR36GE34GE324579-5256runtdsRed25,0005,0205,0405,0605,0805,1005,1205,1405,160
    cgccctcgatctcgaactcgtggccgttcacggagccctccatgcgcaccttgaagcgcatgaactccttgatgacgtcctcggaggaggccatggtggcgaccggcttcgagccgattgtttagcttgttcagctgcgcttgtttatttgcttagctttcgcttagcgacgtgttcagcgggagctagagcttgagcaccggcaagtgcctcgggaggtacgcgtggaacttcgcgtacttgaggaactactgcaggagcctcctccggtaccaccgctggccgaagctcggctaacaaatcgaacaagtcgacgcgaacaaataaacgaatcgaaagcgaatcgctgcacaagtFspINcoIZraIAatIIFspAIGE30IE28FE26HG24NV22SG20EM18RV16KF14RM12FE10KI8VD6ES4SA2M4579-5256runtdsRed23xP3 promoter5,1805,2005,2205,2405,2605,2805,3005,3205,340
    ctttgcttgtttgaattgaattgtcgctccgtagacgaagcgcctctatttatactccggcggtcgagggttcgaaatcgataagcttggatcctaattgaattagctctaattgaattagtctctaattgaattagatcctaaaagttttgttactttatagaagaaattttgagttgaaacgaacaaacttaacttaacagcgaggcatctgcttcgcggagataaatatgaggccgccagctcccaagctttagctattcgaacctaggattaacttaatcgagattaacttaatcagagattaacttaatctaggattttcaaaacaatgaaatatcttctttaaaactcaaBamHIHindIIIrunt3xP3 promoter3'transposon end5,3605,3805,4005,4205,4405,4605,4805,500
    tttgtttttttttaataaataaataaacataaataaattgtttgttgaatttattattagtatgtaagtgtaaataTAATAAAACTTAATATCTATTCAAATTAATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAACGTACGTCaaacaaaaaaaaattatttatttatttgtatttatttaacaaacaacttaaataataatcatacattcacatttatATTATTTTGAATTATAGATAAGTTTAATTATTTATTTGGAGCTATATGTCTGGCTATTTTGTGTACGCAGTTAAAATGCGTACTAATAGAAATTGCATGCAGBsiWIrunt3'transposon end5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ACAATATGATTATCTTTCTAGGGTTAAATGGCCCAGGTTCAGCTGGTCGAGTCCGGTGGTGCCCTGGTTCAGCCCGGTGGCAGCCTGCGCCTGTCCTGCGCCGCCAGCGGTTTCCCAGTGAACCGTTATTCGATGCGCTGGTACCGTCAGGCCCCCGGAAAGGAACGCGAGTGGGTGGTGTTATACTAATAGAAAGATCCCAATTTACCGGGTCCAAGTCGACCAGCTCAGGCCACCACGGGACCAAGTCGGGCCACCGTCGGACGCGGACAGGACGCGGCGGTCGCCAAAGGGTCACTTGGCAATAAGCTACGCGACCATGGCAGTCCGGGGGCCTTTCCTTGCGCTCACCCACCKpnIAcc65IMA2QV4QL6VE8SG10GA12LV14QP16GG18SL20RL22SC24AA26SG28FP30VN32RY34SM36RW38YR40QA42PG44KE46RE48WV505724-6074runtJB3Nanobodysame as JB103'transposon end5,7005,7205,7405,7605,7805,8005,8205,8405,860
    CCGGCATGTCCTCCGCCGGCGATCGCTCCAGCTATGAGGATAGCGTCAAGGGCCGTTTCACTATCTCCCGCGACGACGCCCGCAACACCGTCTATTTGCAGATGAACTCCCTGAAGCCCGAGGACACTGCCGTCTACTACTGCAACGTCAACGTCGGATTCGAGTACTGGGGCCAGGGGGCCGTACAGGAGGCGGCCGCTAGCGAGGTCGATACTCCTATCGCAGTTCCCGGCAAAGTGATAGAGGGCGCTGCTGCGGGCGTTGTGGCAGATAAACGTCTACTTGAGGGACTTCGGGCTCCTGTGACGGCAGATGATGACGTTGCAGTTGCAGCCTAAGCTCATGACCCCGGTCCCDrdIMreIAsiSISgrAIAG52MS54SA56GD58RS60SY62ED64SV66KG68RF70TI72SR74DD76AR78NT80VY82LQ84MN86SL88KP90ED92TA94VY96YC98NV100NV102GF104EY106WG108QG1105724-6074runtJB3Nanobody5,8805,9005,9205,9405,9605,9806,0006,0206,040
    CACTCAGGTCACTGTCTCGTCGggcggaggtggcggtggaatgcatctgccagcgggtccaacgatggtggccaacaacacacaggtcctggccgctgccgccgccgcagcagccgccgcagcggccgcggtagcccagggtcctgggccgcagcagagtagcaatgccaccaccgccGTGAGTCCAGTGACAGAGCAGCccgcctccaccgccaccttacgtagacggtcgcccaggttgctaccaccggttgttgtgtgtccaggaccggcgacggcggcggcgtcgtcggcggcgtcgccggcgccatcgggtcccaggacccggcgtcgtctcatcgttacggtggtggcggPasICspCISacIIMH2LP4AG6PT8MV10AN12NT14QV16LA18AA20AA22AA24AA26AA28AA30VA32QG34PG36PQ38QS40SN42AT44TA466093-6792TQ112VT114VS116S5724-6074G1G2G3G4G5G66075-6092runtRuntRuntN-RHJB3NanobodygRNA-Runt-N-2gRNA-Runt-N-1gRNA-Ru...3(new)6XGly linker6,0606,0806,1006,1206,1406,1606,1806,2006,220
    tccgcaatcgccatcaatcccgcccagagtctggccaacacgagcacccactcggccagcagcacgggcagctccacaccagatctcagcacgaacaacaccagctcgagcagcaacgccaccaccagtccgcagaactccgccaagatgcccagctcgatgaccgacatgttcgccaaggcgttagcggtagttagggcgggtctcagaccggttgtgctcgtgggtgagccggtcgtcgtgcccgtcgaggtgtggtctagagtcgtgcttgttgtggtcgagctcgtcgttgcggtggtggtcaggcgtcttgaggcggttctacgggtcgagctactggctgtacaagcggtPciITliIXhoIPspXIPaeR7ISA48IA50IN52PA54QS56LA58NT60ST62HS64AS66ST68GS70ST72PD74LS76TN78NT80SS82SS84NA86TT88SP90QN92SA94KM96PS98SM100TD102MF104A6093-6792runtRuntRuntN-RH6,2406,2606,2806,3006,3206,3406,3606,3806,400
    gcctgcacgagatgctgcaggagtatcacggcgagctggcccagaccggatcgccctcgatcctctgcagcgccctgcccaatcactggcggtcgaacaagtcgctgcccggagccttcaaggtgatcgccctggacgacgtgcccgatggcaccctggtgtccatcaagtgcggcaacggacgtgctctacgacgtcctcatagtgccgctcgaccgggtctggcctagcgggagctaggagacgtcgcgggacgggttagtgaccgccagcttgttcagcgacgggcctcggaagttccactagcgggacctgctgcacgggctaccgtgggaccacaggtagttcacgccgttAleIPstIPstIS106LH108EM110LQ112EY114HG116EL118AQ120TG122SP124SI126LC128SA130LP132NH134WR136SN138KS140LP142GA144FK146VI148AL150DD152VP154DG156TL158VS160IK162CG164N6093-6792runtRuntRuntN-RH6,4206,4406,4606,4806,5006,5206,5406,5606,580
    cgacgaaaactactgcggcgagctgaggaactgcaccacgaccatgaagaaccaggtggccaagttcaacgatctgcgcttcgtgggacgatcgggacgcggcaagtccttcacgctgaccatcaccattgccacctatccggtgcagatcgccagctacagcaaagccatcaaggtggctgcttttgatgacgccgctcgactccttgacgtggtgctggtacttcttggtccaccggttcaagttgctagacgcgaagcaccctgctagccctgcgccgttcaggaagtgcgactggtagtggtaacggtggataggccacgtctagcggtcgatgtcgtttcggtagttccacBbvCISexAIBpu10ID166EN168YC170GE172LR174NC176TT178TM180KN182QV184AK186FN188DL190RF192VG194RS196GR198GK200SF202TL204TI206TI208AT210YP212VQ214IA216SY218SK220AI222KV2246093-6792runtRuntRuntN-RH6,6006,6206,6406,6606,6806,7006,7206,7406,760
    accgtcgacgggccacgggagccaagaagtaagcaaagtgagttttttagtttccattatgttttcagtttaatccactcccaaagatccccaaaaaaccaattcagaaaccgtttgcatatcttcaattacaatattcatagttttgaaaacatttgccaacactaactagttatgatggcagctgcccggtgccctcggttcttcattcgtttcactcaaaaaatcaaaggtaatacaaaagtcaaattaggtgagggtttctaggggttttttggttaagtctttggcaaacgtatagaagttaatgttataagtatcaaaacttttgtaaacggttgtgattgatcaatactSgrDITV226DG228PR230EP232R6093-6792RuntN-RHruntRunt6,7806,8006,8206,8406,8606,8806,9006,9206,940
    tcttaaataactaatgccatttttaagctcaactttaaatgatcagtctgttttgaaatgattgcattgatcactttatcttaaagattttatcattatttttgcttatccttttgcaattcggttttgatacacatatccaaggatcttgagatccattgccgccaaccaaatccccagaatttattgattacggtaaaaattcgagttgaaatttactagtcagacaaaactttactaacgtaactagtgaaatagaatttctaaaatagtaataaaaacgaataggaaaacgttaagccaaaactatgtgtataggttcctagaactctaggtaacggcggttggtttaggggBpuEIRuntN-RH6,9606,9807,0007,0207,0407,0607,0807,1007,120
    caccatccccccaacctttttaaaaacgcattccgatgaaatacgagttgtgctttactgacttcccgccctttttctttatccttccaggctatggctatccccatcccggcgcgtttaacccgttcatgctgaatcccgcttggctggatgcggcgtacatgacctacggctatgcgtggtaggggggttggaaaaatttttgcgtaaggctactttatgctcaacacgaaatgactgaagggcgggaaaaagaaataggaaggtccgataccgataggggtagggccgcgcaaattgggcaagtacgacttagggcgaaccgacctacgccgcatgtactggatgccgatacgYG2YP4HP6GA8FN10PF12ML14NP16AW18LD20AA22YM24TY26GY28A7213-8034Runtrunt7,1407,1607,1807,2007,2207,2407,2607,280
    ggactacttccgccaccaggcagccgcccaggcggcccaggtgcatcatccggcactggccaagtcctcggcttcctcggtgtcgccaaatcccaatccatcggtggccaccagctcctcgtcggcggttcagccatctgagtatccccatccggcggcagcagtggcagcggcggcgcctgatgaaggcggtggtccgtcggcgggtccgccgggtccacgtagtaggccgtgaccggttcaggagccgaaggagccacagcggtttagggttaggtagccaccggtggtcgaggagcagccgccaagtcggtagactcataggggtaggccgccgtcgtcaccgtcgccgccgcD30YF32RH34QA36AA38QA40AQ42VH44HP46AL48AK50SS52AS54SV56SP58NP60NP62SV64AT66SS68SS70AV72QP74SE76YP78HP80AA82AV84AA86AA887213-8034Runtrunt7,3007,3207,3407,3607,3807,4007,4207,4407,460
    ggacaaccggcggccatgatgccatcgccaccgggagcggcgcctgccacgccctatgccattccgcagttcccattcaaccatgtggccgccgcagccgccgccaaggcagccacgccacacgccttccatccgtataactttgccgcggcagctggcttgcgggcccgcaatgctgcctgttggccgccggtactacggtagcggtggccctcgccgcggacggtgcgggatacggtaaggcgtcaagggtaagttggtacaccggcggcgtcggcggcggttccgtcggtgcggtgtgcggaaggtaggcatattgaaacggcgccgtcgaccgaacgcccgggcgttacgacApaISacIIPspOMIGQ90PA92AM94MP96SP98PG100AA102PA104TP106YA108IP110QF112PF114NH116VA118AA120AA122AK124AA126TP128HA130FH132PY134NF136AA138AA140GL142RA144RN146A7213-8034Runtrunt7,4807,5007,5207,5407,5607,5807,6007,6207,640
    ccctgcaccaccagagcgagccggtccacgtcagccccgcctcctccaggccctccagctcctcgcccacccagcaacacgtcctgctgaagctaaacacctccatcgagacgagctccatccacgagcagtccgccagtgacggcgactccgacgacgagcagatcgacgtggtcaagggacgtggtggtctcgctcggccaggtgcagtcggggcggaggaggtccgggaggtcgaggagcgggtgggtcgttgtgcaggacgacttcgatttgtggaggtagctctgctcgaggtaggtgctcgtcaggcggtcactgccgctgaggctgctgctcgtctagctgcaccagttBplIBplIEcoNIPflFITth111IA148LH150HQ152SE154PV156HV158SP160AS162SR164PS166SS168SP170TQ172QH174VL176LK178LN180TS182IE184TS186SI188HE190QS192AS194DG196DS198DD200EQ202ID204VV206K7213-8034Runtrunt7,6607,6807,7007,7207,7407,7607,7807,8007,820
    atcggagttcgacctggacaagagcctggatgtggcgccacttcgcatgcgctgcgacctgaaggcgccctcggccatgaagccgctctaccacgagagcggtcctggagcggtagccaacagccgccagccgtcgccggaaacgaccaccaagatcaagagcgccgccgtgcagcagtagcctcaagctggacctgttctcggacctacaccgcggtgaagcgtacgcgacgctggacttccgcgggagccggtacttcggcgagatggtgctctcgccaggacctcgccatcggttgtcggcggtcggcagcggcctttgctggtggttctagttctcgcggcggcacgtcgtcBarIS208EF210DL212DK214SL216DV218AP220LR222MR224CD226LK228AP230SA232MK234PL236YH238ES240GP242GA244VA246NS248RQ250PS252PE254TT256TK258IK260SA262AV264QQ2667213-8034RuntruntgRNA-Runt-C-27,8407,8607,8807,9007,9207,9407,9607,9808,000
    aagaccgtgtggcggccctactagggcatctgatccccaaaaatctggaggaatgaagaaaacaaagtgatataacagcggcgacgcagagcggcaagcgggcaaaaattataattgtgatatagtgttataagttacgacgcctccggccggcaaacggagcagcctcatcagtgggttctggcacaccgccgggatgatcccgtagactaggggtttttagacctccttacttcttttgtttcactatattgtcgccgctgcgtctcgccgttcgcccgtttttaatattaacactatatcacaatattcaatgctgcggaggccggccgtttgcctcgtcggagtagtcacccBbsIKT268VW270RP272Y*2747213-8034runtRuntgRNA-Runt-C-1gRNA-Runt-C-38,0208,0408,0608,0808,1008,1208,1408,1608,180
    atagaaggataaacggcagcgggagcagcagcatcaactagctgagcaaaagttatggatagaggataatggataatggatataggacaaaggaccccaatcctggaaatcagactccggcgaagttttatgctcggactcataaaatcgtgcgacgagtttgaatcacaggccctcgtatcttcctatttgccgtcgccctcgtcgtcgtagttgatcgactcgttttcaatacctatctcctattacctattacctatatcctgtttcctggggttaggacctttagtctgaggccgcttcaaaatacgagcctgagtattttagcacgctgctcaaacttagtgtccgggagcrunt8,2008,2208,2408,2608,2808,3008,3208,3408,360
    attttcaccaggattttttacaaatcccagcagaaaacacgaaaactcaaaaactcagcccaaaaagaaaataccaagaaagcaaactttagttcaatttcatttcaacacaaaacaacaacaacacaatttgtacatagctaactagttgtaacactcataactttttttttttgagtaaaagtggtcctaaaaaatgtttagggtcgtcttttgtgcttttgagtttttgagtcgggtttttcttttatggttctttcgtttgaaatcaagttaaagtaaagttgtgttttgttgttgttgtgttaaacatgtatcgattgatcaacattgtgagtattgaaaaaaaaaaactcrunt8,3808,4008,4208,4408,4608,4808,5008,5208,540
    aacctatttttttcgatggataatatgcgaatttagctatttttaatcattatgtttaactagtcgtctaagcgagaaatcaatttttttgtctagccataagttttagcgcgaaaagagatctaacacaaaaatcgaatttgaaacaaaaccaaataaaaaacaaaaatcacacacattggataaaaaaagctacctattatacgcttaaatcgataaaaattagtaatacaaattgatcagcagattcgctctttagttaaaaaaacagatcggtattcaaaatcgcgcttttctctagattgtgtttttagcttaaactttgttttggtttattttttgtttttagtgtgtgtrunt8,5608,5808,6008,6208,6408,6608,6808,7008,720
    aaaagtatttacaaaaatatgaagtatttgttttcatttctcaaaaggttttcccgatacttttaggaaatccctttcagcttaactacgaaacatttgaaaactatccaatccctgaatcaaaatgaaaagccattttttcttttaattaaagtttccataatatttcttagattctttttcataaatgtttttatacttcataaacaaaagtaaagagttttccaaaagggctatgaaaatcctttagggaaagtcgaattgatgctttgtaaacttttgataggttagggacttagttttacttttcggtaaaaaagaaaattaatttcaaaggtattataaagaatctaagaPacI3' downstream region (from FlyBase)8,7408,7608,7808,8008,8208,8408,8608,8808,900
    aactttttaggattccaccttaatgaactaaaacgcctattttaatttggatttaccctttcaaaaaatgaacttcctatcacagttggccagcaggccagcagaaatcgaaaattaaatgattataaaatgcgaccgtgaattgcctcacatactgagaatctaaataaaattatcattgaaaaatcctaaggtggaattacttgattttgcggataaaattaaacctaaatgggaaagttttttacttgaaggatagtgtcaaccggtcgtccggtcgtctttagcttttaatttactaatattttacgctggcacttaacggagtgtatgactcttagatttattttaatagt3' downstream region (from FlyBase)8,9208,9408,9608,9809,0009,0209,0409,060
    gaccaaaacaattggaaaacccaaccgcgaattaatattttcattttaacttatctccgagcattagcgataacaactatttgtggttcttagctgggggggaaatccaagtgtctggcactttcatcaaataatctcttttctaatcaatcaaaacaatcgttgagtaaccccaacgctggttttgttaaccttttgggttggcgcttaattataaaagtaaaattgaatagaggctcgtaatcgctattgttgataaacaccaagaatcgaccccccctttaggttcacagaccgtgaaagtagtttattagagaaaagattagttagttttgttagcaactcattggggttgc3' downstream region (from FlyBase)9,0809,1009,1209,1409,1609,1809,2009,2209,240
    attcgatttagaatagcttatcacgatcgatgggatctgattttacttaggttttcccattaatttagtactcaggtattttgatattttgaagaaccatctagttttgagttggttagatacaaatgcgaacagagatataagcaacaggaaatattgtatccataatattttctgctaagctaaatcttatcgaatagtgctagctaccctagactaaaatgaatccaaaagggtaattaaatcatgagtccataaaactataaaacttcttggtagatcaaaactcaaccaatctatgtttacgcttgtctctatattcgttgtcctttataacataggtattataaaagacg3' downstream region (from FlyBase)9,2609,2809,3009,3209,3409,3609,3809,4009,420
    tgacattggaccagtgattcttgctcatacagagagaaatcgtagtgattttttaaaaccctgaaatattttaacaatctgtccgctggtggtcctcgaaaattgaaggacatctgctgaccgcaggacatggacatggccaaagcccaaattgatggcaattaatgcggattattggactgtaacctggtcactaagaacgagtatgtctctctttagcatcactaaaaaattttgggactttataaaattgttagacaggcgaccaccaggagcttttaacttcctgtagacgactggcgtcctgtacctgtaccggtttcgggtttaactaccgttaattacgcctaataacc3' downstream region (from FlyBase)9,4409,4609,4809,5009,5209,5409,5609,5809,600
    tgattagggaacatcgttgagtcggcaggagctcgcttttcgacaattgccgcattaattggcataactaactcgatttcgtagccaggactcaaatgcagcatgcagatacacagatgccaatgtaggggaaaggacattaaggatacgctgaatgcagcacatgcagttggccaacactaatcccttgtagcaactcagccgtcctcgagcgaaaagctgttaacggcgtaattaaccgtattgattgagctaaagcatcggtcctgagtttacgtcgtacgtctatgtgtctacggttacatcccctttcctgtaattcctatgcgacttacgtcgtgtacgtcaaccggttg3' downstream region (from FlyBase)9,6209,6409,6609,6809,7009,7209,7409,7609,780
    cgcagtgaaattggtcggggccattaaccgcacagtcgcgttgagttatctccttttgctggtcaacaccttggtccatttggtttgtgtgggccgttcaaggtcgtacgctgccgcccgaccttgccagcgattggtcaaggtcgcaggtcctcgatttggtgacagaacgatgggggcgtcactttaaccagccccggtaattggcgtgtcagcgcaactcaatagaggaaaacgaccagttgtggaaccaggtaaaccaaacacacccggcaagttccagcatgcgacggcgggctggaacggtcgctaaccagttccagcgtccaggagctaaaccactgtcttgctaccccBsiWI3' downstream region (from FlyBase)9,8009,8209,8409,8609,8809,9009,9209,9409,960
    tttctaccttggccaaatcgcagtgggtaagctgtcagagggaaactatttgtaagacaactccatcttgacacaagttttttttttttgtccaaaacttaaatggtgactacaagcggttgagaataaatgagaaacataggctcgattggatgataaaataaaataacacaacaagaaagatggaaccggtttagcgtcacccattcgacagtctccctttgataaacattctgttgaggtagaactgtgttcaaaaaaaaaaaacaggttttgaatttaccactgatgttcgccaactcttatttactctttgtatccgagctaacctactattttattttattgtgttgttc3' downstream region (from FlyBase)9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ttttaagacaatcaacatacttaaaaattctgttagttgtatgaat3' downs...lyBase)10,15010,160
    • Linear Map
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    • Description
    Editing disabled on read-only sequence.
    Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette (10169 bp)AclIAfeIAfeIAhdIAhdIAleIAleIAloIApaIApaIAscIBaeIBarIBbsIBbsIBmtIBmtIBplIBplIBsaIDrdIDrdIEarIEarIFspIFspIHpaIHpaIKpnIMreINcoINcoINheINheINruINruIPacIPacIPasIPciIPmlIPstIPstISmaISmaITliITliIXbaIXhoIXhoIXmaIXmaIXmnIXmnIZraIAatIIAflIIAsiSIAsiSIBamHIBamHIBbvCIBpuEIBsiWIBsiWICspCIEcoNIEcoRVEcoRVFspAIPflFIPspXIPspXISacIISacIISexAISexAISgrAISgrDITspMITspMIAcc65IBpu10IDraIIIPaeR7IPaeR7IPspOMIPspOMIHindIIIHindIIITth111I1,0002,0003,0004,0005,0006,0007,0008,0009,00010,000609...92457...567213-8034runtrunt3' downst...FlyBase)RuntN-LHRuntN-RHRuntRuntdsRed2Runt-N-term JB3 fusion-fixed 5'transposon-reversed dsRed cassette (10169 bp)

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    10169