Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
XZ089 (IVT) CMV-TO-T7-ARRB2-MCP-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHIpUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIAmp (bla)180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIAmp (bla)360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattAmp (bla)540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoter8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactBspHISgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIAlwNIBsiWIAflIIIBpu10I1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)1,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaNdeICMVCMV promoter (with TetOs)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggCspCISnaBICMVCMV promoter (with TetOs)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttaaatcatgccctggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaatttagtaBsmINotIPmeISwaIAflIISacIITEV leader UTR5IVT primer bind siteT7 insertCMV pro...TetOs)homolog2,1202,1402,1602,1802,2002,2202,2402,260
    ttcttttaaagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccatgggggagaaacccgggaccagggtcttcaagaagtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccaagaaaatttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggtaccccctctttgggccctggtcccagaagttcttcagctcgggattgacgttcgagtggcacatgaacccgttcgccctgaagcatctagtggacctgtttcacctggSmaIXmaIXmnITspMIHindIIIMG2EK4PG6TR8VF10KK12SS14PN16CK18LT20VY22LG24KR26DF28VD30HL32DK34VD362342-4312ARRB2TEV leader UTR5homolog2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctgtagatggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctatggccgtgaagacctggatgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccgacatctaccgcaccacgaacacctgggactgatggacttcctggcgtttcacaaacactgggagtggacgcggaaggcgataccggcacttctggacctacacgacccgaacaggaaggcgtttctggacaagtagcggtggatggtccggaaggggggccacgggttgggtggStuIPaqCIPV38DG40VV42LV44DP46DY48LK50DR52KV54FV56TL58TC60AF62RY64GR66ED68LD70VL72GL74SF76RK78DL80FI82AT84YQ86AF88PP90VP92NP94P2342-4312ARRB22,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccggccccccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccagaatcttccatgctccgtcacactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaaggccggggggtgggcggacgtcctggccgacgactccttcgacccggtcgtacgggtggggaagaagaagtggtatggggtcttagaaggtacgaggcagtgtgacgtcggtccgggtctcctatgtcctttccggacgccgcatctgaaactctaagctcggaagacacgatttSbfIStuIBbvCIBpu10IR96PP98TR100LQ102DR104LL106RK108LG110QH112AH114PF116FF118TI120PQ122NL124PC126SV128TL130QP132GP134ED136TG138KA140CG142VD144FE146IR148AF150CA152K2342-4312ARRB22,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcactagaagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggcccccagccttcagccgaaaccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatgagtgatcttctcttttcggtgttttccttgagacacgccgaccactaggctttccacgtcaagcggggcctctttgggccgggggtcggaagtcggctttggtgtgcggtgaaggagtacagactggccagggacgtggagctccgaagggacctgttcctcgacatgatggtacAgeIBsgIBsgIEcoNIPspXIS154LE156EK158SH160KR162NS164VR166LV168IR170KV172QF174AP176EK178PG180PQ182PS184AE186TT188RH190FL192MS194DR196SL198HL200EA202SL204DK206EL208YY210H2342-4312ARRB22,8202,8402,8602,8802,9002,9202,9402,960
    gggagcccctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtacgccgacatctgcctcttcagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgccctcggggagttacatttacaggtgcagtggttgttgaggtggttctggcagttcttctagtttcagagacactctgtcatgcggctgtagacggagaagtcgtggcgggtcatgttcacaggacaccgagttgagcttgttctactggtccatagagggtcgaggtgtaagacBmgBISexAIG212EP214LN216VN218VH220VT222NN224ST226KT228VK230KI232KV234SV236RQ238YA240DI242CL244FS246TA248QY250KC252PV254AQ256LE258QD260DQ262VS264PS266ST268FC2702342-4312ARRB22,9803,0003,0203,0403,0603,0803,1003,1203,140
    taaggtgtacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgaggacaccaacctggcttccagcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctgattccacatgtggtattggggtgacgagtcgctgttggccctcttcgccccagagcgggacctaccctttgagttcgtgctcctgtggttggaccgaaggtcgtggtagcacttcctcccacggttgttcctccacgacccttaggaccacaggatgtcccagttccacttcgacAleIBlpIBsaIBsrGIKV272YT274IT276PL278LS280DN282RE284KR286GL288AL290DG292KL294KH296ED298TN300LA302SS304TI306VK308EG310AN312KE314VL316GI318LV320SY322RV324KV326KL3282342-4312ARRB23,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtggtgtctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccccagaccccagtcagccgctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgcaccacagagctccgcccctacagagacacctcgacggaaaacaagaatacgtggggttcggggtgctggtgtagggggaggggtctggggtcagtcggcgaggcctctgtctacagggacacctgtggttggagtaacttaaactatggttgatacggtgtctactactgtaacVV330SR332GG334DV336SV338EL340PF342VL344MH346PK348PH350DH352IP354LP356RP358QS360AA362PE364TD366VP368VD370TN372LI374EF376DT378NY380AT382DD384DI3862342-4312ARRB23,3403,3603,3803,4003,4203,4403,4603,4803,500
    tgtttgaggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggcGGTGGTAGCGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCacaaactcctgaaacgggccgaagccgacttcccctacttcctactgctgatactactagttgagacgccgCCACCATCGCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGAfeIBclIVF388ED390FA392RL394RL396KG398MK400DD402DY404DD406QL408CG410GG412SA414MA416SN418FT420QF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444A2342-4312MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPARRB2NESPCRhomolog3,5203,5403,5603,5803,6003,6203,6403,660
    AGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGXcmIE446WI448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468QN470RK472YT474IK476VE478VP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502C2342-4312MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGAgeIAsiSIBstEIIE504LI506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528GI530YG532GG534TG536GG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560A2342-4312from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPMCP3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGBplIBplIBstXIBstZ17IP562SN564FA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV588RQ590SS592AQ594NR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP6202342-4312from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgaCTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactGAGCTCGACCATGACGTACGTGCGTTACGATCGACGGGGAAAGGGCAGGACCCATGGGGCTCABmtIKpnINheIPspXIAcc65IIF622AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646SA648IA650AN652SG654IY656*2342-4312from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPhybrid UTR3homolog4,2204,2404,2604,2804,3004,3204,3404,360
    CTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTGAGGGGGCTGGAGCCCAGGGTCCATACGAGGGTGGAGGTGGACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTAATTGGAAATCGTTATTTGCTTTCAAATTGAAhdIBlpIKflIPaqCIhybrid UTR34,3804,4004,4204,4404,4604,4804,5004,5204,540
    AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagcTTCGATATGATTGGGGTCCCAACCAGTTAAAGCACGGTCGGTGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcgApaIBclIBmtINheIPasIPmeIXcmIPflMIPspOMIhybrid UTR3c-myc epitopeBGH polyade...n sequencePolyhi...e taghomolog4,5604,5804,6004,6204,6404,6604,6804,7004,720
    catctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtcBGH polyadenylation sequence4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctcgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgaClaIBspDIPvuIIBGH pABGH...ce4,9204,9404,9604,9805,0005,0205,0405,060
    acacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatKflINaeINgoMIV5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtacttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatBarIMscIXbaIPflFITth111IHygromycinFRT5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgtcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaEcoRIDraIIIHygromycin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtNdeIAsiSIRsrIIHygromycin5,6205,6405,6605,6805,7005,7205,7405,760
    tgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgacacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgPflFIDraIIITth111IHygromycin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagaAjuISacIIHygromycin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccctagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcgggScaIHygromycin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagctgtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaNaeIPfoINgoMIVHygromycinSV40 pA6,3206,3406,3606,3806,4006,4206,4406,460
    tataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacacttBsmIPsiIBstZ17ISV40 pA6,4806,5006,5206,5406,5606,5806,6006,6206,640
    attgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagtaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctcPvuII6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggtccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccPciISapIBspQIAflIIIpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttattggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    ccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggpUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaAlwNIpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori7,5407,5607,5807,600
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7618 bp550060006500700050010001500CMV promoter (with TetOs)CMVApNK018 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),BspHI,AhdI,BsaIFspI,AclIAmp (bla),ScaI,BcgIAclIXmnIbla promoter,SspI,BspHISgrDI,AlwNIFlpInF1 (DNA),FlpInF (DNA),EKo152.4 (DNA),MfeI,Bpu10IBsiWIAflIIIMluI,SpeINdeISnaBICspCICMV-Forward Genewiz (DNA)NdeIPflFIHygromycin,Tth111I,DraIIIAjuISacIIHyg_F (DNA)ScaIPfoI,NgoMIV,NaeISV40 pA,SV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiI,BsmI,BstZ17IM13R Genewiz (DNA)PvuII,BspQI,SapIpUCori5F (DNA),AflIII,PciIpUCoriR (DNA)pUC ori,AlwNI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    7618

    Welcome to Benchling!

    L
    Luis Santiago Mille Fragoso has shared a file with you.