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pBT137-splitD 7.101 phage backbone
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cttattatcaaccggggtacatatgattgacatgctagttttacgGttaccgttcatcgattctcttgtttgctccagactctcTggcaatgacctgatagcctttgtagaTctctcaaaaatagctaccctctcTggcatgaatttatcagctagaacggttgaatatcatgttgaataatagttggccccatgtatactaactgtacgatcaaaatgcCaatggcaagtagctaagagaacaaacgaggtctgagagAccgttactggactatcggaaacatctAgagagtttttatcgatgggagagAccgtacttaaatagtcgatcttgccaacttatagtacaaBpmIClaIMslINdeISapIBglIIBspDIBspQIBsrDIBstEIIgIIgI...S20406080100120140160
    gatggtgatttgactgtctccggcctttcCcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggGcataatgtttttggtacaaccgattctaccactaaactgacagaggccggaaagGgtgggaaaacttagaaatggatgtgtaatgagtccgtaacgtaaattttatatactcccaagatttttaaaaataggaacgcaactttatttccgaagagggcgttttcataatgtcccCgtattacaaaaaccatgttggctaaBsrDIgIIgII' promoter (internal)180200220240260280300320340
    tagGtttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccGccttttcagcCcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaaatcCaaatacgagactccgaaataacgaattaaaacgattaagaaacggaacggacatactaaataacctacaattgcgatgatgataatcatcttaactacggCggaaaagtcgGgcgcggggtttacttttatatcgatttgtccaataactggtaaacgctttacatagattHpaIHincIIgII360380400420440460480500520
    tggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggaAcaattaaccagtttgatttagatgagcaagcgtcttaacccttagttgacaatgtaccttactttgaaggtctgtggcatgaaatcaacgtataaattttgtacaactcgatgtcgtggtctaagtcgttaattcgagattcggtaggcgtttttactggagaatagttttcctTgttaatPciIAflIIIgII540560580600620640660680700
    aaggtactctctaatcctgacctgttggaAtttgcttccggtctggttcgctttgaGgctcgaattaaaacgAgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtttccatgagagattaggactggacaacctTaaacgaaggccagaccaagcgaaactCcgagcttaattttgcTctataaacttcagaaagcccgaaggagaattagaaaaactacgttaggcgaaacgaagactgatattatcagtcccatttctggactaaaaactaaataccaXmnIgIIgX promoter720740760780800820840860
    cattctcgttttctgaactgtttaaagcatttgagggAAattcaatgaatatttatgacgattccgcagtattggCcgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagtaagagcaaaagacttgacaaatttcgtaaactcccTTtaagttacttataaatactgctaaggcgtcataaccGgcgataggtcagatttgtaaaatgataatgggggagaccgttttgaagaaaacgttttcggagagcgataaaaccaaaaatagcagcagaccatttgctEaeIHpy99IgIIgXgX RBS8809009209409609801,0001,0201,040
    gggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggcccaatactatcacaacgagaatgatacggagcattaaggaaaaccgcaatacatagacgtaatcaacttacaccataaggatttagagttgactacttagaaagatggacattattacaacaaggcaatcaagcaaaataattgcatctaaaaagaagggttgcaggactgaccgIIgXgV promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagTtttgttacgttgatttgggtaatgaatatccggttcttgtatattactcggtcaagaattttagcgtattccattaagtgttactaatttcaactttaatttggtagagttcgggttaaatgatgagcaagaccacaaagagcagtcccgttcggaataagtgacttactcgtcAaaacaatgcaactaaacccattacttataggccaagaacagVgV promotergIIgXgV RBS1,2401,2601,2801,3001,3201,3401,3601,3801,400
    caagattactcttgatgaaggtcagccagcctatgcgccGggGctgtacacTgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacaTaatttatcaggcgatgatgttctaatgagaactacttccagtcggtcggatacgcggCccCgacatgtgAcaagtagacaggagaaagtttcaaccagtcaagccaagggaatactaactggcagacgcggagcaaggccgattcattgtacctcgtccagcgcctaaagctgtAttaaatagtccgctactaTatIBfuAIBspMIBsrGIgVgVII1,4201,4401,4601,4801,5001,5201,5401,560
    acaaatctccgttgtactCtgtttcgcgcttggtataatAgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgcctCcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagctgtttagaggcaacatgaGacaaagcgcgaaccatattaTcgacccccagtttctactcacaaaatcacataagaaagcggagaaagcaaaatccaaccacggaGgcatcaccgtaatgcataaaatgggcaaattacctttgaaggagtactttttcagaaatcaggagtttcgTatIBsaAIBseYIBspHIBtgZISnaBIgIXgVIIgVIII promotergVIIIgVI...BS1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttGactccctAcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaagagacatcggcaacgatgggagcaaggctacgacagaaagcgacgactcccactgctagggcgttttcgccggaaaCtgagggaTgttcggagtcgctggcttatatagccaatacgcacccgctaccaacaacagtaacagccgcgttgatagccatagttcgacaaattctttBbvCIBbvCIBsiEIBtgZIEcoP15IgVIII1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttcacctcgaaagcaagTtgataaacTgatacaattaaaggctccttttggagccttttttttTggagtAgaagagcTAttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggaagtggagctttcgttcAactatttgActatgttaatttccgaggaaaacctcggaaaaaaaaAcctcaTcttctcgATaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttccSapIBspQIaph (KanR)gIII promoterterminator 1gVIII1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgAseIBstNIPflMIPspGIaph (KanR)2,1202,1402,1602,1802,2002,2202,2402,260
    atttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccataaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtAjuIBsmINsiIPvuIAsiSIBsiEIBsmBIBsrFIEsp3Iaph (KanR)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacaAvaIBsmINsiISmaIXmaIBsoBIBstNIEcoNIPspGITspMIaph (KanR)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgttttgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaClaINruIBanIIBspDIBsp1286Iaph (KanR)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaAggccaaataggccgTtcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactTccggtttatccggcAagtctaggaaggcataaatcggtcatacaagagatcacaccaagcaacaaaaacgcactcggtactcttgcttggtaactctagtacgBglISfiIBspHI*I316RG314YK312AL310IN308EL306TT304RQ302KQ300TL298WS296FS294GN292LD290H2888-3838repANOT NECESSARILY SUFFIC... - context-dependent?aph (KanR)2,8202,8402,8602,8802,9002,9202,9402,960
    ttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgaaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtagcacatcacaaaaagaatcaggcaatgcatccatccttagactacattaccaacaaccataaaacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctBsaAISnaBIK288SQ286MD284SL282FK280AE278FS276TL274QI272KA270TL268AD266HL264TK262RL260GN258RL256YS254DS252TI250TT248PI246KD244GN242MK240IQ238NN236EL234ET232VL2302888-3838repA2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaactaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttggtggttaaagtataacgacattcacaaatttagaaatgaataaccaaagttttgggtaaccaattcggaaaatttgagtaccatcaataaaagttcgtaattgtacttMslIDM228QR226DL224EV222QF220IL218TD216TP214RG212RK210DV208VL206KM204NS202YT200NL198DK196SI194PK192LV190WQ188NL186RK184FE182HY180NN178EL176ML174MF1722888-3838repA3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcagaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattattggtgagtatttaggagtatctcataaacaaaagttttctgaattgtacaaggtctaatataaaatacttaaaaaaattgaccttttctattccgttatagagaagtPciIAflIIIKF170ED168LS166IE164IN162AK160HT158KK156QT154LE152KL150LW148EY146IR144MS142YK140NE138FS136KV134HE132LN130YK128IF126KK124LQ122FL120YP118LI116EE1142888-3838repA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ctaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttagagtttcggaaattggtttcctaaggactaaaggtgtcaagagcagtagtcgagagaccaacgaaatcgattatgtggtattcgtaaaagggatgactacaagtagtagactcgcaSF112VL110EL108KE106SS104FK102AY100NT98WQ96FI94EF92GK90VL88PN86RI84EV82TR80TM78LE76RT74AK72AL70VG68YA66NE64RS62IN60MM58QA56Y2888-3838repA3,5203,5403,5603,5803,6003,6203,6403,660
    attggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattgtaaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatcacggtgtgtcgtattttaatcgaaccaaagtacgaggcaattcagtatcgctgattagcgatcaagtaaacgaaacttttgttgattaagtctgtatgtagagttaacMfeIPsiIQ54NY52TF50SV48TR46EK44RT42PN40EI38TP36NL34LA32VC30CL28IL26KT24EH22ET20LD18YR16SI14AL12EN10AK8FV6VL4ES2M2888-3838repA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgttcagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaggaaactcaacacccatagacatttaagacgatctggaaacgaccttttgaacatttaagacgatctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaMfeISpeIpSC101 ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggtcgggacacatattgagtgatgaaatcagtcaaggcgtcataatgttttcctacagcgtttgcgacaaacgaggagatgttttgtctggaattttgggatttccgaattPsiIAflIIBseRIBseYIpSC101 ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactaccatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggactcagcgacagaaaaagcactgtaagtcaagcgacgcgagtgccgagaccgtcacttacccccatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgAvaIBcgIKasINarISfoIBsaHIBsoBIHaeIIPluTIpSC101 oripartitioning locus, gyrase binding4,2204,2404,2604,2804,3004,3204,3404,360
    ccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacgatagactgaaaaacgacaagtcgtcaaggacgggagactaaaaggtcagactggtgaagcctaatagggcactgtccagtaagtctgaccgattacgtgggtcattccBmrIAlwNIBstAPIEcoP15IpSC101 oripartitioning locus, gyrase bindinghairpin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cagcggtatcatcaacaggcttacccgtcttactgtcgctcttctgtcgccatagtagttgtccgaatgggcagaatgacagcgagaagaMspA1IpSC101 ori4,5604,580
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    Editing is disabled because sequence edits have been locked.
    4595 bpSapI Golden Gate dropout cassette: ori, KmR325035003750400042502505007501000gIIrepAgX2888-3838CTT for SapI Golden Gate cloning,gII RBS,mutated vs generation 1 backbone,+16gII' promoter (internal),mutated vs generation 1 backbone,+2HincIImutated vs generation 1 backbone,mutated vs generation 1 backbone,+1AflIII,PciImutated vs generation 1 backbone,E236 silent gII,+1mutated vs generation 1 backbone,+2gX promotergX RBSG297 silent gX RBS change,mutated vs generation 2 backbone,+3Hpy99IPsiIMfeIMfeI,SpeIPsiI,BseYIBseRI,AflIIpSC101 ori,AvaI,BsoBI,BcgI,KasI,BsaHI,NarI,SfoI,HaeII,PluTIpartitioning locus, gyrase binding,hairpin,SapI,AlwNI,BstAPI,EcoP15I,BmrI,MspA1I

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    4595