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pNM1325 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    attggggcgcgccattctagattaaccctagaaagatagtctgcgtaaaattgacgcatgcattcttgaaatattgctctctctttctaaatagcgcgaatccgtcgctgtgcatttaggacatctcagtcgccgcttggagctcccgtgaggcgtgcttgtcaatgcggtaagttaaccccgcgcggtaagatctaattgggatctttctatcagacgcattttaactgcgtacgtaagaactttataacgagagagaaagatttatcgcgcttaggcagcgacacgtaaatcctgtagagtcagcggcgaacctcgagggcactccgcacgaacagttacgccattcaAscINsiISphI5'PB terminal repeat20406080100120140160
    gtcactgattttgaactataacgaccgcgtgagtcaaaatgacgcatgattatcttttacgtgacttttaagatttaactcatacgataattatattgttatttcatgttctacttacgtgataacttattatatatatattttcttgttatagatatcaactagaatgctagcacagtgactaaaacttgatattgctggcgcactcagttttactgcgtactaatagaaaatgcactgaaaattctaaattgagtatgctattaatataacaataaagtacaagatgaatgcactattgaataatatatatataaaagaacaatatctatagttgatcttacgatcgtBmtINheIEcoRV5'PB terminal repeat180200220240260280300320340
    tgggcccatctcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatacccgggtagagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtaApaISpeIPspOMICAG promoter360380400420440460480500520
    agtaacgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttccttcattgcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggaCAG promoter540560580600620640660680700
    acttggcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggtgaaccgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccNcoISnaBICAG promoter720740760780800820840860
    cggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccgccccgccccgccccgctccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggCAG promoter8809009209409609801,0001,0201,040
    ccgctccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggcccttggcgaggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaApaIPspOMICAG promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgtgcgggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggacacgccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgccccccSacIICAG promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgacgctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacgCAG promoter1,4201,4401,4601,4801,5001,5201,5401,560
    cgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttggcccgccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacMreIPaqCISgrAIBstAPICAG promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcccaaatctggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagcctcggggctgccgcagggggacggctagggtttagaccgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgcccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagaggtagaggtcggagccccgacggcgtccccctgccgaCAG promoter1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttgttgtgctgtctcatcattttggcaaagaattgggatccgaattcctcgacggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaacaacacgacagagtagtaaaaccgtttcttaaccctaggcttaaggagctTliIXhoIBamHIEcoRIPspXIPaeR7ICAG promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gggcggccgcatggcaccgaagaagaagcgtaaagtcggaatccacggagttcctgcggcaatggacaagaagtactccattgggctcgctatcggcacaaacagcgtcggttgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaatacccccgccggcgtaccgtggcttcttcttcgcatttcagccttaggtgcctcaaggacgccgttacctgttcttcatgaggtaacccgagcgatagccgtgtttgtcgcagccaacccggcagtaatgcctgctcatgttccacggctcgttttttaagtttcaagacccgttatggNotIMA2PK4KK6RK8VG10IH12GV14PA16AM18DK20KY22SI24GL26AI28GT30NS32VG34WA36VI38TD40EY42KV44PS46KK48FK50VL52GN54TTranslation 3468-9299: Region 1sp_nCas9 (D10A)NLS unitSV40 NLS2,1202,1402,1602,1802,2002,2202,2402,260
    gatcgccacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactcttctagcggtgtcgtatttcttcttggagtaaccgcgggaggacaagctgaggcccctctgccggcttcggtgcgccgagttttcttgtcgtgccgcgtctatatgggcgtctttcttagcctagacgatggacgtcctctagaaatcattactctaccgattccacctactgagaaSbfIBglIIBsmBIEsp3IBpu10ID56RH58SI60KK62NL64IG66AL68LF70DS72GE74TA76EA78TR80LK82RT84AR86RR88YT90RR92KN94RI96CY98LQ100EI102FS104NE106MA108KV110DD112STranslation 3468-9299: Region 1sp_nCas9 (D10A)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tcttccataggctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagcttgtagacagtactgataaggctgacttgcggttgatctaagaaggtatccgacctcctcaggaaaaaccacctcctcctatttttcgtgctcgcggtgggttagaaaccgttatagcacctgctccaccgcatggtacttttcatgggttggtatatagtagactccttcttcgaacatctgtcatgactattccgactgaacgccaactagatXcmIF114FH116RL118EE120SF122LV124EE126DK128KH130ER132HP134IF136GN138IV140DE142VA144YH146EK148YP150TI152YH154LR156KK158LV160DS162TD164KA166DL168RL170IY172Translation 3468-9299: Region 1sp_nCas9 (D10A)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tctcgcgctggcgcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccgatcaacgcatccggagttgacgccaaagcaatcctgagcagagcgcgaccgcgtatactagtttaaagcccctgtgaaggagtagctccccctggacttgggtctgttgtcgctacagctgtttgagaaataggttgaccaagtctgaatgttagtcgaaaagcttctcttgggctagttgcgtaggcctcaactgcggtttcgttaggactcgSalIBspEIBstBIEcoNIBpu10ILA174LA176HM178IK180FR182GH184FL186IE188GD190LN192PD194NS196DV198DK200LF202IQ204LV206QT208YN210QL212FE214EN216PI218NA220SG222VD224AK226AI228LS230Translation 3468-9299: Region 1sp_nCas9 (D10A)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gctaggctgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagcttcaactgagcaaagacacctacgcgatccgacaggtttagggccgccgagcttttggagtagcgtgtcgagggacccctcttcttcttgccggacaaaccattagaatagcgggacagtgagcccgactgggggttgaaatttagattgaagctggaccggcttctacggttcgaagttgactcgtttctgtggatgcAR232LS234KS236RR238LE240NL242IA244QL246PG248EK250KN252GL254FG256NL258IA260LS262LG264LT266PN268FK270SN272FD274LA276ED278AK280LQ282LS284KD286TY288Translation 3468-9299: Region 1sp_nCas9 (D10A)2,8202,8402,8602,8802,9002,9202,9402,960
    atgatgatctcgacaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccatactactagagctgttagacgaccgggtctagccgctggtcatgcgtctggaaaaaaaccgccgtttcttggacagtctgcggtaagacgactcactataagacgctcacttgtgcctctagtggtttcgaggcgactcgcgatcatactagttcgcgatactactcgtggtggtDD290DL292DN294LL296AQ298IG300DQ302YA304DL306FL308AA310KN312LS314DA316IL318LS320DI322LR324VN326TE328IT330KA332PL334SA336SM338IK340RY342DE344HH346QTranslation 3468-9299: Region 1sp_nCas9 (D10A)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    agacttgactttgctgaaggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagtctgaactgaaacgacttccgggaacagtctgtcgttgacggactcttcatgttcctttaaaagaagctagtcagatttttaccgatgcggcctatgtaactgccgcctcgttcggtcctccttaaaatgtttaaataattcgggtagaaccttttttacctgccgtggctcctcD348LT350LL352KA354LV356RQ358QL360PE362KY364KE366IF368FD370QS372KN374GY376AG378YI380DG382GA384SQ386EE388FY390KF392IK394PI396LE398KM400DG402TE404ETranslation 3468-9299: Region 1sp_nCas9 (D10A)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctgctggtaaagcttaacagagaagatctgttgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcgacgaccatttcgaattgtctcttctagacaacgcgtttgtcgcgtgaaagctgttaccttcgtagggggtggtctaagtggacccgcttgacgtgcgataggagtccgccgttctcctaaagatggggaaaaactttctattgtcccttttctaactcttttaggagtgtaaagBglIIBsu36IL406LV408KL410NR412ED414LL416RK418QR420TF422DN424GS426IP428HQ430IH432LG434EL436HA438IL440RR442QE444DF446YP448FL450KD452NR454EK456IE458KI460LT462FTranslation 3468-9299: Region 1sp_nCas9 (D10A)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggataccctactatgtaggccccctcgcccggggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaaaatctgcctaacgacctatgggatgatacatccgggggagcgggcccctttaaggtctaagcgcacctactgagcgtttagtcttctctggtagtgagggaccttgaagctccttcagcacctattcccccggagacgggtcaggaagtagctttcctactgattgaaactatttttagacggattgctSmaIXmaITspMISR478FA480WM482TR484KS486EE488TI490TP492WN494FE496EV498VD500KG502AS504AQ506SF508IE510RM512TN514FD516KN518LP520NE522Translation 3468-9299: Region 2R464IP466YY468VG470PL472AR474GN476Translation 3468-9299: Region 1sp_nCas9 (D10A)3,5203,5403,5603,5803,6003,6203,6403,660
    aaaggtgcttcctaaacactctctgctgtacgagtacttcacagtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttaccgtgaaacagtttccacgaaggatttgtgagagacgacatgctcatgaagtgtcaaatattgctcgagtggttccagtttatgcagtgtcttccctactctttcggtcgtaaggacagacctctcgtcttctttcgatagcacctggaggagaagttctgcttggcctttcaatggcactttgtcBarIKV524LP526KH528SL530LY532EY534FT536VY538NE540LT542KV544KY546VT548EG550MR552KP554AF556LS558GE560QK562KA564IV566DL568LF570KT572NR574KV576TV578KQ580Translation 3468-9299: Region 2sp_nCas9 (D10A)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtcctcagagtttcttctgataaagtttttctaacttacaaagctgagacaactttagtcgcctcacctcctagcgaagttgcgtagggacccttgcatagtgctagaggacttttagtaatttctgttcctgaaggacctgttactcctcttgctcctgtaagaactcctgtaacaggagtPflFITth111ILK582ED584YF586KK588IE590CF592DS594VE596IS598GV600ED602RF604NA606SL608GT610YH612DL614LK616II618KD620KD622FL624DN626EE628NE630DI632LE634DI636VL638Translation 3468-9299: Region 2sp_nCas9 (D10A)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatgggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaatcctgggaatgcaacaaacttctatccctctactaacttcttgcgaacttttgaatgcgagtagagaagctgctgtttcagtactttgtcgagttctccgcggctatatgtcctacccccgccgacagttcttttgactagttaccctaggctctgttcgtctcacctttctgttaggaBamHIPflFITth111ITL640TL642FE644DR646EM648IE650ER652LK654TY656AH658LF660DD662KV664MK666QL668KR670RR672YT674GW676GR678LS680RK682LI684NG686IR688DK690QS692GK694TI696LTranslation 3468-9299: Region 2sp_nCas9 (D10A)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggattttcttaagtccgatggatttgccaaccggaacttcatgcagttgatccatgatgactctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacagtcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgcctaaaagaattcaggctacctaaacggttggccttgaagtacgtcaactaggtactactgagagagtggaaattcctcctgtaggtctttcgtgttcaaagaccggtccccctgtcagaagtgctcgtgtagcgattagaacgtccatcgggtcgatagtttttcccttatgacAjuIAflIID698FL700KS702DG704FA706NR708NF710MQ712LI714HD716DS718LT720FK722ED724IQ726KA728QV730SG732QG734DS736LH738EH740IA742NL744AG746SP748AI750KK752GI754LTranslation 3468-9299: Region 2sp_nCas9 (D10A)4,2204,2404,2604,2804,3004,3204,3404,360
    cagaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatatcgttatcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtagggaaaggatgaagaggattgaagagggtataaaagaactggggtcccaaatccttagtctggcaattccagcacctacttgagcagtttcattacccttccgtattcgggctcttatagcaatagctctaccgggctctcttggtttgatgggtcttccctgtcttcttgtcatccctttcctacttctcctaacttctcccatattttcttgaccccagggtttaggaatKflIAflIIQ756TV758KV760VD762EL764VK766VM768GR770HK772PE774NI776VI778EM780AR782EN784QT786TQ788KG790QK792NS794RE796RM798KR800IE802EG804IK806EL808GS810QI812LTranslation 3468-9299: Region 2sp_nCas9 (D10A)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aggaacacccagttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacggcagggacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtggatcatatcgtgccccagtcttttctcaaagatgattctattgataataatccttgtgggtcaacttttgtgggtcgaagtcttactcttcgagatggacatgatggacgtcttgccgtccctgtacatgcacctagtccttgacctgtagttagccgagaggctgatgctgcacctagtatagcacggggtcagaaaagagtttctactaagataactattattPciIBmgBIK814EH816PV818EN820TQ822LQ824NE826KL828YL830YY832LQ834NG836RD838MY840VD842QE844LD846IN848RL850SD852YD854VD856HI858VP860QS862FL864KD866DS868ID870NK872Translation 3468-9299: Region 2sp_nCas9 (D10A)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agtgttgacaagatccgataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaatgaaaaattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataatctgactaaggctgaacgaggtggcctgtctgagttggataaatcacaactgttctaggctatttttatctcccttctcactattgcaggggagtcttcttcaacagttcttttactttttaataaccgccgtcgacgacttgcggtttgactagtgtgttgccttcaagctattagactgattccgacttgctccaccggacagactcaacctatttVL874TR876SD878KN880RG882KS884DN886VP888SE890EV892VK894KM896KN898YW900RQ902LL904NA906KL908IT910QR912KF914DN916LT918KA920ER922GG924LS926EL928DK930Translation 3468-9299: Region 2sp_nCas9 (D10A)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gccggcttcatcaaaaggcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcatgaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaagtctaagctggtctcagatttcagaaaggactttcagtcggccgaagtagttttccgtcgaacaactctgtgcggtctagtggttcgtgcaccgggtttaagagctaagtgcgtacttgtggttcatgctacttttactgtttgactaagctctccactttcaataatgagacttcagattcgaccagagtctaaagtctttcctgaaagtcaPmlIPsrIBstXIAG932FI934KR936QL938VE940TR942QI944TK946HV948AQ950IL952DS954RM956NT958KY960DE962ND964KL966IR968EV970KV972IT974LK976SK978LV980SD982FR984KD986FQ988Translation 3468-9299: Region 2sp_nCas9 (D10A)4,9204,9404,9604,9805,0005,0205,0405,060
    tttataaggtgagagagatcaacaattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaaaaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttaggaaaatgatcgcaaagtctgagcaggaaataggaaatattccactctctctagttgttaatggtggtacgcgtactacggatggacttacgtcaccatccgtgacgtgaatagttttttatagggttcgaacttagacttaaacaaatgcctctgatatttcacatgctacaatccttttactagcgtttcagactcgtcctttatccFspAIFY990KV992RE994IN996NY998HH1000AH1002DA1004YL1006NA1008VV1010GT1012AL1014IK1016KY1018PK1020LE1022SE1024FV1026YG1028DY1030KV1032YD1034VR1036KM1038IA1040KS1042EQ1044EI1046GTranslation 3468-9299: Region 2sp_nCas9 (D10A)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    caaggccaccgctaagtacttcttttacagcaatattatgaattttttcaagaccgagattacactggccaatggagagattcggaagcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtagggatttcgcgacagtccggaaggtcctgtccgttccggtggcgattcatgaagaaaatgtcgttataatacttaaaaaagttctggctctaatgtgaccggttacctctctaagccttcgctggtgaatagctttgtttgcctctttgtcctctttagcacaccctgttcccatccctaaagcgctgtcaggccttccaggacaggNruIBspEIPaqCIK1048AT1050AK1052YF1054FY1056SN1058IM1060NF1062FK1064TE1066IT1068LA1070NG1072EI1074RK1076RP1078LI1080ET1082NG1084ET1086GE1088IV1090WD1092KG1094RD1096FA1098TV1100RK1102VL1104STranslation 3468-9299: Region 2sp_nCas9 (D10A)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    atgccgcaggtgaacatcgttaaaaagaccgaagtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagctgatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagtcgcttacagtgtactggttgtggtacggcgtccacttgtagcaatttttctggcttcatgtctggcctccgaagaggttcctttcataggagggcttttccttgtcgctgttcgactagcgtgcgttttttctaaccctggggttctttatgccgcctaagctaagaggatgtcagcgaatgtcacatgaccaacaccKflIM1106PQ1108VN1110IV1112KK1114TE1116VQ1118TG1120GF1122SK1124ES1126IL1128PK1130RN1132SD1134KL1136IA1138RK1140KD1142WD1144PK1146KY1148GG1150FD1152SP1154TV1156AY1158SV1160LV1162VTranslation 3468-9299: Region 2sp_nCas9 (D10A)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccaaagtggagaaagggaagtctaaaaaactcaaaagcgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccatcgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcccaagtactctctctttgaggtttcacctctttcccttcagattttttgagttttcgcagttccttgacgacccgtagtgttagtacctcgctagttcgaagctttttttggggtagctgaaagagctccgctttcctatatttctccagttttttctggagtagtaattcgaagggttcatgagagagaaactTliIXhoIBstBIPaeR7IA1164KV1166EK1168GK1170SK1172KL1174KS1176VK1178EL1180LG1182IT1184IM1186ER1188SS1190FE1192KN1194PI1196DF1198LE1200AK1202GY1204KE1206VK1208KD1210LI1212IK1214LP1216KY1218SL1220FE1222Translation 3468-9299: Region 2sp_nCas9 (D10A)5,6205,6405,6605,6805,7005,7205,7405,760
    gcttgaaaacggccggaaacgaatgctcgctagtgcgggcgagctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggccagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgtggaacaacacaaacgaacttttgccggcctttgcttacgagcgatcacgcccgctcgacgtctttccattgctcgaccgtgacgggagatttatgcaattaaagaacatagaccggtcggtgatacttttcgagtttcccagagggcttctattactcgtcttcgtcgacaagcaccttgttgtgtttLE1224NG1226RK1228RM1230LA1232SA1234GE1236LQ1238KG1240NE1242LA1244LP1246SK1248YV1250NF1252LY1254LA1256SH1258YE1260KL1262KG1264SP1266ED1268NE1270QK1272QL1274FV1276EQ1278HK1280Translation 3468-9299: Region 2sp_nCas9 (D10A)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cactaccttgatgagatcatcgagcaaataagcgaattctccaaaagagtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacagggataagcccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttgggcgcgcctggtgatggaactactctagtagctcgtttattcgcttaagaggttttctcactaggagcggctgcgattggagctattccacgaaagacgaatgttattcgtgtccctattcgggtagtccctcgtccgtcttttgtaataggtgaacaaatgagactggttgaacccgcgcggacAscIBplIBplIEcoNIEcoRIHY1282LD1284EI1286IE1288QI1290SE1292FS1294KR1296VI1298LA1300DA1302NL1304DK1306VL1308SA1310YN1312KH1314RD1316KP1318IR1320EQ1322AE1324NI1326IH1328LF1330TL1332TN1334LG1336AP1338Translation 3468-9299: Region 2sp_nCas9 (D10A)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctacaaaggaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaagaatcgacctctctcagctcggtggagacagcagggctgaccccaagaagaagaggaaggtggggtcggaagttcatgaagctgtggtggtatctgtctttcgccatgtggagatgtttcctccaggacctgcggtgtgactaagtagtcagttaatgccccgagatactttgttcttagctggagagagtcgagccacctctgtcgtcccgactggggttcttcttctccttccacccAA1340FK1342YF1344DT1346TI1348DR1350KR1352YT1354ST1356KE1358VL1360DA1362TL1364IH1366QS1368IT1370GL1372YE1374TR1376ID1378LS1380QL1382GG1384DS1386RA1388DP1390KK1392KR1394KV1396GTranslation 3468-9299: Region 2sp_nCas9 (D10A)NLS6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tggaggaggtaccggcggtggaggctcagcagaatacgtacgagctctgtttgacttcaatgggaatgacgaggaggatctcccctttaagaagggcgatattctccgcatcagagataagcccgaagaacaatggtggaatgccgaggatagcgaagggaaaaggggcatgattacctcctccatggccgccacctccgagtcgtcttatgcatgctcgagacaaactgaagttacccttactgctcctcctagaggggaaattcttcccgctataagaggcgtagtctctattcgggcttcttgttaccaccttacggctcctatcgcttcccttttccccgtactaaKpnIBsiWISnaBIAcc65IG1398GG1400TG1402GG1404GS1406AE1408YV1410RA1412LF1414DF1416NG1418ND1420EE1422DL1424PF1426KK1428GD1430IL1432RI1434RD1436KP1438EE1440QW1442WN1444AE1446DS1448EG1450KR1452GM1454ITranslation 3468-9299: Region 2Mutated SH3 domain2x GS linker6,3206,3406,3606,3806,4006,4206,4406,460
    ctggtgccatatgtggagaaatattccggtgactacaaagaccatgatggggattacaaagaccacgacatcgactacaaagacgacgacgataaatcagggatgacagacgccgagtacgtgcgcattcatgagaaactggatatttacaccttcaagaagcagttcttcaacagaccacggtatacacctctttataaggccactgatgtttctggtactacccctaatgtttctggtgctgtagctgatgtttctgctgctgctatttagtccctactgtctgcggctcatgcacgcgtaagtactctttgacctataaatgtggaagttcttcgtcaagaagttgtFspAIL1456VP1458YV1460EK1462YS1464GD1466YK1468DH1470DG1472DY1474KD1476HD1478ID1480YK1482DD1484DD1486KS1488GM1490TD1492AE1494YV1496RI1498HE1500KL1502DI1504YT1506FK1508KQ1510FF1512NTranslation 3468-9299: Region 23x Flag plus linkerPmCDA1Mutated SH3 domainFLAGFLAGFLAG6,4806,5006,5206,5406,5606,5806,6006,6206,640
    acaagaaatctgtgtcacaccgctgctacgtgctgtttgagttgaagcgaaggggcgaaagaagggcttgcttttggggctatgccgtcaacaagccccaaagtggcaccgagagaggaatacacgctgagatattcagtatccgaaaggtggaagagtatcttcgggataatcctgttctttagacacagtgtggcgacgatgcacgacaaactcaacttcgcttccccgctttcttcccgaacgaaaaccccgatacggcagttgttcggggtttcaccgtggctctctccttatgtgcgactctataagtcataggctttccaccttctcatagaagccctattaggN1514KK1516SV1518SH1520RC1522YV1524LF1526EL1528KR1530RG1532ER1534RA1536CF1538WG1540YA1542VN1544KP1546QS1548GT1550ER1552GI1554HA1556EI1558FS1560IR1562KV1564EE1566YL1568RD1570NP1572Translation 3468-9299: Region 2PmCDA16,6606,6806,7006,7206,7406,7606,7806,8006,820
    tgggcagtttacgatcaactggtattccagctggagtccttgcgctgattgtgccgagaaaattctggaatggtataatcaggaacttcggggaaacgggcacacattgaaaatctgggcctgcaagctgtactacgagaagaatgcccggaaccagataggactctggaatctgacccgtcaaatgctagttgaccataaggtcgacctcaggaacgcgactaacacggctcttttaagaccttaccatattagtccttgaagcccctttgcccgtgtgtaacttttagacccggacgttcgacatgatgctcttcttacgggccttggtctatcctgagaccttagacGQ1574FT1576IN1578WY1580SS1582WS1584PC1586AD1588CA1590EK1592IL1594EW1596YN1598QE1600LR1602GN1604GH1606TL1608KI1610WA1612CK1614LY1616YE1618KN1620AR1622NQ1624IG1626LW1628NL1630Translation 3468-9299: Region 2PmCDA16,8406,8606,8806,9006,9206,9406,9606,9807,000
    agggacaatggtgtaggcctgaacgtgatggtttccgagcactatcagtgttgtcggaagattttcatccaaagctctcataaccagctcaatgaaaaccgctggttggagaaaacactgaaacgtgcggagaagtggagatccgagctgagcatcatgatccaggtcaagattctccctgttaccacatccggacttgcactaccaaaggctcgtgatagtcacaacagccttctaaaagtaggtttcgagagtattggtcgagttacttttggcgaccaacctcttttgtgactttgcacgcctcttcacctctaggctcgactcgtagtactaggtccagttctaagStuIRD1632NG1634VG1636LN1638VM1640VS1642EH1644YQ1646CC1648RK1650IF1652IQ1654SS1656HN1658QL1660NE1662NR1664WL1666EK1668TL1670KR1672AE1674KW1676RS1678EL1680SI1682MI1684QV1686KI1688Translation 3468-9299: Region 2PmCDA17,0207,0407,0607,0807,1007,1207,1407,160
    tgcataccactaagtctccagccgttggtcccaagaagaaaagaaaagtcggtaccatgaccaacctttccgacatcatagagaaggaaacaggcaaacagttggtcatccaagagtcgatactcatgcttcctgaagaagttgaggaggtcattgggaataagccggaaagtgaacgtatggtgattcagaggtcggcaaccagggttcttcttttcttttcagccatggtactggttggaaaggctgtagtatctcttcctttgtccgtttgtcaaccagtaggttctcagctatgagtacgaaggacttcttcaactcctccagtaacccttattcggcctttcactKpnIAcc65ILH1690TT1692KS1694PA1696VG1698PK1700KK1702RK1704VG1706TM1708TN1710LS1712DI1714IE1716KE1718TG1720KQ1722LV1724IQ1726ES1728IL1730ML1732PE1734EV1736EE1738VI1740GN1742KP1744ES1746DTranslation 3468-9299: Region 2UgiPmCDA1SV40 NLS7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cattctcgtacacactgcgtatgatgagagcaccgatgagaacgtgatgctgctcacgtcagatgccccagagtacaaaccctgggctctggtgattcaggactctaatggagagaacaagatcaagatgctagaaggcaggggaagccttctgacttgtggggatgtggaagaagtaagagcatgtgtgacgcatactactctcgtggctactcttgcactacgacgagtgcagtctacggggtctcatgtttgggacccgagaccactaagtcctgagattacctctcttgttctagttctacgatcttccgtccccttcggaagactgaacacccctacaccttcttBmgBII1748LV1750HT1752AY1754DE1756ST1758DE1760NV1762ML1764LT1766SD1768AP1770EY1772KP1774WA1776LV1778IQ1780DS1782NG1784EN1786KI1788KM1790LE1792GR1794GS1796LL1798TC1800GD1802VE1804ETranslation 3468-9299: Region 2UgiT2A+linker7,3607,3807,4007,4207,4407,4607,4807,5007,520
    aaccctggtccatctagaatggccaagcctttgtctcaagaagaatccaccctcattgaaagagcaacggctacaatcaacagcatccccatctctgaagactacagcgtcgccagcgcagctctctctagcgacggccgcatcttcactggtgtcaatgtatatcattttactgttgggaccaggtagatcttaccggttcggaaacagagttcttcttaggtgggagtaactttctcgttgccgatgttagttgtcgtaggggtagagacttctgatgtcgcagcggtcgcgtcgagagagatcgctgccggcgtagaagtgaccacagttacatatagtaaaatgacN1806PG1808PS1810RM1812AK1814PL1816SQ1818EE1820ST1822LI1824ER1826AT1828AT1830IN1832SI1834PI1836SE1838DY1840SV1842AS1844AA1846LS1848SD1850GR1852IF1854TG1856VN1858VY1860HF1862TTranslation 3468-9299: Region 2BlastRT2A...er7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ggggaccttgtgcagaactcgtggtgctgggcactgctgctgctgcggcagctggcaacctgacttgtatcgtcgcgatcggaaatgagaacaggggcatcttgagcccctgcggacggtgccgacaggtgcttctcgatctgcatcctgggatcaaagccatagtgaaggacagcccctggaacacgtcttgagcaccacgacccgtgacgacgacgacgccgtcgaccgttggactgaacatagcagcgctagcctttactcttgtccccgtagaactcggggacgcctgccacggctgtccacgaagagctagacgtaggaccctagtttcggtatcacttcctgtcNruIG1864GP1866CA1868EL1870VV1872LG1874TA1876AA1878AA1880AG1882NL1884TC1886IV1888AI1890GN1892EN1894RG1896IL1898SP1900CG1902RC1904RQ1906VL1908LD1910LH1912PG1914IK1916AI1918VK1920DS1922Translation 3468-9299: Region 2BlastR7,7207,7407,7607,7807,8007,8207,8407,860
    tgatggacagccgacggcagttgggattcgtgaattgctgccctctggttatgtgtgggagggctaaatcgatgatctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcactacctgtcggctgccgtcaaccctaagcacttaacgacgggagaccaatacacaccctcccgatttagctactagatctcgagcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagClaIBspDIDG1924QP1926TA1928VG1930IR1932EL1934LP1936SG1938YV1940WE1942G*1944Translation 3468-9299: Region 2BGH pABlastR7,8807,9007,9207,9407,9607,9808,0008,0208,040
    ccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaacctgcagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggacgtcSphIBGH pA8,0608,0808,1008,1208,1408,1608,1808,2008,220
    cccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacgggttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttglac promoterLacOM13-rev8,2408,2608,2808,3008,3208,3408,3608,3808,400
    ctgtcgtgccagcggatcttaaaagttttgttactttatagaagaaattttgagtttttgtttttttttaataaataaataaacataaataaattgtttgttgaatttattattagtatgtaagtgtaaatataataaaacttaatatctattcaaattaataaataaacctcgagacagcacggtcgcctagaattttcaaaacaatgaaatatcttctttaaaactcaaaaacaaaaaaaaattatttatttatttgtatttatttaacaaacaacttaaataataatcatacattcacatttatattattttgaattatagataagtttaattatttatttggagct3' PB terminal repeat8,4208,4408,4608,4808,5008,5208,5408,560
    tatacagaccgataaaacacatgcgtcaattttacgcatgattatctttaacgtacgtcacaatatgattatctttctagggttaatctagtatacgcgtttaattaatccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcatatgtctggctattttgtgtacgcagttaaaatgcgtactaatagaaattgcatgcagtgttatactaatagaaagatcccaattagatcatatgcgcaaattaattaggtcgaaaacaagggaaatcactcccaattaacgcgcgaaccgcattagtaccagtatcgacaaagMluIPacIBsiWIBstZ17I3' PB terminal repeatT3T3 promoter8,5808,6008,6208,6408,6608,6808,7008,7208,740
    ctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgc8,7608,7808,8008,8208,8408,8608,8808,9008,920
    cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaagcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttPciISapIBspQI8,9408,9608,9809,0009,0209,0409,0609,0809,100
    aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacccttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggColE1 origin9,1209,1409,1609,1809,2009,2209,2409,260
    tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgaacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactApaLIColE1 origin9,2809,3009,3209,3409,3609,3809,4009,4209,440
    gtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggacaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctColE1 origin9,4609,4809,5009,5209,5409,5609,5809,6009,620
    aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcattttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtColE1 origin9,6409,6609,6809,7009,7209,7409,7609,7809,800
    tgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcaAhdIW220HK218IL216SA214GI212EA210IQ208RN206RE204DM202TA200QS198GT196AmpR9,8209,8409,8609,8809,9009,9209,9409,960
    cgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgtgcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaTY194IV192VI190RS188PK186GD184PG182LA180AI178IG176RS174GR172EG170AG168SK166DA164IF162WG160AP158LA156SR154LL152PG150AV148KD146AE144MW142DI140LQ138AmpR9,98010,00010,02010,04010,06010,08010,10010,12010,140
    tgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaAclIQR136SA134LT132LL130EG128TL126LK124RL122TT120AM118AV116PM114TT112DR110ED108NP106IA104EN102LE100PE98WR96DL94RT92VH90DG88MN86HL84FA82TL80EAmpR10,16010,18010,20010,22010,24010,26010,28010,30010,320
    ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagK78PG76GI74TT72LL70LN68AA66TN64DS62MT60IA58AS56CL54ER52VT50MG48DT46LH44KE42TV40PS38YE36VL34DN32QS30YH28IR26RG24LQ22EAmpR10,34010,36010,38010,40010,42010,44010,46010,48010,500
    ttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggAclIApaLIQ20GA18DI16RS14LV12AG10CL8LV6KF4TS2MAmpR10,52010,54010,56010,58010,60010,62010,64010,660
    agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgttt10,68010,70010,72010,74010,76010,78010,80010,82010,840
    taggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaa10,86010,88010,90010,92010,94010,96010,98011,00011,020
    ccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcAloIDraIIIF1 ori11,04011,06011,08011,10011,12011,14011,16011,18011,200
    cttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttggaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacW23E22G21N20L19S18R17L16Q15Q14LacZ alphaF1 ori11,22011,24011,26011,28011,30011,32011,34011,360
    ggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcgcattatgctgagtgatatcccgctS13P12R11D10T9R8A7E6E5S4N3R2W1LacZ alphaT7M13-fwd11,38011,40011,42011,44011,46011,48011,50011,520
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    11534 bp8500900095001000010500110005001000150020002500SV40 NLS5'PB terminal repeat,AscI,SphI,NsiI,EcoRV,NheI,BmtI,PspOMI,ApaI,SpeISnaBI,NcoICAG promoter,PspOMI,ApaISacII,PaqCIMreI,SgrAI,BstAPIBamHI,EcoRINLS unit,PaeR7I,PspXI,TliI,XhoI,NotIBsmBI,Esp3I,SbfI,BglII,Bpu10IXcmI,EcoNISalITranslation 3468-9299: Region 1,BstBI,BspEI,Bpu10IT3,T3 promoter,BstZ17I,MluI,PacIBspQI,SapIPciIApaLIColE1 originAhdIAmpR,AclIAclI,ApaLIF1 ori,M13-fwd,T7,LacZ alpha,AloI,DraIII

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    11534