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APL0038 fly eko450 sensor - 5682-1_ActGly1APL
  • Sequence Map
    Editing disabled on read-only sequence.
    aaaaataggcgtatcacgaggccctttcgtcttcaagaattaattctatattctaaaaacacaaatgatacttctaaaaaaaaatcatgaatggcatcaactctgaatcaaatctttgcagatgcacctacttctcatttccactgtcacatcatttttccagatctcgctgccttttttatccgcatagtgctccgggaaagcagaagttcttaattaagatataagatttttgtgtttactatgaagatttttttttagtacttaccgtagttgagacttagtttagaaacgtctacgtggatgaagagtaaaggtgacagtgtagtaaaaaggtctagagcgacggaAseIBglIIAc5 promoter20406080100120140160
    gttatgtggcccacaaaccaagacacgttttatggccattaaagctggctgatcgtcgccaaacaccaaatacataatgaatatgtacacattcgagaaagaagcgatcaaagaagcgtcttcgggcggagtaggagaatgcggaggagaaggagaacgagctgatctagtatctcaatacaccgggtgtttggttctgtgcaaaataccggtaatttcgaccgactagcagcggtttgtggtttatgtattacttatacatgtgtaagctctttcttcgctagtttcttcgcagaagcccgcctcatcctcttacgcctcctcttcctcttgctcgactagatcatagaAc5 promoter180200220240260280300320340
    ctccacaatccaatgccaactgaccaactggccatattcggagcaatttgaagccaatttccatcgcctggcgatcgctccattcttggctatatgtttttcaccgttacccggggccattttcaaagactcgtcggcaagataagattgtgtcactcgctgtctctcttcatttgaggtgttaggttacggttgactggttgaccggtataagcctcgttaaacttcggttaaaggtagcggaccgctagcgaggtaagaaccgatatacaaaaagtggcaatgggccccggtaaaagtttctgagcagccgttctattctaacacagtgagcgacagagagaagtaaaSmaIXmaIAsiSIPflMITspMIAc5 promoter360380400420440460480500520
    gtcgaagaatgctgaggaatttcgcgatgacgtcggcgagtattttgaagaatgagaataatttgtatttatacgaaaatcagttagtggaattttctacaaaaacatgttatctatagataattttgttgcaaaatatgttgactatgacaaagattgtatgtatatacctttacagcttcttacgactccttaaagcgctactgcagccgctcataaaacttcttactcttattaaacataaatatgcttttagtcaatcaccttaaaagatgtttttgtacaatagatatctattaaaacaacgttttatacaactgatactgtttctaacatacatatatggaaatNruIPciIBbvCIAc5 promoter540560580600620640660680700
    atgtattctcattttcttatgtatttataatggcaatgatgatactgatgatattttaagatgatgccagaccaaaaggcttgaatttctgcgtcttttgccgaacgcagtgcatgtgcaattgttgttttttggaatattcaattttcggactgtccgctttgatttcagtttctacataagagtaaaagaatacataaatattaccgttactactatgactactataaaattctactacggtctggttttccgaacttaaagacgcagaaaacggcttgcgtcacgtacacgttaacaacaaaaaaccttataagttaaaagcctgacaggcgaaactaaagtcaaagAc5 promoter720740760780800820840860
    ttggcttattcaaaaagcaaagtaaagccaaaaaagcgagatggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcggaaggaagggtggggcggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttggagccactcctttgaccaaaccgaataagtttttcgtttcatttcggttttttcgctctaccgttatggtttacgccgttttgccatcaccttcctttccccacgccccgtcgccttccttcccaccccgccccgcaccgccccagacaccgacccgcgctgcagtggctgcaacctcggtgaggaaactggtAc5 promoter8809009209409609801,0001,0201,040
    tgtgtgcgtgtgtgtattattcgtgtctcgccactcgccggttgtttttttctttttatgctgcgctctctctagcgccatctcgcttacgcatgctcaacgcaccgcatgttgccgtttccttttatgcgtcattttggctcgaaataggcaattatttaaacaaagattagtcacacacgcacacacataataagcacagagcggtgagcggccaacaaaaaaagaaaaatacgacgcgagagagatcgcggtagagcgaatgcgtacgagttgcgtggcgtacaacggcaaaggaaaatacgcagtaaaaccgagctttatccgttaataaatttgtttctaatcagSphIAc5 promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aacgaaaacgctaaaataaataagtctacaatatggttacttattgccatgtgtgtgcagccaacgatagcaacaaaagcaacaacacaggtggctttccctctttcactttttgtttgcaagccgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaagattgcttttgcgattttatttattcagatgttataccaatgaataacggtacacacacgtcggttgctatcgttgttttcgttgttgtgtccaccgaaagggagaaagtgaaaaacaaacgttcggcgcacgctcgttctgccgtgctggccgtttgcgttaatgcgactgtttctCspCIAc5 promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gcagacgaagttttggcgaaaaacatcaaggcgcctgatacgaatgcatttgcaataacaattgcgatatttaatattgtttatgaagctgtttgacttcaaaacacacaaaaaaaaaaataaaacaaattatttgaaagagaattaggaatcggacgcttatcgttagggtaaccgtctgcttcaaaaccgctttttgtagttccgcggactatgcttacgtaaacgttattgttaacgctataaattataacaaatacttcgacaaactgaagttttgtgtgtttttttttttattttgtttaataaactttctcttaatccttagcctgcgaatagcaatcccattgNsiIAc5 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    aacaagaaatgcttactgagtcacagcctctggaaaactgccgcaagccagagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgtgaggcgtctctctcttcgtctctgttgcgcaaacgcatagactgcactgaaaaaatcgattacctattttttattgttctttacgaatgactcagtgtcggagaccttttgacggcgttcggtctctctctctctttttctccctctcgtcgaatctggcgtacacgaacacacactccgcagagagagaagcagagacaacgcgtttgcgtatctgacgtgacttttttagctaatggataaaaaatClaIFspIBspDIBstAPIAc5 promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgaatgaatatttgcactattactattcaaaactattaagatagcaatcacattcaatagccaaatactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaaatgctgcatatttaggacggcatcattatagaaatgcttcttgctgtgtacttttctctcgtcacttacttataaacgtgataatgataagttttgataattctatcgttagtgtaagttatcggtttatgatatggtggactcgctacgttgctttactagttaaactcgtttttacgacgtataaatcctgccgtagtaatatctttacgaagaacgacacatgaaaagagagcagBsaBIAc5 promoter1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tggcagctgtttcgccgttattgttaaaaccggcttaagttaggtgtgttttctacgactagtgaatgccctactagaagatgtgtgttgcacaaaatgtccctggaataaccaatttgaagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatgtttaccgtcgacaaagcggcaataacaattttggccgaattcaatccacacaaaagatgctgatcacttacgggatgatcttctacacacaacgtgttttacagggaccttattggttaaacttcacgtctatcgtcatttgcattcgattatacttataataaattgacattacaaaAflIIAc5 promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    taatatcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcatacaatgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagataaccagctgaagtatcgaatatgagtaacccccaaattgaatcacatgccgcattatagcgacctgtaatgattatttgggtgatatttgtgtacatgtatacatacaaaaccgtatgttactcatcaaccccttttttacacattttcgtggcactggtagtgtcgtatttctattggtcgacttcatagcttatactcattgggggtttaacttagtgtacggcgPciIAc5 promoter2,1202,1402,1602,1802,2002,2202,2402,260
    aactgataggacccatggaagtacactcttcatggcgatatacaagacacacacaagcacgaacacccagttgcggaggaaattctccgtaaatgaaaacccaatcggcgaacaattcatacccatatatggtaaaagttttgaacgcgacttgagagcggagagcattgcggctttgactatcctgggtaccttcatgtgagaagtaccgctatatgttctgtgtgtgttcgtgcttgtgggtcaacgcctcctttaagaggcatttacttttgggttagccgcttgttaagtatgggtatataccattttcaaaacttgcgctgaactctcgcctctcgtaacgccgaPsrIAc5 promoter2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gataaggttttagcgctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccgtttgagttcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtctaatccagagaccccggatcggggtacctactagtccagtgtctattccaaaatcgcgattcgcccgaaatattttgcccgacgccctggtcaaaagtatagtgatggcaaactcaagaacacgacacacctatgaggagggctgtgtttcggcgaggtagtcggtcgtcagcagattaggtctctggggcctagccccatggatgatcaggtcacaAfeIBlpIKpnIAcc65IAc5 promoter2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggtggaattccgaaatgggatcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCccaccttaaggctttaccctagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGBstXIEcoRIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32IE34GE36GE38GR40PY42EG44TQ46TA482657-3364mCherry2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCBsaAIBstEIIKL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90FP92EG94FK96WE98RV100MN102FE104DG1062657-3364mCherry2,8202,8402,8602,8802,9002,9202,9402,960
    GCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAAloIPstISbfIBsaXIPflMIBstEIIGV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150SE152RM154YP156ED158GA160LK162GE164I2657-3364mCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGBbvCIBsaXIK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208NE210DY212TI214VE216QY218ER220AE222G2657-3364mCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACKpnIMluIXcmIBamHISgrAIAcc65ID1A2L3S1V2*3V4G53485-3595G1T2R224HS226TG228GM230DE232LY234KS2362657-3364E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333374-3472G1S25' StopGo (2A; Loughran 2017)mCherry5' stemADAR ...rate3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAAgeIBaeIBfuAIBspMIPaqCIG6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T373485-3595E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L233608-3706T1G2T1S2ADAR substrate3' StopGo (2A; Loughran 2017)3xMS2 SGRloop13' stemloop2loop33,5203,5403,5603,5803,6003,6203,6403,660
    CGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCACAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGPfoITliIXhoIPspXIPaeR7IVS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32GE34GD36AT38YG40KL42TL44KF463713-4426A24G25D26V27E28S29N30P31G32P333608-3706S1S2GFP3' StopGo (2...ghran 2017)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAATATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTBarIBsaAIBstXISnaBIIC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98FF100KD102DG1043713-4426GFP3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCANY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150YI152MA154DK156QK158NG160IK162V3713-4426GFP4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTTTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAAScaIN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220L3713-4426GFP4,2204,2404,2604,2804,3004,3204,3404,360
    GAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggcggccgctcgagtctagagggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattgagtttaaacccgctgCTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccgccggcgagctcagatctcccgggcgccaagcttccattcggatagggattgggagaggagccagagctaagatgcgcatggccagtagtagtggtagtggtaactcaaatttgggcgacAgeIApaIEagIMluINotIPmeITliIXbaIXhoIBstBIPspXISacIIPaeR7IPspOMIE222FV224TA226AG228IT230LG232MD234EL236YK2383713-4426*1G1K2P3I4P5N6P7L8L9G10L11D12S13T14V5 tagGFPi don't think i need this4,3804,4004,4204,4404,4604,4804,5004,5204,540
    atcagcctcgactgtgccttctaaggcctgagctcgctgatcagcctcgatcgaggatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctagtcggagctgacacggaagattccggactcgagcgactagtcggagctagctcctaggtctgtactattctatgtaactactcaaacctgtttggtgttgatcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgSacIBamHIEco53kID1P2D3M4I5R6Y7I8D9E10F11G12Q13T14T15T16R17M18Q19VP1_SV40 (fragment)SV40 poly(A) signal4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgacgttatttgttcaattgttgttgttaacgtaagtaaaatacaaagtccaagtccccctccacaccctccaaaaaatttcgttcattttggagatgtttacaccataccgactaatactagtcagctggctacgggaactctcggaagttgggtcagtcgaggaaggccacccgcHpaISalIBsaBIVD2RC4P*6EP8ST10QS12AP14SG16GR18TcR (fragment)I28N27*26T25*24P23S22T21H20S19T18K17*16L15L14Y13F12R11*10L9H8P7I6A5S4*3S2*1large T antigen (fragment)SV40...nal4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagcctgccccgtactgatagcagcggcgtgaatactgacagaagaaatagtacgttgagcatcctgtccacggccgtcgcgagacccagtaaaagccgctcctggcgaaagcgacctcgcgctgctactagccggacagcgaacgccataagccttagaacgtgcgggagcgagttcggaAfeIBpmINaeIPshAINgoMIVGA20*L22SS24PH26L*28LS30SL32SC34NS36*D38RC40RQ42RS44GS46FS48AR50TA52FA54GA56RR58*S60AC62RL64RY66SE68SC70TP72SL74KP76TcR (fragment)4,9204,9404,9604,9805,0005,0205,0405,060
    tcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatagcagtgaccagggcggtggtttgcaaagccgctcttcgtccggtaatagcggccgtaccgccggctgcgcgacccgatgcagaacgaccgcaagcgctgcgctccgacctaccggaaggggtaatactaagaagagcgaaggccgccgtagccctacgggcgcaacgtccggtaBglIEagINaeINruIBstAPINgoMIVSS78LV80PP82PN84VS86AR88SR90PL92SP94AW96RP98TR100WA102TS104CW106RS108RR110EA112GW114PS116PL118*F120FS122LP124AA126SG128CP130RC132RP134TcR (fragment)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gctgtccaggcaggtagatgacgaccatcagggacagcttcaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagacgacaggtccgtccatctactgctggtagtccctgtcgaagttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctBfuAIBspMICC136PG138R*140MT142TI144RD146SF148KTcR (fragment)ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacacttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgori5,6205,6405,6605,6805,7005,7205,7405,760
    tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagaoriRNAI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcactgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtg*W286HK284IL282SA280GAmpR: Region 15,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctBglIBpmII278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SAmpR: Region 16,1406,1606,1806,2006,2206,2406,2606,2806,300
    gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgacgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttAseIFspIPstIR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AA182PM180TT178DR176ED174NP172IA170EN168LE166PE164WAmpR: Region 16,3206,3406,3606,3806,4006,4206,4406,460
    cgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtggctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccacR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104AmpR: Region 16,4806,5006,5206,5406,5606,5806,6006,6206,640
    agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttctcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttgtgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagScaIYE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DV82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46AmpR: Region 16,6606,6806,7006,7206,7406,7606,7806,8006,820
    gatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggtctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaIY44GV42RA40AmpR: Region 1GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2MAmpR: Region 2AmpR promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctatataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagattctttggtaataatagtactgtaattggataAmpR promoter7,0207,0407,0607,0807,1007,120
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    7129 bp500055006000650050010001500Ac5 promoteroriRNAIAmpR: Region 1AmpR: Region 2Che_5 (DNA)EKo438.4 (DNA)oAPL0131 (DNA)oAPL0126 (DNA)oAPL0122 (DNA)AseI,BglIIPflMI,AsiSI,TspMI,XmaI,SmaIBbvCINruIPciISphICspCINsiIFspIBstAPI,BspDI,ClaIBpmIBglI,AseIFspIPstIScaIAmpR promoter

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